(Circular) (Six-base) MAPSORT of: pF1KB9038.seq Check: 1760 from: 1 to: 4445 pF1KB9038 4445 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 829 829 Size: 4445 AccI GT'mk_AC Cuts at: 1483 1483 Size: 4445 Acc65I G'GTAC_C Cuts at: 1461 1461 Size: 4445 AclI AA'CG_TT Cuts at: 4285 4285 Size: 4445 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 591 772 2270 2702 3055 3717 591 Size: 181 1498 432 353 662 1319 Fragments arranged by size: 1498 1319 662 432 353 181 AflIII A'CryG_T Cuts at: 1797 3597 3878 1797 Size: 1800 281 2364 Fragments arranged by size: 2364 1800 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1280 1280 Size: 4445 AlwNI CAG_nnn'CTG Cuts at: 541 1750 3188 541 Size: 1209 1438 1798 Fragments arranged by size: 1798 1438 1209 ApaLI G'TGCA_C Cuts at: 121 3283 121 Size: 3162 1283 ApoI r'AATT_y Cuts at: 1449 3798 4054 1449 Size: 2349 256 1840 Fragments arranged by size: 2349 1840 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4445 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4445 AvaI C'yCGr_G Cuts at: 1465 1465 Size: 4445 BamHI G'GATC_C Cuts at: 1139 1470 1139 Size: 331 4114 BanI G'GyrC_C Cuts at: 157 208 433 615 1461 1875 2123 2158 157 Size: 51 225 182 846 414 248 35 2444 Fragments arranged by size: 2444 846 414 248 225 182 51 35 BanII G_rGCy'C Cuts at: 653 957 1238 1459 2489 653 Size: 304 281 221 1030 2609 Fragments arranged by size: 2609 1030 304 281 221 BbeI G_GCGC'C Cuts at: 437 1879 2127 437 Size: 1442 248 2755 Fragments arranged by size: 2755 1442 248 BbsI GAAGACnn'nnnn_ Cuts at: 978 1047 978 Size: 69 4376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 135 169 135 Size: 34 4411 BciVI GTATCCnnnnn_n' Cuts at: 2343 3399 4445 2343 Size: 1056 1046 2343 Fragments arranged by size: 2343 1056 1046 BclI T'GATC_A Cuts at: 176 1965 176 Size: 1789 2656 BglI GCCn_nnn'nGGC Cuts at: 500 500 Size: 4445 BglII A'GATC_T Cuts at: 356 1960 3997 356 Size: 1604 2037 804 Fragments arranged by size: 2037 1604 804 BlpI GC'TnA_GC Cuts at: 1557 1557 Size: 4445 Bme1580I G_kGCm'C Cuts at: 125 535 553 2070 2163 3287 125 Size: 410 18 1517 93 1124 1283 Fragments arranged by size: 1517 1283 1124 410 93 18 BmgBI CAC'GTC Cuts at: 892 892 Size: 4445 BmrI ACTGGGnnnn_n' Cuts at: 417 532 1657 1738 2073 4217 417 Size: 115 1125 81 335 2144 645 Fragments arranged by size: 2144 1125 645 335 115 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2812 2812 Size: 4445 Bpu10I CC'TnA_GC Cuts at: 339 339 Size: 4445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1174 1621 2500 3214 3512 1174 Size: 447 879 714 298 2107 Fragments arranged by size: 2107 879 714 447 298 BsaI GGTCTCn'nnnn_ Cuts at: 694 4149 694 Size: 3455 990 BsaAI yAC'GTr Cuts at: 1798 1798 Size: 4445 BsaBI GATnn'nnATC Cuts at: 6 1959 3996 6 Size: 1953 2037 455 Fragments arranged by size: 2037 1953 455 BsaHI Gr'CG_yC Cuts at: 434 1876 2124 2826 4322 434 Size: 1442 248 702 1496 557 Fragments arranged by size: 1496 1442 702 557 248 BsaWI w'CCGG_w Cuts at: 1634 2155 2833 3244 3391 3665 1634 Size: 521 678 411 147 274 2414 Fragments arranged by size: 2414 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1417 1420 1435 1447 1417 Size: 3 15 12 4415 Fragments arranged by size: 4415 15 12 3 BseYI C'CCAG_C Cuts at: 977 1870 2628 3293 3701 977 Size: 893 758 665 408 1721 Fragments arranged by size: 1721 893 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1126 1126 Size: 4445 BsiEI CG_ry'CG Cuts at: 104 1032 1987 2033 3263 104 Size: 928 955 46 1230 1286 Fragments arranged by size: 1286 1230 955 928 46 BsiHKAI G_wGCw'C Cuts at: 125 957 1238 1459 2237 2427 3287 125 Size: 832 281 221 778 190 860 1283 Fragments arranged by size: 1283 860 832 778 281 221 190 BsmBI CGTCTCn'nnnn_ Cuts at: 898 1368 898 Size: 470 3975 Bsp1286I G_dGCh'C Cuts at: 125 535 553 653 957 1238 1459 2070 2163 Size: 410 18 100 304 281 221 611 93 Cuts at: 2163 2237 2427 2489 3287 125 Size: 74 190 62 798 1283 Fragments arranged by size: 1283 798 611 410 304 281 221 190 100 93 74 62 18 BspHI T'CATG_A Cuts at: 596 2877 596 Size: 2281 2164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 239 388 829 972 1495 1709 2011 2392 239 Size: 149 441 143 523 214 302 381 2292 Fragments arranged by size: 2292 523 441 381 302 214 149 143 BsrBI CCG'CTC Cuts at: 50 2737 2791 4275 4440 50 Size: 2687 54 1484 165 55 Fragments arranged by size: 2687 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2357 2357 Size: 4445 BsrFI r'CCGG_y Cuts at: 205 2443 2624 205 Size: 2238 181 2026 Fragments arranged by size: 2238 2026 181 BssSI C'ACGA_G Cuts at: 290 1181 2716 3424 290 Size: 891 1535 708 1311 Fragments arranged by size: 1535 1311 891 708 BstAPI GCAn_nnn'nTGC Cuts at: 1822 1822 Size: 4445 BstBI TT'CG_AA Cuts at: 2806 2806 Size: 4445 BstXI CCAn_nnnn'nTGG Cuts at: 303 303 Size: 4445 BstYI r'GATC_y Cuts at: 1 306 356 569 777 1139 1470 1937 1960 Size: 305 50 213 208 362 331 467 23 Cuts at: 1960 2295 2541 2847 2859 2945 3997 1 Size: 335 246 306 12 86 1052 449 Fragments arranged by size: 1052 467 449 362 335 331 306 305 246 213 208 86 50 23 12 BtgI C'CryG_G Cuts at: 216 323 641 933 1026 1043 1059 216 Size: 107 318 292 93 17 16 3602 Fragments arranged by size: 3602 318 292 107 93 17 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2395 2576 2739 2395 Size: 181 163 4101 Fragments arranged by size: 4101 181 163 BtsI GCAGTG_nn' Cuts at: 1262 1688 1262 Size: 426 4019 DraI TTT'AAA Cuts at: 1444 1444 Size: 4445 DraIII CAC_nnn'GTG Cuts at: 1183 1183 Size: 4445 DrdI GACnn_nn'nnGTC Cuts at: 643 2151 3495 643 Size: 1508 1344 1593 Fragments arranged by size: 1593 1508 1344 EaeI y'GGCC_r Cuts at: 351 837 1029 2030 2204 2595 2622 3967 351 Size: 486 192 1001 174 391 27 1345 829 Fragments arranged by size: 1345 1001 829 486 391 192 174 27 EagI C'GGCC_G Cuts at: 1029 2030 1029 Size: 1001 3444 EarI CTCTTCn'nnn_ Cuts at: 1243 1394 1424 2468 2678 1243 Size: 151 30 1044 210 3010 Fragments arranged by size: 3010 1044 210 151 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1264 2653 3395 3541 4255 1264 Size: 1389 742 146 714 1454 Fragments arranged by size: 1454 1389 742 714 146 EcoICRI GAG'CTC Cuts at: 955 1236 1457 955 Size: 281 221 3943 Fragments arranged by size: 3943 281 221 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 591 772 2270 2702 3055 3717 591 Size: 181 1498 432 353 662 1319 Fragments arranged by size: 1498 1319 662 432 353 181 EcoO109I rG'GnC_Cy Cuts at: 343 723 1584 343 Size: 380 861 3204 Fragments arranged by size: 3204 861 380 EcoRI G'AATT_C Cuts at: 1449 1449 Size: 4445 EcoRV GAT'ATC Cuts at: 735 735 Size: 4445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 2464 Size: 4445 FspI TGC'GCA Cuts at: 2226 2226 Size: 4445 HaeII r_GCGC'y Cuts at: 437 1879 2127 3357 4128 437 Size: 1442 248 1230 771 754 Fragments arranged by size: 1442 1230 771 754 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 232 264 1348 1380 2287 2317 2319 2349 232 Size: 32 1084 32 907 30 2 30 2328 Fragments arranged by size: 2328 1084 907 32 32 30 30 2 HincII GTy'rAC Cuts at: 873 1484 873 Size: 611 3834 KasI G'GCGC_C Cuts at: 433 1875 2123 433 Size: 1442 248 2755 Fragments arranged by size: 2755 1442 248 KpnI G_GTAC'C Cuts at: 1465 1465 Size: 4445 MluI A'CGCG_T Cuts at: 3878 3878 Size: 4445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1045 3204 3388 1045 Size: 2159 184 2102 Fragments arranged by size: 2159 2102 184 MscI TGG'CCA Cuts at: 353 839 2206 353 Size: 486 1367 2592 Fragments arranged by size: 2592 1367 486 MslI CAynn'nnrTG Cuts at: 250 301 1267 2434 2561 250 Size: 51 966 1167 127 2134 Fragments arranged by size: 2134 1167 966 127 51 MspA1I CmG'CkG Cuts at: 237 510 1556 1870 2230 3014 3259 237 Size: 273 1046 314 360 784 245 1423 Fragments arranged by size: 1423 1046 784 360 314 273 245 NaeI GCC'GGC Cuts at: 2626 2626 Size: 4445 NarI GG'CG_CC Cuts at: 434 1876 2124 434 Size: 1442 248 2755 Fragments arranged by size: 2755 1442 248 NcoI C'CATG_G Cuts at: 323 323 Size: 4445 NgoMIV G'CCGG_C Cuts at: 2624 2624 Size: 4445 NspI r_CATG'y Cuts at: 1498 1998 3601 1498 Size: 500 1603 2342 Fragments arranged by size: 2342 1603 500 PciI A'CATG_T Cuts at: 3597 3597 Size: 4445 PflMI CCAn_nnn'nTGG Cuts at: 167 522 681 1183 167 Size: 355 159 502 3429 Fragments arranged by size: 3429 502 355 159 PfoI T'CCnGG_A Cuts at: 455 1229 455 Size: 774 3671 PmeI GTTT'AAAC Cuts at: 1444 1444 Size: 4445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1312 1344 2891 2923 1312 Size: 32 1547 32 2834 Fragments arranged by size: 2834 1547 32 32 PpuMI rG'GwC_Cy Cuts at: 343 343 Size: 4445 PshAI GACnn'nnGTC Cuts at: 1162 1162 Size: 4445 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1108 1140 1108 Size: 32 4413 PstI C_TGCA'G Cuts at: 236 500 1492 2177 236 Size: 264 992 685 2504 Fragments arranged by size: 2504 992 685 264 PvuI CG_AT'CG Cuts at: 104 104 Size: 4445 PvuII CAG'CTG Cuts at: 237 510 1870 2230 237 Size: 273 1360 360 2452 Fragments arranged by size: 2452 1360 360 273 RsrII CG'GwC_CG Cuts at: 2640 2640 Size: 4445 SacI G_AGCT'C Cuts at: 957 1238 1459 957 Size: 281 221 3943 Fragments arranged by size: 3943 281 221 SalI G'TCGA_C Cuts at: 1482 1482 Size: 4445 SapI GCTCTTCn'nnn_ Cuts at: 1243 2468 2678 1243 Size: 1225 210 3010 Fragments arranged by size: 3010 1225 210 SbfI CC_TGCA'GG Cuts at: 500 1492 500 Size: 992 3453 ScaI AGT'ACT Cuts at: 3962 3962 Size: 4445 SfcI C'TryA_G Cuts at: 33 232 496 1488 2173 3141 3332 33 Size: 199 264 992 685 968 191 1146 Fragments arranged by size: 1146 992 968 685 264 199 191 SfoI GGC'GCC Cuts at: 435 1877 2125 435 Size: 1442 248 2755 Fragments arranged by size: 2755 1442 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4445 SmaI CCC'GGG Cuts at: 1467 1467 Size: 4445 SmlI C'TyrA_G Cuts at: 1189 1600 2515 3229 3491 1189 Size: 411 915 714 262 2143 Fragments arranged by size: 2143 915 714 411 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4445 SphI G_CATG'C Cuts at: 1498 1998 1498 Size: 500 3945 StuI AGG'CCT Cuts at: 1285 1285 Size: 4445 StyI C'CwwG_G Cuts at: 323 1579 1933 323 Size: 1256 354 2835 Fragments arranged by size: 2835 1256 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 639 991 2028 2161 2620 2829 639 Size: 352 1037 133 459 209 2255 Fragments arranged by size: 2255 1037 459 352 209 133 TatI w'GTAC_w Cuts at: 254 3960 254 Size: 3706 739 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3962 3994 3962 Size: 32 4413 Tth111I GACn'n_nGTC Cuts at: 1360 2242 1360 Size: 882 3563 XbaI T'CTAG_A Cuts at: 309 1476 309 Size: 1167 3278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1002 1940 1002 Size: 938 3507 XmaI C'CCGG_G Cuts at: 1465 1465 Size: 4445 XmnI GAAnn'nnTTC Cuts at: 604 1285 604 Size: 681 3764 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AgeI AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmtI BpmI BsaXI BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI