Restriction map for
pF1KB8932
(Circular) (Six-base) MAPSORT of: pF1KB8932.seq Check: 9746 from: 1 to: 3962
pF1KB8932 3962 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 478 1000 478
Size: 522 3440
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3962
AclI AA'CG_TT
Cuts at: 3802 3802
Size: 3962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1787 2219 2966 3234 1787
Size: 432 747 268 2515
Fragments arranged by size:
2515 747 432 268
AflIII A'CryG_T
Cuts at: 491 1314 2418 3395 491
Size: 823 1104 977 1058
Fragments arranged by size:
1104 1058 977 823
AgeI A'CCGG_T
Cuts at: 2350 2350
Size: 3962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 583 615 583
Size: 32 3930
AlwNI CAG_nnn'CTG
Cuts at: 1267 2834 1267
Size: 1567 2395
ApaI G_GGCC'C
Cuts at: 404 404
Size: 3962
ApaLI G'TGCA_C
Cuts at: 2732 2732
Size: 3962
ApoI r'AATT_y
Cuts at: 966 3315 3571 966
Size: 2349 256 1357
Fragments arranged by size:
2349 1357 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 875 982 875
Size: 107 3855
BamHI G'GATC_C
Cuts at: 987 987
Size: 3962
BanI G'GyrC_C
Cuts at: 180 262 315 517 978 1392 1640 1675 180
Size: 82 53 202 461 414 248 35 2467
Fragments arranged by size:
2467 461 414 248 202 82 53 35
BanII G_rGCy'C
Cuts at: 404 443 976 2006 404
Size: 39 533 1030 2360
Fragments arranged by size:
2360 1030 533 39
BbeI G_GCGC'C
Cuts at: 184 266 521 1396 1644 184
Size: 82 255 875 248 2502
Fragments arranged by size:
2502 875 255 248 82
BciVI GTATCCnnnnn_n'
Cuts at: 1860 2621 3962 1860
Size: 761 1341 1860
Fragments arranged by size:
1860 1341 761
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3962
BglI GCCn_nnn'nGGC
Cuts at: 399 939 399
Size: 540 3422
BglII A'GATC_T
Cuts at: 680 1477 3514 680
Size: 797 2037 1128
Fragments arranged by size:
2037 1128 797
BlpI GC'TnA_GC
Cuts at: 371 1074 371
Size: 703 3259
Bme1580I G_kGCm'C
Cuts at: 320 404 1587 1680 2736 320
Size: 84 1183 93 1056 1546
Fragments arranged by size:
1546 1183 1056 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 1174 1255 1590 3734 1174
Size: 81 335 2144 1402
Fragments arranged by size:
2144 1402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 3962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 454 610 906 298
Size: 156 156 296 3354
Fragments arranged by size:
3354 296 156 156
Bpu10I CC'TnA_GC
Cuts at: 776 901 776
Size: 125 3837
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2017 2509 2807 1138
Size: 879 492 298 2293
Fragments arranged by size:
2293 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3666 3666
Size: 3962
BsaAI yAC'GTr
Cuts at: 1315 1315
Size: 3962
BsaBI GATnn'nnATC
Cuts at: 6 1476 3513 6
Size: 1470 2037 455
Fragments arranged by size:
2037 1470 455
BsaHI Gr'CG_yC
Cuts at: 181 263 428 518 1393 1641 2343 3839 181
Size: 82 165 90 875 248 702 1496 304
Fragments arranged by size:
1496 875 702 304 248 165 90 82
BsaWI w'CCGG_w
Cuts at: 1151 1672 2350 2624 2771 3182 1151
Size: 521 678 274 147 411 1931
Fragments arranged by size:
1931 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 583 613 833 863 583
Size: 30 220 30 3682
Fragments arranged by size:
3682 220 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 451 553 733 820 133
Size: 318 102 180 87 3275
Fragments arranged by size:
3275 318 180 102 87
BseYI C'CCAG_C
Cuts at: 1387 2145 2722 3218 1387
Size: 758 577 496 2131
Fragments arranged by size:
2131 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 628 943 1504 1550 2758 104
Size: 524 315 561 46 1208 1308
Fragments arranged by size:
1308 1208 561 524 315 46
BsiHKAI G_wGCw'C
Cuts at: 976 1754 1944 2736 976
Size: 778 190 792 2202
Fragments arranged by size:
2202 792 778 190
BsiWI C'GTAC_G
Cuts at: 308 308
Size: 3962
Bsp1286I G_dGCh'C
Cuts at: 320 404 443 976 1587 1680 1754 1944 2006
Size: 84 39 533 611 93 74 190 62
Cuts at: 2006 2736 320
Size: 730 1546
Fragments arranged by size:
1546 730 611 533 190 93 84 74
62 39
BspEI T'CCGG_A
Cuts at: 3182 3182
Size: 3962
BspHI T'CATG_A
Cuts at: 3138 3138
Size: 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1012 1226 1528 1909 1012
Size: 214 302 381 3065
Fragments arranged by size:
3065 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2254 2308 3792 3957 50
Size: 2204 54 1484 165 55
Fragments arranged by size:
2204 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 1874
Size: 3962
BsrFI r'CCGG_y
Cuts at: 1960 2141 2350 1960
Size: 181 209 3572
Fragments arranged by size:
3572 209 181
BsrGI T'GTAC_A
Cuts at: 215 215
Size: 3962
BssHII G'CGCG_C
Cuts at: 272 570 272
Size: 298 3664
BssSI C'ACGA_G
Cuts at: 953 2233 2591 953
Size: 1280 358 2324
Fragments arranged by size:
2324 1280 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1339 1339
Size: 3962
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstYI r'GATC_y
Cuts at: 1 680 987 1454 1477 1812 2058 3070 3156
Size: 679 307 467 23 335 246 1012 86
Cuts at: 3156 3168 3514 1
Size: 12 346 449
Fragments arranged by size:
1012 679 467 449 346 335 307 246
86 23 12
BtgI C'CryG_G
Cuts at: 338 466 338
Size: 128 3834
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1912 2093 2256 1912
Size: 181 163 3618
Fragments arranged by size:
3618 181 163
BtsI GCAGTG_nn'
Cuts at: 1205 1205
Size: 3962
DraI TTT'AAA
Cuts at: 961 961
Size: 3962
DrdI GACnn_nn'nnGTC
Cuts at: 1668 2526 1668
Size: 858 3104
EaeI y'GGCC_r
Cuts at: 134 625 940 1547 1721 2112 2139 3484 134
Size: 491 315 607 174 391 27 1345 612
Fragments arranged by size:
1345 612 607 491 391 315 174 27
EagI C'GGCC_G
Cuts at: 625 940 1547 625
Size: 315 607 3040
Fragments arranged by size:
3040 607 315
EarI CTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2170 2480 2626 3772 2170
Size: 310 146 1146 2360
Fragments arranged by size:
2360 1146 310 146
EcoICRI GAG'CTC
Cuts at: 974 974
Size: 3962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 454 610 906 1787 2219 2966 3234 298
Size: 156 156 296 881 432 747 268 1026
Fragments arranged by size:
1026 881 747 432 296 268 156 156
EcoO109I rG'GnC_Cy
Cuts at: 153 401 1101 153
Size: 248 700 3014
Fragments arranged by size:
3014 700 248
EcoRI G'AATT_C
Cuts at: 966 966
Size: 3962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 1743 1743
Size: 3962
HaeII r_GCGC'y
Cuts at: 184 197 266 521 820 1396 1644 2666 3645
Size: 13 69 255 299 576 248 1022 979
Cuts at: 3645 184
Size: 501
Fragments arranged by size:
1022 979 576 501 299 255 248 69
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1804 1834 1836 1866 1804
Size: 30 2 30 3900
Fragments arranged by size:
3900 30 30 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3962
KasI G'GCGC_C
Cuts at: 180 262 517 1392 1640 180
Size: 82 255 875 248 2502
Fragments arranged by size:
2502 875 255 248 82
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3962
MluI A'CGCG_T
Cuts at: 491 3395 491
Size: 2904 1058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2817 2633
Size: 184 3778
MscI TGG'CCA
Cuts at: 1723 1723
Size: 3962
MslI CAynn'nnrTG
Cuts at: 1951 2078 1951
Size: 127 3835
MspA1I CmG'CkG
Cuts at: 340 456 579 748 859 1073 1387 1747 2760
Size: 116 123 169 111 214 314 360 1013
Cuts at: 2760 3005 340
Size: 245 1297
Fragments arranged by size:
1297 1013 360 314 245 214 169 123
116 111
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 181 263 518 1393 1641 181
Size: 82 255 875 248 2502
Fragments arranged by size:
2502 875 255 248 82
NcoI C'CATG_G
Cuts at: 466 466
Size: 3962
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NotI GC'GGCC_GC
Cuts at: 940 940
Size: 3962
NspI r_CATG'y
Cuts at: 1015 1515 2422 1015
Size: 500 907 2555
Fragments arranged by size:
2555 907 500
PasI CC'CwG_GG
Cuts at: 267 267
Size: 3962
PciI A'CATG_T
Cuts at: 2418 2418
Size: 3962
PfoI T'CCnGG_A
Cuts at: 407 431 407
Size: 24 3938
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 615 3101 3133 583
Size: 32 2486 32 1412
Fragments arranged by size:
2486 1412 32 32
PpuMI rG'GwC_Cy
Cuts at: 153 153
Size: 3962
PshAI GACnn'nnGTC
Cuts at: 748 748
Size: 3962
PspOMI G'GGCC_C
Cuts at: 400 400
Size: 3962
PstI C_TGCA'G
Cuts at: 1009 1694 1009
Size: 685 3277
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 456 579 748 1387 1747 456
Size: 123 169 639 360 2671
Fragments arranged by size:
2671 639 360 169 123
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 976 976
Size: 3962
SacII CC_GC'GG
Cuts at: 341 341
Size: 3962
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 127 3479 127
Size: 3352 610
SfcI C'TryA_G
Cuts at: 33 1005 1690 2683 2874 33
Size: 972 685 993 191 1121
Fragments arranged by size:
1121 993 972 685 191
SfoI GGC'GCC
Cuts at: 182 264 519 1394 1642 182
Size: 82 255 875 248 2502
Fragments arranged by size:
2502 875 255 248 82
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SmaI CCC'GGG
Cuts at: 984 984
Size: 3962
SmlI C'TyrA_G
Cuts at: 1117 2032 2524 2786 1117
Size: 915 492 262 2293
Fragments arranged by size:
2293 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3462
StyI C'CwwG_G
Cuts at: 466 1096 1450 466
Size: 630 354 2978
Fragments arranged by size:
2978 630 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 679 1545 1678 2137 2346 679
Size: 866 133 459 209 2295
Fragments arranged by size:
2295 866 459 209 133
TatI w'GTAC_w
Cuts at: 125 215 614 3477 125
Size: 90 399 2863 610
Fragments arranged by size:
2863 610 399 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 833 865 3479 3511 833
Size: 32 2614 32 1284
Fragments arranged by size:
2614 1284 32 32
Tth111I GACn'n_nGTC
Cuts at: 1759 1759
Size: 3962
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1457 1457
Size: 3962
XmaI C'CCGG_G
Cuts at: 982 982
Size: 3962
XmnI GAAnn'nnTTC
Cuts at: 598 598
Size: 3962
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsmI BsmBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PflMI PmlI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI