(Circular) (Six-base) MAPSORT of: pF1KB9097.seq Check: 3440 from: 1 to: 7211 pF1KB9097 7211 bp With 182 enzymes: SgfI * September 18, 2009 17:00 .. AatII G_ACGT'C Cuts at: 1583 3689 1583 Size: 2106 5105 AccI GT'mk_AC Cuts at: 4249 4249 Size: 7211 Acc65I G'GTAC_C Cuts at: 1640 4227 1640 Size: 2587 4624 AclI AA'CG_TT Cuts at: 7051 7051 Size: 7211 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 265 1205 1840 1916 2243 3030 5036 5468 6215 Size: 940 635 76 327 787 2006 432 747 Cuts at: 6215 6483 265 Size: 268 993 Fragments arranged by size: 2006 993 940 787 747 635 432 327 268 76 AfeI AGC'GCT Cuts at: 3051 3051 Size: 7211 AflIII A'CryG_T Cuts at: 760 4563 5667 6644 760 Size: 3803 1104 977 1327 Fragments arranged by size: 3803 1327 1104 977 AgeI A'CCGG_T Cuts at: 1637 5599 1637 Size: 3962 3249 AhdI GACnn_n'nnGTC Cuts at: 3690 3690 Size: 7211 AleI CACnn'nnGTG Cuts at: 1870 1870 Size: 7211 AlwNI CAG_nnn'CTG Cuts at: 243 1398 2863 4516 6083 243 Size: 1155 1465 1653 1567 1371 Fragments arranged by size: 1653 1567 1465 1371 1155 ApaLI G'TGCA_C Cuts at: 777 5981 777 Size: 5204 2007 ApoI r'AATT_y Cuts at: 1481 2277 4215 6564 6820 1481 Size: 796 1938 2349 256 1872 Fragments arranged by size: 2349 1938 1872 796 256 AseI AT'TA_AT Cuts at: 20 20 Size: 7211 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7211 AvaI C'yCGr_G Cuts at: 385 518 805 1032 2799 3089 3967 4231 385 Size: 133 287 227 1767 290 878 264 3365 Fragments arranged by size: 3365 1767 878 290 287 264 227 133 AvrII C'CTAG_G Cuts at: 1332 1332 Size: 7211 BamHI G'GATC_C Cuts at: 4236 4236 Size: 7211 BanI G'GyrC_C Cuts at: 1517 1634 1640 2134 4227 4641 4889 4924 1517 Size: 117 6 494 2093 414 248 35 3804 Fragments arranged by size: 3804 2093 494 414 248 117 35 6 BanII G_rGCy'C Cuts at: 155 1247 1799 4225 5255 155 Size: 1092 552 2426 1030 2111 Fragments arranged by size: 2426 2111 1092 1030 552 BbeI G_GCGC'C Cuts at: 1521 4645 4893 1521 Size: 3124 248 3839 Fragments arranged by size: 3839 3124 248 BbsI GAAGACnn'nnnn_ Cuts at: 694 2967 3660 4187 694 Size: 2273 693 527 3718 Fragments arranged by size: 3718 2273 693 527 BbvCI CC'TCA_GC Cuts at: 964 964 Size: 7211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 252 286 3751 3785 252 Size: 34 3465 34 3678 Fragments arranged by size: 3678 3465 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5109 5870 7211 5109 Size: 761 1341 5109 Fragments arranged by size: 5109 1341 761 BclI T'GATC_A Cuts at: 2912 4731 2912 Size: 1819 5392 BglI GCCn_nnn'nGGC Cuts at: 1034 3727 1034 Size: 2693 4518 BglII A'GATC_T Cuts at: 1907 4726 6763 1907 Size: 2819 2037 2355 Fragments arranged by size: 2819 2355 2037 BlpI GC'TnA_GC Cuts at: 4323 4323 Size: 7211 Bme1580I G_kGCm'C Cuts at: 754 781 1030 2306 4836 4929 5985 754 Size: 27 249 1276 2530 93 1056 1980 Fragments arranged by size: 2530 1980 1276 1056 249 93 27 BmgBI CAC'GTC Cuts at: 1607 1767 1791 1607 Size: 160 24 7027 Fragments arranged by size: 7027 160 24 BmrI ACTGGGnnnn_n' Cuts at: 1416 4185 4423 4504 4839 6983 1416 Size: 2769 238 81 335 2144 1644 Fragments arranged by size: 2769 2144 1644 335 238 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1047 1581 5578 1047 Size: 534 3997 2680 Fragments arranged by size: 3997 2680 534 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 274 1249 2440 3904 4009 274 Size: 975 1191 1464 105 3476 Fragments arranged by size: 3476 1464 1191 975 105 Bpu10I CC'TnA_GC Cuts at: 964 3430 964 Size: 2466 4745 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 466 3640 4387 5266 5758 6056 466 Size: 3174 747 879 492 298 1621 Fragments arranged by size: 3174 1621 879 747 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 392 1302 3386 3500 3939 6915 392 Size: 910 2084 114 439 2976 688 Fragments arranged by size: 2976 2084 910 688 439 114 BsaAI yAC'GTr Cuts at: 761 1269 4564 761 Size: 508 3295 3408 Fragments arranged by size: 3408 3295 508 BsaBI GATnn'nnATC Cuts at: 6 4725 6762 6 Size: 4719 2037 455 Fragments arranged by size: 4719 2037 455 BsaHI Gr'CG_yC Cuts at: 1518 1580 3686 4642 4890 5592 7088 1518 Size: 62 2106 956 248 702 1496 1641 Fragments arranged by size: 2106 1641 1496 956 702 248 62 BsaWI w'CCGG_w Cuts at: 121 1637 3377 3602 4400 4921 5599 5873 6020 Size: 1516 1740 225 798 521 678 274 147 Cuts at: 6020 6431 121 Size: 411 901 Fragments arranged by size: 1740 1516 901 798 678 521 411 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2413 2443 2942 2972 2413 Size: 30 499 30 6652 Fragments arranged by size: 6652 499 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 140 682 1354 1965 2164 2263 3922 3967 3991 Size: 542 672 611 199 99 1659 45 24 Cuts at: 3991 4023 140 Size: 32 3328 Fragments arranged by size: 3328 1659 672 611 542 199 99 45 32 24 BseYI C'CCAG_C Cuts at: 2544 3003 3300 4636 5394 5971 6467 2544 Size: 459 297 1336 758 577 496 3288 Fragments arranged by size: 3288 1336 758 577 496 459 297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 246 2269 246 Size: 2023 5188 BsiEI CG_ry'CG Cuts at: 104 2384 4753 4799 6007 104 Size: 2280 2369 46 1208 1308 Fragments arranged by size: 2369 2280 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 781 4225 5003 5193 5985 781 Size: 3444 778 190 792 2007 Fragments arranged by size: 3444 2007 792 778 190 Bsp1286I G_dGCh'C Cuts at: 155 754 781 1030 1247 1799 2306 4225 4836 Size: 599 27 249 217 552 507 1919 611 Cuts at: 4836 4929 5003 5193 5255 5985 155 Size: 93 74 190 62 730 1381 Fragments arranged by size: 1919 1381 730 611 599 552 507 249 217 190 93 74 62 27 BspEI T'CCGG_A Cuts at: 121 6431 121 Size: 6310 901 BspHI T'CATG_A Cuts at: 6387 6387 Size: 7211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 4210 4261 4475 4777 5158 4210 Size: 51 214 302 381 6263 Fragments arranged by size: 6263 381 302 214 51 BsrBI CCG'CTC Cuts at: 50 4173 5503 5557 7041 7206 50 Size: 4123 1330 54 1484 165 55 Fragments arranged by size: 4123 1484 1330 165 55 54 BsrDI GCAATG_nn' Cuts at: 1214 5123 1214 Size: 3909 3302 BsrFI r'CCGG_y Cuts at: 877 1637 1643 2081 2789 5209 5390 5599 877 Size: 760 6 438 708 2420 181 209 2489 Fragments arranged by size: 2489 2420 760 708 438 209 181 6 BsrGI T'GTAC_A Cuts at: 1913 1913 Size: 7211 BssHII G'CGCG_C Cuts at: 827 827 Size: 7211 BssSI C'ACGA_G Cuts at: 2530 5482 5840 2530 Size: 2952 358 3901 Fragments arranged by size: 3901 2952 358 BstAPI GCAn_nnn'nTGC Cuts at: 1067 4588 1067 Size: 3521 3690 BstBI TT'CG_AA Cuts at: 5572 5572 Size: 7211 BstXI CCAn_nnnn'nTGG Cuts at: 1891 1950 2624 3165 1891 Size: 59 674 541 5937 Fragments arranged by size: 5937 674 541 59 BstYI r'GATC_y Cuts at: 1 1274 1662 1907 2859 4236 4703 4726 5061 Size: 1273 388 245 952 1377 467 23 335 Cuts at: 5061 5307 6319 6405 6417 6763 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1377 1273 1012 952 467 449 388 346 335 246 245 86 23 12 Bsu36I CC'TnA_GG Cuts at: 505 2063 505 Size: 1558 5653 BtgI C'CryG_G Cuts at: 426 662 1868 1890 2378 426 Size: 236 1206 22 488 5259 Fragments arranged by size: 5259 1206 488 236 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2901 5161 5342 5505 2901 Size: 2260 181 163 4607 Fragments arranged by size: 4607 2260 181 163 BtsI GCAGTG_nn' Cuts at: 4048 4454 4048 Size: 406 6805 ClaI AT'CG_AT Cuts at: 2510 2510 Size: 7211 DraI TTT'AAA Cuts at: 2657 4210 2657 Size: 1553 5658 DraIII CAC_nnn'GTG Cuts at: 3288 3288 Size: 7211 DrdI GACnn_nn'nnGTC Cuts at: 4917 5775 4917 Size: 858 6353 EaeI y'GGCC_r Cuts at: 184 2342 2381 2662 3194 3449 3728 4796 4970 Size: 2158 39 281 532 255 279 1068 174 Cuts at: 4970 5361 5388 6733 184 Size: 391 27 1345 662 Fragments arranged by size: 2158 1345 1068 662 532 391 281 279 255 174 39 27 EagI C'GGCC_G Cuts at: 2381 4796 2381 Size: 2415 4796 EarI CTCTTCn'nnn_ Cuts at: 409 445 1983 2408 5234 5444 409 Size: 36 1538 425 2826 210 2176 Fragments arranged by size: 2826 2176 1538 425 210 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 156 1267 5419 5729 5875 7021 156 Size: 1111 4152 310 146 1146 346 Fragments arranged by size: 4152 1146 1111 346 310 146 EcoICRI GAG'CTC Cuts at: 4223 4223 Size: 7211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 265 274 1205 1249 1840 1916 2243 2440 3030 Size: 9 931 44 591 76 327 197 590 Cuts at: 3030 3904 4009 5036 5468 6215 6483 265 Size: 874 105 1027 432 747 268 993 Fragments arranged by size: 1027 993 931 874 747 591 590 432 327 268 197 105 76 44 9 EcoNI CCTnn'n_nnAGG Cuts at: 1079 1336 1079 Size: 257 6954 EcoO109I rG'GnC_Cy Cuts at: 131 645 1084 2197 4350 131 Size: 514 439 1113 2153 2992 Fragments arranged by size: 2992 2153 1113 514 439 EcoRI G'AATT_C Cuts at: 4215 4215 Size: 7211 EcoRV GAT'ATC Cuts at: 2514 3582 2514 Size: 1068 6143 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5230 5230 Size: 7211 FspI TGC'GCA Cuts at: 3841 4992 3841 Size: 1151 6060 FspAI rTGC'GCAy Cuts at: 3841 3841 Size: 7211 HaeII r_GCGC'y Cuts at: 252 933 971 1521 2558 3053 4645 4893 5915 Size: 681 38 550 1037 495 1592 248 1022 Cuts at: 5915 6894 252 Size: 979 569 Fragments arranged by size: 1592 1037 1022 979 681 569 550 495 248 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 462 494 583 615 1154 1186 2904 2936 3676 Size: 32 89 32 539 32 1718 32 740 Cuts at: 3676 3708 4048 4080 5053 5083 5085 5115 462 Size: 32 340 32 973 30 2 30 2558 Fragments arranged by size: 2558 1718 973 740 539 340 89 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 4250 4250 Size: 7211 HindIII A'AGCT_T Cuts at: 3254 3254 Size: 7211 KasI G'GCGC_C Cuts at: 1517 4641 4889 1517 Size: 3124 248 3839 Fragments arranged by size: 3839 3124 248 KpnI G_GTAC'C Cuts at: 1644 4231 1644 Size: 2587 4624 MfeI C'AATT_G Cuts at: 3276 3276 Size: 7211 MluI A'CGCG_T Cuts at: 6644 6644 Size: 7211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1224 1769 2585 3779 5882 6066 1224 Size: 545 816 1194 2103 184 2369 Fragments arranged by size: 2369 2103 1194 816 545 184 MscI TGG'CCA Cuts at: 2344 3196 4972 2344 Size: 852 1776 4583 Fragments arranged by size: 4583 1776 852 MslI CAynn'nnrTG Cuts at: 1252 1286 1870 1889 2299 2338 2353 5200 5327 Size: 34 584 19 410 39 15 2847 127 Cuts at: 5327 1252 Size: 3136 Fragments arranged by size: 3136 2847 584 410 127 39 34 19 15 MspA1I CmG'CkG Cuts at: 243 428 719 737 821 1751 2055 2522 4185 Size: 185 291 18 84 930 304 467 1663 Cuts at: 4185 4322 4636 4996 6009 6254 243 Size: 137 314 360 1013 245 1200 Fragments arranged by size: 1663 1200 1013 930 467 360 314 304 291 245 185 137 84 18 NaeI GCC'GGC Cuts at: 5392 5392 Size: 7211 NarI GG'CG_CC Cuts at: 1518 4642 4890 1518 Size: 3124 248 3839 Fragments arranged by size: 3839 3124 248 NcoI C'CATG_G Cuts at: 662 1868 662 Size: 1206 6005 NgoMIV G'CCGG_C Cuts at: 5390 5390 Size: 7211 NspI r_CATG'y Cuts at: 1020 1065 3228 3825 3846 4264 4764 5671 1020 Size: 45 2163 597 21 418 500 907 2560 Fragments arranged by size: 2560 2163 907 597 500 418 45 21 PasI CC'CwG_GG Cuts at: 4096 4096 Size: 7211 PciI A'CATG_T Cuts at: 5667 5667 Size: 7211 PflMI CCAn_nnn'nTGG Cuts at: 1751 1751 Size: 7211 PfoI T'CCnGG_A Cuts at: 587 3881 3926 587 Size: 3294 45 3872 Fragments arranged by size: 3872 3294 45 PmeI GTTT'AAAC Cuts at: 4210 4210 Size: 7211 PmlI CAC'GTG Cuts at: 761 761 Size: 7211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1993 2025 6350 6382 1993 Size: 32 4325 32 2822 Fragments arranged by size: 4325 2822 32 32 PpuMI rG'GwC_Cy Cuts at: 131 645 1084 131 Size: 514 439 6258 Fragments arranged by size: 6258 514 439 PshAI GACnn'nnGTC Cuts at: 650 3812 650 Size: 3162 4049 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3025 3057 3025 Size: 32 7179 PstI C_TGCA'G Cuts at: 826 2554 4258 4943 826 Size: 1728 1704 685 3094 Fragments arranged by size: 3094 1728 1704 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 7211 PvuII CAG'CTG Cuts at: 243 821 1751 2522 4636 4996 243 Size: 578 930 771 2114 360 2458 Fragments arranged by size: 2458 2114 930 771 578 360 RsrII CG'GwC_CG Cuts at: 5406 5406 Size: 7211 SacI G_AGCT'C Cuts at: 4225 4225 Size: 7211 SacII CC_GC'GG Cuts at: 429 429 Size: 7211 SalI G'TCGA_C Cuts at: 4248 4248 Size: 7211 SanDI GG'GwC_CC Cuts at: 131 131 Size: 7211 SapI GCTCTTCn'nnn_ Cuts at: 2408 5234 5444 2408 Size: 2826 210 4175 Fragments arranged by size: 4175 2826 210 SbfI CC_TGCA'GG Cuts at: 4258 4258 Size: 7211 ScaI AGT'ACT Cuts at: 358 3043 6728 358 Size: 2685 3685 841 Fragments arranged by size: 3685 2685 841 SfcI C'TryA_G Cuts at: 33 822 912 1656 2550 3945 4254 4939 5932 Size: 789 90 744 894 1395 309 685 993 Cuts at: 5932 6123 33 Size: 191 1121 Fragments arranged by size: 1395 1121 993 894 789 744 685 309 191 90 SfoI GGC'GCC Cuts at: 1519 4643 4891 1519 Size: 3124 248 3839 Fragments arranged by size: 3839 3124 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7211 SmaI CCC'GGG Cuts at: 2801 4233 2801 Size: 1432 5779 SmlI C'TyrA_G Cuts at: 385 481 3655 4366 5281 5773 6035 385 Size: 96 3174 711 915 492 262 1561 Fragments arranged by size: 3174 1561 915 711 492 262 96 SpeI A'CTAG_T Cuts at: 67 67 Size: 7211 SphI G_CATG'C Cuts at: 1020 3228 4264 4764 1020 Size: 2208 1036 500 3467 Fragments arranged by size: 3467 2208 1036 500 StuI AGG'CCT Cuts at: 2637 3873 2637 Size: 1236 5975 StyI C'CwwG_G Cuts at: 302 662 1158 1292 1332 1868 1949 2632 3158 Size: 360 496 134 40 536 81 683 526 Cuts at: 3158 3315 4345 4699 302 Size: 157 1030 354 2814 Fragments arranged by size: 2814 1030 683 536 526 496 360 354 157 134 81 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3292 4794 4927 5386 5595 3292 Size: 1502 133 459 209 4908 Fragments arranged by size: 4908 1502 459 209 133 TatI w'GTAC_w Cuts at: 356 707 1064 1445 1913 3041 6726 356 Size: 351 357 381 468 1128 3685 841 Fragments arranged by size: 3685 1128 841 468 381 357 351 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2942 2974 6728 6760 2942 Size: 32 3754 32 3393 Fragments arranged by size: 3754 3393 32 32 Tth111I GACn'n_nGTC Cuts at: 5008 5008 Size: 7211 XbaI T'CTAG_A Cuts at: 4242 4242 Size: 7211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1788 4706 1788 Size: 2918 4293 XhoI C'TCGA_G Cuts at: 385 385 Size: 7211 XmaI C'CCGG_G Cuts at: 2799 4231 2799 Size: 1432 5779 XmnI GAAnn'nnTTC Cuts at: 439 3271 439 Size: 2832 4379 ZraI GAC'GTC Cuts at: 1581 3687 1581 Size: 2106 5105 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AflII AlfI AloI ApaI AscI BaeI BfrBI BmtI BsiWI BsmI BsmBI BstEII BstZ17I CspCI FseI HpaI NdeI NheI NotI NruI NsiI PacI PsiI PspOMI PspXI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI