(Circular) (Six-base) MAPSORT of: pF1KB8993.seq Check: 6246 from: 1 to: 4316 pF1KB8993 4316 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 1354 1354 Size: 4316 Acc65I G'GTAC_C Cuts at: 1332 1332 Size: 4316 AclI AA'CG_TT Cuts at: 1310 4156 1310 Size: 2846 1470 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 107 149 821 2141 2573 3320 3588 107 Size: 42 672 1320 432 747 268 835 Fragments arranged by size: 1320 835 747 672 432 268 42 AflII C'TTAA_G Cuts at: 612 612 Size: 4316 AflIII A'CryG_T Cuts at: 114 1668 2772 3749 114 Size: 1554 1104 977 681 Fragments arranged by size: 1554 1104 977 681 AgeI A'CCGG_T Cuts at: 2704 2704 Size: 4316 AhdI GACnn_n'nnGTC Cuts at: 225 225 Size: 4316 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 188 220 188 Size: 32 4284 AlwNI CAG_nnn'CTG Cuts at: 1621 3188 1621 Size: 1567 2749 ApaLI G'TGCA_C Cuts at: 3086 3086 Size: 4316 ApoI r'AATT_y Cuts at: 452 542 552 642 837 888 1053 1320 3669 Size: 90 10 90 195 51 165 267 2349 Cuts at: 3669 3925 452 Size: 256 843 Fragments arranged by size: 2349 843 267 256 195 165 90 90 51 10 AseI AT'TA_AT Cuts at: 20 428 20 Size: 408 3908 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4316 AvaI C'yCGr_G Cuts at: 1336 1336 Size: 4316 BamHI G'GATC_C Cuts at: 268 1341 268 Size: 1073 3243 BanI G'GyrC_C Cuts at: 1332 1746 1994 2029 1332 Size: 414 248 35 3619 Fragments arranged by size: 3619 414 248 35 BanII G_rGCy'C Cuts at: 1330 2360 1330 Size: 1030 3286 BbeI G_GCGC'C Cuts at: 1750 1998 1750 Size: 248 4068 BciVI GTATCCnnnnn_n' Cuts at: 2214 2975 4316 2214 Size: 761 1341 2214 Fragments arranged by size: 2214 1341 761 BclI T'GATC_A Cuts at: 1836 1836 Size: 4316 BglII A'GATC_T Cuts at: 562 1831 3868 562 Size: 1269 2037 1010 Fragments arranged by size: 2037 1269 1010 BlpI GC'TnA_GC Cuts at: 1428 1428 Size: 4316 Bme1580I G_kGCm'C Cuts at: 1941 2034 3090 1941 Size: 93 1056 3167 Fragments arranged by size: 3167 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1528 1609 1944 4088 1528 Size: 81 335 2144 1756 Fragments arranged by size: 2144 1756 335 81 BmtI G_CTAG'C Cuts at: 1267 1267 Size: 4316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2683 2683 Size: 4316 Bpu10I CC'TnA_GC Cuts at: 792 792 Size: 4316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 116 1492 2371 2863 3161 116 Size: 1376 879 492 298 1271 Fragments arranged by size: 1376 1271 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 188 4020 188 Size: 3832 484 BsaAI yAC'GTr Cuts at: 1669 1669 Size: 4316 BsaBI GATnn'nnATC Cuts at: 6 1830 3867 6 Size: 1824 2037 455 Fragments arranged by size: 2037 1824 455 BsaHI Gr'CG_yC Cuts at: 1747 1995 2697 4193 1747 Size: 248 702 1496 1870 Fragments arranged by size: 1870 1496 702 248 BsaWI w'CCGG_w Cuts at: 1505 2026 2704 2978 3125 3536 1505 Size: 521 678 274 147 411 2285 Fragments arranged by size: 2285 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 168 198 453 483 168 Size: 30 255 30 4001 Fragments arranged by size: 4001 255 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 911 911 Size: 4316 BseYI C'CCAG_C Cuts at: 1741 2499 3076 3572 1741 Size: 758 577 496 2485 Fragments arranged by size: 2485 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1232 1858 1904 3112 104 Size: 1128 626 46 1208 1308 Fragments arranged by size: 1308 1208 1128 626 46 BsiHKAI G_wGCw'C Cuts at: 1330 2108 2298 3090 1330 Size: 778 190 792 2556 Fragments arranged by size: 2556 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1330 1941 2034 2108 2298 2360 3090 1330 Size: 611 93 74 190 62 730 2556 Fragments arranged by size: 2556 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3536 3536 Size: 4316 BspHI T'CATG_A Cuts at: 3492 3492 Size: 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1366 1580 1882 2263 1366 Size: 214 302 381 3419 Fragments arranged by size: 3419 381 302 214 BsrBI CCG'CTC Cuts at: 50 2608 2662 4146 4311 50 Size: 2558 54 1484 165 55 Fragments arranged by size: 2558 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2228 2228 Size: 4316 BsrFI r'CCGG_y Cuts at: 2314 2495 2704 2314 Size: 181 209 3926 Fragments arranged by size: 3926 209 181 BssSI C'ACGA_G Cuts at: 2587 2945 2587 Size: 358 3958 BstAPI GCAn_nnn'nTGC Cuts at: 1693 1693 Size: 4316 BstBI TT'CG_AA Cuts at: 2677 2677 Size: 4316 BstYI r'GATC_y Cuts at: 1 268 562 1341 1808 1831 2166 2412 3424 Size: 267 294 779 467 23 335 246 1012 Cuts at: 3424 3510 3522 3868 1 Size: 86 12 346 449 Fragments arranged by size: 1012 779 467 449 346 335 294 267 246 86 23 12 BtgI C'CryG_G Cuts at: 107 1210 107 Size: 1103 3213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2266 2447 2610 2266 Size: 181 163 3972 Fragments arranged by size: 3972 181 163 BtsI GCAGTG_nn' Cuts at: 1559 1559 Size: 4316 DraI TTT'AAA Cuts at: 842 986 1315 842 Size: 144 329 3843 Fragments arranged by size: 3843 329 144 DraIII CAC_nnn'GTG Cuts at: 1050 1050 Size: 4316 DrdI GACnn_nn'nnGTC Cuts at: 2022 2880 2022 Size: 858 3458 EaeI y'GGCC_r Cuts at: 1901 2075 2466 2493 3838 1901 Size: 174 391 27 1345 2379 Fragments arranged by size: 2379 1345 391 174 27 EagI C'GGCC_G Cuts at: 1901 1901 Size: 4316 EarI CTCTTCn'nnn_ Cuts at: 445 587 676 1274 2339 2549 445 Size: 142 89 598 1065 210 2212 Fragments arranged by size: 2212 1065 598 210 142 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2524 2834 2980 4126 2524 Size: 310 146 1146 2714 Fragments arranged by size: 2714 1146 310 146 EcoICRI GAG'CTC Cuts at: 1328 1328 Size: 4316 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 107 149 821 2141 2573 3320 3588 107 Size: 42 672 1320 432 747 268 835 Fragments arranged by size: 1320 835 747 672 432 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 177 177 Size: 4316 EcoO109I rG'GnC_Cy Cuts at: 1455 1455 Size: 4316 EcoRI G'AATT_C Cuts at: 1320 1320 Size: 4316 EcoRV GAT'ATC Cuts at: 1153 1153 Size: 4316 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 881 2335 881 Size: 1454 2862 FspI TGC'GCA Cuts at: 2097 2097 Size: 4316 HaeII r_GCGC'y Cuts at: 1750 1998 3020 3999 1750 Size: 248 1022 979 2067 Fragments arranged by size: 2067 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 211 243 586 618 2158 2188 2190 2220 211 Size: 32 343 32 1540 30 2 30 2307 Fragments arranged by size: 2307 1540 343 32 32 30 30 2 HincII GTy'rAC Cuts at: 995 1355 995 Size: 360 3956 KasI G'GCGC_C Cuts at: 1746 1994 1746 Size: 248 4068 KpnI G_GTAC'C Cuts at: 1336 1336 Size: 4316 MluI A'CGCG_T Cuts at: 3749 3749 Size: 4316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 924 2987 3171 924 Size: 2063 184 2069 Fragments arranged by size: 2069 2063 184 MscI TGG'CCA Cuts at: 2077 2077 Size: 4316 MslI CAynn'nnrTG Cuts at: 2305 2432 2305 Size: 127 4189 MspA1I CmG'CkG Cuts at: 1246 1427 1741 2101 3114 3359 1246 Size: 181 314 360 1013 245 2203 Fragments arranged by size: 2203 1013 360 314 245 181 NaeI GCC'GGC Cuts at: 2497 2497 Size: 4316 NarI GG'CG_CC Cuts at: 1747 1995 1747 Size: 248 4068 NcoI C'CATG_G Cuts at: 107 107 Size: 4316 NdeI CA'TA_TG Cuts at: 1007 1007 Size: 4316 NgoMIV G'CCGG_C Cuts at: 2495 2495 Size: 4316 NheI G'CTAG_C Cuts at: 1263 1263 Size: 4316 NspI r_CATG'y Cuts at: 118 247 979 1032 1194 1369 1869 2776 118 Size: 129 732 53 162 175 500 907 1658 Fragments arranged by size: 1658 907 732 500 175 162 129 53 PasI CC'CwG_GG Cuts at: 208 208 Size: 4316 PciI A'CATG_T Cuts at: 114 2772 114 Size: 2658 1658 PmeI GTTT'AAAC Cuts at: 1315 1315 Size: 4316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 3455 Size: 32 4284 PsiI TTA'TAA Cuts at: 635 635 Size: 4316 PstI C_TGCA'G Cuts at: 1363 2048 1363 Size: 685 3631 PvuI CG_AT'CG Cuts at: 104 1232 104 Size: 1128 3188 PvuII CAG'CTG Cuts at: 1246 1741 2101 1246 Size: 495 360 3461 Fragments arranged by size: 3461 495 360 RsrII CG'GwC_CG Cuts at: 2511 2511 Size: 4316 SacI G_AGCT'C Cuts at: 1330 1330 Size: 4316 SalI G'TCGA_C Cuts at: 1353 1353 Size: 4316 SapI GCTCTTCn'nnn_ Cuts at: 1274 2339 2549 1274 Size: 1065 210 3041 Fragments arranged by size: 3041 1065 210 SbfI CC_TGCA'GG Cuts at: 1363 1363 Size: 4316 ScaI AGT'ACT Cuts at: 3833 3833 Size: 4316 SfcI C'TryA_G Cuts at: 33 1359 2044 3037 3228 33 Size: 1326 685 993 191 1121 Fragments arranged by size: 1326 1121 993 685 191 SfoI GGC'GCC Cuts at: 1748 1996 1748 Size: 248 4068 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4316 SmaI CCC'GGG Cuts at: 1338 1338 Size: 4316 SmlI C'TyrA_G Cuts at: 131 612 1471 2386 2878 3140 131 Size: 481 859 915 492 262 1307 Fragments arranged by size: 1307 915 859 492 481 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4316 SphI G_CATG'C Cuts at: 1369 1869 1369 Size: 500 3816 SspI AAT'ATT Cuts at: 482 482 Size: 4316 StyI C'CwwG_G Cuts at: 107 1450 1804 107 Size: 1343 354 2619 Fragments arranged by size: 2619 1343 354 SwaI ATTT'AAAT Cuts at: 842 842 Size: 4316 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1899 2032 2491 2700 1899 Size: 133 459 209 3515 Fragments arranged by size: 3515 459 209 133 TatI w'GTAC_w Cuts at: 3831 3831 Size: 4316 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 168 200 1137 1169 3833 3865 168 Size: 32 937 32 2664 32 619 Fragments arranged by size: 2664 937 619 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2113 2113 Size: 4316 XbaI T'CTAG_A Cuts at: 1347 1347 Size: 4316 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1811 1811 Size: 4316 XmaI C'CCGG_G Cuts at: 1336 1336 Size: 4316 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AleI AlfI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BpmI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI XhoI XmnI ZraI