Restriction map for
pF1KB8979
(Circular) (Six-base) MAPSORT of: pF1KB8979.seq Check: 2672 from: 1 to: 4235
pF1KB8979 4235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 847 847
Size: 4235
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 365 1251 365
Size: 886 3349
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 453 820 2060 2492 3239 3507 341
Size: 112 367 1240 432 747 268 1069
Fragments arranged by size:
1240 1069 747 432 367 268 112
AflII C'TTAA_G
Cuts at: 381 381
Size: 4235
AflIII A'CryG_T
Cuts at: 635 745 1587 2691 3668 635
Size: 110 842 1104 977 1202
Fragments arranged by size:
1202 1104 977 842 110
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 749 749
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 133 1540 3107 133
Size: 1407 1567 1261
Fragments arranged by size:
1567 1407 1261
ApaLI G'TGCA_C
Cuts at: 3005 3005
Size: 4235
ApoI r'AATT_y
Cuts at: 243 1239 3588 3844 243
Size: 996 2349 256 634
Fragments arranged by size:
2349 996 634 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 1255 1255
Size: 4235
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 365 1251 1665 1913 1948 365
Size: 886 414 248 35 2652
Fragments arranged by size:
2652 886 414 248 35
BanII G_rGCy'C
Cuts at: 237 1249 2279 237
Size: 1012 1030 2193
Fragments arranged by size:
2193 1030 1012
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BfrBI ATG'CAT
Cuts at: 979 979
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 319 1860 1953 3009 319
Size: 1541 93 1056 1545
Fragments arranged by size:
1545 1541 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 591 1447 1528 1863 4007 591
Size: 856 81 335 2144 819
Fragments arranged by size:
2144 856 819 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2290 2782 3080 1411
Size: 879 492 298 2566
Fragments arranged by size:
2566 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3939 3939
Size: 4235
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 1666 1914 2616 4112 1666
Size: 248 702 1496 1789
Fragments arranged by size:
1789 1496 702 248
BsaWI w'CCGG_w
Cuts at: 965 1424 1945 2623 2897 3044 3455 965
Size: 459 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 459 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 883 913 883
Size: 30 4205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1009 1009
Size: 4235
BseYI C'CCAG_C
Cuts at: 1660 2418 2995 3491 1660
Size: 758 577 496 2404
Fragments arranged by size:
2404 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3031 104
Size: 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 237 1249 2027 2217 3009 237
Size: 1012 778 190 792 1463
Fragments arranged by size:
1463 1012 792 778 190
BsmI GAATG_Cn'
Cuts at: 907 907
Size: 4235
Bsp1286I G_dGCh'C
Cuts at: 237 319 1249 1860 1953 2027 2217 2279 3009
Size: 82 930 611 93 74 190 62 730
Cuts at: 3009 237
Size: 1463
Fragments arranged by size:
1463 930 730 611 190 93 82 74
62
BspEI T'CCGG_A
Cuts at: 965 3455 965
Size: 2490 1745
BspHI T'CATG_A
Cuts at: 3411 3411
Size: 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 408 847 1285 1499 1801 2182 408
Size: 439 438 214 302 381 2461
Fragments arranged by size:
2461 439 438 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2527 2581 4065 4230 50
Size: 2477 54 1484 165 55
Fragments arranged by size:
2477 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 515 541 2147 515
Size: 26 1606 2603
Fragments arranged by size:
2603 1606 26
BsrFI r'CCGG_y
Cuts at: 2233 2414 2623 2233
Size: 181 209 3845
Fragments arranged by size:
3845 209 181
BssSI C'ACGA_G
Cuts at: 2506 2864 2506
Size: 358 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstYI r'GATC_y
Cuts at: 1 478 1167 1260 1727 1750 2085 2331 3343
Size: 477 689 93 467 23 335 246 1012
Cuts at: 3343 3429 3441 3787 1
Size: 86 12 346 449
Fragments arranged by size:
1012 689 477 467 449 346 335 246
93 86 23 12
BtgI C'CryG_G
Cuts at: 359 359
Size: 4235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1478 1211
Size: 267 3968
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 437 512 686 1820 1994 2385 2412 3757 437
Size: 75 174 1134 174 391 27 1345 915
Fragments arranged by size:
1345 1134 915 391 174 174 75 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 248 2258 2468 248
Size: 2010 210 2015
Fragments arranged by size:
2015 2010 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 2753 2899 4045 2443
Size: 310 146 1146 2633
Fragments arranged by size:
2633 1146 310 146
EcoICRI GAG'CTC
Cuts at: 235 1247 235
Size: 1012 3223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 453 820 2060 2492 3239 3507 341
Size: 112 367 1240 432 747 268 1069
Fragments arranged by size:
1240 1069 747 432 367 268 112
EcoO109I rG'GnC_Cy
Cuts at: 597 714 1374 597
Size: 117 660 3458
Fragments arranged by size:
3458 660 117
EcoRI G'AATT_C
Cuts at: 1239 1239
Size: 4235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 225 2254 225
Size: 2029 2206
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 1669 1917 2939 3918 1669
Size: 248 1022 979 1986
Fragments arranged by size:
1986 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 578 856 888 2077 2107 2109 2139 546
Size: 32 278 32 1189 30 2 30 2642
Fragments arranged by size:
2642 1189 278 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1274 1274
Size: 4235
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 369 1255 369
Size: 886 3349
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 906 2906 3090 906
Size: 2000 184 2051
Fragments arranged by size:
2051 2000 184
MscI TGG'CCA
Cuts at: 514 688 1996 514
Size: 174 1308 2753
Fragments arranged by size:
2753 1308 174
MslI CAynn'nnrTG
Cuts at: 341 569 731 1027 2224 2351 341
Size: 228 162 296 1197 127 2225
Fragments arranged by size:
2225 1197 296 228 162 127
MspA1I CmG'CkG
Cuts at: 130 1346 1660 2020 3033 3278 130
Size: 1216 314 360 1013 245 1087
Fragments arranged by size:
1216 1087 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 4235
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NcoI C'CATG_G
Cuts at: 359 359
Size: 4235
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 4235
NsiI A_TGCA'T
Cuts at: 981 981
Size: 4235
NspI r_CATG'y
Cuts at: 639 749 1288 1788 2695 639
Size: 110 539 500 907 2179
Fragments arranged by size:
2179 907 539 500 110
PciI A'CATG_T
Cuts at: 635 745 2691 635
Size: 110 1946 2179
Fragments arranged by size:
2179 1946 110
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4203
PpuMI rG'GwC_Cy
Cuts at: 597 597
Size: 4235
PstI C_TGCA'G
Cuts at: 1282 1967 1282
Size: 685 3550
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 130 1660 2020 130
Size: 1530 360 2345
Fragments arranged by size:
2345 1530 360
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 237 1249 237
Size: 1012 3223
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 387 3752 387
Size: 3365 870
SfcI C'TryA_G
Cuts at: 33 1278 1963 2956 3147 33
Size: 1245 685 993 191 1121
Fragments arranged by size:
1245 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 381 1390 2305 2797 3059 381
Size: 1009 915 492 262 1557
Fragments arranged by size:
1557 1009 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
SspI AAT'ATT
Cuts at: 811 1062 811
Size: 251 3984
StyI C'CwwG_G
Cuts at: 272 359 737 902 1369 1723 272
Size: 87 378 165 467 354 2784
Fragments arranged by size:
2784 467 378 354 165 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1818 1951 2410 2619 1818
Size: 133 459 209 3434
Fragments arranged by size:
3434 459 209 133
TatI w'GTAC_w
Cuts at: 385 638 3750 385
Size: 253 3112 870
Fragments arranged by size:
3112 870 253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4203
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 411 1730 411
Size: 1319 2916
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI