(Circular) (Six-base) MAPSORT of: pF1KB8999.seq Check: 4508 from: 1 to: 4184 pF1KB8999 4184 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 1222 1222 Size: 4184 Acc65I G'GTAC_C Cuts at: 1200 1200 Size: 4184 AclI AA'CG_TT Cuts at: 4024 4024 Size: 4184 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2009 2441 3188 3456 2009 Size: 432 747 268 2737 Fragments arranged by size: 2737 747 432 268 AflIII A'CryG_T Cuts at: 926 1176 1536 2640 3617 926 Size: 250 360 1104 977 1493 Fragments arranged by size: 1493 1104 977 360 250 AgeI A'CCGG_T Cuts at: 2572 2572 Size: 4184 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 115 147 115 Size: 32 4152 AlwNI CAG_nnn'CTG Cuts at: 494 1489 3056 494 Size: 995 1567 1622 Fragments arranged by size: 1622 1567 995 ApaLI G'TGCA_C Cuts at: 403 2954 403 Size: 2551 1633 ApoI r'AATT_y Cuts at: 1188 3537 3793 1188 Size: 2349 256 1579 Fragments arranged by size: 2349 1579 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4184 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4184 AvaI C'yCGr_G Cuts at: 1204 1204 Size: 4184 BamHI G'GATC_C Cuts at: 1001 1209 1001 Size: 208 3976 BanI G'GyrC_C Cuts at: 419 727 1200 1614 1862 1897 419 Size: 308 473 414 248 35 2706 Fragments arranged by size: 2706 473 414 308 248 35 BanII G_rGCy'C Cuts at: 805 1025 1198 2228 805 Size: 220 173 1030 2761 Fragments arranged by size: 2761 1030 220 173 BbeI G_GCGC'C Cuts at: 423 731 1618 1866 423 Size: 308 887 248 2741 Fragments arranged by size: 2741 887 308 248 BbsI GAAGACnn'nnnn_ Cuts at: 213 817 213 Size: 604 3580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 418 452 418 Size: 34 4150 BciVI GTATCCnnnnn_n' Cuts at: 2082 2843 4184 2082 Size: 761 1341 2082 Fragments arranged by size: 2082 1341 761 BclI T'GATC_A Cuts at: 1704 1704 Size: 4184 BglII A'GATC_T Cuts at: 773 1699 3736 773 Size: 926 2037 1221 Fragments arranged by size: 2037 1221 926 BlpI GC'TnA_GC Cuts at: 394 1296 394 Size: 902 3282 Bme1580I G_kGCm'C Cuts at: 332 407 609 1809 1902 2958 332 Size: 75 202 1200 93 1056 1558 Fragments arranged by size: 1558 1200 1056 202 93 75 BmgBI CAC'GTC Cuts at: 471 471 Size: 4184 BmrI ACTGGGnnnn_n' Cuts at: 1396 1477 1812 3956 1396 Size: 81 335 2144 1624 Fragments arranged by size: 2144 1624 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 678 2551 678 Size: 1873 2311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 789 789 Size: 4184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1360 2239 2731 3029 1360 Size: 879 492 298 2515 Fragments arranged by size: 2515 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 157 3888 157 Size: 3731 453 BsaAI yAC'GTr Cuts at: 929 1537 929 Size: 608 3576 BsaBI GATnn'nnATC Cuts at: 6 1698 3735 6 Size: 1692 2037 455 Fragments arranged by size: 2037 1692 455 BsaHI Gr'CG_yC Cuts at: 420 728 1615 1863 2565 4061 420 Size: 308 887 248 702 1496 543 Fragments arranged by size: 1496 887 702 543 308 248 BsaWI w'CCGG_w Cuts at: 1373 1894 2572 2846 2993 3404 1373 Size: 521 678 274 147 411 2153 Fragments arranged by size: 2153 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 115 145 1081 1111 115 Size: 30 936 30 3188 Fragments arranged by size: 3188 936 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 691 691 Size: 4184 BseYI C'CCAG_C Cuts at: 199 246 647 1609 2367 2944 3440 199 Size: 47 401 962 758 577 496 943 Fragments arranged by size: 962 943 758 577 496 401 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 465 465 Size: 4184 BsiEI CG_ry'CG Cuts at: 104 1726 1772 2980 104 Size: 1622 46 1208 1308 Fragments arranged by size: 1622 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 407 758 995 1025 1198 1976 2166 2958 407 Size: 351 237 30 173 778 190 792 1633 Fragments arranged by size: 1633 792 778 351 237 190 173 30 BsmBI CGTCTCn'nnnn_ Cuts at: 363 582 649 828 363 Size: 219 67 179 3719 Fragments arranged by size: 3719 219 179 67 Bsp1286I G_dGCh'C Cuts at: 332 407 609 758 805 995 1025 1198 1809 Size: 75 202 149 47 190 30 173 611 Cuts at: 1809 1902 1976 2166 2228 2958 332 Size: 93 74 190 62 730 1558 Fragments arranged by size: 1558 730 611 202 190 190 173 149 93 75 74 62 47 30 BspEI T'CCGG_A Cuts at: 3404 3404 Size: 4184 BspHI T'CATG_A Cuts at: 587 3360 587 Size: 2773 1411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 200 290 746 1234 1448 1750 2131 200 Size: 90 456 488 214 302 381 2253 Fragments arranged by size: 2253 488 456 381 302 214 90 BsrBI CCG'CTC Cuts at: 50 744 2476 2530 4014 4179 50 Size: 694 1732 54 1484 165 55 Fragments arranged by size: 1732 1484 694 165 55 54 BsrDI GCAATG_nn' Cuts at: 2096 2096 Size: 4184 BsrFI r'CCGG_y Cuts at: 2182 2363 2572 2182 Size: 181 209 3794 Fragments arranged by size: 3794 209 181 BssSI C'ACGA_G Cuts at: 406 2455 2813 406 Size: 2049 358 1777 Fragments arranged by size: 2049 1777 358 BstAPI GCAn_nnn'nTGC Cuts at: 1561 1561 Size: 4184 BstBI TT'CG_AA Cuts at: 2545 2545 Size: 4184 BstXI CCAn_nnnn'nTGG Cuts at: 516 516 Size: 4184 BstYI r'GATC_y Cuts at: 1 773 1001 1209 1676 1699 2034 2280 3292 Size: 772 228 208 467 23 335 246 1012 Cuts at: 3292 3378 3390 3736 1 Size: 86 12 346 449 Fragments arranged by size: 1012 772 467 449 346 335 246 228 208 86 23 12 BtgI C'CryG_G Cuts at: 107 350 107 Size: 243 3941 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2134 2315 2478 2134 Size: 181 163 3840 Fragments arranged by size: 3840 181 163 BtsI GCAGTG_nn' Cuts at: 1427 1427 Size: 4184 DraI TTT'AAA Cuts at: 1183 1183 Size: 4184 DraIII CAC_nnn'GTG Cuts at: 327 327 Size: 4184 DrdI GACnn_nn'nnGTC Cuts at: 1890 2748 1890 Size: 858 3326 EaeI y'GGCC_r Cuts at: 353 374 485 506 1769 1943 2334 2361 3706 Size: 21 111 21 1263 174 391 27 1345 Cuts at: 3706 353 Size: 831 Fragments arranged by size: 1345 1263 831 391 174 111 27 21 21 EagI C'GGCC_G Cuts at: 1769 1769 Size: 4184 EarI CTCTTCn'nnn_ Cuts at: 763 2207 2417 763 Size: 1444 210 2530 Fragments arranged by size: 2530 1444 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 880 2392 2702 2848 3994 880 Size: 1512 310 146 1146 1070 Fragments arranged by size: 1512 1146 1070 310 146 EcoICRI GAG'CTC Cuts at: 1023 1196 1023 Size: 173 4011 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 789 2009 2441 3188 3456 789 Size: 1220 432 747 268 1517 Fragments arranged by size: 1517 1220 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1323 1323 Size: 4184 EcoRI G'AATT_C Cuts at: 1188 1188 Size: 4184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1179 2203 1179 Size: 1024 3160 FspI TGC'GCA Cuts at: 1965 1965 Size: 4184 HaeII r_GCGC'y Cuts at: 423 731 1151 1618 1866 2888 3867 423 Size: 308 420 467 248 1022 979 740 Fragments arranged by size: 1022 979 740 467 420 308 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2026 2056 2058 2088 2026 Size: 30 2 30 4122 Fragments arranged by size: 4122 30 30 2 HincII GTy'rAC Cuts at: 1223 1223 Size: 4184 KasI G'GCGC_C Cuts at: 419 727 1614 1862 419 Size: 308 887 248 2741 Fragments arranged by size: 2741 887 308 248 KpnI G_GTAC'C Cuts at: 1204 1204 Size: 4184 MluI A'CGCG_T Cuts at: 3617 3617 Size: 4184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 418 605 1118 2855 3039 418 Size: 187 513 1737 184 1563 Fragments arranged by size: 1737 1563 513 187 184 MscI TGG'CCA Cuts at: 1945 1945 Size: 4184 MslI CAynn'nnrTG Cuts at: 982 2173 2300 982 Size: 1191 127 2866 Fragments arranged by size: 2866 1191 127 MspA1I CmG'CkG Cuts at: 393 425 616 636 951 1295 1609 1969 2982 Size: 32 191 20 315 344 314 360 1013 Cuts at: 2982 3227 393 Size: 245 1350 Fragments arranged by size: 1350 1013 360 344 315 314 245 191 32 20 NaeI GCC'GGC Cuts at: 2365 2365 Size: 4184 NarI GG'CG_CC Cuts at: 420 728 1615 1863 420 Size: 308 887 248 2741 Fragments arranged by size: 2741 887 308 248 NcoI C'CATG_G Cuts at: 107 350 107 Size: 243 3941 NgoMIV G'CCGG_C Cuts at: 2363 2363 Size: 4184 NspI r_CATG'y Cuts at: 1237 1737 2644 1237 Size: 500 907 2777 Fragments arranged by size: 2777 907 500 PciI A'CATG_T Cuts at: 2640 2640 Size: 4184 PflMI CCAn_nnn'nTGG Cuts at: 339 494 339 Size: 155 4029 PfoI T'CCnGG_A Cuts at: 805 805 Size: 4184 PmeI GTTT'AAAC Cuts at: 1183 1183 Size: 4184 PmlI CAC'GTG Cuts at: 929 929 Size: 4184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 115 147 3323 3355 115 Size: 32 3176 32 944 Fragments arranged by size: 3176 944 32 32 PstI C_TGCA'G Cuts at: 1231 1916 1231 Size: 685 3499 PvuI CG_AT'CG Cuts at: 104 104 Size: 4184 PvuII CAG'CTG Cuts at: 1609 1969 1609 Size: 360 3824 RsrII CG'GwC_CG Cuts at: 888 2379 888 Size: 1491 2693 SacI G_AGCT'C Cuts at: 1025 1198 1025 Size: 173 4011 SalI G'TCGA_C Cuts at: 1221 1221 Size: 4184 SapI GCTCTTCn'nnn_ Cuts at: 763 2207 2417 763 Size: 1444 210 2530 Fragments arranged by size: 2530 1444 210 SbfI CC_TGCA'GG Cuts at: 1231 1231 Size: 4184 ScaI AGT'ACT Cuts at: 3701 3701 Size: 4184 SexAI A'CCwGG_T Cuts at: 338 338 Size: 4184 SfcI C'TryA_G Cuts at: 33 1227 1912 2905 3096 33 Size: 1194 685 993 191 1121 Fragments arranged by size: 1194 1121 993 685 191 SfoI GGC'GCC Cuts at: 421 729 1616 1864 421 Size: 308 887 248 2741 Fragments arranged by size: 2741 887 308 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4184 SmaI CCC'GGG Cuts at: 1206 1206 Size: 4184 SmlI C'TyrA_G Cuts at: 1339 2254 2746 3008 1339 Size: 915 492 262 2515 Fragments arranged by size: 2515 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4184 SphI G_CATG'C Cuts at: 1237 1737 1237 Size: 500 3684 StyI C'CwwG_G Cuts at: 107 350 1318 1672 107 Size: 243 968 354 2619 Fragments arranged by size: 2619 968 354 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 207 843 1767 1900 2359 2568 207 Size: 636 924 133 459 209 1823 Fragments arranged by size: 1823 924 636 459 209 133 TatI w'GTAC_w Cuts at: 764 3699 764 Size: 2935 1249 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 397 429 3701 3733 397 Size: 32 3272 32 848 Fragments arranged by size: 3272 848 32 32 Tth111I GACn'n_nGTC Cuts at: 1981 1981 Size: 4184 XbaI T'CTAG_A Cuts at: 1215 1215 Size: 4184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 795 1679 795 Size: 884 3300 XmaI C'CCGG_G Cuts at: 1204 1204 Size: 4184 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI