Restriction map for
pF1KB8920
(Circular) (Six-base) MAPSORT of: pF1KB8920.seq Check: 1920 from: 1 to: 3914
pF1KB8920 3914 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AatII G_ACGT'C
Cuts at: 428 428
Size: 3914
AccI GT'mk_AC
Cuts at: 952 952
Size: 3914
Acc65I G'GTAC_C
Cuts at: 930 930
Size: 3914
AclI AA'CG_TT
Cuts at: 3754 3754
Size: 3914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1739 2171 2918 3186 145
Size: 1594 432 747 268 873
Fragments arranged by size:
1594 873 747 432 268
AfeI AGC'GCT
Cuts at: 517 814 517
Size: 297 3617
AflIII A'CryG_T
Cuts at: 1266 2370 3347 1266
Size: 1104 977 1833
Fragments arranged by size:
1833 1104 977
AgeI A'CCGG_T
Cuts at: 2302 2302
Size: 3914
AlwNI CAG_nnn'CTG
Cuts at: 315 1219 2786 315
Size: 904 1567 1443
Fragments arranged by size:
1567 1443 904
ApaI G_GGCC'C
Cuts at: 141 502 711 766 141
Size: 361 209 55 3289
Fragments arranged by size:
3289 361 209 55
ApaLI G'TGCA_C
Cuts at: 2684 2684
Size: 3914
ApoI r'AATT_y
Cuts at: 918 3267 3523 918
Size: 2349 256 1309
Fragments arranged by size:
2349 1309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
AvaI C'yCGr_G
Cuts at: 132 291 469 934 132
Size: 159 178 465 3112
Fragments arranged by size:
3112 465 178 159
BamHI G'GATC_C
Cuts at: 939 939
Size: 3914
BanI G'GyrC_C
Cuts at: 188 487 569 595 930 1344 1592 1627 188
Size: 299 82 26 335 414 248 35 2475
Fragments arranged by size:
2475 414 335 299 248 82 35 26
BanII G_rGCy'C
Cuts at: 141 476 502 711 766 928 1958 141
Size: 335 26 209 55 162 1030 2097
Fragments arranged by size:
2097 1030 335 209 162 55 26
BbeI G_GCGC'C
Cuts at: 573 599 1348 1596 573
Size: 26 749 248 2891
Fragments arranged by size:
2891 749 248 26
BbsI GAAGACnn'nnnn_
Cuts at: 446 446
Size: 3914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 670 704 90
Size: 34 546 34 3300
Fragments arranged by size:
3300 546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1812 2573 3914 1812
Size: 761 1341 1812
Fragments arranged by size:
1812 1341 761
BclI T'GATC_A
Cuts at: 1434 1434
Size: 3914
BglI GCCn_nnn'nGGC
Cuts at: 683 683
Size: 3914
BglII A'GATC_T
Cuts at: 1429 3466 1429
Size: 2037 1877
BlpI GC'TnA_GC
Cuts at: 1026 1026
Size: 3914
Bme1580I G_kGCm'C
Cuts at: 141 502 711 766 1539 1632 2688 141
Size: 361 209 55 773 93 1056 1367
Fragments arranged by size:
1367 1056 773 361 209 93 55
BmrI ACTGGGnnnn_n'
Cuts at: 568 1126 1207 1542 3686 568
Size: 558 81 335 2144 796
Fragments arranged by size:
2144 796 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 2281 444
Size: 1837 2077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 259 649 742 214
Size: 45 390 93 3386
Fragments arranged by size:
3386 390 93 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1969 2461 2759 1090
Size: 879 492 298 2245
Fragments arranged by size:
2245 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3618 3618
Size: 3914
BsaAI yAC'GTr
Cuts at: 1267 1267
Size: 3914
BsaBI GATnn'nnATC
Cuts at: 6 1428 3465 6
Size: 1422 2037 455
Fragments arranged by size:
2037 1422 455
BsaHI Gr'CG_yC
Cuts at: 425 570 596 850 1345 1593 2295 3791 425
Size: 145 26 254 495 248 702 1496 548
Fragments arranged by size:
1496 702 548 495 254 248 145 26
BsaWI w'CCGG_w
Cuts at: 1103 1624 2302 2576 2723 3134 1103
Size: 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 356 542 788 1339 2097 2674 3170 356
Size: 186 246 551 758 577 496 1100
Fragments arranged by size:
1100 758 577 551 496 246 186
BsiEI CG_ry'CG
Cuts at: 104 275 372 614 746 1456 1502 2710 104
Size: 171 97 242 132 710 46 1208 1308
Fragments arranged by size:
1308 1208 710 242 171 132 97 46
BsiHKAI G_wGCw'C
Cuts at: 536 728 782 928 1706 1896 2688 536
Size: 192 54 146 778 190 792 1762
Fragments arranged by size:
1762 792 778 192 190 146 54
BsmBI CGTCTCn'nnnn_
Cuts at: 432 432
Size: 3914
Bsp1286I G_dGCh'C
Cuts at: 141 476 502 536 711 728 766 782 928
Size: 335 26 34 175 17 38 16 146
Cuts at: 928 1539 1632 1706 1896 1958 2688 141
Size: 611 93 74 190 62 730 1367
Fragments arranged by size:
1367 730 611 335 190 175 146 93
74 62 38 34 26 17 16
BspEI T'CCGG_A
Cuts at: 3134 3134
Size: 3914
BspHI T'CATG_A
Cuts at: 827 3090 827
Size: 2263 1651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 964 1178 1480 1861 242
Size: 722 214 302 381 2295
Fragments arranged by size:
2295 722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 2206 2260 3744 3909 50
Size: 81 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 1826
Size: 3914
BsrFI r'CCGG_y
Cuts at: 552 1912 2093 2302 552
Size: 1360 181 209 2164
Fragments arranged by size:
2164 1360 209 181
BssSI C'ACGA_G
Cuts at: 2185 2543 2185
Size: 358 3556
BstAPI GCAn_nnn'nTGC
Cuts at: 732 1291 732
Size: 559 3355
BstBI TT'CG_AA
Cuts at: 2275 2275
Size: 3914
BstYI r'GATC_y
Cuts at: 1 939 1406 1429 1764 2010 3022 3108 3120
Size: 938 467 23 335 246 1012 86 12
Cuts at: 3120 3466 1
Size: 346 449
Fragments arranged by size:
1012 938 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 458 458
Size: 3914
BtgI C'CryG_G
Cuts at: 170 221 527 170
Size: 51 306 3557
Fragments arranged by size:
3557 306 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 497 1864 2045 2208 497
Size: 1367 181 163 2203
Fragments arranged by size:
2203 1367 181 163
BtsI GCAGTG_nn'
Cuts at: 1157 1157
Size: 3914
DraI TTT'AAA
Cuts at: 913 913
Size: 3914
DrdI GACnn_nn'nnGTC
Cuts at: 1620 2478 1620
Size: 858 3056
EaeI y'GGCC_r
Cuts at: 272 369 611 651 743 1499 1673 2064 2091
Size: 97 242 40 92 756 174 391 27
Cuts at: 2091 3436 272
Size: 1345 750
Fragments arranged by size:
1345 756 750 391 242 174 97 92
40 27
EagI C'GGCC_G
Cuts at: 272 369 611 743 1499 272
Size: 97 242 132 756 2687
Fragments arranged by size:
2687 756 242 132 97
EarI CTCTTCn'nnn_
Cuts at: 835 1937 2147 835
Size: 1102 210 2602
Fragments arranged by size:
2602 1102 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2122 2432 2578 3724 2122
Size: 310 146 1146 2312
Fragments arranged by size:
2312 1146 310 146
EcoICRI GAG'CTC
Cuts at: 926 926
Size: 3914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 214 259 649 742 1739 2171 2918 3186
Size: 69 45 390 93 997 432 747 268
Cuts at: 3186 145
Size: 873
Fragments arranged by size:
997 873 747 432 390 268 93 69
45
EcoO109I rG'GnC_Cy
Cuts at: 137 138 213 707 762 763 1053 137
Size: 1 75 494 55 1 290 2998
Fragments arranged by size:
2998 494 290 75 55 1 1
EcoRI G'AATT_C
Cuts at: 918 918
Size: 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3914
FspI TGC'GCA
Cuts at: 1695 1695
Size: 3914
HaeII r_GCGC'y
Cuts at: 519 573 599 816 1348 1596 2618 3597 519
Size: 54 26 217 532 248 1022 979 836
Fragments arranged by size:
1022 979 836 532 248 217 54 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 592 624 1756 1786 1788 1818 592
Size: 32 1132 30 2 30 2688
Fragments arranged by size:
2688 1132 32 30 30 2
HincII GTy'rAC
Cuts at: 953 953
Size: 3914
KasI G'GCGC_C
Cuts at: 569 595 1344 1592 569
Size: 26 749 248 2891
Fragments arranged by size:
2891 749 248 26
KpnI G_GTAC'C
Cuts at: 934 934
Size: 3914
MluI A'CGCG_T
Cuts at: 3347 3347
Size: 3914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2769 2585
Size: 184 3730
MscI TGG'CCA
Cuts at: 653 1675 653
Size: 1022 2892
MslI CAynn'nnrTG
Cuts at: 175 1903 2030 175
Size: 1728 127 2059
Fragments arranged by size:
2059 1728 127
MspA1I CmG'CkG
Cuts at: 356 392 401 433 453 542 788 1025 1339
Size: 36 9 32 20 89 246 237 314
Cuts at: 1339 1699 2712 2957 356
Size: 360 1013 245 1313
Fragments arranged by size:
1313 1013 360 314 246 245 237 89
36 32 20 9
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 3914
NarI GG'CG_CC
Cuts at: 570 596 1345 1593 570
Size: 26 749 248 2891
Fragments arranged by size:
2891 749 248 26
NcoI C'CATG_G
Cuts at: 170 527 170
Size: 357 3557
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 3914
NspI r_CATG'y
Cuts at: 967 1467 2374 967
Size: 500 907 2507
Fragments arranged by size:
2507 907 500
PciI A'CATG_T
Cuts at: 2370 2370
Size: 3914
PfoI T'CCnGG_A
Cuts at: 662 662
Size: 3914
PmeI GTTT'AAAC
Cuts at: 913 913
Size: 3914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 3882
PpuMI rG'GwC_Cy
Cuts at: 213 213
Size: 3914
PspOMI G'GGCC_C
Cuts at: 137 498 707 762 137
Size: 361 209 55 3289
Fragments arranged by size:
3289 361 209 55
PstI C_TGCA'G
Cuts at: 127 961 1646 127
Size: 834 685 2395
Fragments arranged by size:
2395 834 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3914
PvuII CAG'CTG
Cuts at: 1339 1699 1339
Size: 360 3554
RsrII CG'GwC_CG
Cuts at: 2109 2109
Size: 3914
SacI G_AGCT'C
Cuts at: 928 928
Size: 3914
SalI G'TCGA_C
Cuts at: 951 951
Size: 3914
SapI GCTCTTCn'nnn_
Cuts at: 835 1937 2147 835
Size: 1102 210 2602
Fragments arranged by size:
2602 1102 210
SbfI CC_TGCA'GG
Cuts at: 961 961
Size: 3914
ScaI AGT'ACT
Cuts at: 3431 3431
Size: 3914
SfcI C'TryA_G
Cuts at: 33 123 768 957 1642 2635 2826 33
Size: 90 645 189 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 191 189 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 683 683
Size: 3914
SfoI GGC'GCC
Cuts at: 571 597 1346 1594 571
Size: 26 749 248 2891
Fragments arranged by size:
2891 749 248 26
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
SmaI CCC'GGG
Cuts at: 293 936 293
Size: 643 3271
SmlI C'TyrA_G
Cuts at: 1069 1984 2476 2738 1069
Size: 915 492 262 2245
Fragments arranged by size:
2245 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3914
SphI G_CATG'C
Cuts at: 967 1467 967
Size: 500 3414
StuI AGG'CCT
Cuts at: 166 619 166
Size: 453 3461
StyI C'CwwG_G
Cuts at: 170 527 803 1048 1402 170
Size: 357 276 245 354 2682
Fragments arranged by size:
2682 357 354 276 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 377 1497 1630 2089 2298 334
Size: 43 1120 133 459 209 1950
Fragments arranged by size:
1950 1120 459 209 133 43
TatI w'GTAC_w
Cuts at: 3429 3429
Size: 3914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 3882
Tth111I GACn'n_nGTC
Cuts at: 1711 1711
Size: 3914
XbaI T'CTAG_A
Cuts at: 945 945
Size: 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1409 1409
Size: 3914
XmaI C'CCGG_G
Cuts at: 291 934 291
Size: 643 3271
ZraI GAC'GTC
Cuts at: 426 426
Size: 3914
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI