Restriction map for
pF1KB8913
(Circular) (Six-base) MAPSORT of: pF1KB8913.seq Check: 3379 from: 1 to: 3872
pF1KB8913 3872 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 701 910 701
Size: 209 3663
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1697 2129 2876 3144 793
Size: 904 432 747 268 1521
Fragments arranged by size:
1521 904 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 469 1177 2744 469
Size: 708 1567 1597
Fragments arranged by size:
1597 1567 708
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 876 3225 3481 876
Size: 2349 256 1267
Fragments arranged by size:
2349 1267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 182 362 892 182
Size: 180 530 3162
Fragments arranged by size:
3162 530 180
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 232 311 888 1302 1550 1585 232
Size: 79 577 414 248 35 2519
Fragments arranged by size:
2519 577 414 248 79 35
BanII G_rGCy'C
Cuts at: 152 886 1916 152
Size: 734 1030 2108
Fragments arranged by size:
2108 1030 734
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BbsI GAAGACnn'nnnn_
Cuts at: 581 581
Size: 3872
BbvCI CC'TCA_GC
Cuts at: 164 164
Size: 3872
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 281 1392 281
Size: 1111 2761
BglI GCCn_nnn'nGGC
Cuts at: 112 160 112
Size: 48 3824
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 1497 1590 2646 1497
Size: 93 1056 2723
Fragments arranged by size:
2723 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 397 1084 1165 1500 3644 397
Size: 687 81 335 2144 625
Fragments arranged by size:
2144 687 625 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 721 2239 721
Size: 1518 2354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 343 736 235
Size: 108 393 3371
Fragments arranged by size:
3371 393 108
Bpu10I CC'TnA_GC
Cuts at: 164 164
Size: 3872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 1048 1927 2419 2717 225
Size: 823 879 492 298 1380
Fragments arranged by size:
1380 879 823 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 526 3576 526
Size: 3050 822
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 772 802 772
Size: 30 3842
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 361 780 160
Size: 201 419 3252
Fragments arranged by size:
3252 419 201
BseYI C'CCAG_C
Cuts at: 1297 2055 2632 3128 1297
Size: 758 577 496 2041
Fragments arranged by size:
2041 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 3872
BsiEI CG_ry'CG
Cuts at: 104 137 1414 1460 2668 104
Size: 33 1277 46 1208 1308
Fragments arranged by size:
1308 1277 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 650 886 1664 1854 2646 650
Size: 236 778 190 792 1876
Fragments arranged by size:
1876 792 778 236 190
BsmI GAATG_Cn'
Cuts at: 569 569
Size: 3872
Bsp1286I G_dGCh'C
Cuts at: 152 650 886 1497 1590 1664 1854 1916 2646
Size: 498 236 611 93 74 190 62 730
Cuts at: 2646 152
Size: 1378
Fragments arranged by size:
1378 730 611 498 236 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 3048 3048
Size: 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 540 922 1136 1438 1819 540
Size: 382 214 302 381 2593
Fragments arranged by size:
2593 382 381 302 214
BsrBI CCG'CTC
Cuts at: 50 189 209 2164 2218 3702 3867 50
Size: 139 20 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 165 139 55 54 20
BsrDI GCAATG_nn'
Cuts at: 1784 1784
Size: 3872
BsrFI r'CCGG_y
Cuts at: 132 308 1870 2051 2260 132
Size: 176 1562 181 209 1744
Fragments arranged by size:
1744 1562 209 181 176
BssHII G'CGCG_C
Cuts at: 241 241
Size: 3872
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1249
Size: 3872
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstYI r'GATC_y
Cuts at: 1 897 1364 1387 1722 1968 2980 3066 3078
Size: 896 467 23 335 246 1012 86 12
Cuts at: 3078 3424 1
Size: 346 449
Fragments arranged by size:
1012 896 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 252 857 107
Size: 145 605 3122
Fragments arranged by size:
3122 605 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 1115
Size: 3872
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 709 1578 2436 709
Size: 869 858 2145
Fragments arranged by size:
2145 869 858
EaeI y'GGCC_r
Cuts at: 116 134 173 255 1457 1631 2022 2049 3394
Size: 18 39 82 1202 174 391 27 1345
Cuts at: 3394 116
Size: 594
Fragments arranged by size:
1345 1202 594 391 174 82 39 27
18
EagI C'GGCC_G
Cuts at: 134 1457 134
Size: 1323 2549
EarI CTCTTCn'nnn_
Cuts at: 769 1895 2105 769
Size: 1126 210 2536
Fragments arranged by size:
2536 1126 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 2080 2390 2536 3682 360
Size: 1720 310 146 1146 550
Fragments arranged by size:
1720 1146 550 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 343 736 793 1697 2129 2876 3144 235
Size: 108 393 57 904 432 747 268 963
Fragments arranged by size:
963 904 747 432 393 268 108 57
EcoNI CCTnn'n_nnAGG
Cuts at: 718 718
Size: 3872
EcoO109I rG'GnC_Cy
Cuts at: 1011 1011
Size: 3872
EcoRI G'AATT_C
Cuts at: 876 876
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 309 1891 309
Size: 1582 2290
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 322 370 1306 1554 2576 3555 322
Size: 48 936 248 1022 979 639
Fragments arranged by size:
1022 979 936 639 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1714 1744 1746 1776 1714
Size: 30 2 30 3810
Fragments arranged by size:
3810 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MfeI C'AATT_G
Cuts at: 542 542
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 455 551 629 2543 2727 455
Size: 96 78 1914 184 1600
Fragments arranged by size:
1914 1600 184 96 78
MscI TGG'CCA
Cuts at: 1633 1633
Size: 3872
MslI CAynn'nnrTG
Cuts at: 1861 1988 1861
Size: 127 3745
MspA1I CmG'CkG
Cuts at: 377 636 983 1297 1657 2670 2915 377
Size: 259 347 314 360 1013 245 1334
Fragments arranged by size:
1334 1013 360 347 314 259 245
NaeI GCC'GGC
Cuts at: 134 2053 134
Size: 1919 1953
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NcoI C'CATG_G
Cuts at: 107 857 107
Size: 750 3122
NgoMIV G'CCGG_C
Cuts at: 132 2051 132
Size: 1919 1953
NspI r_CATG'y
Cuts at: 612 925 1425 2332 612
Size: 313 500 907 2152
Fragments arranged by size:
2152 907 500 313
PasI CC'CwG_GG
Cuts at: 516 516
Size: 3872
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PfoI T'CCnGG_A
Cuts at: 713 713
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PstI C_TGCA'G
Cuts at: 919 1604 919
Size: 685 3187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 636 1297 1657 636
Size: 661 360 2851
Fragments arranged by size:
2851 661 360
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 769 1895 2105 769
Size: 1126 210 2536
Fragments arranged by size:
2536 1126 210
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SfcI C'TryA_G
Cuts at: 33 915 1600 2593 2784 33
Size: 882 685 993 191 1121
Fragments arranged by size:
1121 993 882 685 191
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 184 364 894 184
Size: 180 530 3162
Fragments arranged by size:
3162 530 180
SmlI C'TyrA_G
Cuts at: 204 1027 1942 2434 2696 204
Size: 823 915 492 262 1380
Fragments arranged by size:
1380 915 823 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
SrfI GCCC'GGGC
Cuts at: 364 364
Size: 3872
StuI AGG'CCT
Cuts at: 724 724
Size: 3872
StyI C'CwwG_G
Cuts at: 107 299 857 1006 1360 107
Size: 192 558 149 354 2619
Fragments arranged by size:
2619 558 354 192 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 3387 3387
Size: 3872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1367 1367
Size: 3872
XmaI C'CCGG_G
Cuts at: 182 362 892 182
Size: 180 530 3162
Fragments arranged by size:
3162 530 180
XmnI GAAnn'nnTTC
Cuts at: 724 767 724
Size: 43 3829
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI ZraI