Restriction map for
pF1KB8939
(Circular) (Six-base) MAPSORT of: pF1KB8939.seq Check: 372 from: 1 to: 3986
pF1KB8939 3986 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1024 1024
Size: 3986
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1811 2243 2990 3258 1811
Size: 432 747 268 2539
Fragments arranged by size:
2539 747 432 268
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 363 494 617 1291 2858 363
Size: 131 123 674 1567 1491
Fragments arranged by size:
1567 1491 674 131 123
ApaI G_GGCC'C
Cuts at: 862 862
Size: 3986
ApaLI G'TGCA_C
Cuts at: 2756 2756
Size: 3986
ApoI r'AATT_y
Cuts at: 448 990 3339 3595 448
Size: 542 2349 256 839
Fragments arranged by size:
2349 839 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 1006 1006
Size: 3986
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 418 882 1002 1416 1664 1699 418
Size: 464 120 414 248 35 2705
Fragments arranged by size:
2705 464 414 248 120 35
BanII G_rGCy'C
Cuts at: 305 862 979 1000 2030 305
Size: 557 117 21 1030 2261
Fragments arranged by size:
2261 1030 557 117 21
BbeI G_GCGC'C
Cuts at: 422 1420 1668 422
Size: 998 248 2740
Fragments arranged by size:
2740 998 248
BbsI GAAGACnn'nnnn_
Cuts at: 760 760
Size: 3986
BbvCI CC'TCA_GC
Cuts at: 805 805
Size: 3986
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 1506 1506
Size: 3986
BglI GCCn_nnn'nGGC
Cuts at: 274 822 274
Size: 548 3438
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 1098 1098
Size: 3986
Bme1580I G_kGCm'C
Cuts at: 862 1611 1704 2760 862
Size: 749 93 1056 2088
Fragments arranged by size:
2088 1056 749 93
BmrI ACTGGGnnnn_n'
Cuts at: 1198 1279 1614 3758 1198
Size: 81 335 2144 1426
Fragments arranged by size:
2144 1426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 3986
Bpu10I CC'TnA_GC
Cuts at: 805 805
Size: 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 355 1162 2041 2533 2831 241
Size: 114 807 879 492 298 1396
Fragments arranged by size:
1396 879 807 492 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 478 3690 478
Size: 3212 774
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 419 769 1417 1665 2367 3863 419
Size: 350 648 248 702 1496 542
Fragments arranged by size:
1496 702 648 542 350 248
BsaWI w'CCGG_w
Cuts at: 1175 1696 2374 2648 2795 3206 1175
Size: 521 678 274 147 411 1955
Fragments arranged by size:
1955 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 667 697 961 991 667
Size: 30 264 30 3662
Fragments arranged by size:
3662 264 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 793 496
Size: 297 3689
BseYI C'CCAG_C
Cuts at: 195 862 1411 2169 2746 3242 195
Size: 667 549 758 577 496 939
Fragments arranged by size:
939 758 667 577 549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 820
Size: 3986
BsiEI CG_ry'CG
Cuts at: 104 408 645 653 1528 1574 2782 104
Size: 304 237 8 875 46 1208 1308
Fragments arranged by size:
1308 1208 875 304 237 46 8
BsiHKAI G_wGCw'C
Cuts at: 233 257 305 1000 1778 1968 2760 233
Size: 24 48 695 778 190 792 1459
Fragments arranged by size:
1459 792 778 695 190 48 24
Bsp1286I G_dGCh'C
Cuts at: 233 257 305 862 979 1000 1611 1704 1778
Size: 24 48 557 117 21 611 93 74
Cuts at: 1778 1968 2030 2760 233
Size: 190 62 730 1459
Fragments arranged by size:
1459 730 611 557 190 117 93 74
62 48 24 21
BspEI T'CCGG_A
Cuts at: 3206 3206
Size: 3986
BspHI T'CATG_A
Cuts at: 3162 3162
Size: 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1250 1552 1933 1036
Size: 214 302 381 3089
Fragments arranged by size:
3089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 192 901 2278 2332 3816 3981 50
Size: 142 709 1377 54 1484 165 55
Fragments arranged by size:
1484 1377 709 165 142 55 54
BsrDI GCAATG_nn'
Cuts at: 1898 1898
Size: 3986
BsrFI r'CCGG_y
Cuts at: 629 879 1984 2165 2374 629
Size: 250 1105 181 209 2241
Fragments arranged by size:
2241 1105 250 209 181
BssSI C'ACGA_G
Cuts at: 964 2257 2615 964
Size: 1293 358 2335
Fragments arranged by size:
2335 1293 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 1363
Size: 3986
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 270 966 270
Size: 696 3290
BstYI r'GATC_y
Cuts at: 1 311 1011 1478 1501 1836 2082 3094 3180
Size: 310 700 467 23 335 246 1012 86
Cuts at: 3180 3192 3538 1
Size: 12 346 449
Fragments arranged by size:
1012 700 467 449 346 335 310 246
86 23 12
BtgI C'CryG_G
Cuts at: 269 285 422 587 921 269
Size: 16 137 165 334 3334
Fragments arranged by size:
3334 334 165 137 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 796 875 907 1229 796
Size: 79 32 322 3553
Fragments arranged by size:
3553 322 79 32
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1692 2550 1692
Size: 858 3128
EaeI y'GGCC_r
Cuts at: 405 650 1571 1745 2136 2163 3508 405
Size: 245 921 174 391 27 1345 883
Fragments arranged by size:
1345 921 883 391 245 174 27
EagI C'GGCC_G
Cuts at: 405 650 1571 405
Size: 245 921 2820
Fragments arranged by size:
2820 921 245
EarI CTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 406 595 905 2194 2504 2650 3796 406
Size: 189 310 1289 310 146 1146 596
Fragments arranged by size:
1289 1146 596 310 310 189 146
EcoICRI GAG'CTC
Cuts at: 303 998 303
Size: 695 3291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1811 2243 2990 3258 337
Size: 1474 432 747 268 1065
Fragments arranged by size:
1474 1065 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 859 1125 859
Size: 266 3720
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 422 1420 1668 2690 3669 422
Size: 998 248 1022 979 739
Fragments arranged by size:
1022 998 979 739 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 961 993 1828 1858 1860 1890 766
Size: 32 163 32 835 30 2 30 2862
Fragments arranged by size:
2862 835 163 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1025 1025
Size: 3986
KasI G'GCGC_C
Cuts at: 418 1416 1664 418
Size: 998 248 2740
Fragments arranged by size:
2740 998 248
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2841 2657
Size: 184 3802
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 112 242 920 1975 2102 112
Size: 130 678 1055 127 1996
Fragments arranged by size:
1996 1055 678 130 127
MspA1I CmG'CkG
Cuts at: 287 363 456 708 719 1097 1411 1771 2784
Size: 76 93 252 11 378 314 360 1013
Cuts at: 2784 3029 287
Size: 245 1244
Fragments arranged by size:
1244 1013 378 360 314 252 245 93
76 11
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 3986
NarI GG'CG_CC
Cuts at: 419 1417 1665 419
Size: 998 248 2740
Fragments arranged by size:
2740 998 248
NcoI C'CATG_G
Cuts at: 921 921
Size: 3986
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 3986
NspI r_CATG'y
Cuts at: 1039 1539 2446 1039
Size: 500 907 2579
Fragments arranged by size:
2579 907 500
PasI CC'CwG_GG
Cuts at: 414 820 414
Size: 406 3580
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PfoI T'CCnGG_A
Cuts at: 314 314
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PspOMI G'GGCC_C
Cuts at: 858 858
Size: 3986
PstI C_TGCA'G
Cuts at: 173 359 932 1033 1718 173
Size: 186 573 101 685 2441
Fragments arranged by size:
2441 685 573 186 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 363 1411 1771 363
Size: 1048 360 2578
Fragments arranged by size:
2578 1048 360
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 305 1000 305
Size: 695 3291
SacII CC_GC'GG
Cuts at: 288 288
Size: 3986
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 932 1033 932
Size: 101 3885
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SfcI C'TryA_G
Cuts at: 33 169 355 928 1029 1714 2707 2898 33
Size: 136 186 573 101 685 993 191 1121
Fragments arranged by size:
1121 993 685 573 191 186 136 101
SfiI GGCCn_nnn'nGGCC
Cuts at: 274 274
Size: 3986
SfoI GGC'GCC
Cuts at: 420 1418 1666 420
Size: 998 248 2740
Fragments arranged by size:
2740 998 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 256 370 1141 2056 2548 2810 256
Size: 114 771 915 492 262 1432
Fragments arranged by size:
1432 915 771 492 262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 1039 1539 1039
Size: 500 3486
StyI C'CwwG_G
Cuts at: 263 659 921 1120 1474 263
Size: 396 262 199 354 2775
Fragments arranged by size:
2775 396 354 262 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1569 1702 2161 2370 1569
Size: 133 459 209 3185
Fragments arranged by size:
3185 459 209 133
TatI w'GTAC_w
Cuts at: 3501 3501
Size: 3986
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 769 801 3503 3535 769
Size: 32 2702 32 1220
Fragments arranged by size:
2702 1220 32 32
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 1481 270
Size: 1211 2775
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI