Restriction map for
pF1KB8909
(Circular) (Six-base) MAPSORT of: pF1KB8909.seq Check: 9460 from: 1 to: 3845
pF1KB8909 3845 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 883 883
Size: 3845
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1670 2102 2849 3117 1670
Size: 432 747 268 2398
Fragments arranged by size:
2398 747 432 268
AfeI AGC'GCT
Cuts at: 739 739
Size: 3845
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 697 729 697
Size: 32 3813
AlwNI CAG_nnn'CTG
Cuts at: 150 554 1150 2717 150
Size: 404 596 1567 1278
Fragments arranged by size:
1567 1278 596 404
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 849 3198 3454 849
Size: 2349 256 1240
Fragments arranged by size:
2349 1240 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 255 865 255
Size: 610 3235
BamHI G'GATC_C
Cuts at: 388 453 870 388
Size: 65 417 3363
Fragments arranged by size:
3363 417 65
BanI G'GyrC_C
Cuts at: 177 861 1275 1523 1558 177
Size: 684 414 248 35 2464
Fragments arranged by size:
2464 684 414 248 35
BanII G_rGCy'C
Cuts at: 859 1889 859
Size: 1030 2815
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2504 3845 1743
Size: 761 1341 1743
Fragments arranged by size:
1743 1341 761
BclI T'GATC_A
Cuts at: 803 1365 803
Size: 562 3283
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 544 1470 1563 2619 544
Size: 926 93 1056 1770
Fragments arranged by size:
1770 1056 926 93
BmgBI CAC'GTC
Cuts at: 402 402
Size: 3845
BmrI ACTGGGnnnn_n'
Cuts at: 138 710 1057 1138 1473 3617 138
Size: 572 347 81 335 2144 366
Fragments arranged by size:
2144 572 366 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 515
Size: 3845
Bpu10I CC'TnA_GC
Cuts at: 224 224
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2392 2690 1021
Size: 879 492 298 2176
Fragments arranged by size:
2176 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3549 3549
Size: 3845
BsaAI yAC'GTr
Cuts at: 635 1198 635
Size: 563 3282
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 203 1276 1524 2226 3722 203
Size: 1073 248 702 1496 326
Fragments arranged by size:
1496 1073 702 326 248
BsaWI w'CCGG_w
Cuts at: 198 689 1034 1555 2233 2507 2654 3065 198
Size: 491 345 521 678 274 147 411 978
Fragments arranged by size:
978 678 521 491 411 345 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 475 762 457
Size: 18 287 3540
Fragments arranged by size:
3540 287 18
BseYI C'CCAG_C
Cuts at: 469 796 1270 2028 2605 3101 469
Size: 327 474 758 577 496 1213
Fragments arranged by size:
1213 758 577 496 474 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 372 615 264
Size: 108 243 3494
Fragments arranged by size:
3494 243 108
BsiEI CG_ry'CG
Cuts at: 104 119 814 1387 1433 2641 104
Size: 15 695 573 46 1208 1308
Fragments arranged by size:
1308 1208 695 573 46 15
BsiHKAI G_wGCw'C
Cuts at: 248 859 1637 1827 2619 248
Size: 611 778 190 792 1474
Fragments arranged by size:
1474 792 778 611 190
Bsp1286I G_dGCh'C
Cuts at: 248 544 859 1470 1563 1637 1827 1889 2619
Size: 296 315 611 93 74 190 62 730
Cuts at: 2619 248
Size: 1474
Fragments arranged by size:
1474 730 611 315 296 190 93 74
62
BspEI T'CCGG_A
Cuts at: 198 3065 198
Size: 2867 978
BspHI T'CATG_A
Cuts at: 743 3021 743
Size: 2278 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 895 1109 1411 1792 895
Size: 214 302 381 2948
Fragments arranged by size:
2948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 210 477 665 2137 2191 3675 3840 50
Size: 160 267 188 1472 54 1484 165 55
Fragments arranged by size:
1484 1472 267 188 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 1757 1757
Size: 3845
BsrFI r'CCGG_y
Cuts at: 1843 2024 2233 1843
Size: 181 209 3455
Fragments arranged by size:
3455 209 181
BssHII G'CGCG_C
Cuts at: 464 464
Size: 3845
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 1222
Size: 3845
BstBI TT'CG_AA
Cuts at: 163 2206 163
Size: 2043 1802
BstEII G'GTnAC_C
Cuts at: 234 250 288 234
Size: 16 38 3791
Fragments arranged by size:
3791 38 16
BstXI CCAn_nnnn'nTGG
Cuts at: 837 837
Size: 3845
BstYI r'GATC_y
Cuts at: 1 388 453 870 1337 1360 1695 1941 2953
Size: 387 65 417 467 23 335 246 1012
Cuts at: 2953 3039 3051 3397 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 417 387 346 335 246
86 65 23 12
BtgI C'CryG_G
Cuts at: 113 113
Size: 3845
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 279 1795 1976 2139 279
Size: 1516 181 163 1985
Fragments arranged by size:
1985 1516 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2409 1551
Size: 858 2987
EaeI y'GGCC_r
Cuts at: 116 637 827 1430 1604 1995 2022 3367 116
Size: 521 190 603 174 391 27 1345 594
Fragments arranged by size:
1345 603 594 521 391 190 174 27
EagI C'GGCC_G
Cuts at: 116 1430 116
Size: 1314 2531
EarI CTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 857 857
Size: 3845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 1670 2102 2849 3117 515
Size: 1155 432 747 268 1243
Fragments arranged by size:
1243 1155 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 984 984
Size: 3845
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3845
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 741 826 1279 1527 2549 3528 741
Size: 85 453 248 1022 979 1058
Fragments arranged by size:
1058 1022 979 453 248 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 438 470 1687 1717 1719 1749 373
Size: 32 33 32 1217 30 2 30 2469
Fragments arranged by size:
2469 1217 33 32 32 30 30 2
HincII GTy'rAC
Cuts at: 884 884
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 416 2516 2700 290
Size: 126 2100 184 1435
Fragments arranged by size:
2100 1435 184 126
MscI TGG'CCA
Cuts at: 639 829 1606 639
Size: 190 777 2878
Fragments arranged by size:
2878 777 190
MslI CAynn'nnrTG
Cuts at: 611 1834 1961 611
Size: 1223 127 2495
Fragments arranged by size:
2495 1223 127
MspA1I CmG'CkG
Cuts at: 115 719 726 956 1270 1630 2643 2888 115
Size: 604 7 230 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 604 360 314 245 230 7
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NotI GC'GGCC_GC
Cuts at: 116 116
Size: 3845
NspI r_CATG'y
Cuts at: 898 1398 2305 898
Size: 500 907 2438
Fragments arranged by size:
2438 907 500
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PflMI CCAn_nnn'nTGG
Cuts at: 150 150
Size: 3845
PfoI T'CCnGG_A
Cuts at: 492 492
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PmlI CAC'GTG
Cuts at: 635 635
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3813
PstI C_TGCA'G
Cuts at: 892 1577 892
Size: 685 3160
PvuI CG_AT'CG
Cuts at: 104 814 104
Size: 710 3135
PvuII CAG'CTG
Cuts at: 1270 1630 1270
Size: 360 3485
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 859 859
Size: 3845
SacII CC_GC'GG
Cuts at: 116 116
Size: 3845
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SfcI C'TryA_G
Cuts at: 33 546 888 1573 2566 2757 33
Size: 513 342 685 993 191 1121
Fragments arranged by size:
1121 993 685 513 342 191
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 257 867 257
Size: 610 3235
SmlI C'TyrA_G
Cuts at: 1000 1915 2407 2669 1000
Size: 915 492 262 2176
Fragments arranged by size:
2176 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 898 1398 898
Size: 500 3345
StyI C'CwwG_G
Cuts at: 239 979 1333 239
Size: 740 354 2751
Fragments arranged by size:
2751 740 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1428 1561 2020 2229 1428
Size: 133 459 209 3044
Fragments arranged by size:
3044 459 209 133
TatI w'GTAC_w
Cuts at: 3360 3360
Size: 3845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 1340 306
Size: 1034 2811
XmaI C'CCGG_G
Cuts at: 255 865 255
Size: 610 3235
XmnI GAAnn'nnTTC
Cuts at: 739 739
Size: 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PciI PflMI PfoI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NruI NsiI PacI PasI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI