Restriction map for
pF1KB8998
(Circular) (Six-base) MAPSORT of: pF1KB8998.seq Check: 3269 from: 1 to: 4349
pF1KB8998 4349 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 751 2174 2606 3353 3621 369
Size: 382 1423 432 747 268 1097
Fragments arranged by size:
1423 1097 747 432 382 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 753 1654 3221 753
Size: 901 1567 1881
Fragments arranged by size:
1881 1567 901
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 1070 1369 1070
Size: 299 4050
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 669 1060 1365 1779 2027 2062 669
Size: 391 305 414 248 35 2956
Fragments arranged by size:
2956 414 391 305 248 35
BanII G_rGCy'C
Cuts at: 124 379 830 1262 1277 1298 1363 2393 124
Size: 255 451 432 15 21 65 1030 2080
Fragments arranged by size:
2080 1030 451 432 255 65 21 15
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4315
BciVI GTATCCnnnnn_n'
Cuts at: 991 2247 3008 4349 991
Size: 1256 761 1341 991
Fragments arranged by size:
1341 1256 991 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 674 771 1071 1221 1291 1974 2067 3123 674
Size: 97 300 150 70 683 93 1056 1900
Fragments arranged by size:
1900 1056 683 300 150 97 93 70
BmgBI CAC'GTC
Cuts at: 1026 1254 1026
Size: 228 4121
BmrI ACTGGGnnnn_n'
Cuts at: 1561 1642 1977 4121 1561
Size: 81 335 2144 1789
Fragments arranged by size:
2144 1789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 767 2716 767
Size: 1949 2400
Bpu10I CC'TnA_GC
Cuts at: 470 1169 1264 470
Size: 699 95 3555
Fragments arranged by size:
3555 699 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1525 2404 2896 3194 1261
Size: 264 879 492 298 2416
Fragments arranged by size:
2416 879 492 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 437 1538 2059 2737 3011 3158 3569 437
Size: 1101 521 678 274 147 411 1217
Fragments arranged by size:
1217 1101 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1091 1121 1322 1328 1352 1358 1091
Size: 30 201 6 24 6 4082
Fragments arranged by size:
4082 201 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 114 114
Size: 4349
BseYI C'CCAG_C
Cuts at: 191 491 687 1774 2532 3109 3605 191
Size: 300 196 1087 758 577 496 935
Fragments arranged by size:
1087 935 758 577 496 300 196
BsiEI CG_ry'CG
Cuts at: 104 260 1891 1937 3145 104
Size: 156 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 156 46
BsiHKAI G_wGCw'C
Cuts at: 281 379 1161 1262 1277 1363 2141 2331 3123
Size: 98 782 101 15 86 778 190 792
Cuts at: 3123 281
Size: 1507
Fragments arranged by size:
1507 792 782 778 190 101 98 86
15
BsmBI CGTCTCn'nnnn_
Cuts at: 1123 1123
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 124 281 379 674 771 830 1071 1161 1221
Size: 157 98 295 97 59 241 90 60
Cuts at: 1221 1262 1277 1291 1298 1363 1974 2067 2141
Size: 41 15 14 7 65 611 93 74
Cuts at: 2141 2331 2393 3123 124
Size: 190 62 730 1350
Fragments arranged by size:
1350 730 611 295 241 190 157 98
97 93 90 74 65 62 60 59
41 15 14 7
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 1095 1399 1613 1915 2296 224
Size: 871 304 214 302 381 2277
Fragments arranged by size:
2277 871 381 304 302 214
BsrBI CCG'CTC
Cuts at: 50 435 765 1173 2641 2695 4179 4344 50
Size: 385 330 408 1468 54 1484 165 55
Fragments arranged by size:
1484 1468 408 385 330 165 55 54
BsrDI GCAATG_nn'
Cuts at: 152 183 344 416 479 2261 152
Size: 31 161 72 63 1782 2240
Fragments arranged by size:
2240 1782 161 72 63 31
BsrFI r'CCGG_y
Cuts at: 457 2347 2528 2737 457
Size: 1890 181 209 2069
Fragments arranged by size:
2069 1890 209 181
BsrGI T'GTAC_A
Cuts at: 131 131
Size: 4349
BssSI C'ACGA_G
Cuts at: 462 2620 2978 462
Size: 2158 358 1833
Fragments arranged by size:
2158 1833 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstEII G'GTnAC_C
Cuts at: 453 453
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 810 822 1142 810
Size: 12 320 4017
Fragments arranged by size:
4017 320 12
BstYI r'GATC_y
Cuts at: 1 704 1374 1841 1864 2199 2445 3457 3543
Size: 703 670 467 23 335 246 1012 86
Cuts at: 3543 3555 3901 1
Size: 12 346 449
Fragments arranged by size:
1012 703 670 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1030 1030
Size: 4349
BtgI C'CryG_G
Cuts at: 330 330
Size: 4349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 249 384 2299 2480 2643 249
Size: 135 1915 181 163 1955
Fragments arranged by size:
1955 1915 181 163 135
BtsI GCAGTG_nn'
Cuts at: 275 778 1098 1161 1592 275
Size: 503 320 63 431 3032
Fragments arranged by size:
3032 503 431 320 63
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 149 164 257 395 812 923 1125 1934 2108
Size: 15 93 138 417 111 202 809 174
Cuts at: 2108 2499 2526 3871 149
Size: 391 27 1345 627
Fragments arranged by size:
1345 809 627 417 391 202 174 138
111 93 27 15
EagI C'GGCC_G
Cuts at: 257 1934 257
Size: 1677 2672
EarI CTCTTCn'nnn_
Cuts at: 346 2372 2582 346
Size: 2026 210 2113
Fragments arranged by size:
2113 2026 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 982 2557 2867 3013 4159 982
Size: 1575 310 146 1146 1172
Fragments arranged by size:
1575 1172 1146 310 146
EcoICRI GAG'CTC
Cuts at: 377 1260 1275 1361 377
Size: 883 15 86 3365
Fragments arranged by size:
3365 883 86 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 751 2174 2606 3353 3621 369
Size: 382 1423 432 747 268 1097
Fragments arranged by size:
1423 1097 747 432 382 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1321 1321
Size: 4349
EcoO109I rG'GnC_Cy
Cuts at: 1488 1488
Size: 4349
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 1783 2031 3053 4032 1783
Size: 248 1022 979 2100
Fragments arranged by size:
2100 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1091 1123 2191 2221 2223 2253 1091
Size: 32 1068 30 2 30 3187
Fragments arranged by size:
3187 1068 32 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 514 3020 3204 514
Size: 2506 184 1659
Fragments arranged by size:
2506 1659 184
MscI TGG'CCA
Cuts at: 151 166 397 814 925 2110 151
Size: 15 231 417 111 1185 2390
Fragments arranged by size:
2390 1185 417 231 111 15
MslI CAynn'nnrTG
Cuts at: 139 157 268 820 1091 2338 2465 139
Size: 18 111 552 271 1247 127 2023
Fragments arranged by size:
2023 1247 552 271 127 111 18
MspA1I CmG'CkG
Cuts at: 729 1057 1460 1774 2134 3147 3392 729
Size: 328 403 314 360 1013 245 1686
Fragments arranged by size:
1686 1013 403 360 328 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 1402 1902 2809 1402
Size: 500 907 2942
Fragments arranged by size:
2942 907 500
PasI CC'CwG_GG
Cuts at: 576 1194 1230 576
Size: 618 36 3695
Fragments arranged by size:
3695 618 36
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 491 491
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PstI C_TGCA'G
Cuts at: 221 785 895 1396 2081 221
Size: 564 110 501 685 2489
Fragments arranged by size:
2489 685 564 501 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 729 1057 1774 2134 729
Size: 328 717 360 2944
Fragments arranged by size:
2944 717 360 328
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 379 1262 1277 1363 379
Size: 883 15 86 3365
Fragments arranged by size:
3365 883 86 15
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 217 781 891 1392 2077 3070 3261 33
Size: 184 564 110 501 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 501 191 184 110
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1072 1371 1072
Size: 299 4050
SmlI C'TyrA_G
Cuts at: 1276 1504 2419 2911 3173 1276
Size: 228 915 492 262 2452
Fragments arranged by size:
2452 915 492 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 1483 1837 1483
Size: 354 3995
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 483 679 1932 2065 2524 2733 262
Size: 221 196 1253 133 459 209 1878
Fragments arranged by size:
1878 1253 459 221 209 196 133
TatI w'GTAC_w
Cuts at: 131 641 3864 131
Size: 510 3223 616
Fragments arranged by size:
3223 616 510
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1322 1354 3866 3898 1322
Size: 32 2512 32 1773
Fragments arranged by size:
2512 1773 32 32
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 159 237 633 1844 114
Size: 45 78 396 1211 2619
Fragments arranged by size:
2619 1211 396 78 45
XmaI C'CCGG_G
Cuts at: 1070 1369 1070
Size: 299 4050
XmnI GAAnn'nnTTC
Cuts at: 961 961
Size: 4349
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmtI BpmI BsgI BsiWI BsmI BssHII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI