Restriction map for
pF1KB9097
(Circular) (Six-base) MAPSORT of: pF1KB9097.seq Check: 3440 from: 1 to: 7211
pF1KB9097 7211 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 1583 3689 1583
Size: 2106 5105
AccI GT'mk_AC
Cuts at: 4249 4249
Size: 7211
Acc65I G'GTAC_C
Cuts at: 1640 4227 1640
Size: 2587 4624
AclI AA'CG_TT
Cuts at: 7051 7051
Size: 7211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1205 1840 1916 2243 3030 5036 5468 6215
Size: 940 635 76 327 787 2006 432 747
Cuts at: 6215 6483 265
Size: 268 993
Fragments arranged by size:
2006 993 940 787 747 635 432 327
268 76
AfeI AGC'GCT
Cuts at: 3051 3051
Size: 7211
AflIII A'CryG_T
Cuts at: 760 4563 5667 6644 760
Size: 3803 1104 977 1327
Fragments arranged by size:
3803 1327 1104 977
AgeI A'CCGG_T
Cuts at: 1637 5599 1637
Size: 3962 3249
AhdI GACnn_n'nnGTC
Cuts at: 3690 3690
Size: 7211
AleI CACnn'nnGTG
Cuts at: 1870 1870
Size: 7211
AlwNI CAG_nnn'CTG
Cuts at: 243 1398 2863 4516 6083 243
Size: 1155 1465 1653 1567 1371
Fragments arranged by size:
1653 1567 1465 1371 1155
ApaLI G'TGCA_C
Cuts at: 777 5981 777
Size: 5204 2007
ApoI r'AATT_y
Cuts at: 1481 2277 4215 6564 6820 1481
Size: 796 1938 2349 256 1872
Fragments arranged by size:
2349 1938 1872 796 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7211
AvaI C'yCGr_G
Cuts at: 385 518 805 1032 2799 3089 3967 4231 385
Size: 133 287 227 1767 290 878 264 3365
Fragments arranged by size:
3365 1767 878 290 287 264 227 133
AvrII C'CTAG_G
Cuts at: 1332 1332
Size: 7211
BamHI G'GATC_C
Cuts at: 4236 4236
Size: 7211
BanI G'GyrC_C
Cuts at: 1517 1634 1640 2134 4227 4641 4889 4924 1517
Size: 117 6 494 2093 414 248 35 3804
Fragments arranged by size:
3804 2093 494 414 248 117 35 6
BanII G_rGCy'C
Cuts at: 155 1247 1799 4225 5255 155
Size: 1092 552 2426 1030 2111
Fragments arranged by size:
2426 2111 1092 1030 552
BbeI G_GCGC'C
Cuts at: 1521 4645 4893 1521
Size: 3124 248 3839
Fragments arranged by size:
3839 3124 248
BbsI GAAGACnn'nnnn_
Cuts at: 694 2967 3660 4187 694
Size: 2273 693 527 3718
Fragments arranged by size:
3718 2273 693 527
BbvCI CC'TCA_GC
Cuts at: 964 964
Size: 7211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 252 286 3751 3785 252
Size: 34 3465 34 3678
Fragments arranged by size:
3678 3465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5109 5870 7211 5109
Size: 761 1341 5109
Fragments arranged by size:
5109 1341 761
BclI T'GATC_A
Cuts at: 2912 4731 2912
Size: 1819 5392
BglI GCCn_nnn'nGGC
Cuts at: 1034 3727 1034
Size: 2693 4518
BglII A'GATC_T
Cuts at: 1907 4726 6763 1907
Size: 2819 2037 2355
Fragments arranged by size:
2819 2355 2037
BlpI GC'TnA_GC
Cuts at: 4323 4323
Size: 7211
Bme1580I G_kGCm'C
Cuts at: 754 781 1030 2306 4836 4929 5985 754
Size: 27 249 1276 2530 93 1056 1980
Fragments arranged by size:
2530 1980 1276 1056 249 93 27
BmgBI CAC'GTC
Cuts at: 1607 1767 1791 1607
Size: 160 24 7027
Fragments arranged by size:
7027 160 24
BmrI ACTGGGnnnn_n'
Cuts at: 1416 4185 4423 4504 4839 6983 1416
Size: 2769 238 81 335 2144 1644
Fragments arranged by size:
2769 2144 1644 335 238 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1047 1581 5578 1047
Size: 534 3997 2680
Fragments arranged by size:
3997 2680 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1249 2440 3904 4009 274
Size: 975 1191 1464 105 3476
Fragments arranged by size:
3476 1464 1191 975 105
Bpu10I CC'TnA_GC
Cuts at: 964 3430 964
Size: 2466 4745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 3640 4387 5266 5758 6056 466
Size: 3174 747 879 492 298 1621
Fragments arranged by size:
3174 1621 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 392 1302 3386 3500 3939 6915 392
Size: 910 2084 114 439 2976 688
Fragments arranged by size:
2976 2084 910 688 439 114
BsaAI yAC'GTr
Cuts at: 761 1269 4564 761
Size: 508 3295 3408
Fragments arranged by size:
3408 3295 508
BsaBI GATnn'nnATC
Cuts at: 6 4725 6762 6
Size: 4719 2037 455
Fragments arranged by size:
4719 2037 455
BsaHI Gr'CG_yC
Cuts at: 1518 1580 3686 4642 4890 5592 7088 1518
Size: 62 2106 956 248 702 1496 1641
Fragments arranged by size:
2106 1641 1496 956 702 248 62
BsaWI w'CCGG_w
Cuts at: 121 1637 3377 3602 4400 4921 5599 5873 6020
Size: 1516 1740 225 798 521 678 274 147
Cuts at: 6020 6431 121
Size: 411 901
Fragments arranged by size:
1740 1516 901 798 678 521 411 274
225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2413 2443 2942 2972 2413
Size: 30 499 30 6652
Fragments arranged by size:
6652 499 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 682 1354 1965 2164 2263 3922 3967 3991
Size: 542 672 611 199 99 1659 45 24
Cuts at: 3991 4023 140
Size: 32 3328
Fragments arranged by size:
3328 1659 672 611 542 199 99 45
32 24
BseYI C'CCAG_C
Cuts at: 2544 3003 3300 4636 5394 5971 6467 2544
Size: 459 297 1336 758 577 496 3288
Fragments arranged by size:
3288 1336 758 577 496 459 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 2269 246
Size: 2023 5188
BsiEI CG_ry'CG
Cuts at: 104 2384 4753 4799 6007 104
Size: 2280 2369 46 1208 1308
Fragments arranged by size:
2369 2280 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 781 4225 5003 5193 5985 781
Size: 3444 778 190 792 2007
Fragments arranged by size:
3444 2007 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 155 754 781 1030 1247 1799 2306 4225 4836
Size: 599 27 249 217 552 507 1919 611
Cuts at: 4836 4929 5003 5193 5255 5985 155
Size: 93 74 190 62 730 1381
Fragments arranged by size:
1919 1381 730 611 599 552 507 249
217 190 93 74 62 27
BspEI T'CCGG_A
Cuts at: 121 6431 121
Size: 6310 901
BspHI T'CATG_A
Cuts at: 6387 6387
Size: 7211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4210 4261 4475 4777 5158 4210
Size: 51 214 302 381 6263
Fragments arranged by size:
6263 381 302 214 51
BsrBI CCG'CTC
Cuts at: 50 4173 5503 5557 7041 7206 50
Size: 4123 1330 54 1484 165 55
Fragments arranged by size:
4123 1484 1330 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1214 5123 1214
Size: 3909 3302
BsrFI r'CCGG_y
Cuts at: 877 1637 1643 2081 2789 5209 5390 5599 877
Size: 760 6 438 708 2420 181 209 2489
Fragments arranged by size:
2489 2420 760 708 438 209 181 6
BsrGI T'GTAC_A
Cuts at: 1913 1913
Size: 7211
BssHII G'CGCG_C
Cuts at: 827 827
Size: 7211
BssSI C'ACGA_G
Cuts at: 2530 5482 5840 2530
Size: 2952 358 3901
Fragments arranged by size:
3901 2952 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1067 4588 1067
Size: 3521 3690
BstBI TT'CG_AA
Cuts at: 5572 5572
Size: 7211
BstXI CCAn_nnnn'nTGG
Cuts at: 1891 1950 2624 3165 1891
Size: 59 674 541 5937
Fragments arranged by size:
5937 674 541 59
BstYI r'GATC_y
Cuts at: 1 1274 1662 1907 2859 4236 4703 4726 5061
Size: 1273 388 245 952 1377 467 23 335
Cuts at: 5061 5307 6319 6405 6417 6763 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1377 1273 1012 952 467 449 388 346
335 246 245 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 505 2063 505
Size: 1558 5653
BtgI C'CryG_G
Cuts at: 426 662 1868 1890 2378 426
Size: 236 1206 22 488 5259
Fragments arranged by size:
5259 1206 488 236 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2901 5161 5342 5505 2901
Size: 2260 181 163 4607
Fragments arranged by size:
4607 2260 181 163
BtsI GCAGTG_nn'
Cuts at: 4048 4454 4048
Size: 406 6805
ClaI AT'CG_AT
Cuts at: 2510 2510
Size: 7211
DraI TTT'AAA
Cuts at: 2657 4210 2657
Size: 1553 5658
DraIII CAC_nnn'GTG
Cuts at: 3288 3288
Size: 7211
DrdI GACnn_nn'nnGTC
Cuts at: 4917 5775 4917
Size: 858 6353
EaeI y'GGCC_r
Cuts at: 184 2342 2381 2662 3194 3449 3728 4796 4970
Size: 2158 39 281 532 255 279 1068 174
Cuts at: 4970 5361 5388 6733 184
Size: 391 27 1345 662
Fragments arranged by size:
2158 1345 1068 662 532 391 281 279
255 174 39 27
EagI C'GGCC_G
Cuts at: 2381 4796 2381
Size: 2415 4796
EarI CTCTTCn'nnn_
Cuts at: 409 445 1983 2408 5234 5444 409
Size: 36 1538 425 2826 210 2176
Fragments arranged by size:
2826 2176 1538 425 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 156 1267 5419 5729 5875 7021 156
Size: 1111 4152 310 146 1146 346
Fragments arranged by size:
4152 1146 1111 346 310 146
EcoICRI GAG'CTC
Cuts at: 4223 4223
Size: 7211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 274 1205 1249 1840 1916 2243 2440 3030
Size: 9 931 44 591 76 327 197 590
Cuts at: 3030 3904 4009 5036 5468 6215 6483 265
Size: 874 105 1027 432 747 268 993
Fragments arranged by size:
1027 993 931 874 747 591 590 432
327 268 197 105 76 44 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1079 1336 1079
Size: 257 6954
EcoO109I rG'GnC_Cy
Cuts at: 131 645 1084 2197 4350 131
Size: 514 439 1113 2153 2992
Fragments arranged by size:
2992 2153 1113 514 439
EcoRI G'AATT_C
Cuts at: 4215 4215
Size: 7211
EcoRV GAT'ATC
Cuts at: 2514 3582 2514
Size: 1068 6143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5230 5230
Size: 7211
FspI TGC'GCA
Cuts at: 3841 4992 3841
Size: 1151 6060
FspAI rTGC'GCAy
Cuts at: 3841 3841
Size: 7211
HaeII r_GCGC'y
Cuts at: 252 933 971 1521 2558 3053 4645 4893 5915
Size: 681 38 550 1037 495 1592 248 1022
Cuts at: 5915 6894 252
Size: 979 569
Fragments arranged by size:
1592 1037 1022 979 681 569 550 495
248 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 462 494 583 615 1154 1186 2904 2936 3676
Size: 32 89 32 539 32 1718 32 740
Cuts at: 3676 3708 4048 4080 5053 5083 5085 5115 462
Size: 32 340 32 973 30 2 30 2558
Fragments arranged by size:
2558 1718 973 740 539 340 89 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4250 4250
Size: 7211
HindIII A'AGCT_T
Cuts at: 3254 3254
Size: 7211
KasI G'GCGC_C
Cuts at: 1517 4641 4889 1517
Size: 3124 248 3839
Fragments arranged by size:
3839 3124 248
KpnI G_GTAC'C
Cuts at: 1644 4231 1644
Size: 2587 4624
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 7211
MluI A'CGCG_T
Cuts at: 6644 6644
Size: 7211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1224 1769 2585 3779 5882 6066 1224
Size: 545 816 1194 2103 184 2369
Fragments arranged by size:
2369 2103 1194 816 545 184
MscI TGG'CCA
Cuts at: 2344 3196 4972 2344
Size: 852 1776 4583
Fragments arranged by size:
4583 1776 852
MslI CAynn'nnrTG
Cuts at: 1252 1286 1870 1889 2299 2338 2353 5200 5327
Size: 34 584 19 410 39 15 2847 127
Cuts at: 5327 1252
Size: 3136
Fragments arranged by size:
3136 2847 584 410 127 39 34 19
15
MspA1I CmG'CkG
Cuts at: 243 428 719 737 821 1751 2055 2522 4185
Size: 185 291 18 84 930 304 467 1663
Cuts at: 4185 4322 4636 4996 6009 6254 243
Size: 137 314 360 1013 245 1200
Fragments arranged by size:
1663 1200 1013 930 467 360 314 304
291 245 185 137 84 18
NaeI GCC'GGC
Cuts at: 5392 5392
Size: 7211
NarI GG'CG_CC
Cuts at: 1518 4642 4890 1518
Size: 3124 248 3839
Fragments arranged by size:
3839 3124 248
NcoI C'CATG_G
Cuts at: 662 1868 662
Size: 1206 6005
NgoMIV G'CCGG_C
Cuts at: 5390 5390
Size: 7211
NspI r_CATG'y
Cuts at: 1020 1065 3228 3825 3846 4264 4764 5671 1020
Size: 45 2163 597 21 418 500 907 2560
Fragments arranged by size:
2560 2163 907 597 500 418 45 21
PasI CC'CwG_GG
Cuts at: 4096 4096
Size: 7211
PciI A'CATG_T
Cuts at: 5667 5667
Size: 7211
PflMI CCAn_nnn'nTGG
Cuts at: 1751 1751
Size: 7211
PfoI T'CCnGG_A
Cuts at: 587 3881 3926 587
Size: 3294 45 3872
Fragments arranged by size:
3872 3294 45
PmeI GTTT'AAAC
Cuts at: 4210 4210
Size: 7211
PmlI CAC'GTG
Cuts at: 761 761
Size: 7211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1993 2025 6350 6382 1993
Size: 32 4325 32 2822
Fragments arranged by size:
4325 2822 32 32
PpuMI rG'GwC_Cy
Cuts at: 131 645 1084 131
Size: 514 439 6258
Fragments arranged by size:
6258 514 439
PshAI GACnn'nnGTC
Cuts at: 650 3812 650
Size: 3162 4049
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3025 3057 3025
Size: 32 7179
PstI C_TGCA'G
Cuts at: 826 2554 4258 4943 826
Size: 1728 1704 685 3094
Fragments arranged by size:
3094 1728 1704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7211
PvuII CAG'CTG
Cuts at: 243 821 1751 2522 4636 4996 243
Size: 578 930 771 2114 360 2458
Fragments arranged by size:
2458 2114 930 771 578 360
RsrII CG'GwC_CG
Cuts at: 5406 5406
Size: 7211
SacI G_AGCT'C
Cuts at: 4225 4225
Size: 7211
SacII CC_GC'GG
Cuts at: 429 429
Size: 7211
SalI G'TCGA_C
Cuts at: 4248 4248
Size: 7211
SanDI GG'GwC_CC
Cuts at: 131 131
Size: 7211
SapI GCTCTTCn'nnn_
Cuts at: 2408 5234 5444 2408
Size: 2826 210 4175
Fragments arranged by size:
4175 2826 210
SbfI CC_TGCA'GG
Cuts at: 4258 4258
Size: 7211
ScaI AGT'ACT
Cuts at: 358 3043 6728 358
Size: 2685 3685 841
Fragments arranged by size:
3685 2685 841
SfcI C'TryA_G
Cuts at: 33 822 912 1656 2550 3945 4254 4939 5932
Size: 789 90 744 894 1395 309 685 993
Cuts at: 5932 6123 33
Size: 191 1121
Fragments arranged by size:
1395 1121 993 894 789 744 685 309
191 90
SfoI GGC'GCC
Cuts at: 1519 4643 4891 1519
Size: 3124 248 3839
Fragments arranged by size:
3839 3124 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7211
SmaI CCC'GGG
Cuts at: 2801 4233 2801
Size: 1432 5779
SmlI C'TyrA_G
Cuts at: 385 481 3655 4366 5281 5773 6035 385
Size: 96 3174 711 915 492 262 1561
Fragments arranged by size:
3174 1561 915 711 492 262 96
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7211
SphI G_CATG'C
Cuts at: 1020 3228 4264 4764 1020
Size: 2208 1036 500 3467
Fragments arranged by size:
3467 2208 1036 500
StuI AGG'CCT
Cuts at: 2637 3873 2637
Size: 1236 5975
StyI C'CwwG_G
Cuts at: 302 662 1158 1292 1332 1868 1949 2632 3158
Size: 360 496 134 40 536 81 683 526
Cuts at: 3158 3315 4345 4699 302
Size: 157 1030 354 2814
Fragments arranged by size:
2814 1030 683 536 526 496 360 354
157 134 81 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3292 4794 4927 5386 5595 3292
Size: 1502 133 459 209 4908
Fragments arranged by size:
4908 1502 459 209 133
TatI w'GTAC_w
Cuts at: 356 707 1064 1445 1913 3041 6726 356
Size: 351 357 381 468 1128 3685 841
Fragments arranged by size:
3685 1128 841 468 381 357 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 6728 6760 2942
Size: 32 3754 32 3393
Fragments arranged by size:
3754 3393 32 32
Tth111I GACn'n_nGTC
Cuts at: 5008 5008
Size: 7211
XbaI T'CTAG_A
Cuts at: 4242 4242
Size: 7211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1788 4706 1788
Size: 2918 4293
XhoI C'TCGA_G
Cuts at: 385 385
Size: 7211
XmaI C'CCGG_G
Cuts at: 2799 4231 2799
Size: 1432 5779
XmnI GAAnn'nnTTC
Cuts at: 439 3271 439
Size: 2832 4379
ZraI GAC'GTC
Cuts at: 1581 3687 1581
Size: 2106 5105
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AlfI AloI ApaI AscI BaeI BfrBI
BmtI BsiWI BsmI BsmBI BstEII BstZ17I CspCI FseI
HpaI NdeI NheI NotI NruI NsiI PacI PsiI
PspOMI PspXI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI