Restriction map for
pF1KB8995
(Circular) (Six-base) MAPSORT of: pF1KB8995.seq Check: 3637 from: 1 to: 4349
pF1KB8995 4349 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 346 1387 346
Size: 1041 3308
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
AflIII A'CryG_T
Cuts at: 1157 1701 2805 3782 1157
Size: 544 1104 977 1724
Fragments arranged by size:
1724 1104 977 544
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AleI CACnn'nnGTG
Cuts at: 1123 1123
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 175 1141 175
Size: 966 3383
AlwNI CAG_nnn'CTG
Cuts at: 1068 1654 3221 1068
Size: 586 1567 2196
Fragments arranged by size:
2196 1567 586
ApaI G_GGCC'C
Cuts at: 148 1041 1235 148
Size: 893 194 3262
Fragments arranged by size:
3262 893 194
ApaLI G'TGCA_C
Cuts at: 1116 3119 1116
Size: 2003 2346
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 218 1275 1369 218
Size: 1057 94 3198
Fragments arranged by size:
3198 1057 94
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 1365 1779 2027 2062 1365
Size: 414 248 35 3652
Fragments arranged by size:
3652 414 248 35
BanII G_rGCy'C
Cuts at: 148 1041 1235 1276 1295 1363 2393 148
Size: 893 194 41 19 68 1030 2104
Fragments arranged by size:
2104 1030 893 194 68 41 19
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbvCI CC'TCA_GC
Cuts at: 295 295
Size: 4349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4315
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 496 556 586 616 706 766 796 826 856
Size: 60 30 30 90 60 30 30 30
Cuts at: 856 886 916 496
Size: 30 30 3929
Fragments arranged by size:
3929 90 60 60 30 30 30 30
30 30 30
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 148 521 1041 1120 1235 1974 2067 3123 148
Size: 373 520 79 115 739 93 1056 1374
Fragments arranged by size:
1374 1056 739 520 373 115 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 151 335 899 1024 1561 1642 1977 4121 151
Size: 184 564 125 537 81 335 2144 379
Fragments arranged by size:
2144 564 537 379 335 184 125 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1251 2716 1251
Size: 1465 2884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 694 269
Size: 425 3924
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1285 1780 2028 2730 4226 1285
Size: 495 248 702 1496 1408
Fragments arranged by size:
1496 1408 702 495 248
BsaWI w'CCGG_w
Cuts at: 224 1538 2059 2737 3011 3158 3569 224
Size: 1314 521 678 274 147 411 1004
Fragments arranged by size:
1314 1004 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 267 297 386 416 877 907 267
Size: 30 89 30 461 30 3709
Fragments arranged by size:
3709 461 89 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 112 308 112
Size: 196 4153
BseYI C'CCAG_C
Cuts at: 1774 2532 3109 3605 1774
Size: 758 577 496 2518
Fragments arranged by size:
2518 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 704 794 1312 674
Size: 30 90 518 3711
Fragments arranged by size:
3711 518 90 30
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1363 2141 2331 3123 1120
Size: 243 778 190 792 2346
Fragments arranged by size:
2346 792 778 243 190
BsmI GAATG_Cn'
Cuts at: 992 992
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 148 521 1041 1120 1235 1276 1295 1363 1974
Size: 373 520 79 115 41 19 68 611
Cuts at: 1974 2067 2141 2331 2393 3123 148
Size: 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 520 373 190 115 93
79 74 68 62 41 19
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 1613 1915 2296 1399
Size: 214 302 381 3452
Fragments arranged by size:
3452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 2347 2528 2737 2347
Size: 181 209 3959
Fragments arranged by size:
3959 209 181
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1068 1726 1068
Size: 658 3691
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 423 543 603 672 792 873 1191 423
Size: 120 60 69 120 81 318 3581
Fragments arranged by size:
3581 318 120 120 81 69 60
BstYI r'GATC_y
Cuts at: 1 1374 1841 1864 2199 2445 3457 3543 3555
Size: 1373 467 23 335 246 1012 86 12
Cuts at: 3555 3901 1
Size: 346 449
Fragments arranged by size:
1373 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 416 446 491 521 581 641 701 731 761
Size: 30 45 30 60 60 60 30 30
Cuts at: 761 791 821 851 881 926 998 416
Size: 30 30 30 30 45 72 3767
Fragments arranged by size:
3767 72 60 60 60 45 45 30
30 30 30 30 30 30 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2299 2480 2643 2299
Size: 181 163 4005
Fragments arranged by size:
4005 181 163
BtsI GCAGTG_nn'
Cuts at: 686 716 806 1069 1592 686
Size: 30 90 263 523 3443
Fragments arranged by size:
3443 523 263 90 30
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 425 578 1073 1097 1934 2108 2499 2526 3871
Size: 153 495 24 837 174 391 27 1345
Cuts at: 3871 425
Size: 903
Fragments arranged by size:
1345 903 837 495 391 174 153 27
24
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1126 2557 2867 3013 4159 1126
Size: 1431 310 146 1146 1316
Fragments arranged by size:
1431 1316 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 694 2174 2606 3353 3621 269
Size: 425 1480 432 747 268 997
Fragments arranged by size:
1480 997 747 432 425 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1261 1261
Size: 4349
EcoO109I rG'GnC_Cy
Cuts at: 145 205 888 918 1037 1038 1231 1232 1254
Size: 60 683 30 119 1 193 1 22
Cuts at: 1254 1488 145
Size: 234 3006
Fragments arranged by size:
3006 683 234 193 119 60 30 22
1 1
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 1093 1783 2031 3053 4032 1093
Size: 690 248 1022 979 1410
Fragments arranged by size:
1410 1022 979 690 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 386 418 877 907 909 939 1036 1068 2191
Size: 32 459 30 2 30 97 32 1123
Cuts at: 2191 2221 2223 2253 386
Size: 30 2 30 2482
Fragments arranged by size:
2482 1123 459 97 32 32 30 30
30 30 2 2
HincII GTy'rAC
Cuts at: 1023 1388 1023
Size: 365 3984
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3204 3020
Size: 184 4165
MscI TGG'CCA
Cuts at: 427 1075 1099 2110 427
Size: 648 24 1011 2666
Fragments arranged by size:
2666 1011 648 24
MslI CAynn'nnrTG
Cuts at: 790 997 1054 1123 1189 2338 2465 790
Size: 207 57 69 66 1149 127 2674
Fragments arranged by size:
2674 1149 207 127 69 66 57
MspA1I CmG'CkG
Cuts at: 165 1460 1774 2134 3147 3392 165
Size: 1295 314 360 1013 245 1122
Fragments arranged by size:
1295 1122 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 701 731 761 791 881 926 998 701
Size: 30 30 30 90 45 72 4052
Fragments arranged by size:
4052 90 72 45 30 30 30
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 1161 1402 1902 2809 1161
Size: 241 500 907 2701
Fragments arranged by size:
2701 907 500 241
PasI CC'CwG_GG
Cuts at: 1295 1295
Size: 4349
PciI A'CATG_T
Cuts at: 1157 2805 1157
Size: 1648 2701
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 205 205
Size: 4349
PspOMI G'GGCC_C
Cuts at: 144 1037 1231 144
Size: 893 194 3262
Fragments arranged by size:
3262 893 194
PstI C_TGCA'G
Cuts at: 1396 2081 1396
Size: 685 3664
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 165 1774 2134 165
Size: 1609 360 2380
Fragments arranged by size:
2380 1609 360
RsrII CG'GwC_CG
Cuts at: 1134 2544 1134
Size: 1410 2939
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 205 205
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 1392 2077 3070 3261 33
Size: 1359 685 993 191 1121
Fragments arranged by size:
1359 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 220 1277 1371 220
Size: 1057 94 3198
Fragments arranged by size:
3198 1057 94
SmlI C'TyrA_G
Cuts at: 1504 2419 2911 3173 1504
Size: 915 492 262 2680
Fragments arranged by size:
2680 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
SrfI GCCC'GGGC
Cuts at: 1277 1277
Size: 4349
StuI AGG'CCT
Cuts at: 671 671
Size: 4349
StyI C'CwwG_G
Cuts at: 200 701 731 761 791 881 926 998 1262
Size: 501 30 30 30 90 45 72 264
Cuts at: 1262 1483 1837 200
Size: 221 354 2712
Fragments arranged by size:
2712 501 354 264 221 90 72 45
30 30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1932 2065 2524 2733 1932
Size: 133 459 209 3548
Fragments arranged by size:
3548 459 209 133
TatI w'GTAC_w
Cuts at: 3864 3864
Size: 4349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1083 1844 1083
Size: 761 3588
XmaI C'CCGG_G
Cuts at: 218 1275 1369 218
Size: 1057 94 3198
Fragments arranged by size:
3198 1057 94
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI