(Circular) (Six-base) MAPSORT of: pF1KB8908.seq Check: 4151 from: 1 to: 3839 pF1KB8908 3839 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 877 877 Size: 3839 Acc65I G'GTAC_C Cuts at: 855 855 Size: 3839 AclI AA'CG_TT Cuts at: 3679 3679 Size: 3839 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1664 2096 2843 3111 1664 Size: 432 747 268 2392 Fragments arranged by size: 2392 747 432 268 AflIII A'CryG_T Cuts at: 1191 2295 3272 1191 Size: 1104 977 1758 Fragments arranged by size: 1758 1104 977 AgeI A'CCGG_T Cuts at: 2227 2227 Size: 3839 AlwNI CAG_nnn'CTG Cuts at: 1144 2711 1144 Size: 1567 2272 ApaI G_GGCC'C Cuts at: 233 233 Size: 3839 ApaLI G'TGCA_C Cuts at: 2609 2609 Size: 3839 ApoI r'AATT_y Cuts at: 367 843 3192 3448 367 Size: 476 2349 256 758 Fragments arranged by size: 2349 758 476 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3839 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3839 AvaI C'yCGr_G Cuts at: 755 859 755 Size: 104 3735 BamHI G'GATC_C Cuts at: 864 864 Size: 3839 BanI G'GyrC_C Cuts at: 243 295 855 1269 1517 1552 243 Size: 52 560 414 248 35 2530 Fragments arranged by size: 2530 560 414 248 52 35 BanII G_rGCy'C Cuts at: 115 233 414 756 853 1883 115 Size: 118 181 342 97 1030 2071 Fragments arranged by size: 2071 1030 342 181 118 97 BbeI G_GCGC'C Cuts at: 247 1273 1521 247 Size: 1026 248 2565 Fragments arranged by size: 2565 1026 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 414 448 414 Size: 34 3805 BciVI GTATCCnnnnn_n' Cuts at: 1737 2498 3839 1737 Size: 761 1341 1737 Fragments arranged by size: 1737 1341 761 BclI T'GATC_A Cuts at: 1359 1359 Size: 3839 BglII A'GATC_T Cuts at: 689 1354 3391 689 Size: 665 2037 1137 Fragments arranged by size: 2037 1137 665 BlpI GC'TnA_GC Cuts at: 343 413 951 343 Size: 70 538 3231 Fragments arranged by size: 3231 538 70 Bme1580I G_kGCm'C Cuts at: 233 1464 1557 2613 233 Size: 1231 93 1056 1459 Fragments arranged by size: 1459 1231 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1051 1132 1467 3611 1051 Size: 81 335 2144 1279 Fragments arranged by size: 2144 1279 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 3839 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1015 1894 2386 2684 1015 Size: 879 492 298 2170 Fragments arranged by size: 2170 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 528 3543 528 Size: 3015 824 BsaAI yAC'GTr Cuts at: 1192 1192 Size: 3839 BsaBI GATnn'nnATC Cuts at: 6 1353 3390 6 Size: 1347 2037 455 Fragments arranged by size: 2037 1347 455 BsaHI Gr'CG_yC Cuts at: 244 359 1270 1518 2220 3716 244 Size: 115 911 248 702 1496 367 Fragments arranged by size: 1496 911 702 367 248 115 BsaWI w'CCGG_w Cuts at: 1028 1549 2227 2501 2648 3059 1028 Size: 521 678 274 147 411 1808 Fragments arranged by size: 1808 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 283 313 543 573 283 Size: 30 230 30 3549 Fragments arranged by size: 3549 230 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 778 811 823 778 Size: 33 12 3794 Fragments arranged by size: 3794 33 12 BseYI C'CCAG_C Cuts at: 511 1264 2022 2599 3095 511 Size: 753 758 577 496 1255 Fragments arranged by size: 1255 758 753 577 496 BsiEI CG_ry'CG Cuts at: 104 1381 1427 2635 104 Size: 1277 46 1208 1308 Fragments arranged by size: 1308 1277 1208 46 BsiHKAI G_wGCw'C Cuts at: 115 340 853 1631 1821 2613 115 Size: 225 513 778 190 792 1341 Fragments arranged by size: 1341 792 778 513 225 190 BsmBI CGTCTCn'nnnn_ Cuts at: 350 512 350 Size: 162 3677 Bsp1286I G_dGCh'C Cuts at: 115 233 340 414 756 853 1464 1557 1631 Size: 118 107 74 342 97 611 93 74 Cuts at: 1631 1821 1883 2613 115 Size: 190 62 730 1341 Fragments arranged by size: 1341 730 611 342 190 118 107 97 93 74 74 62 BspEI T'CCGG_A Cuts at: 3059 3059 Size: 3839 BspHI T'CATG_A Cuts at: 3015 3015 Size: 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 889 1103 1405 1786 889 Size: 214 302 381 2942 Fragments arranged by size: 2942 381 302 214 BsrBI CCG'CTC Cuts at: 50 2131 2185 3669 3834 50 Size: 2081 54 1484 165 55 Fragments arranged by size: 2081 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1751 1751 Size: 3839 BsrFI r'CCGG_y Cuts at: 151 168 185 578 1837 2018 2227 151 Size: 17 17 393 1259 181 209 1763 Fragments arranged by size: 1763 1259 393 209 181 17 17 BssSI C'ACGA_G Cuts at: 2110 2468 2110 Size: 358 3481 BstAPI GCAn_nnn'nTGC Cuts at: 1216 1216 Size: 3839 BstBI TT'CG_AA Cuts at: 2200 2200 Size: 3839 BstYI r'GATC_y Cuts at: 1 689 864 1331 1354 1689 1935 2947 3033 Size: 688 175 467 23 335 246 1012 86 Cuts at: 3033 3045 3391 1 Size: 12 346 449 Fragments arranged by size: 1012 688 467 449 346 335 246 175 86 23 12 BtgI C'CryG_G Cuts at: 291 351 526 291 Size: 60 175 3604 Fragments arranged by size: 3604 175 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1789 1970 2133 1789 Size: 181 163 3495 Fragments arranged by size: 3495 181 163 BtsI GCAGTG_nn' Cuts at: 564 1082 564 Size: 518 3321 DraI TTT'AAA Cuts at: 838 838 Size: 3839 DrdI GACnn_nn'nnGTC Cuts at: 1545 2403 1545 Size: 858 2981 EaeI y'GGCC_r Cuts at: 1424 1598 1989 2016 3361 1424 Size: 174 391 27 1345 1902 Fragments arranged by size: 1902 1345 391 174 27 EagI C'GGCC_G Cuts at: 1424 1424 Size: 3839 EarI CTCTTCn'nnn_ Cuts at: 788 800 1862 2072 788 Size: 12 1062 210 2555 Fragments arranged by size: 2555 1062 210 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 447 2047 2357 2503 3649 447 Size: 1600 310 146 1146 637 Fragments arranged by size: 1600 1146 637 310 146 EcoICRI GAG'CTC Cuts at: 113 851 113 Size: 738 3101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1664 2096 2843 3111 1664 Size: 432 747 268 2392 Fragments arranged by size: 2392 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 978 978 Size: 3839 EcoRI G'AATT_C Cuts at: 843 843 Size: 3839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1858 1858 Size: 3839 FspI TGC'GCA Cuts at: 1620 1620 Size: 3839 HaeII r_GCGC'y Cuts at: 247 388 1273 1521 2543 3522 247 Size: 141 885 248 1022 979 564 Fragments arranged by size: 1022 979 885 564 248 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 436 468 1681 1711 1713 1743 436 Size: 32 1213 30 2 30 2532 Fragments arranged by size: 2532 1213 32 30 30 2 HincII GTy'rAC Cuts at: 126 878 126 Size: 752 3087 KasI G'GCGC_C Cuts at: 243 1269 1517 243 Size: 1026 248 2565 Fragments arranged by size: 2565 1026 248 KpnI G_GTAC'C Cuts at: 859 859 Size: 3839 MluI A'CGCG_T Cuts at: 3272 3272 Size: 3839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 335 622 2510 2694 335 Size: 287 1888 184 1480 Fragments arranged by size: 1888 1480 287 184 MscI TGG'CCA Cuts at: 1600 1600 Size: 3839 MslI CAynn'nnrTG Cuts at: 1828 1955 1828 Size: 127 3712 MspA1I CmG'CkG Cuts at: 950 1264 1624 2637 2882 950 Size: 314 360 1013 245 1907 Fragments arranged by size: 1907 1013 360 314 245 NaeI GCC'GGC Cuts at: 153 2020 153 Size: 1867 1972 NarI GG'CG_CC Cuts at: 244 1270 1518 244 Size: 1026 248 2565 Fragments arranged by size: 2565 1026 248 NcoI C'CATG_G Cuts at: 526 526 Size: 3839 NgoMIV G'CCGG_C Cuts at: 151 2018 151 Size: 1867 1972 NspI r_CATG'y Cuts at: 892 1392 2299 892 Size: 500 907 2432 Fragments arranged by size: 2432 907 500 PasI CC'CwG_GG Cuts at: 663 663 Size: 3839 PciI A'CATG_T Cuts at: 2295 2295 Size: 3839 PflMI CCAn_nnn'nTGG Cuts at: 219 219 Size: 3839 PmeI GTTT'AAAC Cuts at: 838 838 Size: 3839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 322 354 2978 3010 322 Size: 32 2624 32 1151 Fragments arranged by size: 2624 1151 32 32 PspOMI G'GGCC_C Cuts at: 229 229 Size: 3839 PstI C_TGCA'G Cuts at: 886 1571 886 Size: 685 3154 PvuI CG_AT'CG Cuts at: 104 104 Size: 3839 PvuII CAG'CTG Cuts at: 1264 1624 1264 Size: 360 3479 RsrII CG'GwC_CG Cuts at: 2034 2034 Size: 3839 SacI G_AGCT'C Cuts at: 115 853 115 Size: 738 3101 SalI G'TCGA_C Cuts at: 876 876 Size: 3839 SapI GCTCTTCn'nnn_ Cuts at: 1862 2072 1862 Size: 210 3629 SbfI CC_TGCA'GG Cuts at: 886 886 Size: 3839 ScaI AGT'ACT Cuts at: 3356 3356 Size: 3839 SfcI C'TryA_G Cuts at: 33 882 1567 2560 2751 33 Size: 849 685 993 191 1121 Fragments arranged by size: 1121 993 849 685 191 SfoI GGC'GCC Cuts at: 245 1271 1519 245 Size: 1026 248 2565 Fragments arranged by size: 2565 1026 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3839 SmaI CCC'GGG Cuts at: 861 861 Size: 3839 SmlI C'TyrA_G Cuts at: 994 1909 2401 2663 994 Size: 915 492 262 2170 Fragments arranged by size: 2170 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3839 SphI G_CATG'C Cuts at: 892 1392 892 Size: 500 3339 StyI C'CwwG_G Cuts at: 526 590 973 1327 526 Size: 64 383 354 3038 Fragments arranged by size: 3038 383 354 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 688 1422 1555 2014 2223 688 Size: 734 133 459 209 2304 Fragments arranged by size: 2304 734 459 209 133 TatI w'GTAC_w Cuts at: 3354 3354 Size: 3839 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 283 315 543 575 3356 3388 283 Size: 32 228 32 2781 32 734 Fragments arranged by size: 2781 734 228 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1636 1636 Size: 3839 XbaI T'CTAG_A Cuts at: 870 870 Size: 3839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1334 1334 Size: 3839 XmaI C'CCGG_G Cuts at: 859 859 Size: 3839 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI