(Circular) (Six-base) MAPSORT of: pF1KB8970.seq Check: 3280 from: 1 to: 4190 pF1KB8970 4190 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 1228 1228 Size: 4190 Acc65I G'GTAC_C Cuts at: 1206 1206 Size: 4190 AclI AA'CG_TT Cuts at: 4030 4030 Size: 4190 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 171 1028 2015 2447 3194 3462 171 Size: 857 987 432 747 268 899 Fragments arranged by size: 987 899 857 747 432 268 AflIII A'CryG_T Cuts at: 1542 2646 3623 1542 Size: 1104 977 2109 Fragments arranged by size: 2109 1104 977 AgeI A'CCGG_T Cuts at: 2578 2578 Size: 4190 AleI CACnn'nnGTG Cuts at: 145 492 145 Size: 347 3843 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 510 510 Size: 4190 AlwNI CAG_nnn'CTG Cuts at: 833 879 1495 3062 833 Size: 46 616 1567 1961 Fragments arranged by size: 1961 1567 616 46 ApaLI G'TGCA_C Cuts at: 2960 2960 Size: 4190 ApoI r'AATT_y Cuts at: 1194 3543 3799 1194 Size: 2349 256 1585 Fragments arranged by size: 2349 1585 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4190 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4190 AvaI C'yCGr_G Cuts at: 237 1210 237 Size: 973 3217 AvrII C'CTAG_G Cuts at: 507 507 Size: 4190 BamHI G'GATC_C Cuts at: 1215 1215 Size: 4190 BanI G'GyrC_C Cuts at: 512 646 766 809 1206 1620 1868 1903 512 Size: 134 120 43 397 414 248 35 2799 Fragments arranged by size: 2799 414 397 248 134 120 43 35 BanII G_rGCy'C Cuts at: 458 685 1204 2234 458 Size: 227 519 1030 2414 Fragments arranged by size: 2414 1030 519 227 BbeI G_GCGC'C Cuts at: 770 1624 1872 770 Size: 854 248 3088 Fragments arranged by size: 3088 854 248 BbsI GAAGACnn'nnnn_ Cuts at: 279 1025 279 Size: 746 3444 BbvCI CC'TCA_GC Cuts at: 872 872 Size: 4190 BciVI GTATCCnnnnn_n' Cuts at: 2088 2849 4190 2088 Size: 761 1341 2088 Fragments arranged by size: 2088 1341 761 BclI T'GATC_A Cuts at: 1710 1710 Size: 4190 BglI GCCn_nnn'nGGC Cuts at: 203 203 Size: 4190 BglII A'GATC_T Cuts at: 1087 1705 3742 1087 Size: 618 2037 1535 Fragments arranged by size: 2037 1535 618 BlpI GC'TnA_GC Cuts at: 1302 1302 Size: 4190 Bme1580I G_kGCm'C Cuts at: 515 1815 1908 2964 515 Size: 1300 93 1056 1741 Fragments arranged by size: 1741 1300 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1402 1483 1818 3962 1402 Size: 81 335 2144 1630 Fragments arranged by size: 2144 1630 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 699 2557 699 Size: 1858 2332 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 511 702 901 1076 511 Size: 191 199 175 3625 Fragments arranged by size: 3625 199 191 175 Bpu10I CC'TnA_GC Cuts at: 343 872 343 Size: 529 3661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1366 2245 2737 3035 1366 Size: 879 492 298 2521 Fragments arranged by size: 2521 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3894 3894 Size: 4190 BsaAI yAC'GTr Cuts at: 1543 1543 Size: 4190 BsaBI GATnn'nnATC Cuts at: 6 1041 1075 1704 3741 6 Size: 1035 34 629 2037 455 Fragments arranged by size: 2037 1035 629 455 34 BsaHI Gr'CG_yC Cuts at: 261 767 1621 1869 2571 4067 261 Size: 506 854 248 702 1496 384 Fragments arranged by size: 1496 854 702 506 384 248 BsaWI w'CCGG_w Cuts at: 377 1379 1900 2578 2852 2999 3410 377 Size: 1002 521 678 274 147 411 1157 Fragments arranged by size: 1157 1002 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 458 488 802 832 458 Size: 30 314 30 3816 Fragments arranged by size: 3816 314 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 450 691 898 450 Size: 241 207 3742 Fragments arranged by size: 3742 241 207 BseYI C'CCAG_C Cuts at: 243 327 696 1615 2373 2950 3446 243 Size: 84 369 919 758 577 496 987 Fragments arranged by size: 987 919 758 577 496 369 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 652 807 1173 652 Size: 155 366 3669 Fragments arranged by size: 3669 366 155 BsiEI CG_ry'CG Cuts at: 104 1732 1778 2986 104 Size: 1628 46 1208 1308 Fragments arranged by size: 1628 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 185 256 713 926 1204 1982 2172 2964 185 Size: 71 457 213 278 778 190 792 1411 Fragments arranged by size: 1411 792 778 457 278 213 190 71 Bsp1286I G_dGCh'C Cuts at: 185 256 458 515 685 713 926 1204 1815 Size: 71 202 57 170 28 213 278 611 Cuts at: 1815 1908 1982 2172 2234 2964 185 Size: 93 74 190 62 730 1411 Fragments arranged by size: 1411 730 611 278 213 202 190 170 93 74 71 62 57 28 BspEI T'CCGG_A Cuts at: 3410 3410 Size: 4190 BspHI T'CATG_A Cuts at: 3366 3366 Size: 4190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 218 1240 1454 1756 2137 218 Size: 1022 214 302 381 2271 Fragments arranged by size: 2271 1022 381 302 214 BsrBI CCG'CTC Cuts at: 50 2482 2536 4020 4185 50 Size: 2432 54 1484 165 55 Fragments arranged by size: 2432 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2102 2102 Size: 4190 BsrFI r'CCGG_y Cuts at: 193 794 1148 2188 2369 2578 193 Size: 601 354 1040 181 209 1805 Fragments arranged by size: 1805 1040 601 354 209 181 BssHII G'CGCG_C Cuts at: 232 599 846 232 Size: 367 247 3576 Fragments arranged by size: 3576 367 247 BssSI C'ACGA_G Cuts at: 673 2461 2819 673 Size: 1788 358 2044 Fragments arranged by size: 2044 1788 358 BstAPI GCAn_nnn'nTGC Cuts at: 653 1567 653 Size: 914 3276 BstBI TT'CG_AA Cuts at: 2551 2551 Size: 4190 BstEII G'GTnAC_C Cuts at: 315 315 Size: 4190 BstXI CCAn_nnnn'nTGG Cuts at: 147 489 726 1170 147 Size: 342 237 444 3167 Fragments arranged by size: 3167 444 342 237 BstYI r'GATC_y Cuts at: 1 1087 1215 1682 1705 2040 2286 3298 3384 Size: 1086 128 467 23 335 246 1012 86 Cuts at: 3384 3396 3742 1 Size: 12 346 449 Fragments arranged by size: 1086 1012 467 449 346 335 246 128 86 23 12 BtgI C'CryG_G Cuts at: 482 482 Size: 4190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 802 2140 2321 2484 802 Size: 1338 181 163 2508 Fragments arranged by size: 2508 1338 181 163 BtsI GCAGTG_nn' Cuts at: 265 466 1433 265 Size: 201 967 3022 Fragments arranged by size: 3022 967 201 DraI TTT'AAA Cuts at: 1189 1189 Size: 4190 DraIII CAC_nnn'GTG Cuts at: 653 935 653 Size: 282 3908 DrdI GACnn_nn'nnGTC Cuts at: 1896 2754 1896 Size: 858 3332 EaeI y'GGCC_r Cuts at: 479 1775 1949 2340 2367 3712 479 Size: 1296 174 391 27 1345 957 Fragments arranged by size: 1345 1296 957 391 174 27 EagI C'GGCC_G Cuts at: 1775 1775 Size: 4190 EarI CTCTTCn'nnn_ Cuts at: 190 931 2213 2423 190 Size: 741 1282 210 1957 Fragments arranged by size: 1957 1282 741 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2398 2708 2854 4000 2398 Size: 310 146 1146 2588 Fragments arranged by size: 2588 1146 310 146 EcoICRI GAG'CTC Cuts at: 1202 1202 Size: 4190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 171 511 702 901 1028 1076 2015 2447 3194 Size: 340 191 199 127 48 939 432 747 Cuts at: 3194 3462 171 Size: 268 899 Fragments arranged by size: 939 899 747 432 340 268 199 191 127 48 EcoNI CCTnn'n_nnAGG Cuts at: 825 825 Size: 4190 EcoO109I rG'GnC_Cy Cuts at: 1001 1329 1001 Size: 328 3862 EcoRI G'AATT_C Cuts at: 1194 1194 Size: 4190 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2209 2209 Size: 4190 FspI TGC'GCA Cuts at: 775 1971 775 Size: 1196 2994 HaeII r_GCGC'y Cuts at: 581 733 770 913 1624 1872 2894 3873 581 Size: 152 37 143 711 248 1022 979 898 Fragments arranged by size: 1022 979 898 711 248 152 143 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 521 553 2032 2062 2064 2094 521 Size: 32 1479 30 2 30 2617 Fragments arranged by size: 2617 1479 32 30 30 2 HincII GTy'rAC Cuts at: 1229 1229 Size: 4190 KasI G'GCGC_C Cuts at: 766 1620 1868 766 Size: 854 248 3088 Fragments arranged by size: 3088 854 248 KpnI G_GTAC'C Cuts at: 1210 1210 Size: 4190 MfeI C'AATT_G Cuts at: 1055 1055 Size: 4190 MluI A'CGCG_T Cuts at: 3623 3623 Size: 4190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2861 3045 2861 Size: 184 4006 MscI TGG'CCA Cuts at: 481 1951 481 Size: 1470 2720 MslI CAynn'nnrTG Cuts at: 145 298 492 1168 2179 2306 145 Size: 153 194 676 1011 127 2029 Fragments arranged by size: 2029 1011 676 194 153 127 MspA1I CmG'CkG Cuts at: 128 276 807 1301 1615 1975 2988 3233 128 Size: 148 531 494 314 360 1013 245 1085 Fragments arranged by size: 1085 1013 531 494 360 314 245 148 NaeI GCC'GGC Cuts at: 2371 2371 Size: 4190 NarI GG'CG_CC Cuts at: 767 1621 1869 767 Size: 854 248 3088 Fragments arranged by size: 3088 854 248 NcoI C'CATG_G Cuts at: 482 482 Size: 4190 NgoMIV G'CCGG_C Cuts at: 2369 2369 Size: 4190 NspI r_CATG'y Cuts at: 297 1243 1743 2650 297 Size: 946 500 907 1837 Fragments arranged by size: 1837 946 907 500 PasI CC'CwG_GG Cuts at: 219 219 Size: 4190 PciI A'CATG_T Cuts at: 2646 2646 Size: 4190 PmeI GTTT'AAAC Cuts at: 1189 1189 Size: 4190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 127 159 3329 3361 127 Size: 32 3170 32 956 Fragments arranged by size: 3170 956 32 32 PpuMI rG'GwC_Cy Cuts at: 1001 1001 Size: 4190 PstI C_TGCA'G Cuts at: 806 1237 1922 806 Size: 431 685 3074 Fragments arranged by size: 3074 685 431 PvuI CG_AT'CG Cuts at: 104 104 Size: 4190 PvuII CAG'CTG Cuts at: 1615 1975 1615 Size: 360 3830 RsrII CG'GwC_CG Cuts at: 2385 2385 Size: 4190 SacI G_AGCT'C Cuts at: 1204 1204 Size: 4190 SalI G'TCGA_C Cuts at: 1227 1227 Size: 4190 SapI GCTCTTCn'nnn_ Cuts at: 190 931 2213 2423 190 Size: 741 1282 210 1957 Fragments arranged by size: 1957 1282 741 210 SbfI CC_TGCA'GG Cuts at: 1237 1237 Size: 4190 ScaI AGT'ACT Cuts at: 637 3707 637 Size: 3070 1120 SexAI A'CCwGG_T Cuts at: 649 649 Size: 4190 SfcI C'TryA_G Cuts at: 33 802 1233 1918 2911 3102 33 Size: 769 431 685 993 191 1121 Fragments arranged by size: 1121 993 769 685 431 191 SfoI GGC'GCC Cuts at: 768 1622 1870 768 Size: 854 248 3088 Fragments arranged by size: 3088 854 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4190 SgrAI Cr'CCGG_yG Cuts at: 794 794 Size: 4190 SmaI CCC'GGG Cuts at: 1212 1212 Size: 4190 SmlI C'TyrA_G Cuts at: 1345 2260 2752 3014 1345 Size: 915 492 262 2521 Fragments arranged by size: 2521 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4190 SphI G_CATG'C Cuts at: 1243 1743 1243 Size: 500 3690 StyI C'CwwG_G Cuts at: 482 507 1066 1324 1678 482 Size: 25 559 258 354 2994 Fragments arranged by size: 2994 559 354 258 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 273 1773 1906 2365 2574 273 Size: 1500 133 459 209 1889 Fragments arranged by size: 1889 1500 459 209 133 TatI w'GTAC_w Cuts at: 635 3705 635 Size: 3070 1120 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 458 490 802 834 3707 3739 458 Size: 32 312 32 2873 32 909 Fragments arranged by size: 2873 909 312 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1987 1987 Size: 4190 XbaI T'CTAG_A Cuts at: 1221 1221 Size: 4190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1685 1685 Size: 4190 XmaI C'CCGG_G Cuts at: 1210 1210 Size: 4190 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI ApaI AscI BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI