(Circular) (Six-base) MAPSORT of: pF1KB8960.seq Check: 64 from: 1 to: 4133 pF1KB8960 4133 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 341 1171 341 Size: 830 3303 Acc65I G'GTAC_C Cuts at: 1149 1149 Size: 4133 AclI AA'CG_TT Cuts at: 3973 3973 Size: 4133 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1958 2390 3137 3405 1958 Size: 432 747 268 2686 Fragments arranged by size: 2686 747 432 268 AflIII A'CryG_T Cuts at: 1485 2589 3566 1485 Size: 1104 977 2052 Fragments arranged by size: 2052 1104 977 AgeI A'CCGG_T Cuts at: 2521 2521 Size: 4133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 229 229 Size: 4133 AlwNI CAG_nnn'CTG Cuts at: 170 507 1438 3005 170 Size: 337 931 1567 1298 Fragments arranged by size: 1567 1298 931 337 ApaLI G'TGCA_C Cuts at: 2903 2903 Size: 4133 ApoI r'AATT_y Cuts at: 1137 3486 3742 1137 Size: 2349 256 1528 Fragments arranged by size: 2349 1528 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4133 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4133 AvaI C'yCGr_G Cuts at: 763 1153 763 Size: 390 3743 BamHI G'GATC_C Cuts at: 1094 1158 1094 Size: 64 4069 BanI G'GyrC_C Cuts at: 917 1149 1563 1811 1846 917 Size: 232 414 248 35 3204 Fragments arranged by size: 3204 414 248 232 35 BanII G_rGCy'C Cuts at: 719 737 763 1147 2177 719 Size: 18 26 384 1030 2675 Fragments arranged by size: 2675 1030 384 26 18 BbeI G_GCGC'C Cuts at: 1567 1815 1567 Size: 248 3885 BbvCI CC'TCA_GC Cuts at: 806 806 Size: 4133 BciVI GTATCCnnnnn_n' Cuts at: 2031 2792 4133 2031 Size: 761 1341 2031 Fragments arranged by size: 2031 1341 761 BclI T'GATC_A Cuts at: 161 1653 161 Size: 1492 2641 BglI GCCn_nnn'nGGC Cuts at: 620 620 Size: 4133 BglII A'GATC_T Cuts at: 364 1648 3685 364 Size: 1284 2037 812 Fragments arranged by size: 2037 1284 812 BlpI GC'TnA_GC Cuts at: 1245 1245 Size: 4133 Bme1580I G_kGCm'C Cuts at: 922 1758 1851 2907 922 Size: 836 93 1056 2148 Fragments arranged by size: 2148 1056 836 93 BmrI ACTGGGnnnn_n' Cuts at: 1345 1426 1761 3905 1345 Size: 81 335 2144 1573 Fragments arranged by size: 2144 1573 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 4133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 625 625 Size: 4133 Bpu10I CC'TnA_GC Cuts at: 806 806 Size: 4133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 320 1054 1309 2188 2680 2978 320 Size: 734 255 879 492 298 1475 Fragments arranged by size: 1475 879 734 492 298 255 BsaI GGTCTCn'nnnn_ Cuts at: 816 3837 816 Size: 3021 1112 BsaAI yAC'GTr Cuts at: 1486 1486 Size: 4133 BsaBI GATnn'nnATC Cuts at: 6 1647 3684 6 Size: 1641 2037 455 Fragments arranged by size: 2037 1641 455 BsaHI Gr'CG_yC Cuts at: 1564 1812 2514 4010 1564 Size: 248 702 1496 1687 Fragments arranged by size: 1687 1496 702 248 BsaWI w'CCGG_w Cuts at: 1091 1322 1843 2521 2795 2942 3353 1091 Size: 231 521 678 274 147 411 1871 Fragments arranged by size: 1871 678 521 411 274 231 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 168 198 168 Size: 30 4103 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 807 807 Size: 4133 BseYI C'CCAG_C Cuts at: 686 1558 2316 2893 3389 686 Size: 872 758 577 496 1430 Fragments arranged by size: 1430 872 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 853 853 Size: 4133 BsiEI CG_ry'CG Cuts at: 104 485 1675 1721 2929 104 Size: 381 1190 46 1208 1308 Fragments arranged by size: 1308 1208 1190 381 46 BsiHKAI G_wGCw'C Cuts at: 1147 1925 2115 2907 1147 Size: 778 190 792 2373 Fragments arranged by size: 2373 792 778 190 BsmI GAATG_Cn' Cuts at: 254 254 Size: 4133 BsmBI CGTCTCn'nnnn_ Cuts at: 278 278 Size: 4133 Bsp1286I G_dGCh'C Cuts at: 719 737 763 922 1147 1758 1851 1925 2115 Size: 18 26 159 225 611 93 74 190 Cuts at: 2115 2177 2907 719 Size: 62 730 1945 Fragments arranged by size: 1945 730 611 225 190 159 93 74 62 26 18 BspEI T'CCGG_A Cuts at: 3353 3353 Size: 4133 BspHI T'CATG_A Cuts at: 3309 3309 Size: 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1023 1183 1397 1699 2080 1023 Size: 160 214 302 381 3076 Fragments arranged by size: 3076 381 302 214 160 BsrBI CCG'CTC Cuts at: 50 725 2425 2479 3963 4128 50 Size: 675 1700 54 1484 165 55 Fragments arranged by size: 1700 1484 675 165 55 54 BsrDI GCAATG_nn' Cuts at: 2045 2045 Size: 4133 BsrFI r'CCGG_y Cuts at: 2131 2312 2521 2131 Size: 181 209 3743 Fragments arranged by size: 3743 209 181 BsrGI T'GTAC_A Cuts at: 209 209 Size: 4133 BssSI C'ACGA_G Cuts at: 653 2404 2762 653 Size: 1751 358 2024 Fragments arranged by size: 2024 1751 358 BstAPI GCAn_nnn'nTGC Cuts at: 1510 1510 Size: 4133 BstBI TT'CG_AA Cuts at: 2494 2494 Size: 4133 BstYI r'GATC_y Cuts at: 1 354 364 1094 1158 1625 1648 1983 2229 Size: 353 10 730 64 467 23 335 246 Cuts at: 2229 3241 3327 3339 3685 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 730 467 449 353 346 335 246 86 64 23 12 10 Bsu36I CC'TnA_GG Cuts at: 539 539 Size: 4133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2083 2264 2427 2083 Size: 181 163 3789 Fragments arranged by size: 3789 181 163 BtsI GCAGTG_nn' Cuts at: 890 1376 890 Size: 486 3647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 636 671 636 Size: 35 4098 DraI TTT'AAA Cuts at: 1132 1132 Size: 4133 DrdI GACnn_nn'nnGTC Cuts at: 1033 1839 2697 1033 Size: 806 858 2469 Fragments arranged by size: 2469 858 806 EaeI y'GGCC_r Cuts at: 278 482 662 726 1718 1892 2283 2310 3655 Size: 204 180 64 992 174 391 27 1345 Cuts at: 3655 278 Size: 756 Fragments arranged by size: 1345 992 756 391 204 180 174 64 27 EagI C'GGCC_G Cuts at: 482 1718 482 Size: 1236 2897 EarI CTCTTCn'nnn_ Cuts at: 782 907 2156 2366 782 Size: 125 1249 210 2549 Fragments arranged by size: 2549 1249 210 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2341 2651 2797 3943 2341 Size: 310 146 1146 2531 Fragments arranged by size: 2531 1146 310 146 EcoICRI GAG'CTC Cuts at: 1145 1145 Size: 4133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 625 1958 2390 3137 3405 625 Size: 1333 432 747 268 1353 Fragments arranged by size: 1353 1333 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 805 805 Size: 4133 EcoO109I rG'GnC_Cy Cuts at: 239 489 1272 239 Size: 250 783 3100 Fragments arranged by size: 3100 783 250 EcoRI G'AATT_C Cuts at: 1137 1137 Size: 4133 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 796 2152 796 Size: 1356 2777 FspI TGC'GCA Cuts at: 1914 1914 Size: 4133 HaeII r_GCGC'y Cuts at: 203 993 1567 1815 2837 3816 203 Size: 790 574 248 1022 979 520 Fragments arranged by size: 1022 979 790 574 520 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1975 2005 2007 2037 1975 Size: 30 2 30 4071 Fragments arranged by size: 4071 30 30 2 HincII GTy'rAC Cuts at: 1017 1172 1017 Size: 155 3978 HpaI GTT'AAC Cuts at: 1017 1017 Size: 4133 KasI G'GCGC_C Cuts at: 1563 1811 1563 Size: 248 3885 KpnI G_GTAC'C Cuts at: 1153 1153 Size: 4133 MfeI C'AATT_G Cuts at: 441 882 441 Size: 441 3692 MluI A'CGCG_T Cuts at: 3566 3566 Size: 4133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 328 2804 2988 328 Size: 2476 184 1473 Fragments arranged by size: 2476 1473 184 MscI TGG'CCA Cuts at: 280 1894 280 Size: 1614 2519 MslI CAynn'nnrTG Cuts at: 211 634 2122 2249 211 Size: 423 1488 127 2095 Fragments arranged by size: 2095 1488 423 127 MspA1I CmG'CkG Cuts at: 682 690 1244 1558 1918 2931 3176 682 Size: 8 554 314 360 1013 245 1639 Fragments arranged by size: 1639 1013 554 360 314 245 8 NaeI GCC'GGC Cuts at: 2314 2314 Size: 4133 NarI GG'CG_CC Cuts at: 1564 1812 1564 Size: 248 3885 NgoMIV G'CCGG_C Cuts at: 2312 2312 Size: 4133 NspI r_CATG'y Cuts at: 210 216 453 1186 1686 2593 210 Size: 6 237 733 500 907 1750 Fragments arranged by size: 1750 907 733 500 237 6 PciI A'CATG_T Cuts at: 2589 2589 Size: 4133 PflMI CCAn_nnn'nTGG Cuts at: 306 507 884 942 306 Size: 201 377 58 3497 Fragments arranged by size: 3497 377 201 58 PmeI GTTT'AAAC Cuts at: 1132 1132 Size: 4133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 949 981 3272 3304 949 Size: 32 2291 32 1778 Fragments arranged by size: 2291 1778 32 32 PpuMI rG'GwC_Cy Cuts at: 239 489 239 Size: 250 3883 PstI C_TGCA'G Cuts at: 175 695 1180 1865 175 Size: 520 485 685 2443 Fragments arranged by size: 2443 685 520 485 PvuI CG_AT'CG Cuts at: 104 104 Size: 4133 PvuII CAG'CTG Cuts at: 690 1558 1918 690 Size: 868 360 2905 Fragments arranged by size: 2905 868 360 RsrII CG'GwC_CG Cuts at: 2328 2328 Size: 4133 SacI G_AGCT'C Cuts at: 1147 1147 Size: 4133 SalI G'TCGA_C Cuts at: 1170 1170 Size: 4133 SapI GCTCTTCn'nnn_ Cuts at: 2156 2366 2156 Size: 210 3923 SbfI CC_TGCA'GG Cuts at: 175 1180 175 Size: 1005 3128 ScaI AGT'ACT Cuts at: 3650 3650 Size: 4133 SfcI C'TryA_G Cuts at: 33 171 691 1176 1861 2854 3045 33 Size: 138 520 485 685 993 191 1121 Fragments arranged by size: 1121 993 685 520 485 191 138 SfoI GGC'GCC Cuts at: 1565 1813 1565 Size: 248 3885 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4133 SmaI CCC'GGG Cuts at: 1155 1155 Size: 4133 SmlI C'TyrA_G Cuts at: 335 1069 1288 2203 2695 2957 335 Size: 734 219 915 492 262 1511 Fragments arranged by size: 1511 915 734 492 262 219 SpeI A'CTAG_T Cuts at: 67 67 Size: 4133 SphI G_CATG'C Cuts at: 1186 1686 1186 Size: 500 3633 StyI C'CwwG_G Cuts at: 1267 1621 1267 Size: 354 3779 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1716 1849 2308 2517 1716 Size: 133 459 209 3332 Fragments arranged by size: 3332 459 209 133 TatI w'GTAC_w Cuts at: 209 602 3648 209 Size: 393 3046 694 Fragments arranged by size: 3046 694 393 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 383 415 3650 3682 383 Size: 32 3235 32 834 Fragments arranged by size: 3235 834 32 32 Tth111I GACn'n_nGTC Cuts at: 569 1930 569 Size: 1361 2772 XbaI T'CTAG_A Cuts at: 1164 1164 Size: 4133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 881 1628 881 Size: 747 3386 XmaI C'CCGG_G Cuts at: 1153 1153 Size: 4133 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BssHII BstEII BstXI BstZ17I BtgI ClaI DraIII EcoRV FseI FspAI HindIII NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI