Restriction map for
pF1KB8910
(Circular) (Six-base) MAPSORT of: pF1KB8910.seq Check: 32 from: 1 to: 3737
pF1KB8910 3737 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 344 775 344
Size: 431 3306
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
AflIII A'CryG_T
Cuts at: 1089 2193 3170 1089
Size: 1104 977 1656
Fragments arranged by size:
1656 1104 977
AgeI A'CCGG_T
Cuts at: 2125 2125
Size: 3737
AlwNI CAG_nnn'CTG
Cuts at: 608 1042 2609 608
Size: 434 1567 1736
Fragments arranged by size:
1736 1567 434
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 741 3090 3346 741
Size: 2349 256 1132
Fragments arranged by size:
2349 1132 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 190 277 757 190
Size: 87 480 3170
Fragments arranged by size:
3170 480 87
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 320 753 1167 1415 1450 320
Size: 433 414 248 35 2607
Fragments arranged by size:
2607 433 414 248 35
BanII G_rGCy'C
Cuts at: 176 278 293 600 751 1781 176
Size: 102 15 307 151 1030 2132
Fragments arranged by size:
2132 1030 307 151 102 15
BbeI G_GCGC'C
Cuts at: 324 1171 1419 324
Size: 847 248 2642
Fragments arranged by size:
2642 847 248
BbsI GAAGACnn'nnnn_
Cuts at: 398 398
Size: 3737
BciVI GTATCCnnnnn_n'
Cuts at: 1635 2396 3737 1635
Size: 761 1341 1635
Fragments arranged by size:
1635 1341 761
BclI T'GATC_A
Cuts at: 1257 1257
Size: 3737
BglII A'GATC_T
Cuts at: 1252 3289 1252
Size: 2037 1700
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 284 1362 1455 2511 284
Size: 1078 93 1056 1510
Fragments arranged by size:
1510 1078 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 949 1030 1365 3509 949
Size: 81 335 2144 1177
Fragments arranged by size:
2144 1177 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 277 292 649 235
Size: 42 15 357 3323
Fragments arranged by size:
3323 357 42 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 913 1792 2284 2582 160
Size: 753 879 492 298 1315
Fragments arranged by size:
1315 879 753 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3441 3441
Size: 3737
BsaAI yAC'GTr
Cuts at: 1090 1090
Size: 3737
BsaBI GATnn'nnATC
Cuts at: 6 1251 3288 6
Size: 1245 2037 455
Fragments arranged by size:
2037 1245 455
BsaHI Gr'CG_yC
Cuts at: 321 1168 1416 2118 3614 321
Size: 847 248 702 1496 444
Fragments arranged by size:
1496 847 702 444 248
BsaWI w'CCGG_w
Cuts at: 926 1447 2125 2399 2546 2957 926
Size: 521 678 274 147 411 1706
Fragments arranged by size:
1706 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 229
Size: 30 3707
BseYI C'CCAG_C
Cuts at: 1162 1920 2497 2993 1162
Size: 758 577 496 1906
Fragments arranged by size:
1906 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 244 422 115
Size: 129 178 3430
Fragments arranged by size:
3430 178 129
BsiEI CG_ry'CG
Cuts at: 104 371 1279 1325 2533 104
Size: 267 908 46 1208 1308
Fragments arranged by size:
1308 1208 908 267 46
BsiHKAI G_wGCw'C
Cuts at: 176 293 751 1529 1719 2511 176
Size: 117 458 778 190 792 1402
Fragments arranged by size:
1402 792 778 458 190 117
Bsp1286I G_dGCh'C
Cuts at: 176 278 284 293 600 751 1362 1455 1529
Size: 102 6 9 307 151 611 93 74
Cuts at: 1529 1719 1781 2511 176
Size: 190 62 730 1402
Fragments arranged by size:
1402 730 611 307 190 151 102 93
74 62 9 6
BspEI T'CCGG_A
Cuts at: 2957 2957
Size: 3737
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 338 787 1001 1303 1684 338
Size: 449 214 302 381 2391
Fragments arranged by size:
2391 449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 230 2029 2083 3567 3732 50
Size: 180 1799 54 1484 165 55
Fragments arranged by size:
1799 1484 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1649 1649
Size: 3737
BsrFI r'CCGG_y
Cuts at: 1735 1916 2125 1735
Size: 181 209 3347
Fragments arranged by size:
3347 209 181
BssSI C'ACGA_G
Cuts at: 2008 2366 2008
Size: 358 3379
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1114
Size: 3737
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 3737
BstYI r'GATC_y
Cuts at: 1 762 1229 1252 1587 1833 2845 2931 2943
Size: 761 467 23 335 246 1012 86 12
Cuts at: 2943 3289 1
Size: 346 449
Fragments arranged by size:
1012 761 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1687 1868 2031 1687
Size: 181 163 3393
Fragments arranged by size:
3393 181 163
BtsI GCAGTG_nn'
Cuts at: 980 980
Size: 3737
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DraIII CAC_nnn'GTG
Cuts at: 138 591 138
Size: 453 3284
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 368 1322 1496 1887 1914 3259 368
Size: 954 174 391 27 1345 846
Fragments arranged by size:
1345 954 846 391 174 27
EagI C'GGCC_G
Cuts at: 368 1322 368
Size: 954 2783
EarI CTCTTCn'nnn_
Cuts at: 247 1760 1970 247
Size: 1513 210 2014
Fragments arranged by size:
2014 1513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1945 2255 2401 3547 1945
Size: 310 146 1146 2135
Fragments arranged by size:
2135 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 291 749 174
Size: 117 458 3162
Fragments arranged by size:
3162 458 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 277 292 649 1562 1994 2741 3009 235
Size: 42 15 357 913 432 747 268 963
Fragments arranged by size:
963 913 747 432 357 268 42 15
EcoO109I rG'GnC_Cy
Cuts at: 605 876 605
Size: 271 3466
EcoRI G'AATT_C
Cuts at: 741 741
Size: 3737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1756 1756
Size: 3737
FspI TGC'GCA
Cuts at: 1518 1518
Size: 3737
HaeII r_GCGC'y
Cuts at: 324 1171 1419 2441 3420 324
Size: 847 248 1022 979 641
Fragments arranged by size:
1022 979 847 641 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 1579 1609 1611 1641 229
Size: 32 1318 30 2 30 2325
Fragments arranged by size:
2325 1318 32 30 30 2
HincII GTy'rAC
Cuts at: 345 776 345
Size: 431 3306
KasI G'GCGC_C
Cuts at: 320 1167 1415 320
Size: 847 248 2642
Fragments arranged by size:
2642 847 248
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2408 2592 2408
Size: 184 3553
MscI TGG'CCA
Cuts at: 1498 1498
Size: 3737
MslI CAynn'nnrTG
Cuts at: 1726 1853 1726
Size: 127 3610
MspA1I CmG'CkG
Cuts at: 848 1162 1522 2535 2780 848
Size: 314 360 1013 245 1805
Fragments arranged by size:
1805 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 321 1168 1416 321
Size: 847 248 2642
Fragments arranged by size:
2642 847 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3737
NdeI CA'TA_TG
Cuts at: 418 418
Size: 3737
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 790 1290 2197 790
Size: 500 907 2330
Fragments arranged by size:
2330 907 500
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PflMI CCAn_nnn'nTGG
Cuts at: 591 591
Size: 3737
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 163 195 2876 2908 163
Size: 32 2681 32 992
Fragments arranged by size:
2681 992 32 32
PpuMI rG'GwC_Cy
Cuts at: 605 605
Size: 3737
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 629 661 629
Size: 32 3705
PstI C_TGCA'G
Cuts at: 784 1469 784
Size: 685 3052
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 1162 1522 1162
Size: 360 3377
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 176 293 751 176
Size: 117 458 3162
Fragments arranged by size:
3162 458 117
SalI G'TCGA_C
Cuts at: 343 774 343
Size: 431 3306
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SexAI A'CCwGG_T
Cuts at: 587 620 632 587
Size: 33 12 3692
Fragments arranged by size:
3692 33 12
SfcI C'TryA_G
Cuts at: 33 780 1465 2458 2649 33
Size: 747 685 993 191 1121
Fragments arranged by size:
1121 993 747 685 191
SfoI GGC'GCC
Cuts at: 322 1169 1417 322
Size: 847 248 2642
Fragments arranged by size:
2642 847 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SmaI CCC'GGG
Cuts at: 279 759 279
Size: 480 3257
SmlI C'TyrA_G
Cuts at: 175 892 1807 2299 2561 175
Size: 717 915 492 262 1351
Fragments arranged by size:
1351 915 717 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3737
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
SrfI GCCC'GGGC
Cuts at: 279 279
Size: 3737
StyI C'CwwG_G
Cuts at: 107 871 1225 107
Size: 764 354 2619
Fragments arranged by size:
2619 764 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 184 1320 1453 1912 2121 184
Size: 1136 133 459 209 1800
Fragments arranged by size:
1800 1136 459 209 133
TatI w'GTAC_w
Cuts at: 3252 3252
Size: 3737
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 1534 1534
Size: 3737
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 673 1232 673
Size: 559 3178
XmaI C'CCGG_G
Cuts at: 277 757 277
Size: 480 3257
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI