Restriction map for
pF1KB8952
(Circular) (Six-base) MAPSORT of: pF1KB8952.seq Check: 5837 from: 1 to: 4094
pF1KB8952 4094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 714 1132 714
Size: 418 3676
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 941 1919 2351 3098 3366 941
Size: 978 432 747 268 1669
Fragments arranged by size:
1669 978 747 432 268
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 1399 2966 1399
Size: 1567 2527
ApaI G_GGCC'C
Cuts at: 201 535 201
Size: 334 3760
ApaLI G'TGCA_C
Cuts at: 2864 2864
Size: 4094
ApoI r'AATT_y
Cuts at: 839 888 1026 1098 3447 3703 839
Size: 49 138 72 2349 256 1230
Fragments arranged by size:
2349 1230 256 138 72 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 182 251 377 507 528 552 1114 182
Size: 69 126 130 21 24 562 3162
Fragments arranged by size:
3162 562 130 126 69 24 21
AvrII C'CTAG_G
Cuts at: 288 288
Size: 4094
BamHI G'GATC_C
Cuts at: 239 1119 239
Size: 880 3214
BanI G'GyrC_C
Cuts at: 1110 1524 1772 1807 1110
Size: 414 248 35 3397
Fragments arranged by size:
3397 414 248 35
BanII G_rGCy'C
Cuts at: 201 496 535 1108 2138 201
Size: 295 39 573 1030 2157
Fragments arranged by size:
2157 1030 573 295 39
BbeI G_GCGC'C
Cuts at: 1528 1776 1528
Size: 248 3846
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglI GCCn_nnn'nGGC
Cuts at: 547 547
Size: 4094
BglII A'GATC_T
Cuts at: 1609 3646 1609
Size: 2037 2057
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 201 258 535 1719 1812 2868 201
Size: 57 277 1184 93 1056 1427
Fragments arranged by size:
1427 1184 1056 277 93 57
BmrI ACTGGGnnnn_n'
Cuts at: 1306 1387 1722 3866 1306
Size: 81 335 2144 1534
Fragments arranged by size:
2144 1534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 499 314
Size: 185 3909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3798 3798
Size: 4094
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 1525 1773 2475 3971 1525
Size: 248 702 1496 1648
Fragments arranged by size:
1648 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1283 1804 2482 2756 2903 3314 1283
Size: 521 678 274 147 411 2063
Fragments arranged by size:
2063 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 204 234 204
Size: 30 4064
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 365 1063 365
Size: 698 3396
BseYI C'CCAG_C
Cuts at: 317 439 946 1519 2277 2854 3350 317
Size: 122 507 573 758 577 496 1061
Fragments arranged by size:
1061 758 577 573 507 496 122
BsiEI CG_ry'CG
Cuts at: 104 472 489 503 539 1636 1682 2890 104
Size: 368 17 14 36 1097 46 1208 1308
Fragments arranged by size:
1308 1208 1097 368 46 36 17 14
BsiHKAI G_wGCw'C
Cuts at: 1108 1886 2076 2868 1108
Size: 778 190 792 2334
Fragments arranged by size:
2334 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 123 123
Size: 4094
Bsp1286I G_dGCh'C
Cuts at: 201 258 496 535 1108 1719 1812 1886 2076
Size: 57 238 39 573 611 93 74 190
Cuts at: 2076 2138 2868 201
Size: 62 730 1427
Fragments arranged by size:
1427 730 611 573 238 190 93 74
62 57 39
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 4094
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1144 1358 1660 2041 1144
Size: 214 302 381 3197
Fragments arranged by size:
3197 381 302 214
BsrBI CCG'CTC
Cuts at: 50 141 512 2386 2440 3924 4089 50
Size: 91 371 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 371 165 91 55 54
BsrDI GCAATG_nn'
Cuts at: 2006 2006
Size: 4094
BsrFI r'CCGG_y
Cuts at: 467 502 571 2092 2273 2482 467
Size: 35 69 1521 181 209 2079
Fragments arranged by size:
2079 1521 209 181 69 35
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 1471 1471
Size: 4094
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstEII G'GTnAC_C
Cuts at: 210 210
Size: 4094
BstYI r'GATC_y
Cuts at: 1 239 1119 1586 1609 1944 2190 3202 3288
Size: 238 880 467 23 335 246 1012 86
Cuts at: 3288 3300 3646 1
Size: 12 346 449
Fragments arranged by size:
1012 880 467 449 346 335 246 238
86 23 12
BstZ17I GTA'TAC
Cuts at: 715 715
Size: 4094
Bsu36I CC'TnA_GG
Cuts at: 637 637
Size: 4094
BtgI C'CryG_G
Cuts at: 215 359 614 693 215
Size: 144 255 79 3616
Fragments arranged by size:
3616 255 144 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2044 2225 2388 2044
Size: 181 163 3750
Fragments arranged by size:
3750 181 163
BtsI GCAGTG_nn'
Cuts at: 256 1337 256
Size: 1081 3013
DraI TTT'AAA
Cuts at: 653 867 1093 653
Size: 214 226 3654
Fragments arranged by size:
3654 226 214
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 153 469 486 536 624 696 1679 1853 2244
Size: 316 17 50 88 72 983 174 391
Cuts at: 2244 2271 3616 153
Size: 27 1345 631
Fragments arranged by size:
1345 983 631 391 316 174 88 72
50 27 17
EagI C'GGCC_G
Cuts at: 469 486 536 1679 469
Size: 17 50 1143 2884
Fragments arranged by size:
2884 1143 50 17
EarI CTCTTCn'nnn_
Cuts at: 277 2117 2327 277
Size: 1840 210 2044
Fragments arranged by size:
2044 1840 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 212 2302 2612 2758 3904 212
Size: 2090 310 146 1146 402
Fragments arranged by size:
2090 1146 402 310 146
EcoICRI GAG'CTC
Cuts at: 1106 1106
Size: 4094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 499 941 1919 2351 3098 3366 314
Size: 185 442 978 432 747 268 1042
Fragments arranged by size:
1042 978 747 442 432 268 185
EcoO109I rG'GnC_Cy
Cuts at: 197 198 525 1233 197
Size: 1 327 708 3058
Fragments arranged by size:
3058 708 327 1
EcoRI G'AATT_C
Cuts at: 839 1098 839
Size: 259 3835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 4094
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 388 1528 1776 2798 3777 388
Size: 1140 248 1022 979 705
Fragments arranged by size:
1140 1022 979 705 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 1936 1966 1968 1998 204
Size: 32 1700 30 2 30 2300
Fragments arranged by size:
2300 1700 32 30 30 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 1524 1772 1524
Size: 248 3846
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2765 2949 1055
Size: 1710 184 2200
Fragments arranged by size:
2200 1710 184
MscI TGG'CCA
Cuts at: 698 1855 698
Size: 1157 2937
MslI CAynn'nnrTG
Cuts at: 722 2083 2210 722
Size: 1361 127 2606
Fragments arranged by size:
2606 1361 127
MspA1I CmG'CkG
Cuts at: 132 435 606 616 1205 1519 1879 2892 3137
Size: 303 171 10 589 314 360 1013 245
Cuts at: 3137 132
Size: 1089
Fragments arranged by size:
1089 1013 589 360 314 303 245 171
10
NaeI GCC'GGC
Cuts at: 573 2275 573
Size: 1702 2392
NarI GG'CG_CC
Cuts at: 1525 1773 1525
Size: 248 3846
NcoI C'CATG_G
Cuts at: 693 693
Size: 4094
NgoMIV G'CCGG_C
Cuts at: 571 2273 571
Size: 1702 2392
NspI r_CATG'y
Cuts at: 1147 1647 2554 1147
Size: 500 907 2687
Fragments arranged by size:
2687 907 500
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PfoI T'CCnGG_A
Cuts at: 242 242
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PpuMI rG'GwC_Cy
Cuts at: 525 525
Size: 4094
PshAI GACnn'nnGTC
Cuts at: 115 115
Size: 4094
PspOMI G'GGCC_C
Cuts at: 197 531 197
Size: 334 3760
PstI C_TGCA'G
Cuts at: 564 1141 1826 564
Size: 577 685 2832
Fragments arranged by size:
2832 685 577
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 435 1519 1879 435
Size: 1084 360 2650
Fragments arranged by size:
2650 1084 360
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 1108 1108
Size: 4094
SacII CC_GC'GG
Cuts at: 617 617
Size: 4094
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 560 1137 1822 2815 3006 33
Size: 527 577 685 993 191 1121
Fragments arranged by size:
1121 993 685 577 527 191
SfoI GGC'GCC
Cuts at: 1526 1774 1526
Size: 248 3846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 253 530 1116 253
Size: 277 586 3231
Fragments arranged by size:
3231 586 277
SmlI C'TyrA_G
Cuts at: 1249 2164 2656 2918 1249
Size: 915 492 262 2425
Fragments arranged by size:
2425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
StyI C'CwwG_G
Cuts at: 288 545 693 1228 1582 288
Size: 257 148 535 354 2800
Fragments arranged by size:
2800 535 354 257 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 139 1677 1810 2269 2478 139
Size: 1538 133 459 209 1755
Fragments arranged by size:
1755 1538 459 209 133
TatI w'GTAC_w
Cuts at: 1056 3609 1056
Size: 2553 1541
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4062
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 706 1589 706
Size: 883 3211
XmaI C'CCGG_G
Cuts at: 251 528 1114 251
Size: 277 586 3231
Fragments arranged by size:
3231 586 277
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstXI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI