Restriction map for pF1KB8908
 (Circular) (Six-base) MAPSORT of: pF1KB8908.seq  Check: 4151  from: 1  to: 3839



pF1KB8908  3839 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:    877     877 
   Size:       3839
 
Acc65I G'GTAC_C

Cuts at:    855     855 
   Size:       3839
 
AclI AA'CG_TT

Cuts at:   3679    3679 
   Size:       3839
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1664    2096    2843    3111    1664 
   Size:        432     747     268    2392

  Fragments arranged by size: 

                2392     747     432     268
 
AflIII A'CryG_T

Cuts at:   1191    2295    3272    1191 
   Size:       1104     977    1758

  Fragments arranged by size: 

                1758    1104     977
 
AgeI A'CCGG_T

Cuts at:   2227    2227 
   Size:       3839
 
AlwNI CAG_nnn'CTG

Cuts at:   1144    2711    1144 
   Size:       1567    2272
 
ApaI G_GGCC'C

Cuts at:    233     233 
   Size:       3839
 
ApaLI G'TGCA_C

Cuts at:   2609    2609 
   Size:       3839
 
ApoI r'AATT_y

Cuts at:    367     843    3192    3448     367 
   Size:        476    2349     256     758

  Fragments arranged by size: 

                2349     758     476     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3839
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3839
 
AvaI C'yCGr_G

Cuts at:    755     859     755 
   Size:        104    3735
 
BamHI G'GATC_C

Cuts at:    864     864 
   Size:       3839
 
BanI G'GyrC_C

Cuts at:    243     295     855    1269    1517    1552     243 
   Size:         52     560     414     248      35    2530

  Fragments arranged by size: 

                2530     560     414     248      52      35
 
BanII G_rGCy'C

Cuts at:    115     233     414     756     853    1883     115 
   Size:        118     181     342      97    1030    2071

  Fragments arranged by size: 

                2071    1030     342     181     118      97
 
BbeI G_GCGC'C

Cuts at:    247    1273    1521     247 
   Size:       1026     248    2565

  Fragments arranged by size: 

                2565    1026     248
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    414     448     414 
   Size:         34    3805
 
BciVI GTATCCnnnnn_n'

Cuts at:   1737    2498    3839    1737 
   Size:        761    1341    1737

  Fragments arranged by size: 

                1737    1341     761
 
BclI T'GATC_A

Cuts at:   1359    1359 
   Size:       3839
 
BglII A'GATC_T

Cuts at:    689    1354    3391     689 
   Size:        665    2037    1137

  Fragments arranged by size: 

                2037    1137     665
 
BlpI GC'TnA_GC

Cuts at:    343     413     951     343 
   Size:         70     538    3231

  Fragments arranged by size: 

                3231     538      70
 
Bme1580I G_kGCm'C

Cuts at:    233    1464    1557    2613     233 
   Size:       1231      93    1056    1459

  Fragments arranged by size: 

                1459    1231    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1051    1132    1467    3611    1051 
   Size:         81     335    2144    1279

  Fragments arranged by size: 

                2144    1279     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2206    2206 
   Size:       3839
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1015    1894    2386    2684    1015 
   Size:        879     492     298    2170

  Fragments arranged by size: 

                2170     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    528    3543     528 
   Size:       3015     824
 
BsaAI yAC'GTr

Cuts at:   1192    1192 
   Size:       3839
 
BsaBI GATnn'nnATC

Cuts at:      6    1353    3390       6 
   Size:       1347    2037     455

  Fragments arranged by size: 

                2037    1347     455
 
BsaHI Gr'CG_yC

Cuts at:    244     359    1270    1518    2220    3716     244 
   Size:        115     911     248     702    1496     367

  Fragments arranged by size: 

                1496     911     702     367     248     115
 
BsaWI w'CCGG_w

Cuts at:   1028    1549    2227    2501    2648    3059    1028 
   Size:        521     678     274     147     411    1808

  Fragments arranged by size: 

                1808     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    283     313     543     573     283 
   Size:         30     230      30    3549

  Fragments arranged by size: 

                3549     230      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    778     811     823     778 
   Size:         33      12    3794

  Fragments arranged by size: 

                3794      33      12
 
BseYI C'CCAG_C

Cuts at:    511    1264    2022    2599    3095     511 
   Size:        753     758     577     496    1255

  Fragments arranged by size: 

                1255     758     753     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1381    1427    2635     104 
   Size:       1277      46    1208    1308

  Fragments arranged by size: 

                1308    1277    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    115     340     853    1631    1821    2613     115 
   Size:        225     513     778     190     792    1341

  Fragments arranged by size: 

                1341     792     778     513     225     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    350     512     350 
   Size:        162    3677
 
Bsp1286I G_dGCh'C

Cuts at:    115     233     340     414     756     853    1464    1557    1631 
   Size:        118     107      74     342      97     611      93      74

Cuts at:   1631    1821    1883    2613     115 
   Size:        190      62     730    1341

  Fragments arranged by size: 

                1341     730     611     342     190     118     107      97
                  93      74      74      62
 
BspEI T'CCGG_A

Cuts at:   3059    3059 
   Size:       3839
 
BspHI T'CATG_A

Cuts at:   3015    3015 
   Size:       3839
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    889    1103    1405    1786     889 
   Size:        214     302     381    2942

  Fragments arranged by size: 

                2942     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2131    2185    3669    3834      50 
   Size:       2081      54    1484     165      55

  Fragments arranged by size: 

                2081    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1751    1751 
   Size:       3839
 
BsrFI r'CCGG_y

Cuts at:    151     168     185     578    1837    2018    2227     151 
   Size:         17      17     393    1259     181     209    1763

  Fragments arranged by size: 

                1763    1259     393     209     181      17      17
 
BssSI C'ACGA_G

Cuts at:   2110    2468    2110 
   Size:        358    3481
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1216    1216 
   Size:       3839
 
BstBI TT'CG_AA

Cuts at:   2200    2200 
   Size:       3839
 
BstYI r'GATC_y

Cuts at:      1     689     864    1331    1354    1689    1935    2947    3033 
   Size:        688     175     467      23     335     246    1012      86

Cuts at:   3033    3045    3391       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     688     467     449     346     335     246     175
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    291     351     526     291 
   Size:         60     175    3604

  Fragments arranged by size: 

                3604     175      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1789    1970    2133    1789 
   Size:        181     163    3495

  Fragments arranged by size: 

                3495     181     163
 
BtsI GCAGTG_nn'

Cuts at:    564    1082     564 
   Size:        518    3321
 
DraI TTT'AAA

Cuts at:    838     838 
   Size:       3839
 
DrdI GACnn_nn'nnGTC

Cuts at:   1545    2403    1545 
   Size:        858    2981
 
EaeI y'GGCC_r

Cuts at:   1424    1598    1989    2016    3361    1424 
   Size:        174     391      27    1345    1902

  Fragments arranged by size: 

                1902    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1424    1424 
   Size:       3839
 
EarI CTCTTCn'nnn_

Cuts at:    788     800    1862    2072     788 
   Size:         12    1062     210    2555

  Fragments arranged by size: 

                2555    1062     210      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    447    2047    2357    2503    3649     447 
   Size:       1600     310     146    1146     637

  Fragments arranged by size: 

                1600    1146     637     310     146
 
EcoICRI GAG'CTC

Cuts at:    113     851     113 
   Size:        738    3101
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1664    2096    2843    3111    1664 
   Size:        432     747     268    2392

  Fragments arranged by size: 

                2392     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    978     978 
   Size:       3839
 
EcoRI G'AATT_C

Cuts at:    843     843 
   Size:       3839
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1858    1858 
   Size:       3839
 
FspI TGC'GCA

Cuts at:   1620    1620 
   Size:       3839
 
HaeII r_GCGC'y

Cuts at:    247     388    1273    1521    2543    3522     247 
   Size:        141     885     248    1022     979     564

  Fragments arranged by size: 

                1022     979     885     564     248     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    436     468    1681    1711    1713    1743     436 
   Size:         32    1213      30       2      30    2532

  Fragments arranged by size: 

                2532    1213      32      30      30       2
 
HincII GTy'rAC

Cuts at:    126     878     126 
   Size:        752    3087
 
KasI G'GCGC_C

Cuts at:    243    1269    1517     243 
   Size:       1026     248    2565

  Fragments arranged by size: 

                2565    1026     248
 
KpnI G_GTAC'C

Cuts at:    859     859 
   Size:       3839
 
MluI A'CGCG_T

Cuts at:   3272    3272 
   Size:       3839
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    335     622    2510    2694     335 
   Size:        287    1888     184    1480

  Fragments arranged by size: 

                1888    1480     287     184
 
MscI TGG'CCA

Cuts at:   1600    1600 
   Size:       3839
 
MslI CAynn'nnrTG

Cuts at:   1828    1955    1828 
   Size:        127    3712
 
MspA1I CmG'CkG

Cuts at:    950    1264    1624    2637    2882     950 
   Size:        314     360    1013     245    1907

  Fragments arranged by size: 

                1907    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    153    2020     153 
   Size:       1867    1972
 
NarI GG'CG_CC

Cuts at:    244    1270    1518     244 
   Size:       1026     248    2565

  Fragments arranged by size: 

                2565    1026     248
 
NcoI C'CATG_G

Cuts at:    526     526 
   Size:       3839
 
NgoMIV G'CCGG_C

Cuts at:    151    2018     151 
   Size:       1867    1972
 
NspI r_CATG'y

Cuts at:    892    1392    2299     892 
   Size:        500     907    2432

  Fragments arranged by size: 

                2432     907     500
 
PasI CC'CwG_GG

Cuts at:    663     663 
   Size:       3839
 
PciI A'CATG_T

Cuts at:   2295    2295 
   Size:       3839
 
PflMI CCAn_nnn'nTGG

Cuts at:    219     219 
   Size:       3839
 
PmeI GTTT'AAAC

Cuts at:    838     838 
   Size:       3839
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    322     354    2978    3010     322 
   Size:         32    2624      32    1151

  Fragments arranged by size: 

                2624    1151      32      32
 
PspOMI G'GGCC_C

Cuts at:    229     229 
   Size:       3839
 
PstI C_TGCA'G

Cuts at:    886    1571     886 
   Size:        685    3154
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3839
 
PvuII CAG'CTG

Cuts at:   1264    1624    1264 
   Size:        360    3479
 
RsrII CG'GwC_CG

Cuts at:   2034    2034 
   Size:       3839
 
SacI G_AGCT'C

Cuts at:    115     853     115 
   Size:        738    3101
 
SalI G'TCGA_C

Cuts at:    876     876 
   Size:       3839
 
SapI GCTCTTCn'nnn_

Cuts at:   1862    2072    1862 
   Size:        210    3629
 
SbfI CC_TGCA'GG

Cuts at:    886     886 
   Size:       3839
 
ScaI AGT'ACT

Cuts at:   3356    3356 
   Size:       3839
 
SfcI C'TryA_G

Cuts at:     33     882    1567    2560    2751      33 
   Size:        849     685     993     191    1121

  Fragments arranged by size: 

                1121     993     849     685     191
 
SfoI GGC'GCC

Cuts at:    245    1271    1519     245 
   Size:       1026     248    2565

  Fragments arranged by size: 

                2565    1026     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3839
 
SmaI CCC'GGG

Cuts at:    861     861 
   Size:       3839
 
SmlI C'TyrA_G

Cuts at:    994    1909    2401    2663     994 
   Size:        915     492     262    2170

  Fragments arranged by size: 

                2170     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3839
 
SphI G_CATG'C

Cuts at:    892    1392     892 
   Size:        500    3339
 
StyI C'CwwG_G

Cuts at:    526     590     973    1327     526 
   Size:         64     383     354    3038

  Fragments arranged by size: 

                3038     383     354      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    688    1422    1555    2014    2223     688 
   Size:        734     133     459     209    2304

  Fragments arranged by size: 

                2304     734     459     209     133
 
TatI w'GTAC_w

Cuts at:   3354    3354 
   Size:       3839
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    283     315     543     575    3356    3388     283 
   Size:         32     228      32    2781      32     734

  Fragments arranged by size: 

                2781     734     228      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1636    1636 
   Size:       3839
 
XbaI T'CTAG_A

Cuts at:    870     870 
   Size:       3839
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1334    1334 
   Size:       3839
 
XmaI C'CCGG_G

Cuts at:    859     859 
   Size:       3839
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I     BsgI    BsiWI     BsmI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PfoI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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