(Circular) (Six-base) MAPSORT of: pF1KB8994.seq Check: 2755 from: 1 to: 4100 pF1KB8994 4100 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 261 261 Size: 4100 AccI GT'mk_AC Cuts at: 368 1079 1138 368 Size: 711 59 3330 Fragments arranged by size: 3330 711 59 Acc65I G'GTAC_C Cuts at: 1116 1116 Size: 4100 AclI AA'CG_TT Cuts at: 1094 3940 1094 Size: 2846 1254 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1925 2357 3104 3372 1925 Size: 432 747 268 2653 Fragments arranged by size: 2653 747 432 268 AfeI AGC'GCT Cuts at: 433 961 433 Size: 528 3572 AflIII A'CryG_T Cuts at: 1452 2556 3533 1452 Size: 1104 977 2019 Fragments arranged by size: 2019 1104 977 AgeI A'CCGG_T Cuts at: 2488 2488 Size: 4100 AlwNI CAG_nnn'CTG Cuts at: 1008 1405 2972 1008 Size: 397 1567 2136 Fragments arranged by size: 2136 1567 397 ApaI G_GGCC'C Cuts at: 756 821 756 Size: 65 4035 ApaLI G'TGCA_C Cuts at: 421 2870 421 Size: 2449 1651 ApoI r'AATT_y Cuts at: 1104 3453 3709 1104 Size: 2349 256 1495 Fragments arranged by size: 2349 1495 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4100 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4100 AvaI C'yCGr_G Cuts at: 749 1025 1120 749 Size: 276 95 3729 Fragments arranged by size: 3729 276 95 AvrII C'CTAG_G Cuts at: 906 906 Size: 4100 BamHI G'GATC_C Cuts at: 1125 1125 Size: 4100 BanI G'GyrC_C Cuts at: 132 559 995 1116 1530 1778 1813 132 Size: 427 436 121 414 248 35 2419 Fragments arranged by size: 2419 436 427 414 248 121 35 BanII G_rGCy'C Cuts at: 167 623 737 756 821 1114 2144 167 Size: 456 114 19 65 293 1030 2123 Fragments arranged by size: 2123 1030 456 293 114 65 19 BbeI G_GCGC'C Cuts at: 1534 1782 1534 Size: 248 3852 BciVI GTATCCnnnnn_n' Cuts at: 1998 2759 4100 1998 Size: 761 1341 1998 Fragments arranged by size: 1998 1341 761 BclI T'GATC_A Cuts at: 1620 1620 Size: 4100 BglI GCCn_nnn'nGGC Cuts at: 876 876 Size: 4100 BglII A'GATC_T Cuts at: 1615 3652 1615 Size: 2037 2063 BlpI GC'TnA_GC Cuts at: 1212 1212 Size: 4100 Bme1580I G_kGCm'C Cuts at: 137 425 756 780 821 935 1725 1818 2874 Size: 288 331 24 41 114 790 93 1056 Cuts at: 2874 137 Size: 1363 Fragments arranged by size: 1363 1056 790 331 288 114 93 41 24 BmrI ACTGGGnnnn_n' Cuts at: 883 1312 1393 1728 3872 883 Size: 429 81 335 2144 1111 Fragments arranged by size: 2144 1111 429 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 4100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 442 730 783 806 996 442 Size: 288 53 23 190 3546 Fragments arranged by size: 3546 288 190 53 23 Bpu10I CC'TnA_GC Cuts at: 271 271 Size: 4100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1276 2155 2647 2945 1276 Size: 879 492 298 2431 Fragments arranged by size: 2431 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3804 3804 Size: 4100 BsaAI yAC'GTr Cuts at: 825 1453 825 Size: 628 3472 BsaBI GATnn'nnATC Cuts at: 6 1614 3651 6 Size: 1608 2037 455 Fragments arranged by size: 2037 1608 455 BsaHI Gr'CG_yC Cuts at: 112 1531 1779 2481 3977 112 Size: 1419 248 702 1496 235 Fragments arranged by size: 1496 1419 702 248 235 BsaWI w'CCGG_w Cuts at: 1289 1810 2488 2762 2909 3320 1289 Size: 521 678 274 147 411 2069 Fragments arranged by size: 2069 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 556 586 556 Size: 30 4070 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 352 481 829 889 352 Size: 129 348 60 3563 Fragments arranged by size: 3563 348 129 60 BseYI C'CCAG_C Cuts at: 136 286 588 812 948 1042 1525 2283 2860 Size: 150 302 224 136 94 483 758 577 Cuts at: 2860 3356 136 Size: 496 880 Fragments arranged by size: 880 758 577 496 483 302 224 150 136 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 941 941 Size: 4100 BsiEI CG_ry'CG Cuts at: 104 1642 1688 2896 104 Size: 1538 46 1208 1308 Fragments arranged by size: 1538 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 425 1114 1892 2082 2874 425 Size: 689 778 190 792 1651 Fragments arranged by size: 1651 792 778 689 190 BsmI GAATG_Cn' Cuts at: 473 473 Size: 4100 Bsp1286I G_dGCh'C Cuts at: 137 167 425 623 737 756 780 821 935 Size: 30 258 198 114 19 24 41 114 Cuts at: 935 1114 1725 1818 1892 2082 2144 2874 137 Size: 179 611 93 74 190 62 730 1363 Fragments arranged by size: 1363 730 611 258 198 190 179 114 114 93 74 62 41 30 24 19 BspEI T'CCGG_A Cuts at: 3320 3320 Size: 4100 BspHI T'CATG_A Cuts at: 3276 3276 Size: 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 212 261 1150 1364 1666 2047 212 Size: 49 889 214 302 381 2265 Fragments arranged by size: 2265 889 381 302 214 49 BsrBI CCG'CTC Cuts at: 50 532 714 2392 2446 3930 4095 50 Size: 482 182 1678 54 1484 165 55 Fragments arranged by size: 1678 1484 482 182 165 55 54 BsrDI GCAATG_nn' Cuts at: 307 313 1046 2012 307 Size: 6 733 966 2395 Fragments arranged by size: 2395 966 733 6 BsrFI r'CCGG_y Cuts at: 704 2098 2279 2488 704 Size: 1394 181 209 2316 Fragments arranged by size: 2316 1394 209 181 BssSI C'ACGA_G Cuts at: 2371 2729 2371 Size: 358 3742 BstAPI GCAn_nnn'nTGC Cuts at: 1477 1477 Size: 4100 BstBI TT'CG_AA Cuts at: 2461 2461 Size: 4100 BstXI CCAn_nnnn'nTGG Cuts at: 326 466 948 326 Size: 140 482 3478 Fragments arranged by size: 3478 482 140 BstYI r'GATC_y Cuts at: 1 1125 1592 1615 1950 2196 3208 3294 3306 Size: 1124 467 23 335 246 1012 86 12 Cuts at: 3306 3652 1 Size: 346 449 Fragments arranged by size: 1124 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 448 448 Size: 4100 BtgI C'CryG_G Cuts at: 635 941 635 Size: 306 3794 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 207 743 2050 2231 2394 207 Size: 536 1307 181 163 1913 Fragments arranged by size: 1913 1307 536 181 163 BtsI GCAGTG_nn' Cuts at: 674 725 832 935 1080 1343 674 Size: 51 107 103 145 263 3431 Fragments arranged by size: 3431 263 145 107 103 51 DraI TTT'AAA Cuts at: 1099 1099 Size: 4100 DrdI GACnn_nn'nnGTC Cuts at: 891 1806 2664 891 Size: 915 858 2327 Fragments arranged by size: 2327 915 858 EaeI y'GGCC_r Cuts at: 671 827 1685 1859 2250 2277 3622 671 Size: 156 858 174 391 27 1345 1149 Fragments arranged by size: 1345 1149 858 391 174 156 27 EagI C'GGCC_G Cuts at: 1685 1685 Size: 4100 EarI CTCTTCn'nnn_ Cuts at: 805 1011 2123 2333 805 Size: 206 1112 210 2572 Fragments arranged by size: 2572 1112 210 206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 729 2308 2618 2764 3910 729 Size: 1579 310 146 1146 919 Fragments arranged by size: 1579 1146 919 310 146 EcoICRI GAG'CTC Cuts at: 1112 1112 Size: 4100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 442 730 783 806 996 1925 2357 3104 3372 Size: 288 53 23 190 929 432 747 268 Cuts at: 3372 442 Size: 1170 Fragments arranged by size: 1170 929 747 432 288 268 190 53 23 EcoO109I rG'GnC_Cy Cuts at: 753 817 818 877 1239 753 Size: 64 1 59 362 3614 Fragments arranged by size: 3614 362 64 59 1 EcoRI G'AATT_C Cuts at: 1104 1104 Size: 4100 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2119 2119 Size: 4100 FspI TGC'GCA Cuts at: 244 1881 244 Size: 1637 2463 HaeII r_GCGC'y Cuts at: 435 867 963 1534 1782 2804 3783 435 Size: 432 96 571 248 1022 979 752 Fragments arranged by size: 1022 979 752 571 432 248 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 721 753 1942 1972 1974 2004 721 Size: 32 1189 30 2 30 2817 Fragments arranged by size: 2817 1189 32 30 30 2 HincII GTy'rAC Cuts at: 1080 1139 1080 Size: 59 4041 KasI G'GCGC_C Cuts at: 1530 1778 1530 Size: 248 3852 KpnI G_GTAC'C Cuts at: 1120 1120 Size: 4100 MluI A'CGCG_T Cuts at: 3533 3533 Size: 4100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2771 2955 2771 Size: 184 3916 MscI TGG'CCA Cuts at: 673 829 1861 673 Size: 156 1032 2912 Fragments arranged by size: 2912 1032 156 MslI CAynn'nnrTG Cuts at: 2089 2216 2089 Size: 127 3973 MspA1I CmG'CkG Cuts at: 690 925 1211 1525 1885 2898 3143 690 Size: 235 286 314 360 1013 245 1647 Fragments arranged by size: 1647 1013 360 314 286 245 235 NaeI GCC'GGC Cuts at: 706 2281 706 Size: 1575 2525 NarI GG'CG_CC Cuts at: 1531 1779 1531 Size: 248 3852 NcoI C'CATG_G Cuts at: 941 941 Size: 4100 NgoMIV G'CCGG_C Cuts at: 704 2279 704 Size: 1575 2525 NspI r_CATG'y Cuts at: 1153 1653 2560 1153 Size: 500 907 2693 Fragments arranged by size: 2693 907 500 PasI CC'CwG_GG Cuts at: 216 466 216 Size: 250 3850 PciI A'CATG_T Cuts at: 2556 2556 Size: 4100 PflMI CCAn_nnn'nTGG Cuts at: 256 256 Size: 4100 PmeI GTTT'AAAC Cuts at: 1099 1099 Size: 4100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3239 3271 3239 Size: 32 4068 PspOMI G'GGCC_C Cuts at: 752 817 752 Size: 65 4035 PstI C_TGCA'G Cuts at: 571 695 1004 1147 1832 571 Size: 124 309 143 685 2839 Fragments arranged by size: 2839 685 309 143 124 PvuI CG_AT'CG Cuts at: 104 104 Size: 4100 PvuII CAG'CTG Cuts at: 690 1525 1885 690 Size: 835 360 2905 Fragments arranged by size: 2905 835 360 RsrII CG'GwC_CG Cuts at: 2295 2295 Size: 4100 SacI G_AGCT'C Cuts at: 1114 1114 Size: 4100 SalI G'TCGA_C Cuts at: 1078 1137 1078 Size: 59 4041 SapI GCTCTTCn'nnn_ Cuts at: 805 1011 2123 2333 805 Size: 206 1112 210 2572 Fragments arranged by size: 2572 1112 210 206 SbfI CC_TGCA'GG Cuts at: 1004 1147 1004 Size: 143 3957 ScaI AGT'ACT Cuts at: 3617 3617 Size: 4100 SfcI C'TryA_G Cuts at: 33 567 691 1000 1143 1828 2821 3012 33 Size: 534 124 309 143 685 993 191 1121 Fragments arranged by size: 1121 993 685 534 309 191 143 124 SfoI GGC'GCC Cuts at: 1532 1780 1532 Size: 248 3852 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4100 SmaI CCC'GGG Cuts at: 751 1027 1122 751 Size: 276 95 3729 Fragments arranged by size: 3729 276 95 SmlI C'TyrA_G Cuts at: 1255 2170 2662 2924 1255 Size: 915 492 262 2431 Fragments arranged by size: 2431 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4100 SphI G_CATG'C Cuts at: 1153 1653 1153 Size: 500 3600 SrfI GCCC'GGGC Cuts at: 751 751 Size: 4100 StyI C'CwwG_G Cuts at: 149 906 941 1234 1588 149 Size: 757 35 293 354 2661 Fragments arranged by size: 2661 757 354 293 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 628 1073 1683 1816 2275 2484 628 Size: 445 610 133 459 209 2244 Fragments arranged by size: 2244 610 459 445 209 133 TatI w'GTAC_w Cuts at: 3615 3615 Size: 4100 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 543 575 3617 3649 543 Size: 32 3042 32 994 Fragments arranged by size: 3042 994 32 32 Tth111I GACn'n_nGTC Cuts at: 1897 1897 Size: 4100 XbaI T'CTAG_A Cuts at: 1131 1131 Size: 4100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 156 414 553 1595 156 Size: 258 139 1042 2661 Fragments arranged by size: 2661 1042 258 139 XmaI C'CCGG_G Cuts at: 749 1025 1120 749 Size: 276 95 3729 Fragments arranged by size: 3729 276 95 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AleI AlfI AloI AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI