Restriction map for
pF1KB9055
(Circular) (Six-base) MAPSORT of: pF1KB9055.seq Check: 9219 from: 1 to: 6047
pF1KB9055 6047 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 3085 3085
Size: 6047
Acc65I G'GTAC_C
Cuts at: 507 3063 507
Size: 2556 3491
AclI AA'CG_TT
Cuts at: 2180 5887 2180
Size: 3707 2340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 932 989 1277 1388 1523 1715 3872 4304
Size: 63 57 288 111 135 192 2157 432
Cuts at: 4304 4657 5319 869
Size: 353 662 1597
Fragments arranged by size:
2157 1597 662 432 353 288 192 135
111 63 57
AflIII A'CryG_T
Cuts at: 266 939 1372 3399 5199 5480 266
Size: 673 433 2027 1800 281 833
Fragments arranged by size:
2027 1800 833 673 433 281
AlwNI CAG_nnn'CTG
Cuts at: 718 3352 4790 718
Size: 2634 1438 1975
Fragments arranged by size:
2634 1975 1438
ApaLI G'TGCA_C
Cuts at: 4885 4885
Size: 6047
ApoI r'AATT_y
Cuts at: 1262 1292 1365 1477 1528 1651 1792 1922 2532
Size: 30 73 112 51 123 141 130 610
Cuts at: 2532 2683 2857 3051 5400 5656 1262
Size: 151 174 194 2349 256 1653
Fragments arranged by size:
2349 1653 610 256 194 174 151 141
130 123 112 73 51 30
AseI AT'TA_AT
Cuts at: 20 2894 20
Size: 2874 3173
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6047
AvaI C'yCGr_G
Cuts at: 196 592 2778 3067 196
Size: 396 2186 289 3176
Fragments arranged by size:
3176 2186 396 289
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 491 524 491
Size: 33 6014
BamHI G'GATC_C
Cuts at: 3072 3072
Size: 6047
BanI G'GyrC_C
Cuts at: 507 2316 3063 3477 3725 3760 507
Size: 1809 747 414 248 35 2794
Fragments arranged by size:
2794 1809 747 414 248 35
BanII G_rGCy'C
Cuts at: 2518 3061 4091 2518
Size: 543 1030 4474
Fragments arranged by size:
4474 1030 543
BbeI G_GCGC'C
Cuts at: 3481 3729 3481
Size: 248 5799
BbsI GAAGACnn'nnnn_
Cuts at: 2354 2397 2579 2354
Size: 43 182 5822
Fragments arranged by size:
5822 182 43
BbvCI CC'TCA_GC
Cuts at: 2142 2142
Size: 6047
BciVI GTATCCnnnnn_n'
Cuts at: 3945 5001 6047 3945
Size: 1056 1046 3945
Fragments arranged by size:
3945 1056 1046
BclI T'GATC_A
Cuts at: 2910 3567 2910
Size: 657 5390
BfrBI ATG'CAT
Cuts at: 378 378
Size: 6047
BglI GCCn_nnn'nGGC
Cuts at: 238 238
Size: 6047
BglII A'GATC_T
Cuts at: 1233 3562 5599 1233
Size: 2329 2037 1681
Fragments arranged by size:
2329 2037 1681
BlpI GC'TnA_GC
Cuts at: 3159 3159
Size: 6047
Bme1580I G_kGCm'C
Cuts at: 3672 3765 4889 3672
Size: 93 1124 4830
Fragments arranged by size:
4830 1124 93
BmgBI CAC'GTC
Cuts at: 267 295 267
Size: 28 6019
BmrI ACTGGGnnnn_n'
Cuts at: 3259 3340 3675 5819 3259
Size: 81 335 2144 3487
Fragments arranged by size:
3487 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 171 2520 4414 171
Size: 2349 1894 1804
Fragments arranged by size:
2349 1894 1804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 1324 2440 722
Size: 602 1116 4329
Fragments arranged by size:
4329 1116 602
Bpu10I CC'TnA_GC
Cuts at: 2142 2142
Size: 6047
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1784 3223 4102 4816 5114 1154
Size: 630 1439 879 714 298 2087
Fragments arranged by size:
2087 1439 879 714 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 530 657 1011 5751 530
Size: 127 354 4740 826
Fragments arranged by size:
4740 826 354 127
BsaAI yAC'GTr
Cuts at: 1132 1375 1557 3038 3400 1132
Size: 243 182 1481 362 3779
Fragments arranged by size:
3779 1481 362 243 182
BsaBI GATnn'nnATC
Cuts at: 6 851 3561 5598 6
Size: 845 2710 2037 455
Fragments arranged by size:
2710 2037 845 455
BsaHI Gr'CG_yC
Cuts at: 3478 3726 4428 5924 3478
Size: 248 702 1496 3601
Fragments arranged by size:
3601 1496 702 248
BsaWI w'CCGG_w
Cuts at: 567 1191 3236 3757 4435 4846 4993 5267 567
Size: 624 2045 521 678 411 147 274 1347
Fragments arranged by size:
2045 1347 678 624 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1852 1882 1852
Size: 30 6017
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 2496 163
Size: 2333 3714
BseYI C'CCAG_C
Cuts at: 1069 2800 3472 4230 4895 5303 1069
Size: 1731 672 758 665 408 1813
Fragments arranged by size:
1813 1731 758 672 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 577
Size: 6047
BsiEI CG_ry'CG
Cuts at: 104 3589 3635 4865 104
Size: 3485 46 1230 1286
Fragments arranged by size:
3485 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1501 2503 3061 3839 4029 4889 1501
Size: 1002 558 778 190 860 2659
Fragments arranged by size:
2659 1002 860 778 558 190
BsmI GAATG_Cn'
Cuts at: 1116 2232 2408 1116
Size: 1116 176 4755
Fragments arranged by size:
4755 1116 176
BsmBI CGTCTCn'nnnn_
Cuts at: 2476 2476
Size: 6047
Bsp1286I G_dGCh'C
Cuts at: 1501 2503 2518 3061 3672 3765 3839 4029 4091
Size: 1002 15 543 611 93 74 190 62
Cuts at: 4091 4889 1501
Size: 798 2659
Fragments arranged by size:
2659 1002 798 611 543 190 93 74
62 15
BspEI T'CCGG_A
Cuts at: 567 567
Size: 6047
BspHI T'CATG_A
Cuts at: 4479 4479
Size: 6047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3097 3311 3613 3994 3097
Size: 214 302 381 5150
Fragments arranged by size:
5150 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4339 4393 5877 6042 50
Size: 4289 54 1484 165 55
Fragments arranged by size:
4289 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 128 245 900 3959 128
Size: 117 655 3059 2216
Fragments arranged by size:
3059 2216 655 117
BsrFI r'CCGG_y
Cuts at: 4045 4226 4045
Size: 181 5866
BssSI C'ACGA_G
Cuts at: 2195 4318 5026 2195
Size: 2123 708 3216
Fragments arranged by size:
3216 2123 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3424 3424
Size: 6047
BstBI TT'CG_AA
Cuts at: 2151 4408 2151
Size: 2257 3790
BstEII G'GTnAC_C
Cuts at: 2103 2103
Size: 6047
BstXI CCAn_nnnn'nTGG
Cuts at: 2808 2808
Size: 6047
BstYI r'GATC_y
Cuts at: 1 840 1233 2831 3072 3539 3562 3897 4143
Size: 839 393 1598 241 467 23 335 246
Cuts at: 4143 4449 4461 4547 5599 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1598 1052 839 467 449 393 335 306
246 241 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2372 2372
Size: 6047
BtgI C'CryG_G
Cuts at: 107 1947 2108 107
Size: 1840 161 4046
Fragments arranged by size:
4046 1840 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3997 4178 4341 3997
Size: 181 163 5703
Fragments arranged by size:
5703 181 163
BtsI GCAGTG_nn'
Cuts at: 257 3290 257
Size: 3033 3014
DraI TTT'AAA
Cuts at: 585 3046 585
Size: 2461 3586
DrdI GACnn_nn'nnGTC
Cuts at: 271 2066 3753 5097 271
Size: 1795 1687 1344 1221
Fragments arranged by size:
1795 1687 1344 1221
EaeI y'GGCC_r
Cuts at: 218 3632 3806 4197 4224 5569 218
Size: 3414 174 391 27 1345 696
Fragments arranged by size:
3414 1345 696 391 174 27
EagI C'GGCC_G
Cuts at: 3632 3632
Size: 6047
EarI CTCTTCn'nnn_
Cuts at: 1769 2272 4070 4280 1769
Size: 503 1798 210 3536
Fragments arranged by size:
3536 1798 503 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4255 4997 5143 5857 4255
Size: 742 146 714 4445
Fragments arranged by size:
4445 742 714 146
EcoICRI GAG'CTC
Cuts at: 3059 3059
Size: 6047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 869 932 989 1277 1324 1388 1523 1715
Size: 147 63 57 288 47 64 135 192
Cuts at: 1715 2440 3872 4304 4657 5319 722
Size: 725 1432 432 353 662 1450
Fragments arranged by size:
1450 1432 725 662 432 353 288 192
147 135 64 63 57 47
EcoO109I rG'GnC_Cy
Cuts at: 963 2116 2929 3186 963
Size: 1153 813 257 3824
Fragments arranged by size:
3824 1153 813 257
EcoRI G'AATT_C
Cuts at: 1262 1292 1365 1922 3051 1262
Size: 30 73 557 1129 4258
Fragments arranged by size:
4258 1129 557 73 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4066 4066
Size: 6047
FspI TGC'GCA
Cuts at: 3828 3828
Size: 6047
HaeII r_GCGC'y
Cuts at: 3481 3729 4959 5730 3481
Size: 248 1230 771 3798
Fragments arranged by size:
3798 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 1443 1475 2992 3024 3889 3919 3921
Size: 32 1275 32 1517 32 865 30 2
Cuts at: 3921 3951 136
Size: 30 2232
Fragments arranged by size:
2232 1517 1275 865 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3086 3086
Size: 6047
HindIII A'AGCT_T
Cuts at: 485 2039 485
Size: 1554 4493
KasI G'GCGC_C
Cuts at: 3477 3725 3477
Size: 248 5799
KpnI G_GTAC'C
Cuts at: 511 3067 511
Size: 2556 3491
MluI A'CGCG_T
Cuts at: 5480 5480
Size: 6047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 258 2659 2945 4806 4990 258
Size: 2401 286 1861 184 1315
Fragments arranged by size:
2401 1861 1315 286 184
MscI TGG'CCA
Cuts at: 220 3808 220
Size: 3588 2459
MslI CAynn'nnrTG
Cuts at: 250 692 763 907 1544 1633 2590 4036 4163
Size: 442 71 144 637 89 957 1446 127
Cuts at: 4163 250
Size: 2134
Fragments arranged by size:
2134 1446 957 637 442 144 127 89
71
MspA1I CmG'CkG
Cuts at: 814 1949 2146 2904 2950 3158 3472 3832 4616
Size: 1135 197 758 46 208 314 360 784
Cuts at: 4616 4861 814
Size: 245 2000
Fragments arranged by size:
2000 1135 784 758 360 314 245 208
197 46
NaeI GCC'GGC
Cuts at: 4228 4228
Size: 6047
NarI GG'CG_CC
Cuts at: 3478 3726 3478
Size: 248 5799
NcoI C'CATG_G
Cuts at: 107 107
Size: 6047
NdeI CA'TA_TG
Cuts at: 519 519
Size: 6047
NgoMIV G'CCGG_C
Cuts at: 4226 4226
Size: 6047
NsiI A_TGCA'T
Cuts at: 380 380
Size: 6047
NspI r_CATG'y
Cuts at: 691 943 2775 3100 3600 5203 691
Size: 252 1832 325 500 1603 1535
Fragments arranged by size:
1832 1603 1535 500 325 252
PciI A'CATG_T
Cuts at: 939 5199 939
Size: 4260 1787
PfoI T'CCnGG_A
Cuts at: 1052 2342 2880 1052
Size: 1290 538 4219
Fragments arranged by size:
4219 1290 538
PmeI GTTT'AAAC
Cuts at: 3046 3046
Size: 6047
PmlI CAC'GTG
Cuts at: 1375 3038 1375
Size: 1663 4384
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 6015
PpuMI rG'GwC_Cy
Cuts at: 963 963
Size: 6047
PstI C_TGCA'G
Cuts at: 372 3094 3779 372
Size: 2722 685 2640
Fragments arranged by size:
2722 2640 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6047
PvuII CAG'CTG
Cuts at: 814 2146 2904 2950 3472 3832 814
Size: 1332 758 46 522 360 3029
Fragments arranged by size:
3029 1332 758 522 360 46
RsrII CG'GwC_CG
Cuts at: 4242 4242
Size: 6047
SacI G_AGCT'C
Cuts at: 3061 3061
Size: 6047
SacII CC_GC'GG
Cuts at: 1950 1950
Size: 6047
SalI G'TCGA_C
Cuts at: 3084 3084
Size: 6047
SapI GCTCTTCn'nnn_
Cuts at: 4070 4280 4070
Size: 210 5837
SbfI CC_TGCA'GG
Cuts at: 3094 3094
Size: 6047
ScaI AGT'ACT
Cuts at: 5564 5564
Size: 6047
SexAI A'CCwGG_T
Cuts at: 1912 1912
Size: 6047
SfcI C'TryA_G
Cuts at: 33 368 425 2727 3090 3775 4743 4934 33
Size: 335 57 2302 363 685 968 191 1146
Fragments arranged by size:
2302 1146 968 685 363 335 191 57
SfoI GGC'GCC
Cuts at: 3479 3727 3479
Size: 248 5799
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6047
SmaI CCC'GGG
Cuts at: 2780 3069 2780
Size: 289 5758
SmlI C'TyrA_G
Cuts at: 196 1169 1799 3202 4117 4831 5093 196
Size: 973 630 1403 915 714 262 1150
Fragments arranged by size:
1403 1150 973 915 714 630 262
SnaBI TAC'GTA
Cuts at: 1132 1132
Size: 6047
SpeI A'CTAG_T
Cuts at: 67 1989 67
Size: 1922 4125
SphI G_CATG'C
Cuts at: 2775 3100 3600 2775
Size: 325 500 5222
Fragments arranged by size:
5222 500 325
SspI AAT'ATT
Cuts at: 343 343
Size: 6047
StuI AGG'CCT
Cuts at: 2221 2221
Size: 6047
StyI C'CwwG_G
Cuts at: 107 1490 1508 1612 3181 3535 107
Size: 1383 18 104 1569 354 2619
Fragments arranged by size:
2619 1569 1383 354 104 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2556 3630 3763 4222 4431 2556
Size: 1074 133 459 209 4172
Fragments arranged by size:
4172 1074 459 209 133
TatI w'GTAC_w
Cuts at: 5562 5562
Size: 6047
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2673 2705 5564 5596 2673
Size: 32 2859 32 3124
Fragments arranged by size:
3124 2859 32 32
Tth111I GACn'n_nGTC
Cuts at: 3844 3844
Size: 6047
XbaI T'CTAG_A
Cuts at: 3078 3078
Size: 6047
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 3035 3542 234
Size: 2801 507 2739
Fragments arranged by size:
2801 2739 507
XhoI C'TCGA_G
Cuts at: 196 196
Size: 6047
XmaI C'CCGG_G
Cuts at: 2778 3067 2778
Size: 289 5758
XmnI GAAnn'nnTTC
Cuts at: 1250 1262 1331 1577 1250
Size: 12 69 246 5720
Fragments arranged by size:
5720 246 69 12
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BcgI BmtI BsiWI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PflMI PshAI PsiI PspOMI PspXI PsrI SanDI SfiI
SgrAI SrfI SwaI ZraI