Restriction map for
pF1KB8992
(Circular) (Six-base) MAPSORT of: pF1KB8992.seq Check: 7331 from: 1 to: 3716
pF1KB8992 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
AflII C'TTAA_G
Cuts at: 693 693
Size: 3716
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 487 487
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 147 359 498 1021 2588 147
Size: 212 139 523 1567 1275
Fragments arranged by size:
1567 1275 523 212 139
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 736 736
Size: 3716
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 231 732 1146 1394 1429 231
Size: 501 414 248 35 2518
Fragments arranged by size:
2518 501 414 248 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 1231 3268 1231
Size: 2037 1679
BlpI GC'TnA_GC
Cuts at: 268 828 268
Size: 560 3156
Bme1580I G_kGCm'C
Cuts at: 236 466 1341 1434 2490 236
Size: 230 875 93 1056 1462
Fragments arranged by size:
1462 1056 875 230 93
BmgBI CAC'GTC
Cuts at: 312 399 576 312
Size: 87 177 3452
Fragments arranged by size:
3452 177 87
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 430
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 649 892 1771 2263 2561 190
Size: 459 243 879 492 298 1345
Fragments arranged by size:
1345 879 492 459 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 381 1069 381
Size: 688 3028
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 111 427 111
Size: 316 3400
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 415 529 345
Size: 70 114 3532
Fragments arranged by size:
3532 114 70
BsiEI CG_ry'CG
Cuts at: 104 179 337 1258 1304 2512 104
Size: 75 158 921 46 1208 1308
Fragments arranged by size:
1308 1208 921 158 75 46
BsiHKAI G_wGCw'C
Cuts at: 197 730 1508 1698 2490 197
Size: 533 778 190 792 1423
Fragments arranged by size:
1423 792 778 533 190
Bsp1286I G_dGCh'C
Cuts at: 197 236 466 730 1341 1434 1508 1698 1760
Size: 39 230 264 611 93 74 190 62
Cuts at: 1760 2490 197
Size: 730 1423
Fragments arranged by size:
1423 730 611 264 230 190 93 74
62 39
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 766 980 1282 1663 506
Size: 260 214 302 381 2559
Fragments arranged by size:
2559 381 302 260 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 178 200 404 513 582 1714 1895 2104 178
Size: 22 204 109 69 1132 181 209 1790
Fragments arranged by size:
1790 1132 209 204 181 109 69 22
BssSI C'ACGA_G
Cuts at: 547 1987 2345 547
Size: 1440 358 1918
Fragments arranged by size:
1918 1440 358
BstAPI GCAn_nnn'nTGC
Cuts at: 296 498 1093 296
Size: 202 595 2919
Fragments arranged by size:
2919 595 202
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstEII G'GTnAC_C
Cuts at: 438 438
Size: 3716
BstYI r'GATC_y
Cuts at: 1 741 1208 1231 1566 1812 2824 2910 2922
Size: 740 467 23 335 246 1012 86 12
Cuts at: 2922 3268 1
Size: 346 449
Fragments arranged by size:
1012 740 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 188 107
Size: 81 3635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 465 1666 1847 2010 465
Size: 1201 181 163 2171
Fragments arranged by size:
2171 1201 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DraIII CAC_nnn'GTG
Cuts at: 438 438
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 176 281 318 334 560 1301 1475 1866 1893
Size: 105 37 16 226 741 174 391 27
Cuts at: 1893 3238 176
Size: 1345 654
Fragments arranged by size:
1345 741 654 391 226 174 105 37
27 16
EagI C'GGCC_G
Cuts at: 176 334 1301 176
Size: 158 967 2591
Fragments arranged by size:
2591 967 158
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1541 1973 2720 2988 430
Size: 1111 432 747 268 1158
Fragments arranged by size:
1158 1111 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 613 613
Size: 3716
EcoO109I rG'GnC_Cy
Cuts at: 495 606 855 495
Size: 111 249 3356
Fragments arranged by size:
3356 249 111
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 544 1150 1398 2420 3399 544
Size: 606 248 1022 979 861
Fragments arranged by size:
1022 979 861 606 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 379 1705 1832 379
Size: 1326 127 2263
Fragments arranged by size:
2263 1326 127
MspA1I CmG'CkG
Cuts at: 296 339 359 471 537 827 1141 1501 2514
Size: 43 20 112 66 290 314 360 1013
Cuts at: 2514 2759 296
Size: 245 1253
Fragments arranged by size:
1253 1013 360 314 290 245 112 66
43 20
NaeI GCC'GGC
Cuts at: 180 584 1897 180
Size: 404 1313 1999
Fragments arranged by size:
1999 1313 404
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 107 107
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 178 582 1895 178
Size: 404 1313 1999
Fragments arranged by size:
1999 1313 404
NotI GC'GGCC_GC
Cuts at: 334 334
Size: 3716
NspI r_CATG'y
Cuts at: 769 1269 2176 769
Size: 500 907 2309
Fragments arranged by size:
2309 907 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PpuMI rG'GwC_Cy
Cuts at: 495 495
Size: 3716
PstI C_TGCA'G
Cuts at: 763 1448 763
Size: 685 3031
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 296 537 1141 1501 296
Size: 241 604 360 2511
Fragments arranged by size:
2511 604 360 241
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 351 759 1444 2437 2628 33
Size: 318 408 685 993 191 1121
Fragments arranged by size:
1121 993 685 408 318 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 205 664 693 871 1786 2278 2540 205
Size: 459 29 178 915 492 262 1381
Fragments arranged by size:
1381 915 492 459 262 178 29
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 107 284 850 1204 107
Size: 177 566 354 2619
Fragments arranged by size:
2619 566 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI