Restriction map for
pF1KB9025
(Circular) (Six-base) MAPSORT of: pF1KB9025.seq Check: 9597 from: 1 to: 4415
pF1KB9025 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 734 1453 734
Size: 719 3696
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 262 696 1225 1289 2240 2672 3025 3687
Size: 66 434 529 64 951 432 353 662
Cuts at: 3687 196
Size: 924
Fragments arranged by size:
951 924 662 529 434 432 353 66
64
AflIII A'CryG_T
Cuts at: 1767 3567 3848 1767
Size: 1800 281 2334
Fragments arranged by size:
2334 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 563 563
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 1720 3158 1720
Size: 1438 2977
ApaLI G'TGCA_C
Cuts at: 3253 3253
Size: 4415
ApoI r'AATT_y
Cuts at: 214 462 523 573 698 839 937 1419 3768
Size: 248 61 50 125 141 98 482 2349
Cuts at: 3768 4024 214
Size: 256 605
Fragments arranged by size:
2349 605 482 256 248 141 125 98
61 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 1435 1435
Size: 4415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1192 1225 1192
Size: 33 4382
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 116 1431 1845 2093 2128 116
Size: 1315 414 248 35 2403
Fragments arranged by size:
2403 1315 414 248 35
BanII G_rGCy'C
Cuts at: 1326 1429 2459 1326
Size: 103 1030 3282
Fragments arranged by size:
3282 1030 103
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbsI GAAGACnn'nnnn_
Cuts at: 513 1411 513
Size: 898 3517
BbvCI CC'TCA_GC
Cuts at: 112 515 112
Size: 403 4012
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4381
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3369 4415 2313
Size: 1056 1046 2313
Fragments arranged by size:
2313 1056 1046
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglII A'GATC_T
Cuts at: 561 1930 3967 561
Size: 1369 2037 1009
Fragments arranged by size:
2037 1369 1009
BlpI GC'TnA_GC
Cuts at: 1527 1527
Size: 4415
Bme1580I G_kGCm'C
Cuts at: 2040 2133 3257 2040
Size: 93 1124 3198
Fragments arranged by size:
3198 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1627 1708 2043 4187 1627
Size: 81 335 2144 1855
Fragments arranged by size:
2144 1855 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
Bpu10I CC'TnA_GC
Cuts at: 112 515 112
Size: 403 4012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 2470 3184 3482 1591
Size: 879 714 298 2524
Fragments arranged by size:
2524 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4119 4119
Size: 4415
BsaAI yAC'GTr
Cuts at: 1768 1768
Size: 4415
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 1846 2094 2796 4292 1846
Size: 248 702 1496 1969
Fragments arranged by size:
1969 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3214 3361 3635 1604
Size: 521 678 411 147 274 2384
Fragments arranged by size:
2384 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 287
Size: 30 4385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 311 434 1075 311
Size: 123 641 3651
Fragments arranged by size:
3651 641 123
BseYI C'CCAG_C
Cuts at: 394 1840 2598 3263 3671 394
Size: 1446 758 665 408 1138
Fragments arranged by size:
1446 1138 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3233 104
Size: 1853 46 1230 1286
Fragments arranged by size:
1853 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1223 1429 2207 2397 3257 1223
Size: 206 778 190 860 2381
Fragments arranged by size:
2381 860 778 206 190
Bsp1286I G_dGCh'C
Cuts at: 1223 1326 1429 2040 2133 2207 2397 2459 3257
Size: 103 103 611 93 74 190 62 798
Cuts at: 3257 1223
Size: 2381
Fragments arranged by size:
2381 798 611 190 103 103 93 74
62
BspHI T'CATG_A
Cuts at: 453 2847 453
Size: 2394 2021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 924 1465 1679 1981 2362 924
Size: 541 214 302 381 2977
Fragments arranged by size:
2977 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 2413 2594 2413
Size: 181 4234
BssSI C'ACGA_G
Cuts at: 1187 2686 3394 1187
Size: 1499 708 2208
Fragments arranged by size:
2208 1499 708
BstAPI GCAn_nnn'nTGC
Cuts at: 784 1792 784
Size: 1008 3407
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstEII G'GTnAC_C
Cuts at: 745 850 1363 745
Size: 105 513 3797
Fragments arranged by size:
3797 513 105
BstYI r'GATC_y
Cuts at: 1 561 1440 1907 1930 2265 2511 2817 2829
Size: 560 879 467 23 335 246 306 12
Cuts at: 2829 2915 3967 1
Size: 86 1052 449
Fragments arranged by size:
1052 879 560 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 332 332
Size: 4415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 308 353 1252 1658 308
Size: 45 899 406 3065
Fragments arranged by size:
3065 899 406 45
DraI TTT'AAA
Cuts at: 635 1414 635
Size: 779 3636
DrdI GACnn_nn'nnGTC
Cuts at: 245 2121 3465 245
Size: 1876 1344 1195
Fragments arranged by size:
1876 1344 1195
EaeI y'GGCC_r
Cuts at: 1275 2000 2174 2565 2592 3937 1275
Size: 725 174 391 27 1345 1753
Fragments arranged by size:
1753 1345 725 391 174 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 238 2438 2648 238
Size: 2200 210 2005
Fragments arranged by size:
2200 2005 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 3365 3511 4225 2623
Size: 742 146 714 2813
Fragments arranged by size:
2813 742 714 146
EcoICRI GAG'CTC
Cuts at: 1427 1427
Size: 4415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 262 696 1225 1289 2240 2672 3025 3687
Size: 66 434 529 64 951 432 353 662
Cuts at: 3687 196
Size: 924
Fragments arranged by size:
951 924 662 529 434 432 353 66
64
EcoNI CCTnn'n_nnAGG
Cuts at: 905 905
Size: 4415
EcoO109I rG'GnC_Cy
Cuts at: 1554 1554
Size: 4415
EcoRI G'AATT_C
Cuts at: 214 1419 214
Size: 1205 3210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1402 2434 1402
Size: 1032 3383
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 1849 2097 3327 4098 1849
Size: 248 1230 771 2166
Fragments arranged by size:
2166 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2257 2287 2289 2319 2257
Size: 30 2 30 4353
Fragments arranged by size:
4353 30 30 2
HincII GTy'rAC
Cuts at: 1454 1454
Size: 4415
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MfeI C'AATT_G
Cuts at: 773 773
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 639 3174 3358 639
Size: 2535 184 1696
Fragments arranged by size:
2535 1696 184
MscI TGG'CCA
Cuts at: 1277 2176 1277
Size: 899 3516
MslI CAynn'nnrTG
Cuts at: 2404 2531 2404
Size: 127 4288
MspA1I CmG'CkG
Cuts at: 1526 1840 2200 2984 3229 1526
Size: 314 360 784 245 2712
Fragments arranged by size:
2712 784 360 314 245
NaeI GCC'GGC
Cuts at: 2596 2596
Size: 4415
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NgoMIV G'CCGG_C
Cuts at: 2594 2594
Size: 4415
NruI TCG'CGA
Cuts at: 1054 1054
Size: 4415
NspI r_CATG'y
Cuts at: 514 667 1468 1968 3571 514
Size: 153 801 500 1603 1358
Fragments arranged by size:
1603 1358 801 500 153
PacI TTA_AT'TAA
Cuts at: 1109 1109
Size: 4415
PciI A'CATG_T
Cuts at: 3567 3567
Size: 4415
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 4383
PstI C_TGCA'G
Cuts at: 1462 2147 1462
Size: 685 3730
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 1840 2200 1840
Size: 360 4055
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 1429 1429
Size: 4415
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SexAI A'CCwGG_T
Cuts at: 741 741
Size: 4415
SfcI C'TryA_G
Cuts at: 33 1458 2143 3111 3302 33
Size: 1425 685 968 191 1146
Fragments arranged by size:
1425 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 1570 2485 3199 3461 1570
Size: 915 714 262 2524
Fragments arranged by size:
2524 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
StyI C'CwwG_G
Cuts at: 1071 1212 1549 1903 1071
Size: 141 337 354 3583
Fragments arranged by size:
3583 354 337 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 813 1998 2131 2590 2799 813
Size: 1185 133 459 209 2429
Fragments arranged by size:
2429 1185 459 209 133
TatI w'GTAC_w
Cuts at: 1011 3930 1011
Size: 2919 1496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 1344 1376 3932 3964 287
Size: 32 1025 32 2556 32 738
Fragments arranged by size:
2556 1025 738 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 946 2212 946
Size: 1266 3149
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1910 1910
Size: 4415
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PacI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BfrBI BglI BmgBI BmtI BpmI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NsiI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI