Restriction map for
pF1KB9038
(Circular) (Six-base) MAPSORT of: pF1KB9038.seq Check: 1760 from: 1 to: 4445
pF1KB9038 4445 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 829
Size: 4445
AccI GT'mk_AC
Cuts at: 1483 1483
Size: 4445
Acc65I G'GTAC_C
Cuts at: 1461 1461
Size: 4445
AclI AA'CG_TT
Cuts at: 4285 4285
Size: 4445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 772 2270 2702 3055 3717 591
Size: 181 1498 432 353 662 1319
Fragments arranged by size:
1498 1319 662 432 353 181
AflIII A'CryG_T
Cuts at: 1797 3597 3878 1797
Size: 1800 281 2364
Fragments arranged by size:
2364 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1280 1280
Size: 4445
AlwNI CAG_nnn'CTG
Cuts at: 541 1750 3188 541
Size: 1209 1438 1798
Fragments arranged by size:
1798 1438 1209
ApaLI G'TGCA_C
Cuts at: 121 3283 121
Size: 3162 1283
ApoI r'AATT_y
Cuts at: 1449 3798 4054 1449
Size: 2349 256 1840
Fragments arranged by size:
2349 1840 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
AvaI C'yCGr_G
Cuts at: 1465 1465
Size: 4445
BamHI G'GATC_C
Cuts at: 1139 1470 1139
Size: 331 4114
BanI G'GyrC_C
Cuts at: 157 208 433 615 1461 1875 2123 2158 157
Size: 51 225 182 846 414 248 35 2444
Fragments arranged by size:
2444 846 414 248 225 182 51 35
BanII G_rGCy'C
Cuts at: 653 957 1238 1459 2489 653
Size: 304 281 221 1030 2609
Fragments arranged by size:
2609 1030 304 281 221
BbeI G_GCGC'C
Cuts at: 437 1879 2127 437
Size: 1442 248 2755
Fragments arranged by size:
2755 1442 248
BbsI GAAGACnn'nnnn_
Cuts at: 978 1047 978
Size: 69 4376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 169 135
Size: 34 4411
BciVI GTATCCnnnnn_n'
Cuts at: 2343 3399 4445 2343
Size: 1056 1046 2343
Fragments arranged by size:
2343 1056 1046
BclI T'GATC_A
Cuts at: 176 1965 176
Size: 1789 2656
BglI GCCn_nnn'nGGC
Cuts at: 500 500
Size: 4445
BglII A'GATC_T
Cuts at: 356 1960 3997 356
Size: 1604 2037 804
Fragments arranged by size:
2037 1604 804
BlpI GC'TnA_GC
Cuts at: 1557 1557
Size: 4445
Bme1580I G_kGCm'C
Cuts at: 125 535 553 2070 2163 3287 125
Size: 410 18 1517 93 1124 1283
Fragments arranged by size:
1517 1283 1124 410 93 18
BmgBI CAC'GTC
Cuts at: 892 892
Size: 4445
BmrI ACTGGGnnnn_n'
Cuts at: 417 532 1657 1738 2073 4217 417
Size: 115 1125 81 335 2144 645
Fragments arranged by size:
2144 1125 645 335 115 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 4445
Bpu10I CC'TnA_GC
Cuts at: 339 339
Size: 4445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 1621 2500 3214 3512 1174
Size: 447 879 714 298 2107
Fragments arranged by size:
2107 879 714 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 694 4149 694
Size: 3455 990
BsaAI yAC'GTr
Cuts at: 1798 1798
Size: 4445
BsaBI GATnn'nnATC
Cuts at: 6 1959 3996 6
Size: 1953 2037 455
Fragments arranged by size:
2037 1953 455
BsaHI Gr'CG_yC
Cuts at: 434 1876 2124 2826 4322 434
Size: 1442 248 702 1496 557
Fragments arranged by size:
1496 1442 702 557 248
BsaWI w'CCGG_w
Cuts at: 1634 2155 2833 3244 3391 3665 1634
Size: 521 678 411 147 274 2414
Fragments arranged by size:
2414 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1417 1420 1435 1447 1417
Size: 3 15 12 4415
Fragments arranged by size:
4415 15 12 3
BseYI C'CCAG_C
Cuts at: 977 1870 2628 3293 3701 977
Size: 893 758 665 408 1721
Fragments arranged by size:
1721 893 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1126
Size: 4445
BsiEI CG_ry'CG
Cuts at: 104 1032 1987 2033 3263 104
Size: 928 955 46 1230 1286
Fragments arranged by size:
1286 1230 955 928 46
BsiHKAI G_wGCw'C
Cuts at: 125 957 1238 1459 2237 2427 3287 125
Size: 832 281 221 778 190 860 1283
Fragments arranged by size:
1283 860 832 778 281 221 190
BsmBI CGTCTCn'nnnn_
Cuts at: 898 1368 898
Size: 470 3975
Bsp1286I G_dGCh'C
Cuts at: 125 535 553 653 957 1238 1459 2070 2163
Size: 410 18 100 304 281 221 611 93
Cuts at: 2163 2237 2427 2489 3287 125
Size: 74 190 62 798 1283
Fragments arranged by size:
1283 798 611 410 304 281 221 190
100 93 74 62 18
BspHI T'CATG_A
Cuts at: 596 2877 596
Size: 2281 2164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 388 829 972 1495 1709 2011 2392 239
Size: 149 441 143 523 214 302 381 2292
Fragments arranged by size:
2292 523 441 381 302 214 149 143
BsrBI CCG'CTC
Cuts at: 50 2737 2791 4275 4440 50
Size: 2687 54 1484 165 55
Fragments arranged by size:
2687 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2357 2357
Size: 4445
BsrFI r'CCGG_y
Cuts at: 205 2443 2624 205
Size: 2238 181 2026
Fragments arranged by size:
2238 2026 181
BssSI C'ACGA_G
Cuts at: 290 1181 2716 3424 290
Size: 891 1535 708 1311
Fragments arranged by size:
1535 1311 891 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1822 1822
Size: 4445
BstBI TT'CG_AA
Cuts at: 2806 2806
Size: 4445
BstXI CCAn_nnnn'nTGG
Cuts at: 303 303
Size: 4445
BstYI r'GATC_y
Cuts at: 1 306 356 569 777 1139 1470 1937 1960
Size: 305 50 213 208 362 331 467 23
Cuts at: 1960 2295 2541 2847 2859 2945 3997 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 362 335 331 306 305
246 213 208 86 50 23 12
BtgI C'CryG_G
Cuts at: 216 323 641 933 1026 1043 1059 216
Size: 107 318 292 93 17 16 3602
Fragments arranged by size:
3602 318 292 107 93 17 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2395 2576 2739 2395
Size: 181 163 4101
Fragments arranged by size:
4101 181 163
BtsI GCAGTG_nn'
Cuts at: 1262 1688 1262
Size: 426 4019
DraI TTT'AAA
Cuts at: 1444 1444
Size: 4445
DraIII CAC_nnn'GTG
Cuts at: 1183 1183
Size: 4445
DrdI GACnn_nn'nnGTC
Cuts at: 643 2151 3495 643
Size: 1508 1344 1593
Fragments arranged by size:
1593 1508 1344
EaeI y'GGCC_r
Cuts at: 351 837 1029 2030 2204 2595 2622 3967 351
Size: 486 192 1001 174 391 27 1345 829
Fragments arranged by size:
1345 1001 829 486 391 192 174 27
EagI C'GGCC_G
Cuts at: 1029 2030 1029
Size: 1001 3444
EarI CTCTTCn'nnn_
Cuts at: 1243 1394 1424 2468 2678 1243
Size: 151 30 1044 210 3010
Fragments arranged by size:
3010 1044 210 151 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1264 2653 3395 3541 4255 1264
Size: 1389 742 146 714 1454
Fragments arranged by size:
1454 1389 742 714 146
EcoICRI GAG'CTC
Cuts at: 955 1236 1457 955
Size: 281 221 3943
Fragments arranged by size:
3943 281 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 772 2270 2702 3055 3717 591
Size: 181 1498 432 353 662 1319
Fragments arranged by size:
1498 1319 662 432 353 181
EcoO109I rG'GnC_Cy
Cuts at: 343 723 1584 343
Size: 380 861 3204
Fragments arranged by size:
3204 861 380
EcoRI G'AATT_C
Cuts at: 1449 1449
Size: 4445
EcoRV GAT'ATC
Cuts at: 735 735
Size: 4445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4445
FspI TGC'GCA
Cuts at: 2226 2226
Size: 4445
HaeII r_GCGC'y
Cuts at: 437 1879 2127 3357 4128 437
Size: 1442 248 1230 771 754
Fragments arranged by size:
1442 1230 771 754 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 1348 1380 2287 2317 2319 2349 232
Size: 32 1084 32 907 30 2 30 2328
Fragments arranged by size:
2328 1084 907 32 32 30 30 2
HincII GTy'rAC
Cuts at: 873 1484 873
Size: 611 3834
KasI G'GCGC_C
Cuts at: 433 1875 2123 433
Size: 1442 248 2755
Fragments arranged by size:
2755 1442 248
KpnI G_GTAC'C
Cuts at: 1465 1465
Size: 4445
MluI A'CGCG_T
Cuts at: 3878 3878
Size: 4445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1045 3204 3388 1045
Size: 2159 184 2102
Fragments arranged by size:
2159 2102 184
MscI TGG'CCA
Cuts at: 353 839 2206 353
Size: 486 1367 2592
Fragments arranged by size:
2592 1367 486
MslI CAynn'nnrTG
Cuts at: 250 301 1267 2434 2561 250
Size: 51 966 1167 127 2134
Fragments arranged by size:
2134 1167 966 127 51
MspA1I CmG'CkG
Cuts at: 237 510 1556 1870 2230 3014 3259 237
Size: 273 1046 314 360 784 245 1423
Fragments arranged by size:
1423 1046 784 360 314 273 245
NaeI GCC'GGC
Cuts at: 2626 2626
Size: 4445
NarI GG'CG_CC
Cuts at: 434 1876 2124 434
Size: 1442 248 2755
Fragments arranged by size:
2755 1442 248
NcoI C'CATG_G
Cuts at: 323 323
Size: 4445
NgoMIV G'CCGG_C
Cuts at: 2624 2624
Size: 4445
NspI r_CATG'y
Cuts at: 1498 1998 3601 1498
Size: 500 1603 2342
Fragments arranged by size:
2342 1603 500
PciI A'CATG_T
Cuts at: 3597 3597
Size: 4445
PflMI CCAn_nnn'nTGG
Cuts at: 167 522 681 1183 167
Size: 355 159 502 3429
Fragments arranged by size:
3429 502 355 159
PfoI T'CCnGG_A
Cuts at: 455 1229 455
Size: 774 3671
PmeI GTTT'AAAC
Cuts at: 1444 1444
Size: 4445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1312 1344 2891 2923 1312
Size: 32 1547 32 2834
Fragments arranged by size:
2834 1547 32 32
PpuMI rG'GwC_Cy
Cuts at: 343 343
Size: 4445
PshAI GACnn'nnGTC
Cuts at: 1162 1162
Size: 4445
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1108 1140 1108
Size: 32 4413
PstI C_TGCA'G
Cuts at: 236 500 1492 2177 236
Size: 264 992 685 2504
Fragments arranged by size:
2504 992 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4445
PvuII CAG'CTG
Cuts at: 237 510 1870 2230 237
Size: 273 1360 360 2452
Fragments arranged by size:
2452 1360 360 273
RsrII CG'GwC_CG
Cuts at: 2640 2640
Size: 4445
SacI G_AGCT'C
Cuts at: 957 1238 1459 957
Size: 281 221 3943
Fragments arranged by size:
3943 281 221
SalI G'TCGA_C
Cuts at: 1482 1482
Size: 4445
SapI GCTCTTCn'nnn_
Cuts at: 1243 2468 2678 1243
Size: 1225 210 3010
Fragments arranged by size:
3010 1225 210
SbfI CC_TGCA'GG
Cuts at: 500 1492 500
Size: 992 3453
ScaI AGT'ACT
Cuts at: 3962 3962
Size: 4445
SfcI C'TryA_G
Cuts at: 33 232 496 1488 2173 3141 3332 33
Size: 199 264 992 685 968 191 1146
Fragments arranged by size:
1146 992 968 685 264 199 191
SfoI GGC'GCC
Cuts at: 435 1877 2125 435
Size: 1442 248 2755
Fragments arranged by size:
2755 1442 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
SmaI CCC'GGG
Cuts at: 1467 1467
Size: 4445
SmlI C'TyrA_G
Cuts at: 1189 1600 2515 3229 3491 1189
Size: 411 915 714 262 2143
Fragments arranged by size:
2143 915 714 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4445
SphI G_CATG'C
Cuts at: 1498 1998 1498
Size: 500 3945
StuI AGG'CCT
Cuts at: 1285 1285
Size: 4445
StyI C'CwwG_G
Cuts at: 323 1579 1933 323
Size: 1256 354 2835
Fragments arranged by size:
2835 1256 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 639 991 2028 2161 2620 2829 639
Size: 352 1037 133 459 209 2255
Fragments arranged by size:
2255 1037 459 352 209 133
TatI w'GTAC_w
Cuts at: 254 3960 254
Size: 3706 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4413
Tth111I GACn'n_nGTC
Cuts at: 1360 2242 1360
Size: 882 3563
XbaI T'CTAG_A
Cuts at: 309 1476 309
Size: 1167 3278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1002 1940 1002
Size: 938 3507
XmaI C'CCGG_G
Cuts at: 1465 1465
Size: 4445
XmnI GAAnn'nnTTC
Cuts at: 604 1285 604
Size: 681 3764
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmtI BpmI BsaXI
BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI