Restriction map for pF1KB8960
 (Circular) (Six-base) MAPSORT of: pF1KB8960.seq  Check: 64  from: 1  to: 4133



pF1KB8960  4133 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:    341    1171     341 
   Size:        830    3303
 
Acc65I G'GTAC_C

Cuts at:   1149    1149 
   Size:       4133
 
AclI AA'CG_TT

Cuts at:   3973    3973 
   Size:       4133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1958    2390    3137    3405    1958 
   Size:        432     747     268    2686

  Fragments arranged by size: 

                2686     747     432     268
 
AflIII A'CryG_T

Cuts at:   1485    2589    3566    1485 
   Size:       1104     977    2052

  Fragments arranged by size: 

                2052    1104     977
 
AgeI A'CCGG_T

Cuts at:   2521    2521 
   Size:       4133
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    229     229 
   Size:       4133
 
AlwNI CAG_nnn'CTG

Cuts at:    170     507    1438    3005     170 
   Size:        337     931    1567    1298

  Fragments arranged by size: 

                1567    1298     931     337
 
ApaLI G'TGCA_C

Cuts at:   2903    2903 
   Size:       4133
 
ApoI r'AATT_y

Cuts at:   1137    3486    3742    1137 
   Size:       2349     256    1528

  Fragments arranged by size: 

                2349    1528     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4133
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4133
 
AvaI C'yCGr_G

Cuts at:    763    1153     763 
   Size:        390    3743
 
BamHI G'GATC_C

Cuts at:   1094    1158    1094 
   Size:         64    4069
 
BanI G'GyrC_C

Cuts at:    917    1149    1563    1811    1846     917 
   Size:        232     414     248      35    3204

  Fragments arranged by size: 

                3204     414     248     232      35
 
BanII G_rGCy'C

Cuts at:    719     737     763    1147    2177     719 
   Size:         18      26     384    1030    2675

  Fragments arranged by size: 

                2675    1030     384      26      18
 
BbeI G_GCGC'C

Cuts at:   1567    1815    1567 
   Size:        248    3885
 
BbvCI CC'TCA_GC

Cuts at:    806     806 
   Size:       4133
 
BciVI GTATCCnnnnn_n'

Cuts at:   2031    2792    4133    2031 
   Size:        761    1341    2031

  Fragments arranged by size: 

                2031    1341     761
 
BclI T'GATC_A

Cuts at:    161    1653     161 
   Size:       1492    2641
 
BglI GCCn_nnn'nGGC

Cuts at:    620     620 
   Size:       4133
 
BglII A'GATC_T

Cuts at:    364    1648    3685     364 
   Size:       1284    2037     812

  Fragments arranged by size: 

                2037    1284     812
 
BlpI GC'TnA_GC

Cuts at:   1245    1245 
   Size:       4133
 
Bme1580I G_kGCm'C

Cuts at:    922    1758    1851    2907     922 
   Size:        836      93    1056    2148

  Fragments arranged by size: 

                2148    1056     836      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1345    1426    1761    3905    1345 
   Size:         81     335    2144    1573

  Fragments arranged by size: 

                2144    1573     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2500    2500 
   Size:       4133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    625     625 
   Size:       4133
 
Bpu10I CC'TnA_GC

Cuts at:    806     806 
   Size:       4133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    320    1054    1309    2188    2680    2978     320 
   Size:        734     255     879     492     298    1475

  Fragments arranged by size: 

                1475     879     734     492     298     255
 
BsaI GGTCTCn'nnnn_

Cuts at:    816    3837     816 
   Size:       3021    1112
 
BsaAI yAC'GTr

Cuts at:   1486    1486 
   Size:       4133
 
BsaBI GATnn'nnATC

Cuts at:      6    1647    3684       6 
   Size:       1641    2037     455

  Fragments arranged by size: 

                2037    1641     455
 
BsaHI Gr'CG_yC

Cuts at:   1564    1812    2514    4010    1564 
   Size:        248     702    1496    1687

  Fragments arranged by size: 

                1687    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1091    1322    1843    2521    2795    2942    3353    1091 
   Size:        231     521     678     274     147     411    1871

  Fragments arranged by size: 

                1871     678     521     411     274     231     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    168     198     168 
   Size:         30    4103
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    807     807 
   Size:       4133
 
BseYI C'CCAG_C

Cuts at:    686    1558    2316    2893    3389     686 
   Size:        872     758     577     496    1430

  Fragments arranged by size: 

                1430     872     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    853     853 
   Size:       4133
 
BsiEI CG_ry'CG

Cuts at:    104     485    1675    1721    2929     104 
   Size:        381    1190      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1190     381      46
 
BsiHKAI G_wGCw'C

Cuts at:   1147    1925    2115    2907    1147 
   Size:        778     190     792    2373

  Fragments arranged by size: 

                2373     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    254     254 
   Size:       4133
 
BsmBI CGTCTCn'nnnn_

Cuts at:    278     278 
   Size:       4133
 
Bsp1286I G_dGCh'C

Cuts at:    719     737     763     922    1147    1758    1851    1925    2115 
   Size:         18      26     159     225     611      93      74     190

Cuts at:   2115    2177    2907     719 
   Size:         62     730    1945

  Fragments arranged by size: 

                1945     730     611     225     190     159      93      74
                  62      26      18
 
BspEI T'CCGG_A

Cuts at:   3353    3353 
   Size:       4133
 
BspHI T'CATG_A

Cuts at:   3309    3309 
   Size:       4133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1023    1183    1397    1699    2080    1023 
   Size:        160     214     302     381    3076

  Fragments arranged by size: 

                3076     381     302     214     160
 
BsrBI CCG'CTC

Cuts at:     50     725    2425    2479    3963    4128      50 
   Size:        675    1700      54    1484     165      55

  Fragments arranged by size: 

                1700    1484     675     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2045    2045 
   Size:       4133
 
BsrFI r'CCGG_y

Cuts at:   2131    2312    2521    2131 
   Size:        181     209    3743

  Fragments arranged by size: 

                3743     209     181
 
BsrGI T'GTAC_A

Cuts at:    209     209 
   Size:       4133
 
BssSI C'ACGA_G

Cuts at:    653    2404    2762     653 
   Size:       1751     358    2024

  Fragments arranged by size: 

                2024    1751     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1510    1510 
   Size:       4133
 
BstBI TT'CG_AA

Cuts at:   2494    2494 
   Size:       4133
 
BstYI r'GATC_y

Cuts at:      1     354     364    1094    1158    1625    1648    1983    2229 
   Size:        353      10     730      64     467      23     335     246

Cuts at:   2229    3241    3327    3339    3685       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     730     467     449     353     346     335     246
                  86      64      23      12      10
 
Bsu36I CC'TnA_GG

Cuts at:    539     539 
   Size:       4133
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2083    2264    2427    2083 
   Size:        181     163    3789

  Fragments arranged by size: 

                3789     181     163
 
BtsI GCAGTG_nn'

Cuts at:    890    1376     890 
   Size:        486    3647
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    636     671     636 
   Size:         35    4098
 
DraI TTT'AAA

Cuts at:   1132    1132 
   Size:       4133
 
DrdI GACnn_nn'nnGTC

Cuts at:   1033    1839    2697    1033 
   Size:        806     858    2469

  Fragments arranged by size: 

                2469     858     806
 
EaeI y'GGCC_r

Cuts at:    278     482     662     726    1718    1892    2283    2310    3655 
   Size:        204     180      64     992     174     391      27    1345

Cuts at:   3655     278 
   Size:        756

  Fragments arranged by size: 

                1345     992     756     391     204     180     174      64
                  27
 
EagI C'GGCC_G

Cuts at:    482    1718     482 
   Size:       1236    2897
 
EarI CTCTTCn'nnn_

Cuts at:    782     907    2156    2366     782 
   Size:        125    1249     210    2549

  Fragments arranged by size: 

                2549    1249     210     125
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2341    2651    2797    3943    2341 
   Size:        310     146    1146    2531

  Fragments arranged by size: 

                2531    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1145    1145 
   Size:       4133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    625    1958    2390    3137    3405     625 
   Size:       1333     432     747     268    1353

  Fragments arranged by size: 

                1353    1333     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    805     805 
   Size:       4133
 
EcoO109I rG'GnC_Cy

Cuts at:    239     489    1272     239 
   Size:        250     783    3100

  Fragments arranged by size: 

                3100     783     250
 
EcoRI G'AATT_C

Cuts at:   1137    1137 
   Size:       4133
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    796    2152     796 
   Size:       1356    2777
 
FspI TGC'GCA

Cuts at:   1914    1914 
   Size:       4133
 
HaeII r_GCGC'y

Cuts at:    203     993    1567    1815    2837    3816     203 
   Size:        790     574     248    1022     979     520

  Fragments arranged by size: 

                1022     979     790     574     520     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1975    2005    2007    2037    1975 
   Size:         30       2      30    4071

  Fragments arranged by size: 

                4071      30      30       2
 
HincII GTy'rAC

Cuts at:   1017    1172    1017 
   Size:        155    3978
 
HpaI GTT'AAC

Cuts at:   1017    1017 
   Size:       4133
 
KasI G'GCGC_C

Cuts at:   1563    1811    1563 
   Size:        248    3885
 
KpnI G_GTAC'C

Cuts at:   1153    1153 
   Size:       4133
 
MfeI C'AATT_G

Cuts at:    441     882     441 
   Size:        441    3692
 
MluI A'CGCG_T

Cuts at:   3566    3566 
   Size:       4133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    328    2804    2988     328 
   Size:       2476     184    1473

  Fragments arranged by size: 

                2476    1473     184
 
MscI TGG'CCA

Cuts at:    280    1894     280 
   Size:       1614    2519
 
MslI CAynn'nnrTG

Cuts at:    211     634    2122    2249     211 
   Size:        423    1488     127    2095

  Fragments arranged by size: 

                2095    1488     423     127
 
MspA1I CmG'CkG

Cuts at:    682     690    1244    1558    1918    2931    3176     682 
   Size:          8     554     314     360    1013     245    1639

  Fragments arranged by size: 

                1639    1013     554     360     314     245       8
 
NaeI GCC'GGC

Cuts at:   2314    2314 
   Size:       4133
 
NarI GG'CG_CC

Cuts at:   1564    1812    1564 
   Size:        248    3885
 
NgoMIV G'CCGG_C

Cuts at:   2312    2312 
   Size:       4133
 
NspI r_CATG'y

Cuts at:    210     216     453    1186    1686    2593     210 
   Size:          6     237     733     500     907    1750

  Fragments arranged by size: 

                1750     907     733     500     237       6
 
PciI A'CATG_T

Cuts at:   2589    2589 
   Size:       4133
 
PflMI CCAn_nnn'nTGG

Cuts at:    306     507     884     942     306 
   Size:        201     377      58    3497

  Fragments arranged by size: 

                3497     377     201      58
 
PmeI GTTT'AAAC

Cuts at:   1132    1132 
   Size:       4133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    949     981    3272    3304     949 
   Size:         32    2291      32    1778

  Fragments arranged by size: 

                2291    1778      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    239     489     239 
   Size:        250    3883
 
PstI C_TGCA'G

Cuts at:    175     695    1180    1865     175 
   Size:        520     485     685    2443

  Fragments arranged by size: 

                2443     685     520     485
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4133
 
PvuII CAG'CTG

Cuts at:    690    1558    1918     690 
   Size:        868     360    2905

  Fragments arranged by size: 

                2905     868     360
 
RsrII CG'GwC_CG

Cuts at:   2328    2328 
   Size:       4133
 
SacI G_AGCT'C

Cuts at:   1147    1147 
   Size:       4133
 
SalI G'TCGA_C

Cuts at:   1170    1170 
   Size:       4133
 
SapI GCTCTTCn'nnn_

Cuts at:   2156    2366    2156 
   Size:        210    3923
 
SbfI CC_TGCA'GG

Cuts at:    175    1180     175 
   Size:       1005    3128
 
ScaI AGT'ACT

Cuts at:   3650    3650 
   Size:       4133
 
SfcI C'TryA_G

Cuts at:     33     171     691    1176    1861    2854    3045      33 
   Size:        138     520     485     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     520     485     191     138
 
SfoI GGC'GCC

Cuts at:   1565    1813    1565 
   Size:        248    3885
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4133
 
SmaI CCC'GGG

Cuts at:   1155    1155 
   Size:       4133
 
SmlI C'TyrA_G

Cuts at:    335    1069    1288    2203    2695    2957     335 
   Size:        734     219     915     492     262    1511

  Fragments arranged by size: 

                1511     915     734     492     262     219
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4133
 
SphI G_CATG'C

Cuts at:   1186    1686    1186 
   Size:        500    3633
 
StyI C'CwwG_G

Cuts at:   1267    1621    1267 
   Size:        354    3779
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1716    1849    2308    2517    1716 
   Size:        133     459     209    3332

  Fragments arranged by size: 

                3332     459     209     133
 
TatI w'GTAC_w

Cuts at:    209     602    3648     209 
   Size:        393    3046     694

  Fragments arranged by size: 

                3046     694     393
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    383     415    3650    3682     383 
   Size:         32    3235      32     834

  Fragments arranged by size: 

                3235     834      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    569    1930     569 
   Size:       1361    2772
 
XbaI T'CTAG_A

Cuts at:   1164    1164 
   Size:       4133
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    881    1628     881 
   Size:        747    3386
 
XmaI C'CCGG_G

Cuts at:   1153    1153 
   Size:       4133
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI   Bsu36I    BtgZI
     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI    BmgBI     BmtI
    BsiWI   BssHII   BstEII    BstXI  BstZ17I     BtgI     ClaI   DraIII
    EcoRV     FseI    FspAI  HindIII     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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