Restriction map for
pF1KB9022
(Circular) (Six-base) MAPSORT of: pF1KB9022.seq Check: 9941 from: 1 to: 8249
pF1KB9022 8249 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5107 5107
Size: 8249
AccI GT'mk_AC
Cuts at: 5287 5287
Size: 8249
Acc65I G'GTAC_C
Cuts at: 5265 5265
Size: 8249
AclI AA'CG_TT
Cuts at: 8089 8089
Size: 8249
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2584 2998 3442 3478 3883 6074 6506 6859 7521
Size: 414 444 36 405 2191 432 353 662
Cuts at: 7521 2584
Size: 3312
Fragments arranged by size:
3312 2191 662 444 432 414 405 353
36
AflIII A'CryG_T
Cuts at: 5601 7401 7682 5601
Size: 1800 281 6168
Fragments arranged by size:
6168 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 8249
AleI CACnn'nnGTG
Cuts at: 2098 2098
Size: 8249
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4780 4812 4780
Size: 32 8217
AlwNI CAG_nnn'CTG
Cuts at: 2242 3745 3826 4015 4698 4874 5129 5219 5554
Size: 1503 81 189 683 176 255 90 335
Cuts at: 5554 6992 2242
Size: 1438 3499
Fragments arranged by size:
3499 1503 1438 683 335 255 189 176
90 81
ApaI G_GGCC'C
Cuts at: 310 491 554 590 1142 1218 1378 1903 1966
Size: 181 63 36 552 76 160 525 63
Cuts at: 1966 2053 2162 2261 2405 2663 3032 3997 4106
Size: 87 109 99 144 258 369 965 109
Cuts at: 4106 4151 4370 4378 4424 310
Size: 45 219 8 46 4135
Fragments arranged by size:
4135 965 552 525 369 258 219 181
160 144 109 109 99 87 76 63
63 46 45 36 8
ApaLI G'TGCA_C
Cuts at: 4511 7087 4511
Size: 2576 5673
ApoI r'AATT_y
Cuts at: 461 3399 5253 7602 7858 461
Size: 2938 1854 2349 256 852
Fragments arranged by size:
2938 2349 1854 852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8249
AvaI C'yCGr_G
Cuts at: 348 474 583 1106 1200 1254 1417 1810 2324
Size: 126 109 523 94 54 163 393 514
Cuts at: 2324 2449 2854 2926 4252 4270 4533 5146 5269
Size: 125 405 72 1326 18 263 613 123
Cuts at: 5269 348
Size: 3328
Fragments arranged by size:
3328 1326 613 523 514 405 393 263
163 126 125 123 109 94 72 54
18
BamHI G'GATC_C
Cuts at: 2329 2365 3689 3733 5274 2329
Size: 36 1324 44 1541 5304
Fragments arranged by size:
5304 1541 1324 44 36
BanI G'GyrC_C
Cuts at: 451 1186 2203 3325 3436 3715 3942 3960 4246
Size: 735 1017 1122 111 279 227 18 286
Cuts at: 4246 4350 5095 5265 5679 5927 5962 451
Size: 104 745 170 414 248 35 2738
Fragments arranged by size:
2738 1122 1017 745 735 414 286 279
248 227 170 111 104 35 18
BanII G_rGCy'C
Cuts at: 310 393 491 554 590 744 1142 1218 1378
Size: 83 98 63 36 154 398 76 160
Cuts at: 1378 1424 1518 1700 1853 1903 1966 2053 2162
Size: 46 94 182 153 50 63 87 109
Cuts at: 2162 2261 2405 2445 2646 2663 3032 3657 3864
Size: 99 144 40 201 17 369 625 207
Cuts at: 3864 3892 3997 4062 4106 4151 4269 4370 4378
Size: 28 105 65 44 45 118 101 8
Cuts at: 4378 4407 4424 4534 4613 4714 5167 5263 6293
Size: 29 17 110 79 101 453 96 1030
Cuts at: 6293 310
Size: 2266
Fragments arranged by size:
2266 1030 625 453 398 369 207 201
182 160 154 153 144 118 110 109
105 101 101 99 98 96 94 87
83 79 76 65 63 63 50 46
45 44 40 36 29 28 17 17
8
BbeI G_GCGC'C
Cuts at: 3964 5683 5931 3964
Size: 1719 248 6282
Fragments arranged by size:
6282 1719 248
BbsI GAAGACnn'nnnn_
Cuts at: 1582 3417 1582
Size: 1835 6414
BciVI GTATCCnnnnn_n'
Cuts at: 3109 3145 4642 6147 7203 8249 3109
Size: 36 1497 1505 1056 1046 3109
Fragments arranged by size:
3109 1505 1497 1056 1046 36
BclI T'GATC_A
Cuts at: 5769 5769
Size: 8249
BglI GCCn_nnn'nGGC
Cuts at: 585 1082 1676 1874 1985 1994 2057 2354 2400
Size: 497 594 198 111 9 63 297 46
Cuts at: 2400 2576 2603 2667 2747 3105 3189 3350 4625
Size: 176 27 64 80 358 84 161 1275
Cuts at: 4625 585
Size: 4209
Fragments arranged by size:
4209 1275 594 497 358 297 198 176
161 111 84 80 64 63 46 27
9
BglII A'GATC_T
Cuts at: 5764 7801 5764
Size: 2037 6212
BlpI GC'TnA_GC
Cuts at: 2444 5361 2444
Size: 2917 5332
Bme1580I G_kGCm'C
Cuts at: 310 491 554 590 1142 1218 1378 1811 1903
Size: 181 63 36 552 76 160 433 92
Cuts at: 1903 1966 2053 2162 2261 2405 2663 3032 3330
Size: 63 87 109 99 144 258 369 298
Cuts at: 3330 3439 3945 3997 4106 4151 4251 4353 4370
Size: 109 506 52 109 45 100 102 17
Cuts at: 4370 4378 4424 4515 4621 5874 5967 7091 310
Size: 8 46 91 106 1253 93 1124 1468
Fragments arranged by size:
1468 1253 1124 552 506 433 369 298
258 181 160 144 109 109 109 106
102 100 99 93 92 91 87 76
63 63 52 46 45 36 17 8
BmgBI CAC'GTC
Cuts at: 286 420 286
Size: 134 8115
BmrI ACTGGGnnnn_n'
Cuts at: 1984 3587 5461 5542 5877 8021 1984
Size: 1603 1874 81 335 2144 2212
Fragments arranged by size:
2212 2144 1874 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6616 6616
Size: 8249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 728 1391 1532 1889 1934 2033 2447 2603
Size: 42 663 141 357 45 99 414 156
Cuts at: 2603 3545 3569 3848 4046 4391 4662 686
Size: 942 24 279 198 345 271 4273
Fragments arranged by size:
4273 942 663 414 357 345 279 271
198 156 141 99 45 42 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1684 5170 5425 6304 7018 7316 1053
Size: 631 3486 255 879 714 298 1986
Fragments arranged by size:
3486 1986 879 714 631 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 110 744 1124 1244 1340 1655 2764 2916 3752
Size: 634 380 120 96 315 1109 152 836
Cuts at: 3752 3845 4135 4223 7953 110
Size: 93 290 88 3730 406
Fragments arranged by size:
3730 1109 836 634 406 380 315 290
152 120 96 93 88
BsaAI yAC'GTr
Cuts at: 5602 5602
Size: 8249
BsaBI GATnn'nnATC
Cuts at: 6 1085 1295 5763 7800 6
Size: 1079 210 4468 2037 455
Fragments arranged by size:
4468 2037 1079 455 210
BsaHI Gr'CG_yC
Cuts at: 1796 2018 3961 5680 5928 6630 8126 1796
Size: 222 1943 1719 248 702 1496 1919
Fragments arranged by size:
1943 1919 1719 1496 702 248 222
BsaWI w'CCGG_w
Cuts at: 5438 5959 6637 7048 7195 7469 5438
Size: 521 678 411 147 274 6218
Fragments arranged by size:
6218 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 564 594 1664 1694 2718 2748 3567 3597 4600
Size: 30 1070 30 1024 30 819 30 1003
Cuts at: 4600 4630 564
Size: 30 4183
Fragments arranged by size:
4183 1070 1024 1003 819 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 1317 2789 652
Size: 665 1472 6112
Fragments arranged by size:
6112 1472 665
BseYI C'CCAG_C
Cuts at: 162 1546 3603 4417 4944 4948 5674 6432 7097
Size: 1384 2057 814 527 4 726 758 665
Cuts at: 7097 7505 162
Size: 408 906
Fragments arranged by size:
2057 1384 906 814 758 726 665 527
408 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2360
Size: 8249
BsiEI CG_ry'CG
Cuts at: 104 705 5791 5837 7067 104
Size: 601 5086 46 1230 1286
Fragments arranged by size:
5086 1286 1230 601 46
BsiHKAI G_wGCw'C
Cuts at: 744 1728 2445 2646 3657 3864 4062 4515 5263
Size: 984 717 201 1011 207 198 453 748
Cuts at: 5263 6041 6231 7091 744
Size: 778 190 860 1902
Fragments arranged by size:
1902 1011 984 860 778 748 717 453
207 201 198 190
BsmI GAATG_Cn'
Cuts at: 1558 2846 3149 5083 1558
Size: 1288 303 1934 4724
Fragments arranged by size:
4724 1934 1288 303
BsmBI CGTCTCn'nnnn_
Cuts at: 2009 2270 4997 2009
Size: 261 2727 5261
Fragments arranged by size:
5261 2727 261
Bsp1286I G_dGCh'C
Cuts at: 310 393 491 554 590 744 1142 1218 1378
Size: 83 98 63 36 154 398 76 160
Cuts at: 1378 1424 1518 1700 1728 1811 1853 1903 1966
Size: 46 94 182 28 83 42 50 63
Cuts at: 1966 2053 2162 2261 2405 2445 2646 2663 3032
Size: 87 109 99 144 40 201 17 369
Cuts at: 3032 3330 3439 3657 3864 3892 3945 3997 4062
Size: 298 109 218 207 28 53 52 65
Cuts at: 4062 4106 4151 4251 4269 4353 4370 4378 4407
Size: 44 45 100 18 84 17 8 29
Cuts at: 4407 4424 4515 4534 4613 4621 4714 5167 5263
Size: 17 91 19 79 8 93 453 96
Cuts at: 5263 5874 5967 6041 6231 6293 7091 310
Size: 611 93 74 190 62 798 1468
Fragments arranged by size:
1468 798 611 453 398 369 298 218
207 201 190 182 160 154 144 109
109 100 99 98 96 94 93 93
91 87 84 83 83 79 76 74
65 63 63 62 53 52 50 46
45 44 42 40 36 29 28 28
19 18 17 17 17 8 8
BspHI T'CATG_A
Cuts at: 6681 6681
Size: 8249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1161 2272 5107 5299 5513 5815 6196 1161
Size: 1111 2835 192 214 302 381 3214
Fragments arranged by size:
3214 2835 1111 381 302 214 192
BsrBI CCG'CTC
Cuts at: 50 1782 3432 6541 6595 8079 8244 50
Size: 1732 1650 3109 54 1484 165 55
Fragments arranged by size:
3109 1732 1650 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5088 6161 5088
Size: 1073 7176
BsrFI r'CCGG_y
Cuts at: 133 2020 5191 6247 6428 133
Size: 1887 3171 1056 181 1954
Fragments arranged by size:
3171 1954 1887 1056 181
BsrGI T'GTAC_A
Cuts at: 1561 1561
Size: 8249
BssHII G'CGCG_C
Cuts at: 123 123
Size: 8249
BssSI C'ACGA_G
Cuts at: 6520 7228 6520
Size: 708 7541
BstAPI GCAn_nnn'nTGC
Cuts at: 1186 5626 1186
Size: 4440 3809
BstBI TT'CG_AA
Cuts at: 6610 6610
Size: 8249
BstEII G'GTnAC_C
Cuts at: 1021 1195 3793 1021
Size: 174 2598 5477
Fragments arranged by size:
5477 2598 174
BstXI CCAn_nnnn'nTGG
Cuts at: 1532 4952 1532
Size: 3420 4829
BstYI r'GATC_y
Cuts at: 1 2329 2365 3689 3733 5274 5741 5764 6099
Size: 2328 36 1324 44 1541 467 23 335
Cuts at: 6099 6345 6651 6663 6749 7801 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2328 1541 1324 1052 467 449 335 306
246 86 44 36 23 12
BtgI C'CryG_G
Cuts at: 107 176 1518 2455 3684 3693 3753 4838 5091
Size: 69 1342 937 1229 9 60 1085 253
Cuts at: 5091 107
Size: 3265
Fragments arranged by size:
3265 1342 1229 1085 937 253 69 60
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 876 1859 4884 4890 6199 6380 6543 876
Size: 983 3025 6 1309 181 163 2582
Fragments arranged by size:
3025 2582 1309 983 181 163 6
BtsI GCAGTG_nn'
Cuts at: 255 625 951 2348 4202 4814 4938 5492 255
Size: 370 326 1397 1854 612 124 554 3012
Fragments arranged by size:
3012 1854 1397 612 554 370 326 124
DraI TTT'AAA
Cuts at: 2826 3006 5248 2826
Size: 180 2242 5827
Fragments arranged by size:
5827 2242 180
DraIII CAC_nnn'GTG
Cuts at: 3949 3949
Size: 8249
DrdI GACnn_nn'nnGTC
Cuts at: 728 3226 5955 7299 728
Size: 2498 2729 1344 1678
Fragments arranged by size:
2729 2498 1678 1344
EaeI y'GGCC_r
Cuts at: 131 702 1668 2595 2649 3195 3646 4500 4841
Size: 571 966 927 54 546 451 854 341
Cuts at: 4841 4895 5016 5834 6008 6399 6426 7771 131
Size: 54 121 818 174 391 27 1345 609
Fragments arranged by size:
1345 966 927 854 818 609 571 546
451 391 341 174 121 54 54 27
EagI C'GGCC_G
Cuts at: 702 5834 702
Size: 5132 3117
EarI CTCTTCn'nnn_
Cuts at: 234 1473 4028 4425 6272 6482 234
Size: 1239 2555 397 1847 210 2001
Fragments arranged by size:
2555 2001 1847 1239 397 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3624 4971 5163 6457 7199 7345 8059 3624
Size: 1347 192 1294 742 146 714 3814
Fragments arranged by size:
3814 1347 1294 742 714 192 146
EcoICRI GAG'CTC
Cuts at: 742 2443 2644 3655 3862 4060 5261 742
Size: 1701 201 1011 207 198 1201 3730
Fragments arranged by size:
3730 1701 1201 1011 207 201 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 728 1391 1532 1889 1934 2033 2447 2584
Size: 42 663 141 357 45 99 414 137
Cuts at: 2584 2603 2998 3442 3478 3545 3569 3848 3883
Size: 19 395 444 36 67 24 279 35
Cuts at: 3883 4046 4391 4662 6074 6506 6859 7521 686
Size: 163 345 271 1412 432 353 662 1414
Fragments arranged by size:
1414 1412 663 662 444 432 414 395
357 353 345 279 271 163 141 137
99 67 45 42 36 35 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 275 275
Size: 8249
EcoO109I rG'GnC_Cy
Cuts at: 307 406 550 551 587 694 853 1093 1111
Size: 99 144 1 36 107 159 240 18
Cuts at: 1111 1138 1139 1214 1267 1327 1374 1402 1411
Size: 27 1 75 53 60 47 28 9
Cuts at: 1411 1606 1876 1899 1900 1911 1963 2050 2069
Size: 195 270 23 1 11 52 87 19
Cuts at: 2069 2158 2185 2194 2257 2302 2320 2374 2402
Size: 89 27 9 63 45 18 54 28
Cuts at: 2402 2418 2515 2587 2659 2660 2715 2849 3021
Size: 16 97 72 72 1 55 134 172
Cuts at: 3021 3029 3097 3535 3775 3823 3993 4103 4147
Size: 8 68 438 240 48 170 110 44
Cuts at: 4147 4210 4219 4321 4339 4366 4367 4374 4375
Size: 63 9 102 18 27 1 7 1
Cuts at: 4375 4421 4474 4695 4890 5388 307
Size: 46 53 221 195 498 3168
Fragments arranged by size:
3168 498 438 270 240 240 221 195
195 172 170 159 144 134 110 107
102 99 97 89 87 75 72 72
68 63 63 60 55 54 53 53
52 48 47 46 45 44 36 28
28 27 27 27 23 19 18 18
18 16 11 9 9 9 8 7
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 461 5253 461
Size: 4792 3457
EcoRV GAT'ATC
Cuts at: 652 652
Size: 8249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3483 5142 6268 3483
Size: 1659 1126 5464
Fragments arranged by size:
5464 1659 1126
FseI GG_CCGG'CC
Cuts at: 137 5195 137
Size: 5058 3191
FspI TGC'GCA
Cuts at: 6030 6030
Size: 8249
HaeII r_GCGC'y
Cuts at: 2735 3964 5683 5931 7161 7932 2735
Size: 1229 1719 248 1230 771 3052
Fragments arranged by size:
3052 1719 1230 1229 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2860 2892 3567 3599 4305 4337 6091 6121 6123
Size: 32 675 32 706 32 1754 30 2
Cuts at: 6123 6153 2860
Size: 30 4956
Fragments arranged by size:
4956 1754 706 675 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 5288 5288
Size: 8249
KasI G'GCGC_C
Cuts at: 3960 5679 5927 3960
Size: 1719 248 6282
Fragments arranged by size:
6282 1719 248
KpnI G_GTAC'C
Cuts at: 5269 5269
Size: 8249
MluI A'CGCG_T
Cuts at: 7682 7682
Size: 8249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 717 2094 3953 4329 7008 7192 717
Size: 1377 1859 376 2679 184 1774
Fragments arranged by size:
2679 1859 1774 1377 376 184
MscI TGG'CCA
Cuts at: 2651 4502 5018 6010 2651
Size: 1851 516 992 4890
Fragments arranged by size:
4890 1851 992 516
MslI CAynn'nnrTG
Cuts at: 2098 6238 6365 2098
Size: 4140 127 3982
Fragments arranged by size:
4140 3982 127
MspA1I CmG'CkG
Cuts at: 1520 3695 4824 4874 4948 5042 5093 5219 5360
Size: 2175 1129 50 74 94 51 126 141
Cuts at: 5360 5674 6034 6818 7063 1520
Size: 314 360 784 245 2706
Fragments arranged by size:
2706 2175 1129 784 360 314 245 141
126 94 74 51 50
NaeI GCC'GGC
Cuts at: 135 2022 5193 6430 135
Size: 1887 3171 1237 1954
Fragments arranged by size:
3171 1954 1887 1237
NarI GG'CG_CC
Cuts at: 3961 5680 5928 3961
Size: 1719 248 6282
Fragments arranged by size:
6282 1719 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 8249
NgoMIV G'CCGG_C
Cuts at: 133 2020 5191 6428 133
Size: 1887 3171 1237 1954
Fragments arranged by size:
3171 1954 1887 1237
NspI r_CATG'y
Cuts at: 718 2907 5302 5802 7405 718
Size: 2189 2395 500 1603 1562
Fragments arranged by size:
2395 2189 1603 1562 500
PasI CC'CwG_GG
Cuts at: 312 491 528 709 1135 1210 1492 1684 1693
Size: 179 37 181 426 75 282 192 9
Cuts at: 1693 2261 2308 2407 2719 3025 3157 3175 3259
Size: 568 47 99 312 306 132 18 84
Cuts at: 3259 3331 3505 4072 4107 4151 4326 4345 4371
Size: 72 174 567 35 44 175 19 26
Cuts at: 4371 312
Size: 4190
Fragments arranged by size:
4190 568 567 426 312 306 282 192
181 179 175 174 132 99 84 75
72 47 44 37 35 26 19 18
9
PciI A'CATG_T
Cuts at: 7401 7401
Size: 8249
PflMI CCAn_nnn'nTGG
Cuts at: 4085 4698 5025 4085
Size: 613 327 7309
Fragments arranged by size:
7309 613 327
PfoI T'CCnGG_A
Cuts at: 465 1008 1407 1593 1728 1923 1950 2502 2619
Size: 543 399 186 135 195 27 552 117
Cuts at: 2619 2808 3540 3675 3729 4026 4062 465
Size: 189 732 135 54 297 36 4652
Fragments arranged by size:
4652 732 552 543 399 297 195 189
186 135 135 117 54 36 27
PmeI GTTT'AAAC
Cuts at: 5248 5248
Size: 8249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6695 6727 6695
Size: 32 8217
PpuMI rG'GwC_Cy
Cuts at: 406 694 853 1111 1267 1327 1411 1606 1911
Size: 288 159 258 156 60 84 195 305
Cuts at: 1911 2069 2185 2302 2320 2374 2587 2715 2849
Size: 158 116 117 18 54 213 128 134
Cuts at: 2849 3021 4219 4321 4474 4695 406
Size: 172 1198 102 153 221 3960
Fragments arranged by size:
3960 1198 305 288 258 221 213 195
172 159 158 156 153 134 128 117
116 102 84 60 54 18
PshAI GACnn'nnGTC
Cuts at: 539 2282 3526 539
Size: 1743 1244 5262
Fragments arranged by size:
5262 1743 1244
PspOMI G'GGCC_C
Cuts at: 306 487 550 586 1138 1214 1374 1899 1962
Size: 181 63 36 552 76 160 525 63
Cuts at: 1962 2049 2158 2257 2401 2659 3028 3993 4102
Size: 87 109 99 144 258 369 965 109
Cuts at: 4102 4147 4366 4374 4420 306
Size: 45 219 8 46 4135
Fragments arranged by size:
4135 965 552 525 369 258 219 181
160 144 109 109 99 87 76 63
63 46 45 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1929 1961 1929
Size: 32 8217
PstI C_TGCA'G
Cuts at: 359 5296 5981 359
Size: 4937 685 2627
Fragments arranged by size:
4937 2627 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8249
PvuII CAG'CTG
Cuts at: 4948 5042 5674 6034 4948
Size: 94 632 360 7163
Fragments arranged by size:
7163 632 360 94
RsrII CG'GwC_CG
Cuts at: 6444 6444
Size: 8249
SacI G_AGCT'C
Cuts at: 744 2445 2646 3657 3864 4062 5263 744
Size: 1701 201 1011 207 198 1201 3730
Fragments arranged by size:
3730 1701 1201 1011 207 201 198
SacII CC_GC'GG
Cuts at: 1521 3696 5094 1521
Size: 2175 1398 4676
Fragments arranged by size:
4676 2175 1398
SalI G'TCGA_C
Cuts at: 5286 5286
Size: 8249
SanDI GG'GwC_CC
Cuts at: 853 1111 1327 1911 2069 2715 2849 3021 4219
Size: 258 216 584 158 646 134 172 1198
Cuts at: 4219 4321 853
Size: 102 4781
Fragments arranged by size:
4781 1198 646 584 258 216 172 158
134 102
SapI GCTCTTCn'nnn_
Cuts at: 1473 4028 6272 6482 1473
Size: 2555 2244 210 3240
Fragments arranged by size:
3240 2555 2244 210
SbfI CC_TGCA'GG
Cuts at: 5296 5296
Size: 8249
ScaI AGT'ACT
Cuts at: 7766 7766
Size: 8249
SexAI A'CCwGG_T
Cuts at: 3450 3666 3945 4008 4389 5240 3450
Size: 216 279 63 381 851 6459
Fragments arranged by size:
6459 851 381 279 216 63
SfcI C'TryA_G
Cuts at: 33 355 5292 5977 6945 7136 33
Size: 322 4937 685 968 191 1146
Fragments arranged by size:
4937 1146 968 685 322 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 585 1676 1874 1985 1994 2057 2354 2400 2576
Size: 1091 198 111 9 63 297 46 176
Cuts at: 2576 2603 585
Size: 27 6231
Fragments arranged by size:
6231 1091 297 198 176 111 63 46
27 9
SfoI GGC'GCC
Cuts at: 3962 5681 5929 3962
Size: 1719 248 6282
Fragments arranged by size:
6282 1719 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8249
SmaI CCC'GGG
Cuts at: 350 476 585 1108 1202 1256 1419 1812 2451
Size: 126 109 523 94 54 163 393 639
Cuts at: 2451 2856 2928 4254 4272 4535 5271 350
Size: 405 72 1326 18 263 736 3328
Fragments arranged by size:
3328 1326 736 639 523 405 393 263
163 126 109 94 72 54 18
SmlI C'TyrA_G
Cuts at: 1032 1699 5185 5404 6319 7033 7295 1032
Size: 667 3486 219 915 714 262 1986
Fragments arranged by size:
3486 1986 915 714 667 262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8249
SphI G_CATG'C
Cuts at: 2907 5302 5802 2907
Size: 2395 500 5354
Fragments arranged by size:
5354 2395 500
SrfI GCCC'GGGC
Cuts at: 2451 4535 2451
Size: 2084 6165
StuI AGG'CCT
Cuts at: 1076 1310 2177 2213 2498 2579 2606 3344 3419
Size: 234 867 36 285 81 27 738 75
Cuts at: 3419 4116 1076
Size: 697 5209
Fragments arranged by size:
5209 867 738 697 285 234 81 75
36 27
StyI C'CwwG_G
Cuts at: 107 410 591 1115 1218 1331 1610 2162 2538
Size: 303 181 524 103 113 279 552 376
Cuts at: 2538 2663 2898 3138 3531 3719 3855 4214 4223
Size: 125 235 240 393 188 136 359 9
Cuts at: 4223 4425 5383 5737 107
Size: 202 958 354 2619
Fragments arranged by size:
2619 958 552 524 393 376 359 354
303 279 240 235 202 188 181 136
125 113 103 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 932 4940 5832 5965 6424 6633 932
Size: 4008 892 133 459 209 2548
Fragments arranged by size:
4008 2548 892 459 209 133
TatI w'GTAC_w
Cuts at: 926 1561 4661 4748 5120 7764 926
Size: 635 3100 87 372 2644 1411
Fragments arranged by size:
3100 2644 1411 635 372 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 564 596 1806 1838 2027 2059 2718 2750 4600
Size: 32 1210 32 189 32 659 32 1850
Cuts at: 4600 4632 7766 7798 564
Size: 32 3134 32 1015
Fragments arranged by size:
3134 1850 1210 1015 659 189 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 287 6046 287
Size: 5759 2490
XbaI T'CTAG_A
Cuts at: 5046 5280 5046
Size: 234 8015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 319 417 2340 2545 2905 3538 4114 4194 4432
Size: 98 1923 205 360 633 576 80 238
Cuts at: 4432 4495 4620 4836 5744 319
Size: 63 125 216 908 2824
Fragments arranged by size:
2824 1923 908 633 576 360 238 216
205 125 98 80 63
XmaI C'CCGG_G
Cuts at: 348 474 583 1106 1200 1254 1417 1810 2449
Size: 126 109 523 94 54 163 393 639
Cuts at: 2449 2854 2926 4252 4270 4533 5269 348
Size: 405 72 1326 18 263 736 3328
Fragments arranged by size:
3328 1326 736 639 523 405 393 263
163 126 109 94 72 54 18
XmnI GAAnn'nnTTC
Cuts at: 3463 3463
Size: 8249
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BspEI BstZ17I
Bsu36I ClaI CspCI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PspXI
SgrAI SnaBI SspI SwaI XhoI ZraI