Restriction map for
pF1KB9000
(Circular) (Six-base) MAPSORT of: pF1KB9000.seq Check: 9490 from: 1 to: 4367
pF1KB9000 4367 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 489 503 489
Size: 14 4353
AatII G_ACGT'C
Cuts at: 358 358
Size: 4367
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 805 1383 805
Size: 578 3789
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2624 3371 3639 2192
Size: 432 747 268 2920
Fragments arranged by size:
2920 747 432 268
AflIII A'CryG_T
Cuts at: 1151 1719 2823 3800 1151
Size: 568 1104 977 1718
Fragments arranged by size:
1718 1104 977 568
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 340 340
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 423 1672 3239 423
Size: 1249 1567 1551
Fragments arranged by size:
1567 1551 1249
ApaI G_GGCC'C
Cuts at: 140 140
Size: 4367
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 1222 1342 1371 3720 3976 1222
Size: 120 29 2349 256 1613
Fragments arranged by size:
2349 1613 256 120 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 141 211 956 1387 141
Size: 70 745 431 3121
Fragments arranged by size:
3121 745 431 70
BamHI G'GATC_C
Cuts at: 1056 1392 1056
Size: 336 4031
BanI G'GyrC_C
Cuts at: 786 805 1020 1383 1797 2045 2080 786
Size: 19 215 363 414 248 35 3073
Fragments arranged by size:
3073 414 363 248 215 35 19
BanII G_rGCy'C
Cuts at: 140 1381 2411 140
Size: 1241 1030 2096
Fragments arranged by size:
2096 1241 1030
BbeI G_GCGC'C
Cuts at: 790 1024 1801 2049 790
Size: 234 777 248 3108
Fragments arranged by size:
3108 777 248 234
BbsI GAAGACnn'nnnn_
Cuts at: 663 862 1263 663
Size: 199 401 3767
Fragments arranged by size:
3767 401 199
BbvCI CC'TCA_GC
Cuts at: 385 385
Size: 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 222 256 90
Size: 34 98 34 4201
Fragments arranged by size:
4201 98 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 280 400 280
Size: 120 4247
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 637 649 1479 637
Size: 12 830 3525
Fragments arranged by size:
3525 830 12
Bme1580I G_kGCm'C
Cuts at: 140 1992 2085 3141 140
Size: 1852 93 1056 1366
Fragments arranged by size:
1852 1366 1056 93
BmgBI CAC'GTC
Cuts at: 240 240
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1579 1660 1995 4139 1123
Size: 456 81 335 2144 1351
Fragments arranged by size:
2144 1351 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 286 135
Size: 151 4216
Bpu10I CC'TnA_GC
Cuts at: 385 385
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1543 2422 2914 3212 562
Size: 981 879 492 298 1717
Fragments arranged by size:
1717 981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 756 1720 756
Size: 964 3403
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 343 355 787 862 1021 1798 2046 2748 4244
Size: 12 432 75 159 777 248 702 1496
Cuts at: 4244 343
Size: 466
Fragments arranged by size:
1496 777 702 466 432 248 159 75
12
BsaWI w'CCGG_w
Cuts at: 338 1556 2077 2755 3029 3176 3587 338
Size: 1218 521 678 274 147 411 1118
Fragments arranged by size:
1218 1118 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 265
Size: 4367
BseYI C'CCAG_C
Cuts at: 306 437 716 1016 1792 2550 3127 3623 306
Size: 131 279 300 776 758 577 496 1050
Fragments arranged by size:
1050 776 758 577 496 300 279 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 902 319
Size: 583 3784
BsiEI CG_ry'CG
Cuts at: 104 552 626 1909 1955 3163 104
Size: 448 74 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 448 74 46
BsiHKAI G_wGCw'C
Cuts at: 655 1381 2159 2349 3141 655
Size: 726 778 190 792 1881
Fragments arranged by size:
1881 792 778 726 190
BsmI GAATG_Cn'
Cuts at: 409 409
Size: 4367
BsmBI CGTCTCn'nnnn_
Cuts at: 118 118
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 140 655 1381 1992 2085 2159 2349 2411 3141
Size: 515 726 611 93 74 190 62 730
Cuts at: 3141 140
Size: 1366
Fragments arranged by size:
1366 730 726 611 515 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 539 614 3543 539
Size: 75 2929 1363
Fragments arranged by size:
2929 1363 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 503 817 1417 1631 1933 2314 489
Size: 14 314 600 214 302 381 2542
Fragments arranged by size:
2542 600 381 314 302 214 14
BsrBI CCG'CTC
Cuts at: 50 576 605 2659 2713 4197 4362 50
Size: 526 29 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 526 165 55 54 29
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 121 175 2365 2546 2755 121
Size: 54 2190 181 209 1733
Fragments arranged by size:
2190 1733 209 181 54
BssHII G'CGCG_C
Cuts at: 598 598
Size: 4367
BssSI C'ACGA_G
Cuts at: 746 2638 2996 746
Size: 1892 358 2117
Fragments arranged by size:
2117 1892 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 965 1056 1392 1859 1882 2217 2463 3475
Size: 964 91 336 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 964 467 449 346 336 335 246
91 86 23 12
BtgI C'CryG_G
Cuts at: 157 404 459 791 849 157
Size: 247 55 332 58 3675
Fragments arranged by size:
3675 332 247 58 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 543 2317 2498 2661 543
Size: 1774 181 163 2249
Fragments arranged by size:
2249 1774 181 163
BtsI GCAGTG_nn'
Cuts at: 1103 1610 1103
Size: 507 3860
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DraIII CAC_nnn'GTG
Cuts at: 931 931
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 170 257 299 392 401 623 1164 1952 2126
Size: 87 42 93 9 222 541 788 174
Cuts at: 2126 2517 2544 3889 170
Size: 391 27 1345 648
Fragments arranged by size:
1345 788 648 541 391 222 174 93
87 42 27 9
EagI C'GGCC_G
Cuts at: 623 1952 623
Size: 1329 3038
EarI CTCTTCn'nnn_
Cuts at: 376 2390 2600 376
Size: 2014 210 2143
Fragments arranged by size:
2143 2014 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 108 2575 2885 3031 4177 108
Size: 2467 310 146 1146 298
Fragments arranged by size:
2467 1146 310 298 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 286 2192 2624 3371 3639 135
Size: 151 1906 432 747 268 863
Fragments arranged by size:
1906 863 747 432 268 151
EcoNI CCTnn'n_nnAGG
Cuts at: 214 214
Size: 4367
EcoO109I rG'GnC_Cy
Cuts at: 136 137 1506 136
Size: 1 1369 2997
Fragments arranged by size:
2997 1369 1
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1280 2386 1280
Size: 1106 3261
FspI TGC'GCA
Cuts at: 244 2148 244
Size: 1904 2463
HaeII r_GCGC'y
Cuts at: 182 790 1024 1801 2049 3071 4050 182
Size: 608 234 777 248 1022 979 499
Fragments arranged by size:
1022 979 777 608 499 248 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1048 1080 2209 2239 2241 2271 1048
Size: 32 1129 30 2 30 3144
Fragments arranged by size:
3144 1129 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
HindIII A'AGCT_T
Cuts at: 1191 1191
Size: 4367
KasI G'GCGC_C
Cuts at: 786 1020 1797 2045 786
Size: 234 777 248 3108
Fragments arranged by size:
3108 777 248 234
KpnI G_GTAC'C
Cuts at: 809 1387 809
Size: 578 3789
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 402 3038 3222 402
Size: 2636 184 1547
Fragments arranged by size:
2636 1547 184
MscI TGG'CCA
Cuts at: 172 259 1166 2128 172
Size: 87 907 962 2411
Fragments arranged by size:
2411 962 907 87
MslI CAynn'nnrTG
Cuts at: 562 781 1039 2356 2483 562
Size: 219 258 1317 127 2446
Fragments arranged by size:
2446 1317 258 219 127
MspA1I CmG'CkG
Cuts at: 461 851 1478 1792 2152 3165 3410 461
Size: 390 627 314 360 1013 245 1418
Fragments arranged by size:
1418 1013 627 390 360 314 245
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 787 1021 1798 2046 787
Size: 234 777 248 3108
Fragments arranged by size:
3108 777 248 234
NcoI C'CATG_G
Cuts at: 157 157
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NotI GC'GGCC_GC
Cuts at: 623 623
Size: 4367
NspI r_CATG'y
Cuts at: 366 513 531 1155 1420 1920 2827 366
Size: 147 18 624 265 500 907 1906
Fragments arranged by size:
1906 907 624 500 265 147 18
PasI CC'CwG_GG
Cuts at: 737 737
Size: 4367
PciI A'CATG_T
Cuts at: 1151 2823 1151
Size: 1672 2695
PflMI CCAn_nnn'nTGG
Cuts at: 158 158
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PspOMI G'GGCC_C
Cuts at: 136 136
Size: 4367
PstI C_TGCA'G
Cuts at: 500 1414 2099 500
Size: 914 685 2768
Fragments arranged by size:
2768 914 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SacII CC_GC'GG
Cuts at: 462 852 462
Size: 390 3977
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 500 1414 500
Size: 914 3453
ScaI AGT'ACT
Cuts at: 664 3884 664
Size: 3220 1147
SfcI C'TryA_G
Cuts at: 33 496 1410 2095 3088 3279 33
Size: 463 914 685 993 191 1121
Fragments arranged by size:
1121 993 914 685 463 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 400 400
Size: 4367
SfoI GGC'GCC
Cuts at: 788 1022 1799 2047 788
Size: 234 777 248 3108
Fragments arranged by size:
3108 777 248 234
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SgrAI Cr'CCGG_yG
Cuts at: 175 175
Size: 4367
SmaI CCC'GGG
Cuts at: 958 1389 958
Size: 431 3936
SmlI C'TyrA_G
Cuts at: 577 1522 2437 2929 3191 577
Size: 945 915 492 262 1753
Fragments arranged by size:
1753 945 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 366 1420 1920 366
Size: 1054 500 2813
Fragments arranged by size:
2813 1054 500
StyI C'CwwG_G
Cuts at: 157 701 1501 1855 157
Size: 544 800 354 2669
Fragments arranged by size:
2669 800 544 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 621 1090 1950 2083 2542 2751 621
Size: 469 860 133 459 209 2237
Fragments arranged by size:
2237 860 469 459 209 133
TatI w'GTAC_w
Cuts at: 662 3882 662
Size: 3220 1147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 861 2164 861
Size: 1303 3064
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 956 1387 956
Size: 431 3936
ZraI GAC'GTC
Cuts at: 356 356
Size: 4367
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BfrBI BmtI BsaXI BsiWI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI