Restriction map for
pF1KB8969
(Circular) (Six-base) MAPSORT of: pF1KB8969.seq Check: 1887 from: 1 to: 4190
pF1KB8969 4190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
AflII C'TTAA_G
Cuts at: 1042 1042
Size: 4190
AflIII A'CryG_T
Cuts at: 558 1542 2646 3623 558
Size: 984 1104 977 1125
Fragments arranged by size:
1125 1104 984 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 861 1495 3062 861
Size: 634 1567 1989
Fragments arranged by size:
1989 1567 634
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 641 799 1125 1194 3543 3799 641
Size: 158 326 69 2349 256 1032
Fragments arranged by size:
2349 1032 326 256 158 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 401 1215 401
Size: 814 3376
BanI G'GyrC_C
Cuts at: 1206 1620 1868 1903 1206
Size: 414 248 35 3493
Fragments arranged by size:
3493 414 248 35
BanII G_rGCy'C
Cuts at: 737 898 1204 2234 737
Size: 161 306 1030 2693
Fragments arranged by size:
2693 1030 306 161
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 686 686
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglII A'GATC_T
Cuts at: 829 1705 3742 829
Size: 876 2037 1277
Fragments arranged by size:
2037 1277 876
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 472 1815 1908 2964 472
Size: 1343 93 1056 1698
Fragments arranged by size:
1698 1343 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 702 1402 1483 1818 3962 702
Size: 700 81 335 2144 930
Fragments arranged by size:
2144 930 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 649 1621 1869 2571 4067 649
Size: 972 248 702 1496 772
Fragments arranged by size:
1496 972 772 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1070 1100 1070
Size: 30 4160
BseYI C'CCAG_C
Cuts at: 1615 2373 2950 3446 1615
Size: 758 577 496 2359
Fragments arranged by size:
2359 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1204 1982 2172 2964 1204
Size: 778 190 792 2430
Fragments arranged by size:
2430 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 472 737 898 1204 1815 1908 1982 2172 2234
Size: 265 161 306 611 93 74 190 62
Cuts at: 2234 2964 472
Size: 730 1698
Fragments arranged by size:
1698 730 611 306 265 190 161 93
74 62
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 215 260 3366 215
Size: 45 3106 1039
Fragments arranged by size:
3106 1039 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 178 1240 1454 1756 2137 178
Size: 1062 214 302 381 2231
Fragments arranged by size:
2231 1062 381 302 214
BsrBI CCG'CTC
Cuts at: 50 504 2482 2536 4020 4185 50
Size: 454 1978 54 1484 165 55
Fragments arranged by size:
1978 1484 454 165 55 54
BsrDI GCAATG_nn'
Cuts at: 771 2102 771
Size: 1331 2859
BsrFI r'CCGG_y
Cuts at: 185 2188 2369 2578 185
Size: 2003 181 209 1797
Fragments arranged by size:
2003 1797 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 324 324
Size: 4190
BstYI r'GATC_y
Cuts at: 1 401 829 1005 1215 1682 1705 2040 2286
Size: 400 428 176 210 467 23 335 246
Cuts at: 2286 3298 3384 3396 3742 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 428 400 346 335 246
210 176 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 2140 2321 2484 234
Size: 1906 181 163 1940
Fragments arranged by size:
1940 1906 181 163
BtsI GCAGTG_nn'
Cuts at: 492 1433 492
Size: 941 3249
DraI TTT'AAA
Cuts at: 804 1189 804
Size: 385 3805
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 314 345 494 914 1775 1949 2340 2367 3712
Size: 31 149 420 861 174 391 27 1345
Cuts at: 3712 314
Size: 792
Fragments arranged by size:
1345 861 792 420 391 174 149 31
27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 656 1329 656
Size: 673 3517
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1070 1102 2032 2062 2064 2094 1070
Size: 32 930 30 2 30 3166
Fragments arranged by size:
3166 930 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
HindIII A'AGCT_T
Cuts at: 1057 1057
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 871 2861 3045 871
Size: 1990 184 2016
Fragments arranged by size:
2016 1990 184
MscI TGG'CCA
Cuts at: 316 347 496 916 1951 316
Size: 31 149 420 1035 2555
Fragments arranged by size:
2555 1035 420 149 31
MslI CAynn'nnrTG
Cuts at: 112 220 259 759 2179 2306 112
Size: 108 39 500 1420 127 1996
Fragments arranged by size:
1996 1420 500 127 108 39
MspA1I CmG'CkG
Cuts at: 148 1301 1615 1975 2988 3233 148
Size: 1153 314 360 1013 245 1105
Fragments arranged by size:
1153 1105 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 562 1087 1243 1743 2650 562
Size: 525 156 500 907 2102
Fragments arranged by size:
2102 907 525 500 156
PciI A'CATG_T
Cuts at: 558 2646 558
Size: 2088 2102
PflMI CCAn_nnn'nTGG
Cuts at: 861 861
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PpuMI rG'GwC_Cy
Cuts at: 656 656
Size: 4190
PshAI GACnn'nnGTC
Cuts at: 288 288
Size: 4190
PstI C_TGCA'G
Cuts at: 147 1237 1922 147
Size: 1090 685 2415
Fragments arranged by size:
2415 1090 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 148 1615 1975 148
Size: 1467 360 2363
Fragments arranged by size:
2363 1467 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SexAI A'CCwGG_T
Cuts at: 658 658
Size: 4190
SfcI C'TryA_G
Cuts at: 33 143 1233 1918 2911 3102 33
Size: 110 1090 685 993 191 1121
Fragments arranged by size:
1121 1090 993 685 191 110
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1042 1345 2260 2752 3014 1042
Size: 303 915 492 262 2218
Fragments arranged by size:
2218 915 492 303 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
SspI AAT'ATT
Cuts at: 530 530
Size: 4190
StyI C'CwwG_G
Cuts at: 293 621 744 1009 1324 1678 293
Size: 328 123 265 315 354 2805
Fragments arranged by size:
2805 354 328 315 265 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 1906 2365 2574 1773
Size: 133 459 209 3389
Fragments arranged by size:
3389 459 209 133
TatI w'GTAC_w
Cuts at: 3705 3705
Size: 4190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 355 1685 355
Size: 1330 2860
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
XmnI GAAnn'nnTTC
Cuts at: 939 939
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI