Restriction map for
pF1KB8986
(Circular) (Six-base) MAPSORT of: pF1KB8986.seq Check: 3012 from: 1 to: 4274
pF1KB8986 4274 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4274
Acc65I G'GTAC_C
Cuts at: 584 1290 584
Size: 706 3568
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 822 2099 2531 3278 3546 822
Size: 1277 432 747 268 1550
Fragments arranged by size:
1550 1277 747 432 268
AflII C'TTAA_G
Cuts at: 970 970
Size: 4274
AflIII A'CryG_T
Cuts at: 1626 2730 3707 1626
Size: 1104 977 2193
Fragments arranged by size:
2193 1104 977
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AleI CACnn'nnGTG
Cuts at: 1241 1241
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 855 1579 3146 855
Size: 724 1567 1983
Fragments arranged by size:
1983 1567 724
ApaLI G'TGCA_C
Cuts at: 3044 3044
Size: 4274
ApoI r'AATT_y
Cuts at: 348 511 789 1278 3627 3883 348
Size: 163 278 489 2349 256 739
Fragments arranged by size:
2349 739 489 278 256 163
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 742 1294 742
Size: 552 3722
AvrII C'CTAG_G
Cuts at: 654 654
Size: 4274
BamHI G'GATC_C
Cuts at: 1299 1299
Size: 4274
BanI G'GyrC_C
Cuts at: 584 1290 1704 1952 1987 584
Size: 706 414 248 35 2871
Fragments arranged by size:
2871 706 414 248 35
BanII G_rGCy'C
Cuts at: 163 1288 2318 163
Size: 1125 1030 2119
Fragments arranged by size:
2119 1125 1030
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4026
BbvCI CC'TCA_GC
Cuts at: 257 257
Size: 4274
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2933 4274 2172
Size: 761 1341 2172
Fragments arranged by size:
2172 1341 761
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BfrBI ATG'CAT
Cuts at: 1084 1255 1084
Size: 171 4103
BglI GCCn_nnn'nGGC
Cuts at: 193 193
Size: 4274
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 1386 1386
Size: 4274
Bme1580I G_kGCm'C
Cuts at: 1899 1992 3048 1899
Size: 93 1056 3125
Fragments arranged by size:
3125 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 687 986 1486 1567 1902 4046 687
Size: 299 500 81 335 2144 915
Fragments arranged by size:
2144 915 500 335 299 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 736 982 142
Size: 594 246 3434
Fragments arranged by size:
3434 594 246
Bpu10I CC'TnA_GC
Cuts at: 257 257
Size: 4274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2329 2821 3119 1450
Size: 879 492 298 2605
Fragments arranged by size:
2605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3978 3978
Size: 4274
BsaAI yAC'GTr
Cuts at: 1627 1627
Size: 4274
BsaBI GATnn'nnATC
Cuts at: 6 1788 3825 6
Size: 1782 2037 455
Fragments arranged by size:
2037 1782 455
BsaHI Gr'CG_yC
Cuts at: 641 659 1705 1953 2655 4151 641
Size: 18 1046 248 702 1496 764
Fragments arranged by size:
1496 1046 764 702 248 18
BsaWI w'CCGG_w
Cuts at: 623 1463 1984 2662 2936 3083 3494 623
Size: 840 521 678 274 147 411 1403
Fragments arranged by size:
1403 840 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1167 1167
Size: 4274
BseYI C'CCAG_C
Cuts at: 1090 1699 2457 3034 3530 1090
Size: 609 758 577 496 1834
Fragments arranged by size:
1834 758 609 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 652
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3070 104
Size: 1712 46 1208 1308
Fragments arranged by size:
1712 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 163 773 1288 2066 2256 3048 163
Size: 610 515 778 190 792 1389
Fragments arranged by size:
1389 792 778 610 515 190
BsmI GAATG_Cn'
Cuts at: 730 730
Size: 4274
BsmBI CGTCTCn'nnnn_
Cuts at: 958 958
Size: 4274
Bsp1286I G_dGCh'C
Cuts at: 163 773 1288 1899 1992 2066 2256 2318 3048
Size: 610 515 611 93 74 190 62 730
Cuts at: 3048 163
Size: 1389
Fragments arranged by size:
1389 730 611 610 515 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3494 3494
Size: 4274
BspHI T'CATG_A
Cuts at: 3450 3450
Size: 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1324 1538 1840 2221 1324
Size: 214 302 381 3377
Fragments arranged by size:
3377 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2566 2620 4104 4269 50
Size: 2516 54 1484 165 55
Fragments arranged by size:
2516 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 783 1255 2186 783
Size: 472 931 2871
Fragments arranged by size:
2871 931 472
BsrFI r'CCGG_y
Cuts at: 2272 2453 2662 2272
Size: 181 209 3884
Fragments arranged by size:
3884 209 181
BssSI C'ACGA_G
Cuts at: 1239 2545 2903 1239
Size: 1306 358 2610
Fragments arranged by size:
2610 1306 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 1651
Size: 4274
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstXI CCAn_nnnn'nTGG
Cuts at: 339 600 681 1126 339
Size: 261 81 445 3487
Fragments arranged by size:
3487 445 261 81
BstYI r'GATC_y
Cuts at: 1 1011 1299 1766 1789 2124 2370 3382 3468
Size: 1010 288 467 23 335 246 1012 86
Cuts at: 3468 3480 3826 1
Size: 12 346 449
Fragments arranged by size:
1012 1010 467 449 346 335 288 246
86 23 12
BtgI C'CryG_G
Cuts at: 203 203
Size: 4274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 1517 1517
Size: 4274
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 283 1980 2838 283
Size: 1697 858 1719
Fragments arranged by size:
1719 1697 858
EaeI y'GGCC_r
Cuts at: 185 590 602 1859 2033 2424 2451 3796 185
Size: 405 12 1257 174 391 27 1345 663
Fragments arranged by size:
1345 1257 663 405 391 174 27 12
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4274
EarI CTCTTCn'nnn_
Cuts at: 151 934 2297 2507 151
Size: 783 1363 210 1918
Fragments arranged by size:
1918 1363 783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2482 2792 2938 4084 2482
Size: 310 146 1146 2672
Fragments arranged by size:
2672 1146 310 146
EcoICRI GAG'CTC
Cuts at: 161 1286 161
Size: 1125 3149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 736 822 982 2099 2531 3278 3546 142
Size: 594 86 160 1117 432 747 268 870
Fragments arranged by size:
1117 870 747 594 432 268 160 86
EcoO109I rG'GnC_Cy
Cuts at: 194 489 747 1413 194
Size: 295 258 666 3055
Fragments arranged by size:
3055 666 295 258
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 4274
FspI TGC'GCA
Cuts at: 2055 2055
Size: 4274
HaeII r_GCGC'y
Cuts at: 1158 1708 1956 2978 3957 1158
Size: 550 248 1022 979 1475
Fragments arranged by size:
1475 1022 979 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 996 1028 1162 1194 2116 2146 2148 2178 996
Size: 32 134 32 922 30 2 30 3092
Fragments arranged by size:
3092 922 134 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1313 1313
Size: 4274
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4026
KpnI G_GTAC'C
Cuts at: 588 1294 588
Size: 706 3568
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3129 2945
Size: 184 4090
MscI TGG'CCA
Cuts at: 187 592 2035 187
Size: 405 1443 2426
Fragments arranged by size:
2426 1443 405
MslI CAynn'nnrTG
Cuts at: 679 1124 1208 1241 2263 2390 679
Size: 445 84 33 1022 127 2563
Fragments arranged by size:
2563 1022 445 127 84 33
MspA1I CmG'CkG
Cuts at: 1385 1699 2059 3072 3317 1385
Size: 314 360 1013 245 2342
Fragments arranged by size:
2342 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4274
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4026
NcoI C'CATG_G
Cuts at: 203 203
Size: 4274
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4274
NsiI A_TGCA'T
Cuts at: 1086 1257 1086
Size: 171 4103
NspI r_CATG'y
Cuts at: 1327 1827 2734 1327
Size: 500 907 2867
Fragments arranged by size:
2867 907 500
PciI A'CATG_T
Cuts at: 2730 2730
Size: 4274
PfoI T'CCnGG_A
Cuts at: 752 959 752
Size: 207 4067
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PpuMI rG'GwC_Cy
Cuts at: 747 747
Size: 4274
PstI C_TGCA'G
Cuts at: 1321 2006 1321
Size: 685 3589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 1699 2059 1699
Size: 360 3914
RsrII CG'GwC_CG
Cuts at: 626 2469 626
Size: 1843 2431
SacI G_AGCT'C
Cuts at: 163 1288 163
Size: 1125 3149
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 151 2297 2507 151
Size: 2146 210 1918
Fragments arranged by size:
2146 1918 210
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 1317 2002 2995 3186 33
Size: 1284 685 993 191 1121
Fragments arranged by size:
1284 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 193 193
Size: 4274
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4026
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4274
SmlI C'TyrA_G
Cuts at: 970 1429 2344 2836 3098 970
Size: 459 915 492 262 2146
Fragments arranged by size:
2146 915 492 459 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 1327 1827 1327
Size: 500 3774
StuI AGG'CCT
Cuts at: 262 262
Size: 4274
StyI C'CwwG_G
Cuts at: 203 654 729 1408 1762 203
Size: 451 75 679 354 2715
Fragments arranged by size:
2715 679 451 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 1857 1990 2449 2658 164
Size: 1693 133 459 209 1780
Fragments arranged by size:
1780 1693 459 209 133
TatI w'GTAC_w
Cuts at: 3789 3789
Size: 4274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4242
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 1243 1769 339
Size: 904 526 2844
Fragments arranged by size:
2844 904 526
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4274
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
BaeI BbsI BcgI BmgBI BmtI BsaXI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI