Restriction map for
pF1KB8990
(Circular) (Six-base) MAPSORT of: pF1KB8990.seq Check: 888 from: 1 to: 4286
pF1KB8990 4286 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 843 843
Size: 4286
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2543 3290 3558 2111
Size: 432 747 268 2839
Fragments arranged by size:
2839 747 432 268
AfeI AGC'GCT
Cuts at: 1120 1120
Size: 4286
AflIII A'CryG_T
Cuts at: 1638 2742 3719 1638
Size: 1104 977 2205
Fragments arranged by size:
2205 1104 977
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 542 858 1591 3158 542
Size: 316 733 1567 1670
Fragments arranged by size:
1670 1567 733 316
ApaI G_GGCC'C
Cuts at: 215 215
Size: 4286
ApaLI G'TGCA_C
Cuts at: 3056 3056
Size: 4286
ApoI r'AATT_y
Cuts at: 1290 3639 3895 1290
Size: 2349 256 1681
Fragments arranged by size:
2349 1681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 1306 1306
Size: 4286
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 1302 1716 1964 1999 1302
Size: 414 248 35 3589
Fragments arranged by size:
3589 414 248 35
BanII G_rGCy'C
Cuts at: 215 676 1300 2330 215
Size: 461 624 1030 2171
Fragments arranged by size:
2171 1030 624 461
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 342 342
Size: 4286
BbvCI CC'TCA_GC
Cuts at: 343 343
Size: 4286
BciVI GTATCCnnnnn_n'
Cuts at: 978 2184 2945 4286 978
Size: 1206 761 1341 978
Fragments arranged by size:
1341 1206 978 761
BclI T'GATC_A
Cuts at: 1806 1806
Size: 4286
BglII A'GATC_T
Cuts at: 386 1801 3838 386
Size: 1415 2037 834
Fragments arranged by size:
2037 1415 834
BlpI GC'TnA_GC
Cuts at: 1398 1398
Size: 4286
Bme1580I G_kGCm'C
Cuts at: 164 215 1213 1911 2004 3060 164
Size: 51 998 698 93 1056 1390
Fragments arranged by size:
1390 1056 998 698 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 1272 1498 1579 1914 4058 1272
Size: 226 81 335 2144 1500
Fragments arranged by size:
2144 1500 335 226 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 996
Size: 4286
Bpu10I CC'TnA_GC
Cuts at: 343 670 343
Size: 327 3959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2341 2833 3131 1462
Size: 879 492 298 2617
Fragments arranged by size:
2617 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3990 3990
Size: 4286
BsaAI yAC'GTr
Cuts at: 1104 1639 1104
Size: 535 3751
BsaBI GATnn'nnATC
Cuts at: 6 844 1800 3837 6
Size: 838 956 2037 455
Fragments arranged by size:
2037 956 838 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 692 1475 1996 2674 2948 3095 3506 692
Size: 783 521 678 274 147 411 1472
Fragments arranged by size:
1472 783 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 918 918
Size: 4286
BseYI C'CCAG_C
Cuts at: 264 623 1711 2469 3046 3542 264
Size: 359 1088 758 577 496 1008
Fragments arranged by size:
1088 1008 758 577 496 359
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 357 819 343
Size: 14 462 3810
Fragments arranged by size:
3810 462 14
BsiEI CG_ry'CG
Cuts at: 104 570 1828 1874 3082 104
Size: 466 1258 46 1208 1308
Fragments arranged by size:
1308 1258 1208 466 46
BsiHKAI G_wGCw'C
Cuts at: 676 1300 2078 2268 3060 676
Size: 624 778 190 792 1902
Fragments arranged by size:
1902 792 778 624 190
BsmI GAATG_Cn'
Cuts at: 513 513
Size: 4286
BsmBI CGTCTCn'nnnn_
Cuts at: 481 481
Size: 4286
Bsp1286I G_dGCh'C
Cuts at: 164 215 676 1213 1300 1911 2004 2078 2268
Size: 51 461 537 87 611 93 74 190
Cuts at: 2268 2330 3060 164
Size: 62 730 1390
Fragments arranged by size:
1390 730 611 537 461 190 93 87
74 62 51
BspEI T'CCGG_A
Cuts at: 692 3506 692
Size: 2814 1472
BspHI T'CATG_A
Cuts at: 3462 3462
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 843 1166 1336 1550 1852 2233 843
Size: 323 170 214 302 381 2896
Fragments arranged by size:
2896 381 323 302 214 170
BsrBI CCG'CTC
Cuts at: 50 2578 2632 4116 4281 50
Size: 2528 54 1484 165 55
Fragments arranged by size:
2528 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 2284 2465 2674 2284
Size: 181 209 3896
Fragments arranged by size:
3896 209 181
BsrGI T'GTAC_A
Cuts at: 530 530
Size: 4286
BssSI C'ACGA_G
Cuts at: 2557 2915 2557
Size: 358 3928
BstAPI GCAn_nnn'nTGC
Cuts at: 849 858 1082 1663 849
Size: 9 224 581 3472
Fragments arranged by size:
3472 581 224 9
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 600 1194 1233 600
Size: 594 39 3653
Fragments arranged by size:
3653 594 39
BstYI r'GATC_y
Cuts at: 1 386 890 1311 1778 1801 2136 2382 3394
Size: 385 504 421 467 23 335 246 1012
Cuts at: 3394 3480 3492 3838 1
Size: 86 12 346 449
Fragments arranged by size:
1012 504 467 449 421 385 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 167 599 911 1205 1232 167
Size: 432 312 294 27 3221
Fragments arranged by size:
3221 432 312 294 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 728 979 2236 2417 2580 728
Size: 251 1257 181 163 2434
Fragments arranged by size:
2434 1257 251 181 163
BtsI GCAGTG_nn'
Cuts at: 1213 1529 1213
Size: 316 3970
ClaI AT'CG_AT
Cuts at: 1037 1037
Size: 4286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 241 276 241
Size: 35 4251
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 2850 1992
Size: 858 3428
EaeI y'GGCC_r
Cuts at: 317 404 1871 2045 2436 2463 3808 317
Size: 87 1467 174 391 27 1345 795
Fragments arranged by size:
1467 1345 795 391 174 87 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2494 2804 2950 4096 2494
Size: 310 146 1146 2684
Fragments arranged by size:
2684 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 1298 674
Size: 624 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 2111 2543 3290 3558 996
Size: 1115 432 747 268 1724
Fragments arranged by size:
1724 1115 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 211 227 1425 211
Size: 16 1198 3072
Fragments arranged by size:
3072 1198 16
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
EcoRV GAT'ATC
Cuts at: 128 128
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 4286
FspI TGC'GCA
Cuts at: 2067 2067
Size: 4286
HaeII r_GCGC'y
Cuts at: 185 1122 1720 1968 2990 3969 185
Size: 937 598 248 1022 979 502
Fragments arranged by size:
1022 979 937 598 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2128 2158 2160 2190 2128
Size: 30 2 30 4224
Fragments arranged by size:
4224 30 30 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 2957 3141 485
Size: 2472 184 1630
Fragments arranged by size:
2472 1630 184
MscI TGG'CCA
Cuts at: 319 2047 319
Size: 1728 2558
MslI CAynn'nnrTG
Cuts at: 598 685 781 853 1192 1231 2275 2402 598
Size: 87 96 72 339 39 1044 127 2482
Fragments arranged by size:
2482 1044 339 127 96 87 72 39
MspA1I CmG'CkG
Cuts at: 371 1016 1082 1397 1711 2071 3084 3329 371
Size: 645 66 315 314 360 1013 245 1328
Fragments arranged by size:
1328 1013 645 360 315 314 245 66
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 599 1205 599
Size: 606 3680
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 192 786 1339 1839 2746 192
Size: 594 553 500 907 1732
Fragments arranged by size:
1732 907 594 553 500
PciI A'CATG_T
Cuts at: 2742 2742
Size: 4286
PfoI T'CCnGG_A
Cuts at: 980 980
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4254
PpuMI rG'GwC_Cy
Cuts at: 227 227
Size: 4286
PspOMI G'GGCC_C
Cuts at: 211 211
Size: 4286
PstI C_TGCA'G
Cuts at: 1177 1259 1333 2018 1177
Size: 82 74 685 3445
Fragments arranged by size:
3445 685 82 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 371 1016 1082 1711 2071 371
Size: 645 66 629 360 2586
Fragments arranged by size:
2586 645 629 360 66
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 676 1300 676
Size: 624 3662
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 636 1071 1173 1255 1329 2014 3007 3198
Size: 603 435 102 82 74 685 993 191
Cuts at: 3198 33
Size: 1121
Fragments arranged by size:
1121 993 685 603 435 191 102 82
74
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 1441 2356 2848 3110 1441
Size: 915 492 262 2617
Fragments arranged by size:
2617 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
StyI C'CwwG_G
Cuts at: 599 1205 1420 1774 599
Size: 606 215 354 3111
Fragments arranged by size:
3111 606 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 291 693 1869 2002 2461 2670 291
Size: 402 1176 133 459 209 1907
Fragments arranged by size:
1907 1176 459 402 209 133
TatI w'GTAC_w
Cuts at: 530 707 3801 530
Size: 177 3094 1015
Fragments arranged by size:
3094 1015 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4254
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 761 1781 761
Size: 1020 3266
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
XmnI GAAnn'nnTTC
Cuts at: 1187 1187
Size: 4286
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BssHII BstEII BstZ17I Bsu36I DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI