Restriction map for
pF1KB8905
(Circular) (Six-base) MAPSORT of: pF1KB8905.seq Check: 6235 from: 1 to: 3824
pF1KB8905 3824 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 840 840
Size: 3824
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 404 607 628 1649 2081 2828 3096 249
Size: 155 203 21 1021 432 747 268 977
Fragments arranged by size:
1021 977 747 432 268 203 155 21
AflIII A'CryG_T
Cuts at: 1176 2280 3257 1176
Size: 1104 977 1743
Fragments arranged by size:
1743 1104 977
AgeI A'CCGG_T
Cuts at: 2212 2212
Size: 3824
AlwNI CAG_nnn'CTG
Cuts at: 351 424 1129 2696 351
Size: 73 705 1567 1479
Fragments arranged by size:
1567 1479 705 73
ApaI G_GGCC'C
Cuts at: 418 437 418
Size: 19 3805
ApaLI G'TGCA_C
Cuts at: 2594 2594
Size: 3824
ApoI r'AATT_y
Cuts at: 828 3177 3433 828
Size: 2349 256 1219
Fragments arranged by size:
2349 1219 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 734 777 787 844 734
Size: 43 10 57 3714
Fragments arranged by size:
3714 57 43 10
BamHI G'GATC_C
Cuts at: 849 849
Size: 3824
BanI G'GyrC_C
Cuts at: 298 596 840 1254 1502 1537 298
Size: 298 244 414 248 35 2585
Fragments arranged by size:
2585 414 298 248 244 35
BanII G_rGCy'C
Cuts at: 229 418 437 460 477 838 1868 229
Size: 189 19 23 17 361 1030 2185
Fragments arranged by size:
2185 1030 361 189 23 19 17
BbeI G_GCGC'C
Cuts at: 600 1258 1506 600
Size: 658 248 2918
Fragments arranged by size:
2918 658 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 513 547 616 650 513
Size: 34 69 34 3687
Fragments arranged by size:
3687 69 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1722 2483 3824 1722
Size: 761 1341 1722
Fragments arranged by size:
1722 1341 761
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglI GCCn_nnn'nGGC
Cuts at: 203 688 721 730 203
Size: 485 33 9 3297
Fragments arranged by size:
3297 485 33 9
BglII A'GATC_T
Cuts at: 1339 3376 1339
Size: 2037 1787
BlpI GC'TnA_GC
Cuts at: 936 936
Size: 3824
Bme1580I G_kGCm'C
Cuts at: 301 418 437 1449 1542 2598 301
Size: 117 19 1012 93 1056 1527
Fragments arranged by size:
1527 1056 1012 117 93 19
BmgBI CAC'GTC
Cuts at: 483 483
Size: 3824
BmrI ACTGGGnnnn_n'
Cuts at: 232 412 1036 1117 1452 3596 232
Size: 180 624 81 335 2144 460
Fragments arranged by size:
2144 624 460 335 180 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 3824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 688 334
Size: 354 3470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1879 2371 2669 1000
Size: 879 492 298 2155
Fragments arranged by size:
2155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 525 3528 525
Size: 3003 821
BsaAI yAC'GTr
Cuts at: 1177 1177
Size: 3824
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 597 1255 1503 2205 3701 597
Size: 658 248 702 1496 720
Fragments arranged by size:
1496 720 702 658 248
BsaWI w'CCGG_w
Cuts at: 1013 1534 2212 2486 2633 3044 1013
Size: 521 678 274 147 411 1793
Fragments arranged by size:
1793 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 307
Size: 30 3794
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 478 331
Size: 147 3677
BseYI C'CCAG_C
Cuts at: 121 351 358 476 695 1249 2007 2584 3080
Size: 230 7 118 219 554 758 577 496
Cuts at: 3080 121
Size: 865
Fragments arranged by size:
865 758 577 554 496 230 219 118
7
BsiEI CG_ry'CG
Cuts at: 104 1366 1412 2620 104
Size: 1262 46 1208 1308
Fragments arranged by size:
1308 1262 1208 46
BsiHKAI G_wGCw'C
Cuts at: 838 1616 1806 2598 838
Size: 778 190 792 2064
Fragments arranged by size:
2064 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 229 301 418 437 460 477 838 1449 1542
Size: 72 117 19 23 17 361 611 93
Cuts at: 1542 1616 1806 1868 2598 229
Size: 74 190 62 730 1455
Fragments arranged by size:
1455 730 611 361 190 117 93 74
72 62 23 19 17
BspEI T'CCGG_A
Cuts at: 3044 3044
Size: 3824
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 874 1088 1390 1771 874
Size: 214 302 381 2927
Fragments arranged by size:
2927 381 302 214
BsrBI CCG'CTC
Cuts at: 50 498 782 2116 2170 3654 3819 50
Size: 448 284 1334 54 1484 165 55
Fragments arranged by size:
1484 1334 448 284 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1736 1736
Size: 3824
BsrFI r'CCGG_y
Cuts at: 545 767 1822 2003 2212 545
Size: 222 1055 181 209 2157
Fragments arranged by size:
2157 1055 222 209 181
BssHII G'CGCG_C
Cuts at: 678 678
Size: 3824
BssSI C'ACGA_G
Cuts at: 2095 2453 2095
Size: 358 3466
BstAPI GCAn_nnn'nTGC
Cuts at: 1201 1201
Size: 3824
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstXI CCAn_nnnn'nTGG
Cuts at: 129 129
Size: 3824
BstYI r'GATC_y
Cuts at: 1 849 1316 1339 1674 1920 2932 3018 3030
Size: 848 467 23 335 246 1012 86 12
Cuts at: 3030 3376 1
Size: 346 449
Fragments arranged by size:
1012 848 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 134 392 438 615 107
Size: 27 258 46 177 3316
Fragments arranged by size:
3316 258 177 46 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 1067 1067
Size: 3824
DraI TTT'AAA
Cuts at: 823 823
Size: 3824
DrdI GACnn_nn'nnGTC
Cuts at: 1530 2388 1530
Size: 858 2966
EaeI y'GGCC_r
Cuts at: 131 304 689 1409 1583 1974 2001 3346 131
Size: 173 385 720 174 391 27 1345 609
Fragments arranged by size:
1345 720 609 391 385 174 173 27
EagI C'GGCC_G
Cuts at: 1409 1409
Size: 3824
EarI CTCTTCn'nnn_
Cuts at: 380 817 1847 2057 380
Size: 437 1030 210 2147
Fragments arranged by size:
2147 1030 437 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 2342 2488 3634 2032
Size: 310 146 1146 2222
Fragments arranged by size:
2222 1146 310 146
EcoICRI GAG'CTC
Cuts at: 836 836
Size: 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 334 404 607 628 688 1649 2081 2828
Size: 85 70 203 21 60 961 432 747
Cuts at: 2828 3096 249
Size: 268 977
Fragments arranged by size:
977 961 747 432 268 203 85 70
60 21
EcoO109I rG'GnC_Cy
Cuts at: 414 434 798 963 414
Size: 20 364 165 3275
Fragments arranged by size:
3275 364 165 20
EcoRI G'AATT_C
Cuts at: 828 828
Size: 3824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 1605 1605
Size: 3824
HaeII r_GCGC'y
Cuts at: 517 580 600 1258 1506 2528 3507 517
Size: 63 20 658 248 1022 979 834
Fragments arranged by size:
1022 979 834 658 248 63 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1666 1696 1698 1728 1666
Size: 30 2 30 3762
Fragments arranged by size:
3762 30 30 2
HincII GTy'rAC
Cuts at: 863 863
Size: 3824
KasI G'GCGC_C
Cuts at: 596 1254 1502 596
Size: 658 248 2918
Fragments arranged by size:
2918 658 248
KpnI G_GTAC'C
Cuts at: 844 844
Size: 3824
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 530 2495 2679 530
Size: 1965 184 1675
Fragments arranged by size:
1965 1675 184
MscI TGG'CCA
Cuts at: 133 1585 133
Size: 1452 2372
MslI CAynn'nnrTG
Cuts at: 1813 1940 1813
Size: 127 3697
MspA1I CmG'CkG
Cuts at: 351 489 552 617 657 699 935 1249 1609
Size: 138 63 65 40 42 236 314 360
Cuts at: 1609 2622 2867 351
Size: 1013 245 1308
Fragments arranged by size:
1308 1013 360 314 245 236 138 65
63 42 40
NaeI GCC'GGC
Cuts at: 2005 2005
Size: 3824
NarI GG'CG_CC
Cuts at: 597 1255 1503 597
Size: 658 248 2918
Fragments arranged by size:
2918 658 248
NcoI C'CATG_G
Cuts at: 107 134 392 438 107
Size: 27 258 46 3493
Fragments arranged by size:
3493 258 46 27
NgoMIV G'CCGG_C
Cuts at: 2003 2003
Size: 3824
NspI r_CATG'y
Cuts at: 877 1377 2284 877
Size: 500 907 2417
Fragments arranged by size:
2417 907 500
PasI CC'CwG_GG
Cuts at: 451 451
Size: 3824
PciI A'CATG_T
Cuts at: 2280 2280
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3792
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 3824
PspOMI G'GGCC_C
Cuts at: 414 433 414
Size: 19 3805
PspXI vC'TCGA_Gb
Cuts at: 777 777
Size: 3824
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 604 636 604
Size: 32 3792
PstI C_TGCA'G
Cuts at: 344 407 653 871 1556 344
Size: 63 246 218 685 2612
Fragments arranged by size:
2612 685 246 218 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3824
PvuII CAG'CTG
Cuts at: 351 489 552 699 1249 1609 351
Size: 138 63 147 550 360 2566
Fragments arranged by size:
2566 550 360 147 138 63
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 838 838
Size: 3824
SacII CC_GC'GG
Cuts at: 618 618
Size: 3824
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SanDI GG'GwC_CC
Cuts at: 798 798
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 212 340 403 649 867 1552 2545 2736
Size: 179 128 63 246 218 685 993 191
Cuts at: 2736 33
Size: 1121
Fragments arranged by size:
1121 993 685 246 218 191 179 128
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 203 721 730 203
Size: 518 9 3297
Fragments arranged by size:
3297 518 9
SfoI GGC'GCC
Cuts at: 598 1256 1504 598
Size: 658 248 2918
Fragments arranged by size:
2918 658 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 736 789 846 736
Size: 53 57 3714
Fragments arranged by size:
3714 57 53
SmlI C'TyrA_G
Cuts at: 777 979 1894 2386 2648 777
Size: 202 915 492 262 1953
Fragments arranged by size:
1953 915 492 262 202
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 877 1377 877
Size: 500 3324
StuI AGG'CCT
Cuts at: 311 631 311
Size: 320 3504
StyI C'CwwG_G
Cuts at: 107 134 392 438 958 1312 107
Size: 27 258 46 520 354 2619
Fragments arranged by size:
2619 520 354 258 46 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 273 1407 1540 1999 2208 273
Size: 1134 133 459 209 1889
Fragments arranged by size:
1889 1134 459 209 133
TatI w'GTAC_w
Cuts at: 323 3339 323
Size: 3016 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1319 1319
Size: 3824
XhoI C'TCGA_G
Cuts at: 777 777
Size: 3824
XmaI C'CCGG_G
Cuts at: 734 787 844 734
Size: 53 57 3714
Fragments arranged by size:
3714 57 53
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI SexAI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI