Restriction map for
pF1KB8922
(Circular) (Six-base) MAPSORT of: pF1KB8922.seq Check: 3394 from: 1 to: 3920
pF1KB8922 3920 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 709 958 709
Size: 249 3671
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1745 2177 2924 3192 345
Size: 1400 432 747 268 1073
Fragments arranged by size:
1400 1073 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 727 759 727
Size: 32 3888
AlwNI CAG_nnn'CTG
Cuts at: 726 1225 2792 726
Size: 499 1567 1854
Fragments arranged by size:
1854 1567 499
ApaI G_GGCC'C
Cuts at: 699 699
Size: 3920
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 135 397 572 613 940 135
Size: 262 175 41 327 3115
Fragments arranged by size:
3115 327 262 175 41
BamHI G'GATC_C
Cuts at: 213 945 213
Size: 732 3188
BanI G'GyrC_C
Cuts at: 433 936 1350 1598 1633 433
Size: 503 414 248 35 2720
Fragments arranged by size:
2720 503 414 248 35
BanII G_rGCy'C
Cuts at: 115 325 356 590 699 934 1964 115
Size: 210 31 234 109 235 1030 2071
Fragments arranged by size:
2071 1030 235 234 210 109 31
BbeI G_GCGC'C
Cuts at: 437 1354 1602 437
Size: 917 248 2755
Fragments arranged by size:
2755 917 248
BbvCI CC'TCA_GC
Cuts at: 268 474 268
Size: 206 3714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 768 802 768
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglI GCCn_nnn'nGGC
Cuts at: 618 618
Size: 3920
BglII A'GATC_T
Cuts at: 824 1435 3472 824
Size: 611 2037 1272
Fragments arranged by size:
2037 1272 611
BlpI GC'TnA_GC
Cuts at: 553 1032 553
Size: 479 3441
Bme1580I G_kGCm'C
Cuts at: 699 1545 1638 2694 699
Size: 846 93 1056 1925
Fragments arranged by size:
1925 1056 846 93
BmrI ACTGGGnnnn_n'
Cuts at: 1132 1213 1548 3692 1132
Size: 81 335 2144 1360
Fragments arranged by size:
2144 1360 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 468 748 279
Size: 189 280 3451
Fragments arranged by size:
3451 280 189
Bpu10I CC'TnA_GC
Cuts at: 268 474 268
Size: 206 3714
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 823 1434 3471 6
Size: 817 611 2037 455
Fragments arranged by size:
2037 817 611 455
BsaHI Gr'CG_yC
Cuts at: 434 1351 1599 2301 3797 434
Size: 917 248 702 1496 557
Fragments arranged by size:
1496 917 702 557 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 273 282 273
Size: 9 3911
BseYI C'CCAG_C
Cuts at: 562 1345 2103 2680 3176 562
Size: 783 758 577 496 1306
Fragments arranged by size:
1306 783 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 396
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 325 934 1712 1902 2694 115
Size: 210 609 778 190 792 1341
Fragments arranged by size:
1341 792 778 609 210 190
Bsp1286I G_dGCh'C
Cuts at: 115 325 356 590 699 934 1545 1638 1712
Size: 210 31 234 109 235 611 93 74
Cuts at: 1712 1902 1964 2694 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 235 234 210 190 109
93 74 62 31
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 970 1184 1486 1867 970
Size: 214 302 381 3023
Fragments arranged by size:
3023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 200 281 2212 2266 3750 3915 50
Size: 150 81 1931 54 1484 165 55
Fragments arranged by size:
1931 1484 165 150 81 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 232 839 1918 2099 2308 232
Size: 607 1079 181 209 1844
Fragments arranged by size:
1844 1079 607 209 181
BssHII G'CGCG_C
Cuts at: 327 327
Size: 3920
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 213 824 945 1412 1435 1770 2016 3028
Size: 212 611 121 467 23 335 246 1012
Cuts at: 3028 3114 3126 3472 1
Size: 86 12 346 449
Fragments arranged by size:
1012 611 467 449 346 335 246 212
121 86 23 12
BstZ17I GTA'TAC
Cuts at: 710 710
Size: 3920
Bsu36I CC'TnA_GG
Cuts at: 533 533
Size: 3920
BtgI C'CryG_G
Cuts at: 429 590 429
Size: 161 3759
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 189 1163 189
Size: 974 2946
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 271 1626 2484 271
Size: 1355 858 1707
Fragments arranged by size:
1707 1355 858
EaeI y'GGCC_r
Cuts at: 593 1505 1679 2070 2097 3442 593
Size: 912 174 391 27 1345 1071
Fragments arranged by size:
1345 1071 912 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 323 932 113
Size: 210 609 3101
Fragments arranged by size:
3101 609 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 345 468 748 1745 2177 2924 3192 279
Size: 66 123 280 997 432 747 268 1007
Fragments arranged by size:
1007 997 747 432 280 268 123 66
EcoNI CCTnn'n_nnAGG
Cuts at: 645 645
Size: 3920
EcoO109I rG'GnC_Cy
Cuts at: 695 1059 695
Size: 364 3556
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 437 491 816 905 1354 1602 2624 3603 437
Size: 54 325 89 449 248 1022 979 754
Fragments arranged by size:
1022 979 754 449 325 248 89 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 764 796 1762 1792 1794 1824 764
Size: 32 966 30 2 30 2860
Fragments arranged by size:
2860 966 32 30 30 2
HincII GTy'rAC
Cuts at: 276 959 276
Size: 683 3237
HindIII A'AGCT_T
Cuts at: 652 652
Size: 3920
KasI G'GCGC_C
Cuts at: 433 1350 1598 433
Size: 917 248 2755
Fragments arranged by size:
2755 917 248
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 2591 2775 485
Size: 2106 184 1630
Fragments arranged by size:
2106 1630 184
MscI TGG'CCA
Cuts at: 595 1681 595
Size: 1086 2834
MslI CAynn'nnrTG
Cuts at: 1909 2036 1909
Size: 127 3793
MspA1I CmG'CkG
Cuts at: 220 895 1031 1345 1705 2718 2963 220
Size: 675 136 314 360 1013 245 1177
Fragments arranged by size:
1177 1013 675 360 314 245 136
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 434 1351 1599 434
Size: 917 248 2755
Fragments arranged by size:
2755 917 248
NcoI C'CATG_G
Cuts at: 590 590
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PflMI CCAn_nnn'nTGG
Cuts at: 726 726
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PspOMI G'GGCC_C
Cuts at: 695 695
Size: 3920
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 895 1345 1705 895
Size: 450 360 3110
Fragments arranged by size:
3110 450 360
RsrII CG'GwC_CG
Cuts at: 701 2115 701
Size: 1414 2506
SacI G_AGCT'C
Cuts at: 115 325 934 115
Size: 210 609 3101
Fragments arranged by size:
3101 609 210
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 890 963 1648 2641 2832 33
Size: 857 73 685 993 191 1121
Fragments arranged by size:
1121 993 857 685 191 73
SfoI GGC'GCC
Cuts at: 435 1352 1600 435
Size: 917 248 2755
Fragments arranged by size:
2755 917 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 590 1054 1408 590
Size: 464 354 3102
Fragments arranged by size:
3102 464 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstXI ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI