Restriction map for
pF1KB9023
(Circular) (Six-base) MAPSORT of: pF1KB9023.seq Check: 4099 from: 1 to: 7238
pF1KB9023 7238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1921 2658 1921
Size: 737 6501
AccI GT'mk_AC
Cuts at: 1304 3360 4205 4276 1304
Size: 2056 845 71 4266
Fragments arranged by size:
4266 2056 845 71
Acc65I G'GTAC_C
Cuts at: 2480 3085 4254 2480
Size: 605 1169 5464
Fragments arranged by size:
5464 1169 605
AclI AA'CG_TT
Cuts at: 7078 7078
Size: 7238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 832 990 1445 1448 1703 1905 3475 3692
Size: 84 158 455 3 255 202 1570 217
Cuts at: 3692 4047 5063 5495 5848 6510 748
Size: 355 1016 432 353 662 1476
Fragments arranged by size:
1570 1476 1016 662 455 432 355 353
255 217 202 158 84 3
AfeI AGC'GCT
Cuts at: 3169 3169
Size: 7238
AflII C'TTAA_G
Cuts at: 1479 1479
Size: 7238
AflIII A'CryG_T
Cuts at: 2411 4590 6390 6671 2411
Size: 2179 1800 281 2978
Fragments arranged by size:
2978 2179 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 4033 4033
Size: 7238
AleI CACnn'nnGTG
Cuts at: 1759 2383 2414 3197 1759
Size: 624 31 783 5800
Fragments arranged by size:
5800 783 624 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2841 2960 3031 3334 3451 2841
Size: 119 71 303 117 6628
Fragments arranged by size:
6628 303 119 117 71
AlwNI CAG_nnn'CTG
Cuts at: 249 1936 2277 3637 4543 5981 249
Size: 1687 341 1360 906 1438 1506
Fragments arranged by size:
1687 1506 1438 1360 906 341
ApaI G_GGCC'C
Cuts at: 1237 2976 1237
Size: 1739 5499
ApaLI G'TGCA_C
Cuts at: 1747 2775 6076 1747
Size: 1028 3301 2909
Fragments arranged by size:
3301 2909 1028
ApoI r'AATT_y
Cuts at: 2079 3651 3905 4242 6591 6847 2079
Size: 1572 254 337 2349 256 2470
Fragments arranged by size:
2470 2349 1572 337 256 254
AseI AT'TA_AT
Cuts at: 20 20
Size: 7238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7238
AvaI C'yCGr_G
Cuts at: 633 776 1971 3209 4258 633
Size: 143 1195 1238 1049 3613
Fragments arranged by size:
3613 1238 1195 1049 143
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1330 1363 1330
Size: 33 7205
BamHI G'GATC_C
Cuts at: 890 1191 3816 4263 890
Size: 301 2625 447 3865
Fragments arranged by size:
3865 2625 447 301
BanI G'GyrC_C
Cuts at: 340 2061 2432 2480 2768 3085 3202 3325 3343
Size: 1721 371 48 288 317 117 123 18
Cuts at: 3343 3427 3481 4254 4668 4916 4951 340
Size: 84 54 773 414 248 35 2627
Fragments arranged by size:
2627 1721 773 414 371 317 288 248
123 117 84 54 48 35 18
BanII G_rGCy'C
Cuts at: 215 1237 1262 2976 3140 4252 5282 215
Size: 1022 25 1714 164 1112 1030 2171
Fragments arranged by size:
2171 1714 1112 1030 1022 164 25
BbeI G_GCGC'C
Cuts at: 4672 4920 4672
Size: 248 6990
BbsI GAAGACnn'nnnn_
Cuts at: 1517 1530 1517
Size: 13 7225
BbvCI CC'TCA_GC
Cuts at: 2006 2006
Size: 7238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 282 316 282
Size: 34 7204
BciVI GTATCCnnnnn_n'
Cuts at: 1152 5136 6192 7238 1152
Size: 3984 1056 1046 1152
Fragments arranged by size:
3984 1152 1056 1046
BclI T'GATC_A
Cuts at: 2639 3911 4758 2639
Size: 1272 847 5119
Fragments arranged by size:
5119 1272 847
BglI GCCn_nnn'nGGC
Cuts at: 577 1807 1877 577
Size: 1230 70 5938
Fragments arranged by size:
5938 1230 70
BglII A'GATC_T
Cuts at: 3786 4753 6790 3786
Size: 967 2037 4234
Fragments arranged by size:
4234 2037 967
BlpI GC'TnA_GC
Cuts at: 273 4350 273
Size: 4077 3161
Bme1580I G_kGCm'C
Cuts at: 343 1237 1751 2771 2779 2976 3230 3328 3430
Size: 894 514 1020 8 197 254 98 102
Cuts at: 3430 4863 4956 6080 343
Size: 1433 93 1124 1501
Fragments arranged by size:
1501 1433 1124 1020 894 514 254 197
102 98 93 8
BmrI ACTGGGnnnn_n'
Cuts at: 2949 3225 3634 4450 4531 4866 7010 2949
Size: 276 409 816 81 335 2144 3177
Fragments arranged by size:
3177 2144 816 409 335 276 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 507 5605 507
Size: 5098 2140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 3192 3208 4021 4125 917
Size: 2275 16 813 104 4030
Fragments arranged by size:
4030 2275 813 104 16
Bpu10I CC'TnA_GC
Cuts at: 2006 2032 2006
Size: 26 7212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2895 4036 4414 5293 6007 6305 2895
Size: 1141 378 879 714 298 3828
Fragments arranged by size:
3828 1141 879 714 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 956 1270 6942 956
Size: 314 5672 1252
Fragments arranged by size:
5672 1252 314
BsaAI yAC'GTr
Cuts at: 336 1544 1731 3225 4591 336
Size: 1208 187 1494 1366 2983
Fragments arranged by size:
2983 1494 1366 1208 187
BsaBI GATnn'nnATC
Cuts at: 6 4160 4752 6789 6
Size: 4154 592 2037 455
Fragments arranged by size:
4154 2037 592 455
BsaHI Gr'CG_yC
Cuts at: 4669 4917 5619 7115 4669
Size: 248 702 1496 4792
Fragments arranged by size:
4792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2382 4427 4948 5626 6037 6184 6458 2382
Size: 2045 521 678 411 147 274 3162
Fragments arranged by size:
3162 2045 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1376 1406 2107 2113 2137 2143 2670 2700 3078
Size: 30 701 6 24 6 527 30 378
Cuts at: 3078 3108 3357 3387 4101 4131 1376
Size: 30 249 30 714 30 4483
Fragments arranged by size:
4483 714 701 527 378 249 30 30
30 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 1087 1120 1837 1993 4122 622
Size: 465 33 717 156 2129 3738
Fragments arranged by size:
3738 2129 717 465 156 33
BseYI C'CCAG_C
Cuts at: 504 1287 1416 2073 2270 2692 3335 3974 4663
Size: 783 129 657 197 422 643 639 689
Cuts at: 4663 5421 6086 6494 504
Size: 758 665 408 1248
Fragments arranged by size:
1248 783 758 689 665 657 643 639
422 408 197 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 286 1593 2253 3340 184
Size: 102 1307 660 1087 4082
Fragments arranged by size:
4082 1307 1087 660 102
BsiEI CG_ry'CG
Cuts at: 104 136 3647 4780 4826 6056 104
Size: 32 3511 1133 46 1230 1286
Fragments arranged by size:
3511 1286 1230 1133 46 32
BsiHKAI G_wGCw'C
Cuts at: 215 1751 2688 2779 3194 4252 5030 5220 6080
Size: 1536 937 91 415 1058 778 190 860
Cuts at: 6080 215
Size: 1373
Fragments arranged by size:
1536 1373 1058 937 860 778 415 190
91
BsmI GAATG_Cn'
Cuts at: 2797 2797
Size: 7238
BsmBI CGTCTCn'nnnn_
Cuts at: 1975 3634 1975
Size: 1659 5579
Bsp1286I G_dGCh'C
Cuts at: 215 343 1237 1262 1751 2688 2771 2779 2976
Size: 128 894 25 489 937 83 8 197
Cuts at: 2976 3140 3194 3230 3328 3430 4252 4863 4956
Size: 164 54 36 98 102 822 611 93
Cuts at: 4956 5030 5220 5282 6080 215
Size: 74 190 62 798 1373
Fragments arranged by size:
1373 937 894 822 798 611 489 197
190 164 128 102 98 93 83 74
62 54 36 25 8
BspHI T'CATG_A
Cuts at: 3072 5670 3072
Size: 2598 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 1775 1921 2658 2833 3191 4288 4502 4804
Size: 1193 146 737 175 358 1097 214 302
Cuts at: 4804 5185 582
Size: 381 2635
Fragments arranged by size:
2635 1193 1097 737 381 358 302 214
175 146
BsrBI CCG'CTC
Cuts at: 50 3332 5530 5584 7068 7233 50
Size: 3282 2198 54 1484 165 55
Fragments arranged by size:
3282 2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1794 5150 1794
Size: 3356 3882
BsrFI r'CCGG_y
Cuts at: 135 2621 2758 5236 5417 135
Size: 2486 137 2478 181 1956
Fragments arranged by size:
2486 2478 1956 181 137
BsrGI T'GTAC_A
Cuts at: 4006 4006
Size: 7238
BssHII G'CGCG_C
Cuts at: 528 550 528
Size: 22 7216
BssSI C'ACGA_G
Cuts at: 450 5509 6217 450
Size: 5059 708 1471
Fragments arranged by size:
5059 1471 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1677 1936 4615 1677
Size: 259 2679 4300
Fragments arranged by size:
4300 2679 259
BstBI TT'CG_AA
Cuts at: 1446 5599 1446
Size: 4153 3085
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 3460 3540 3813 3999 2491
Size: 969 80 273 186 5730
Fragments arranged by size:
5730 969 273 186 80
BstYI r'GATC_y
Cuts at: 1 890 1191 3786 3816 4263 4730 4753 5088
Size: 889 301 2595 30 447 467 23 335
Cuts at: 5088 5334 5640 5652 5738 6790 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2595 1052 889 467 449 447 335 306
301 246 86 30 23 12
BstZ17I GTA'TAC
Cuts at: 3361 3361
Size: 7238
BtgI C'CryG_G
Cuts at: 107 191 278 456 2715 3270 3380 107
Size: 84 87 178 2259 555 110 3965
Fragments arranged by size:
3965 2259 555 178 110 87 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 408 2521 2777 3251 3792 5188 5369 5532 408
Size: 2113 256 474 541 1396 181 163 2114
Fragments arranged by size:
2114 2113 1396 541 474 256 181 163
BtsI GCAGTG_nn'
Cuts at: 506 1638 3271 4481 506
Size: 1132 1633 1210 3263
Fragments arranged by size:
3263 1633 1210 1132
DraI TTT'AAA
Cuts at: 1244 4237 1244
Size: 2993 4245
DraIII CAC_nnn'GTG
Cuts at: 1701 2926 1701
Size: 1225 6013
DrdI GACnn_nn'nnGTC
Cuts at: 3516 4944 6288 3516
Size: 1428 1344 4466
Fragments arranged by size:
4466 1428 1344
EaeI y'GGCC_r
Cuts at: 125 133 419 512 578 1119 1719 1808 3400
Size: 8 286 93 66 541 600 89 1592
Cuts at: 3400 4143 4823 4997 5388 5415 6760 125
Size: 743 680 174 391 27 1345 603
Fragments arranged by size:
1592 1345 743 680 603 600 541 391
286 174 93 89 66 27 8
EagI C'GGCC_G
Cuts at: 133 4823 133
Size: 4690 2548
EarI CTCTTCn'nnn_
Cuts at: 220 987 3781 5261 5471 220
Size: 767 2794 1480 210 1987
Fragments arranged by size:
2794 1987 1480 767 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 454 5446 6188 6334 7048 454
Size: 4992 742 146 714 644
Fragments arranged by size:
4992 742 714 644 146
EcoICRI GAG'CTC
Cuts at: 213 4250 213
Size: 4037 3201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 832 917 990 1445 1448 1703 1905 3192
Size: 84 85 73 455 3 255 202 1287
Cuts at: 3192 3208 3475 3692 4021 4047 4125 5063 5495
Size: 16 267 217 329 26 78 938 432
Cuts at: 5495 5848 6510 748
Size: 353 662 1476
Fragments arranged by size:
1476 1287 938 662 455 432 353 329
267 255 217 202 85 84 78 73
26 16 3
EcoO109I rG'GnC_Cy
Cuts at: 1233 1842 2972 3149 3771 4377 1233
Size: 609 1130 177 622 606 4094
Fragments arranged by size:
4094 1130 622 609 606 177
EcoRI G'AATT_C
Cuts at: 3651 3905 4242 3651
Size: 254 337 6647
Fragments arranged by size:
6647 337 254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3300 3792 4077 5257 3300
Size: 492 285 1180 5281
Fragments arranged by size:
5281 1180 492 285
FspI TGC'GCA
Cuts at: 2472 5019 2472
Size: 2547 4691
FspAI rTGC'GCAy
Cuts at: 2472 2472
Size: 7238
HaeII r_GCGC'y
Cuts at: 199 602 628 2943 3171 3746 4672 4920 6150
Size: 403 26 2315 228 575 926 248 1230
Cuts at: 6150 6921 199
Size: 771 516
Fragments arranged by size:
2315 1230 926 771 575 516 403 248
228 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 455 487 799 831 2458 2490 2779
Size: 32 167 32 312 32 1627 32 289
Cuts at: 2779 2811 2893 2925 3587 3619 3943 3975 4019
Size: 32 82 32 662 32 324 32 44
Cuts at: 4019 4051 4169 4201 5080 5110 5112 5142 256
Size: 32 118 32 879 30 2 30 2352
Fragments arranged by size:
2352 1627 879 662 324 312 289 167
118 82 44 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2863 4277 2863
Size: 1414 5824
KasI G'GCGC_C
Cuts at: 4668 4916 4668
Size: 248 6990
KpnI G_GTAC'C
Cuts at: 2484 3089 4258 2484
Size: 605 1169 5464
Fragments arranged by size:
5464 1169 605
MfeI C'AATT_G
Cuts at: 1862 1862
Size: 7238
MluI A'CGCG_T
Cuts at: 6671 6671
Size: 7238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 914 939 1550 2150 5997 6181 914
Size: 25 611 600 3847 184 1971
Fragments arranged by size:
3847 1971 611 600 184 25
MscI TGG'CCA
Cuts at: 580 1121 1721 4145 4999 580
Size: 541 600 2424 854 2819
Fragments arranged by size:
2819 2424 854 600 541
MslI CAynn'nnrTG
Cuts at: 1759 2383 2414 2540 2681 3197 3538 3811 5227
Size: 624 31 126 141 516 341 273 1416
Cuts at: 5227 5354 1759
Size: 127 3643
Fragments arranged by size:
3643 1416 624 516 341 273 141 127
126 31
MspA1I CmG'CkG
Cuts at: 193 952 973 2277 2735 4349 4663 5023 5807
Size: 759 21 1304 458 1614 314 360 784
Cuts at: 5807 6052 193
Size: 245 1379
Fragments arranged by size:
1614 1379 1304 784 759 458 360 314
245 21
NaeI GCC'GGC
Cuts at: 2760 5419 2760
Size: 2659 4579
NarI GG'CG_CC
Cuts at: 4669 4917 4669
Size: 248 6990
NcoI C'CATG_G
Cuts at: 107 2715 3380 107
Size: 2608 665 3965
Fragments arranged by size:
3965 2608 665
NgoMIV G'CCGG_C
Cuts at: 2758 5417 2758
Size: 2659 4579
NspI r_CATG'y
Cuts at: 1603 1774 2415 4291 4791 6394 1603
Size: 171 641 1876 500 1603 2447
Fragments arranged by size:
2447 1876 1603 641 500 171
PasI CC'CwG_GG
Cuts at: 374 993 1262 2968 374
Size: 619 269 1706 4644
Fragments arranged by size:
4644 1706 619 269
PciI A'CATG_T
Cuts at: 2411 6390 2411
Size: 3979 3259
PflMI CCAn_nnn'nTGG
Cuts at: 1953 1953
Size: 7238
PfoI T'CCnGG_A
Cuts at: 3725 3725
Size: 7238
PmeI GTTT'AAAC
Cuts at: 4237 4237
Size: 7238
PmlI CAC'GTG
Cuts at: 1731 1731
Size: 7238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5684 5716 5684
Size: 32 7206
PpuMI rG'GwC_Cy
Cuts at: 3771 3771
Size: 7238
PshAI GACnn'nnGTC
Cuts at: 2253 2253
Size: 7238
PspOMI G'GGCC_C
Cuts at: 1233 2972 1233
Size: 1739 5499
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2206 2238 2206
Size: 32 7206
PstI C_TGCA'G
Cuts at: 173 254 722 957 2820 3266 4203 4285 4970
Size: 81 468 235 1863 446 937 82 685
Cuts at: 4970 173
Size: 2441
Fragments arranged by size:
2441 1863 937 685 468 446 235 82
81
PvuI CG_AT'CG
Cuts at: 104 3647 104
Size: 3543 3695
PvuII CAG'CTG
Cuts at: 952 2277 2735 4663 5023 952
Size: 1325 458 1928 360 3167
Fragments arranged by size:
3167 1928 1325 458 360
RsrII CG'GwC_CG
Cuts at: 5433 5433
Size: 7238
SacI G_AGCT'C
Cuts at: 215 4252 215
Size: 4037 3201
SacII CC_GC'GG
Cuts at: 194 194
Size: 7238
SalI G'TCGA_C
Cuts at: 4275 4275
Size: 7238
SapI GCTCTTCn'nnn_
Cuts at: 220 5261 5471 220
Size: 5041 210 1987
Fragments arranged by size:
5041 1987 210
SbfI CC_TGCA'GG
Cuts at: 254 4285 254
Size: 4031 3207
ScaI AGT'ACT
Cuts at: 6755 6755
Size: 7238
SexAI A'CCwGG_T
Cuts at: 2225 2225
Size: 7238
SfcI C'TryA_G
Cuts at: 33 169 250 718 953 1016 2580 2816 3022
Size: 136 81 468 235 63 1564 236 206
Cuts at: 3022 3262 4199 4281 4966 5934 6125 33
Size: 240 937 82 685 968 191 1146
Fragments arranged by size:
1564 1146 968 937 685 468 240 236
235 206 191 136 82 81 63
SfoI GGC'GCC
Cuts at: 4670 4918 4670
Size: 248 6990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7238
SmaI CCC'GGG
Cuts at: 1973 3211 4260 1973
Size: 1238 1049 4951
Fragments arranged by size:
4951 1238 1049
SmlI C'TyrA_G
Cuts at: 1479 2910 4051 4393 5308 6022 6284 1479
Size: 1431 1141 342 915 714 262 2433
Fragments arranged by size:
2433 1431 1141 915 714 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7238
SphI G_CATG'C
Cuts at: 1603 1774 4291 4791 1603
Size: 171 2517 500 4050
Fragments arranged by size:
4050 2517 500 171
StuI AGG'CCT
Cuts at: 1871 1871
Size: 7238
StyI C'CwwG_G
Cuts at: 107 726 1187 2715 3380 4372 4726 107
Size: 619 461 1528 665 992 354 2619
Fragments arranged by size:
2619 1528 992 665 619 461 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 218 580 2284 3157 3393 4821 4954 5413 5622
Size: 362 1704 873 236 1428 133 459 209
Cuts at: 5622 218
Size: 1834
Fragments arranged by size:
1834 1704 1428 873 459 362 236 209
133
TatI w'GTAC_w
Cuts at: 3575 3707 3836 4006 6753 3575
Size: 132 129 170 2747 4060
Fragments arranged by size:
4060 2747 170 132 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1176 1208 1376 1408 1630 1662 1701 1733 3357
Size: 32 168 32 222 32 39 32 1624
Cuts at: 3357 3368 3389 3400 3705 3737 4101 4133 6755
Size: 11 21 11 305 32 364 32 2622
Cuts at: 6755 6787 1176
Size: 32 1627
Fragments arranged by size:
2622 1627 1624 364 305 222 168 39
32 32 32 32 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 940 2290 3721 5035 940
Size: 1350 1431 1314 3143
Fragments arranged by size:
3143 1431 1350 1314
XbaI T'CTAG_A
Cuts at: 4269 4269
Size: 7238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3097 4733 3097
Size: 1636 5602
XmaI C'CCGG_G
Cuts at: 1971 3209 4258 1971
Size: 1238 1049 4951
Fragments arranged by size:
4951 1238 1049
XmnI GAAnn'nnTTC
Cuts at: 649 1088 1439 1918 649
Size: 439 351 479 5969
Fragments arranged by size:
5969 479 439 351
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AloI AscI AvrII BfrBI BmgBI BmtI
BsiWI BspEI BstEII Bsu36I ClaI CspCI EcoNI EcoRV
FseI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PsiI PspXI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI