Restriction map for
pF1KB9016
(Circular) (Six-base) MAPSORT of: pF1KB9016.seq Check: 6060 from: 1 to: 3896
pF1KB9016 3896 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 257 827 934 257
Size: 570 107 3219
Fragments arranged by size:
3219 570 107
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaI G_GGCC'C
Cuts at: 716 716
Size: 3896
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 460 912 1326 1574 1609 460
Size: 452 414 248 35 2747
Fragments arranged by size:
2747 452 414 248 35
BanII G_rGCy'C
Cuts at: 716 910 1940 716
Size: 194 1030 2672
Fragments arranged by size:
2672 1030 194
BbeI G_GCGC'C
Cuts at: 464 1330 1578 464
Size: 866 248 2782
Fragments arranged by size:
2782 866 248
BbsI GAAGACnn'nnnn_
Cuts at: 889 889
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 673 1794 2555 3896 673
Size: 1121 761 1341 673
Fragments arranged by size:
1341 1121 761 673
BclI T'GATC_A
Cuts at: 183 1416 183
Size: 1233 2663
BglI GCCn_nnn'nGGC
Cuts at: 611 611
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 716 1521 1614 2670 716
Size: 805 93 1056 1942
Fragments arranged by size:
1942 1056 805 93
BmrI ACTGGGnnnn_n'
Cuts at: 518 1108 1189 1524 3668 518
Size: 590 81 335 2144 746
Fragments arranged by size:
2144 746 590 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 492 472
Size: 20 3876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1072 1951 2443 2741 485
Size: 587 879 492 298 1640
Fragments arranged by size:
1640 879 587 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 189 684 1249 189
Size: 495 565 2836
Fragments arranged by size:
2836 565 495
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 461 1327 1575 2277 3773 461
Size: 866 248 702 1496 584
Fragments arranged by size:
1496 866 702 584 248
BsaWI w'CCGG_w
Cuts at: 726 870 1085 1606 2284 2558 2705 3116 726
Size: 144 215 521 678 274 147 411 1506
Fragments arranged by size:
1506 678 521 411 274 215 147 144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 134 164 134
Size: 30 3866
BseYI C'CCAG_C
Cuts at: 416 553 1321 2079 2656 3152 416
Size: 137 768 758 577 496 1160
Fragments arranged by size:
1160 768 758 577 496 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 910 1688 1878 2670 910
Size: 778 190 792 2136
Fragments arranged by size:
2136 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 716 910 1521 1614 1688 1878 1940 2670 716
Size: 194 611 93 74 190 62 730 1942
Fragments arranged by size:
1942 730 611 194 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 317 335 3072 317
Size: 18 2737 1141
Fragments arranged by size:
2737 1141 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BssSI C'ACGA_G
Cuts at: 837 2167 2525 837
Size: 1330 358 2208
Fragments arranged by size:
2208 1330 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 674 716 107
Size: 567 42 3287
Fragments arranged by size:
3287 567 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 1481 1655 2046 2073 3418 1481
Size: 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 155 415 2104 2414 2560 3706 155
Size: 260 1689 310 146 1146 345
Fragments arranged by size:
1689 1146 345 310 260 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 492 1721 2153 2900 3168 472
Size: 20 1229 432 747 268 1200
Fragments arranged by size:
1229 1200 747 432 268 20
EcoO109I rG'GnC_Cy
Cuts at: 560 652 1035 560
Size: 92 383 3421
Fragments arranged by size:
3421 383 92
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 115 464 797 1330 1578 2600 3579 115
Size: 349 333 533 248 1022 979 432
Fragments arranged by size:
1022 979 533 432 349 333 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 503 535 733 765 1738 1768 1770
Size: 32 337 32 198 32 973 30 2
Cuts at: 1770 1800 134
Size: 30 2230
Fragments arranged by size:
2230 973 337 198 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 443 935 443
Size: 492 3404
KasI G'GCGC_C
Cuts at: 460 1326 1574 460
Size: 866 248 2782
Fragments arranged by size:
2782 866 248
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 172 1885 2012 172
Size: 1713 127 2056
Fragments arranged by size:
2056 1713 127
MspA1I CmG'CkG
Cuts at: 480 557 734 1007 1321 1681 2694 2939 480
Size: 77 177 273 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 360 314 273 245 177 77
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 461 1327 1575 461
Size: 866 248 2782
Fragments arranged by size:
2782 866 248
NcoI C'CATG_G
Cuts at: 107 674 716 107
Size: 567 42 3287
Fragments arranged by size:
3287 567 42
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 552 834 949 1449 2356 552
Size: 282 115 500 907 2092
Fragments arranged by size:
2092 907 500 282 115
PasI CC'CwG_GG
Cuts at: 709 709
Size: 3896
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PfoI T'CCnGG_A
Cuts at: 648 648
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PmlI CAC'GTG
Cuts at: 189 189
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 652 652
Size: 3896
PspOMI G'GGCC_C
Cuts at: 712 712
Size: 3896
PstI C_TGCA'G
Cuts at: 762 943 1628 762
Size: 181 685 3030
Fragments arranged by size:
3030 685 181
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 758 939 1624 2617 2808 33
Size: 725 181 685 993 191 1121
Fragments arranged by size:
1121 993 725 685 191 181
SfoI GGC'GCC
Cuts at: 462 1328 1576 462
Size: 866 248 2782
Fragments arranged by size:
2782 866 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 500 1051 1966 2458 2720 500
Size: 551 915 492 262 1676
Fragments arranged by size:
1676 915 551 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 107 290 674 716 770 1030 1384 107
Size: 183 384 42 54 260 354 2619
Fragments arranged by size:
2619 384 354 260 183 54 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI