Restriction map for
pF1KB9017
(Circular) (Six-base) MAPSORT of: pF1KB9017.seq Check: 743 from: 1 to: 4442
pF1KB9017 4442 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1182 1480 1182
Size: 298 4144
Acc65I G'GTAC_C
Cuts at: 1458 1458
Size: 4442
AclI AA'CG_TT
Cuts at: 4282 4282
Size: 4442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 736 2267 2699 3052 3714 287
Size: 449 1531 432 353 662 1015
Fragments arranged by size:
1531 1015 662 449 432 353
AflII C'TTAA_G
Cuts at: 1338 1338
Size: 4442
AflIII A'CryG_T
Cuts at: 1794 3594 3875 1794
Size: 1800 281 2361
Fragments arranged by size:
2361 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 855 1747 3185 855
Size: 892 1438 2112
Fragments arranged by size:
2112 1438 892
ApaLI G'TGCA_C
Cuts at: 414 1190 3280 414
Size: 776 2090 1576
Fragments arranged by size:
2090 1576 776
ApoI r'AATT_y
Cuts at: 640 910 934 1173 1446 3795 4051 640
Size: 270 24 239 273 2349 256 1031
Fragments arranged by size:
2349 1031 273 270 256 239 24
AseI AT'TA_AT
Cuts at: 20 796 820 20
Size: 776 24 3642
Fragments arranged by size:
3642 776 24
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
AvaI C'yCGr_G
Cuts at: 248 593 620 1462 248
Size: 345 27 842 3228
Fragments arranged by size:
3228 842 345 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 817 850 817
Size: 33 4409
BamHI G'GATC_C
Cuts at: 1467 1467
Size: 4442
BanI G'GyrC_C
Cuts at: 1458 1872 2120 2155 1458
Size: 414 248 35 3745
Fragments arranged by size:
3745 414 248 35
BanII G_rGCy'C
Cuts at: 1256 1456 2486 1256
Size: 200 1030 3212
Fragments arranged by size:
3212 1030 200
BbeI G_GCGC'C
Cuts at: 1876 2124 1876
Size: 248 4194
BbsI GAAGACnn'nnnn_
Cuts at: 1036 1036
Size: 4442
BciVI GTATCCnnnnn_n'
Cuts at: 845 1414 2340 3396 4442 845
Size: 569 926 1056 1046 845
Fragments arranged by size:
1056 1046 926 845 569
BclI T'GATC_A
Cuts at: 1962 1962
Size: 4442
BfrBI ATG'CAT
Cuts at: 928 928
Size: 4442
BglI GCCn_nnn'nGGC
Cuts at: 1220 1220
Size: 4442
BglII A'GATC_T
Cuts at: 1957 3994 1957
Size: 2037 2405
BlpI GC'TnA_GC
Cuts at: 1554 1554
Size: 4442
Bme1580I G_kGCm'C
Cuts at: 418 1194 2067 2160 3284 418
Size: 776 873 93 1124 1576
Fragments arranged by size:
1576 1124 873 776 93
BmrI ACTGGGnnnn_n'
Cuts at: 1654 1735 2070 4214 1654
Size: 81 335 2144 1882
Fragments arranged by size:
2144 1882 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 4442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1301 1063
Size: 238 4204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 687 1009 1618 2497 3211 3509 323
Size: 364 322 609 879 714 298 1256
Fragments arranged by size:
1256 879 714 609 364 322 298
BsaI GGTCTCn'nnnn_
Cuts at: 779 4146 779
Size: 3367 1075
BsaAI yAC'GTr
Cuts at: 1795 1795
Size: 4442
BsaBI GATnn'nnATC
Cuts at: 6 737 1956 3993 6
Size: 731 1219 2037 455
Fragments arranged by size:
2037 1219 731 455
BsaHI Gr'CG_yC
Cuts at: 1873 2121 2823 4319 1873
Size: 248 702 1496 1996
Fragments arranged by size:
1996 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1631 2152 2830 3241 3388 3662 1631
Size: 521 678 411 147 274 2411
Fragments arranged by size:
2411 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 445 159
Size: 286 4156
BseYI C'CCAG_C
Cuts at: 325 1867 2625 3290 3698 325
Size: 1542 758 665 408 1069
Fragments arranged by size:
1542 1069 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1984 2030 3260 104
Size: 1880 46 1230 1286
Fragments arranged by size:
1880 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 418 1194 1256 1362 1456 2234 2424 3284 418
Size: 776 62 106 94 778 190 860 1576
Fragments arranged by size:
1576 860 778 776 190 106 94 62
Bsp1286I G_dGCh'C
Cuts at: 418 1194 1256 1362 1456 2067 2160 2234 2424
Size: 776 62 106 94 611 93 74 190
Cuts at: 2424 2486 3284 418
Size: 62 798 1576
Fragments arranged by size:
1576 798 776 611 190 106 94 93
74 62 62
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1492 1706 2008 2389 1492
Size: 214 302 381 3545
Fragments arranged by size:
3545 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2734 2788 4272 4437 50
Size: 2684 54 1484 165 55
Fragments arranged by size:
2684 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 138 2354 138
Size: 2216 2226
BsrFI r'CCGG_y
Cuts at: 1121 2440 2621 1121
Size: 1319 181 2942
Fragments arranged by size:
2942 1319 181
BssSI C'ACGA_G
Cuts at: 2713 3421 2713
Size: 708 3734
BstAPI GCAn_nnn'nTGC
Cuts at: 523 1819 523
Size: 1296 3146
BstBI TT'CG_AA
Cuts at: 1171 2803 1171
Size: 1632 2810
BstXI CCAn_nnnn'nTGG
Cuts at: 1164 1164
Size: 4442
BstYI r'GATC_y
Cuts at: 1 1467 1934 1957 2292 2538 2844 2856 2942
Size: 1466 467 23 335 246 306 12 86
Cuts at: 2942 3994 1
Size: 1052 449
Fragments arranged by size:
1466 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 789 107
Size: 682 3760
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2392 2573 2736 2392
Size: 181 163 4098
Fragments arranged by size:
4098 181 163
BtsI GCAGTG_nn'
Cuts at: 244 1117 1685 244
Size: 873 568 3001
Fragments arranged by size:
3001 873 568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 869 904 869
Size: 35 4407
DraI TTT'AAA
Cuts at: 1441 1441
Size: 4442
DrdI GACnn_nn'nnGTC
Cuts at: 2148 3492 2148
Size: 1344 3098
EaeI y'GGCC_r
Cuts at: 602 2027 2201 2592 2619 3964 602
Size: 1425 174 391 27 1345 1080
Fragments arranged by size:
1425 1345 1080 391 174 27
EagI C'GGCC_G
Cuts at: 2027 2027
Size: 4442
EarI CTCTTCn'nnn_
Cuts at: 1037 2465 2675 1037
Size: 1428 210 2804
Fragments arranged by size:
2804 1428 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1108 2650 3392 3538 4252 1108
Size: 1542 742 146 714 1298
Fragments arranged by size:
1542 1298 742 714 146
EcoICRI GAG'CTC
Cuts at: 1254 1454 1254
Size: 200 4242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 736 1063 1301 2267 2699 3052 3714 287
Size: 449 327 238 966 432 353 662 1015
Fragments arranged by size:
1015 966 662 449 432 353 327 238
EcoNI CCTnn'n_nnAGG
Cuts at: 158 158
Size: 4442
EcoO109I rG'GnC_Cy
Cuts at: 852 1581 852
Size: 729 3713
EcoRI G'AATT_C
Cuts at: 1173 1446 1173
Size: 273 4169
EcoRV GAT'ATC
Cuts at: 735 735
Size: 4442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1066 2461 1066
Size: 1395 3047
FspI TGC'GCA
Cuts at: 2223 2223
Size: 4442
HaeII r_GCGC'y
Cuts at: 1050 1876 2124 3354 4125 1050
Size: 826 248 1230 771 1367
Fragments arranged by size:
1367 1230 826 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1349 1381 2284 2314 2316 2346 1349
Size: 32 903 30 2 30 3445
Fragments arranged by size:
3445 903 32 30 30 2
HincII GTy'rAC
Cuts at: 1481 1481
Size: 4442
KasI G'GCGC_C
Cuts at: 1872 2120 1872
Size: 248 4194
KpnI G_GTAC'C
Cuts at: 1462 1462
Size: 4442
MluI A'CGCG_T
Cuts at: 3875 3875
Size: 4442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3201 3385 3201
Size: 184 4258
MscI TGG'CCA
Cuts at: 2203 2203
Size: 4442
MslI CAynn'nnrTG
Cuts at: 287 1162 2431 2558 287
Size: 875 1269 127 2171
Fragments arranged by size:
2171 1269 875 127
MspA1I CmG'CkG
Cuts at: 329 1553 1867 2227 3011 3256 329
Size: 1224 314 360 784 245 1515
Fragments arranged by size:
1515 1224 784 360 314 245
NaeI GCC'GGC
Cuts at: 1123 2623 1123
Size: 1500 2942
NarI GG'CG_CC
Cuts at: 1873 2121 1873
Size: 248 4194
NcoI C'CATG_G
Cuts at: 107 789 107
Size: 682 3760
NgoMIV G'CCGG_C
Cuts at: 1121 2621 1121
Size: 1500 2942
NsiI A_TGCA'T
Cuts at: 930 930
Size: 4442
NspI r_CATG'y
Cuts at: 615 712 1495 1995 3598 615
Size: 97 783 500 1603 1459
Fragments arranged by size:
1603 1459 783 500 97
PciI A'CATG_T
Cuts at: 3594 3594
Size: 4442
PflMI CCAn_nnn'nTGG
Cuts at: 930 930
Size: 4442
PfoI T'CCnGG_A
Cuts at: 848 1088 848
Size: 240 4202
PmeI GTTT'AAAC
Cuts at: 1441 1441
Size: 4442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 4410
PpuMI rG'GwC_Cy
Cuts at: 852 852
Size: 4442
PsiI TTA'TAA
Cuts at: 781 781
Size: 4442
PspXI vC'TCGA_Gb
Cuts at: 248 620 248
Size: 372 4070
PstI C_TGCA'G
Cuts at: 1489 2174 1489
Size: 685 3757
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4442
PvuII CAG'CTG
Cuts at: 329 1867 2227 329
Size: 1538 360 2544
Fragments arranged by size:
2544 1538 360
RsrII CG'GwC_CG
Cuts at: 2637 2637
Size: 4442
SacI G_AGCT'C
Cuts at: 1256 1456 1256
Size: 200 4242
SalI G'TCGA_C
Cuts at: 1479 1479
Size: 4442
SapI GCTCTTCn'nnn_
Cuts at: 1037 2465 2675 1037
Size: 1428 210 2804
Fragments arranged by size:
2804 1428 210
SbfI CC_TGCA'GG
Cuts at: 1489 1489
Size: 4442
ScaI AGT'ACT
Cuts at: 3959 3959
Size: 4442
SfcI C'TryA_G
Cuts at: 33 551 1485 2170 3138 3329 33
Size: 518 934 685 968 191 1146
Fragments arranged by size:
1146 968 934 685 518 191
SfoI GGC'GCC
Cuts at: 1874 2122 1874
Size: 248 4194
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
SmaI CCC'GGG
Cuts at: 1464 1464
Size: 4442
SmlI C'TyrA_G
Cuts at: 248 338 620 666 1024 1338 1597 2512 3226
Size: 90 282 46 358 314 259 915 714
Cuts at: 3226 3488 248
Size: 262 1202
Fragments arranged by size:
1202 915 714 358 314 282 262 259
90 46
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4442
SphI G_CATG'C
Cuts at: 1495 1995 1495
Size: 500 3942
StyI C'CwwG_G
Cuts at: 107 425 789 839 1576 1930 107
Size: 318 364 50 737 354 2619
Fragments arranged by size:
2619 737 364 354 318 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2025 2158 2617 2826 2025
Size: 133 459 209 3641
Fragments arranged by size:
3641 459 209 133
TatI w'GTAC_w
Cuts at: 260 3957 260
Size: 3697 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 522 554 3959 3991 522
Size: 32 3405 32 973
Fragments arranged by size:
3405 973 32 32
Tth111I GACn'n_nGTC
Cuts at: 2239 2239
Size: 4442
XbaI T'CTAG_A
Cuts at: 1473 1473
Size: 4442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1937 114
Size: 1823 2619
XhoI C'TCGA_G
Cuts at: 248 620 248
Size: 372 4070
XmaI C'CCGG_G
Cuts at: 1462 1462
Size: 4442
XmnI GAAnn'nnTTC
Cuts at: 457 457
Size: 4442
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PmlI PshAI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI