Restriction map for
pF1KB9098
(Circular) (Six-base) MAPSORT of: pF1KB9098.seq Check: 488 from: 1 to: 6884
pF1KB9098 6884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 551 551
Size: 6884
AatII G_ACGT'C
Cuts at: 965 965
Size: 6884
AccI GT'mk_AC
Cuts at: 3922 3922
Size: 6884
Acc65I G'GTAC_C
Cuts at: 3900 3900
Size: 6884
AclI AA'CG_TT
Cuts at: 6724 6724
Size: 6884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 649 1048 1207 1959 2418 2522 2663 3158
Size: 262 399 159 752 459 104 141 495
Cuts at: 3158 3326 4709 5141 5494 6156 387
Size: 168 1383 432 353 662 1115
Fragments arranged by size:
1383 1115 752 662 495 459 432 399
353 262 168 159 141 104
AfeI AGC'GCT
Cuts at: 2543 2543
Size: 6884
AflIII A'CryG_T
Cuts at: 4236 6036 6317 4236
Size: 1800 281 4803
Fragments arranged by size:
4803 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2726 2726
Size: 6884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2020 2020
Size: 6884
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 6852
AlwNI CAG_nnn'CTG
Cuts at: 255 882 1146 4189 5627 255
Size: 627 264 3043 1438 1512
Fragments arranged by size:
3043 1512 1438 627 264
ApaLI G'TGCA_C
Cuts at: 5722 5722
Size: 6884
ApoI r'AATT_y
Cuts at: 1902 2673 3888 6237 6493 1902
Size: 771 1215 2349 256 2293
Fragments arranged by size:
2349 2293 1215 771 256
AseI AT'TA_AT
Cuts at: 20 523 20
Size: 503 6381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6884
AvaI C'yCGr_G
Cuts at: 279 294 3904 279
Size: 15 3610 3259
Fragments arranged by size:
3610 3259 15
BamHI G'GATC_C
Cuts at: 119 1720 3909 119
Size: 1601 2189 3094
Fragments arranged by size:
3094 2189 1601
BanI G'GyrC_C
Cuts at: 1503 1538 2806 3900 4314 4562 4597 1503
Size: 35 1268 1094 414 248 35 3790
Fragments arranged by size:
3790 1268 1094 414 248 35 35
BanII G_rGCy'C
Cuts at: 286 1292 1880 2195 2560 2984 3035 3158 3898
Size: 1006 588 315 365 424 51 123 740
Cuts at: 3898 4928 286
Size: 1030 2242
Fragments arranged by size:
2242 1030 1006 740 588 424 365 315
123 51
BbeI G_GCGC'C
Cuts at: 1542 4318 4566 1542
Size: 2776 248 3860
Fragments arranged by size:
3860 2776 248
BbsI GAAGACnn'nnnn_
Cuts at: 574 710 781 2100 2537 3210 3692 3839 574
Size: 136 71 1319 437 673 482 147 3619
Fragments arranged by size:
3619 1319 673 482 437 147 136 71
BbvCI CC'TCA_GC
Cuts at: 433 860 2383 433
Size: 427 1523 4934
Fragments arranged by size:
4934 1523 427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1419 1453 2979 3013 90
Size: 34 1295 34 1526 34 3961
Fragments arranged by size:
3961 1526 1295 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4782 5838 6884 4782
Size: 1056 1046 4782
Fragments arranged by size:
4782 1056 1046
BclI T'GATC_A
Cuts at: 1433 1928 2592 4404 1433
Size: 495 664 1812 3913
Fragments arranged by size:
3913 1812 664 495
BglI GCCn_nnn'nGGC
Cuts at: 297 2761 297
Size: 2464 4420
BglII A'GATC_T
Cuts at: 1010 4399 6436 1010
Size: 3389 2037 1458
Fragments arranged by size:
3389 2037 1458
BlpI GC'TnA_GC
Cuts at: 2545 3152 3996 2545
Size: 607 844 5433
Fragments arranged by size:
5433 844 607
Bme1580I G_kGCm'C
Cuts at: 1508 2809 4509 4602 5726 1508
Size: 1301 1700 93 1124 2666
Fragments arranged by size:
2666 1700 1301 1124 93
BmgBI CAC'GTC
Cuts at: 141 3500 141
Size: 3359 3525
BmrI ACTGGGnnnn_n'
Cuts at: 2949 4096 4177 4512 6656 2949
Size: 1147 81 335 2144 3177
Fragments arranged by size:
3177 2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 294 3474 5251 294
Size: 3180 1777 1927
Fragments arranged by size:
3180 1927 1777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 394 874 1026 1249 1375 1646 2059 2209
Size: 63 480 152 223 126 271 413 150
Cuts at: 2209 3136 3199 3469 331
Size: 927 63 270 3746
Fragments arranged by size:
3746 927 480 413 271 270 223 152
150 126 63 63
Bpu10I CC'TnA_GC
Cuts at: 193 433 860 2065 2383 2841 193
Size: 240 427 1205 318 458 4236
Fragments arranged by size:
4236 1205 458 427 318 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 4060 4939 5653 5951 637
Size: 3423 879 714 298 1570
Fragments arranged by size:
3423 1570 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1225 1553 2962 6588 1225
Size: 328 1409 3626 1521
Fragments arranged by size:
3626 1521 1409 328
BsaAI yAC'GTr
Cuts at: 873 4237 873
Size: 3364 3520
BsaBI GATnn'nnATC
Cuts at: 6 616 2591 4398 6435 6
Size: 610 1975 1807 2037 455
Fragments arranged by size:
2037 1975 1807 610 455
BsaHI Gr'CG_yC
Cuts at: 962 1539 1940 3670 4315 4563 5265 6761 962
Size: 577 401 1730 645 248 702 1496 1085
Fragments arranged by size:
1730 1496 1085 702 645 577 401 248
BsaWI w'CCGG_w
Cuts at: 797 1406 2828 4073 4594 5272 5683 5830 6104
Size: 609 1422 1245 521 678 411 147 274
Cuts at: 6104 797
Size: 1577
Fragments arranged by size:
1577 1422 1245 678 609 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1348 1378 2182 2212 2625 2655 3854 3884 1348
Size: 30 804 30 413 30 1199 30 4348
Fragments arranged by size:
4348 1199 804 413 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1231 1372 1702 1720 2262 2593 3133 3253 3676
Size: 141 330 18 542 331 540 120 423
Cuts at: 3676 1231
Size: 4439
Fragments arranged by size:
4439 542 540 423 331 330 141 120
18
BseYI C'CCAG_C
Cuts at: 4309 5067 5732 6140 4309
Size: 758 665 408 5053
Fragments arranged by size:
5053 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 754 868 895 1659 2077 2870 523
Size: 231 114 27 764 418 793 4537
Fragments arranged by size:
4537 793 764 418 231 114 27
BsiEI CG_ry'CG
Cuts at: 104 330 2765 4426 4472 5702 104
Size: 226 2435 1661 46 1230 1286
Fragments arranged by size:
2435 1661 1286 1230 226 46
BsiHKAI G_wGCw'C
Cuts at: 286 1292 1316 2195 2560 2933 2984 3035 3779
Size: 1006 24 879 365 373 51 51 744
Cuts at: 3779 3898 4676 4866 5726 286
Size: 119 778 190 860 1444
Fragments arranged by size:
1444 1006 879 860 778 744 373 365
190 119 51 51 24
BsmBI CGTCTCn'nnnn_
Cuts at: 1387 1387
Size: 6884
Bsp1286I G_dGCh'C
Cuts at: 286 1292 1316 1508 1880 2195 2560 2809 2933
Size: 1006 24 192 372 315 365 249 124
Cuts at: 2933 2984 3035 3158 3779 3898 4509 4602 4676
Size: 51 51 123 621 119 611 93 74
Cuts at: 4676 4866 4928 5726 286
Size: 190 62 798 1444
Fragments arranged by size:
1444 1006 798 621 611 372 365 315
249 192 190 124 123 119 93 74
62 51 51 24
BspEI T'CCGG_A
Cuts at: 1406 1406
Size: 6884
BspHI T'CATG_A
Cuts at: 1308 1880 2717 3296 5316 1308
Size: 572 837 579 2020 2876
Fragments arranged by size:
2876 2020 837 579 572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 314 545 551 2063 3934 4148 4450 4831
Size: 51 231 6 1512 1871 214 302 381
Cuts at: 4831 263
Size: 2316
Fragments arranged by size:
2316 1871 1512 381 302 231 214 51
6
BsrBI CCG'CTC
Cuts at: 50 624 2031 3048 5176 5230 6714 6879 50
Size: 574 1407 1017 2128 54 1484 165 55
Fragments arranged by size:
2128 1484 1407 1017 574 165 55 54
BsrDI GCAATG_nn'
Cuts at: 476 1878 3363 4796 476
Size: 1402 1485 1433 2564
Fragments arranged by size:
2564 1485 1433 1402
BsrFI r'CCGG_y
Cuts at: 348 761 2318 4882 5063 348
Size: 413 1557 2564 181 2169
Fragments arranged by size:
2564 2169 1557 413 181
BssHII G'CGCG_C
Cuts at: 163 332 2253 163
Size: 169 1921 4794
Fragments arranged by size:
4794 1921 169
BssSI C'ACGA_G
Cuts at: 5155 5863 5155
Size: 708 6176
BstAPI GCAn_nnn'nTGC
Cuts at: 255 478 546 882 4261 255
Size: 223 68 336 3379 2878
Fragments arranged by size:
3379 2878 336 223 68
BstBI TT'CG_AA
Cuts at: 5245 5245
Size: 6884
BstEII G'GTnAC_C
Cuts at: 800 800
Size: 6884
BstXI CCAn_nnnn'nTGG
Cuts at: 2187 2875 2187
Size: 688 6196
BstYI r'GATC_y
Cuts at: 1 119 1010 1142 1259 1451 1720 2009 3909
Size: 118 891 132 117 192 269 289 1900
Cuts at: 3909 4376 4399 4734 4980 5286 5298 5384 6436
Size: 467 23 335 246 306 12 86 1052
Cuts at: 6436 1
Size: 449
Fragments arranged by size:
1900 1052 891 467 449 335 306 289
269 246 192 132 118 117 86 23
12
BtgI C'CryG_G
Cuts at: 572 572
Size: 6884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4834 5015 5178 4834
Size: 181 163 6540
Fragments arranged by size:
6540 181 163
BtsI GCAGTG_nn'
Cuts at: 1152 2757 3480 4127 1152
Size: 1605 723 647 3909
Fragments arranged by size:
3909 1605 723 647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 214 249 214
Size: 35 6849
DraI TTT'AAA
Cuts at: 1752 3883 1752
Size: 2131 4753
DraIII CAC_nnn'GTG
Cuts at: 2943 3616 2943
Size: 673 6211
DrdI GACnn_nn'nnGTC
Cuts at: 3694 4590 5934 3694
Size: 896 1344 4644
Fragments arranged by size:
4644 1344 896
EaeI y'GGCC_r
Cuts at: 135 359 575 923 1418 1725 2208 2375 2420
Size: 224 216 348 495 307 483 167 45
Cuts at: 2420 2762 3401 4469 4643 5034 5061 6406 135
Size: 342 639 1068 174 391 27 1345 613
Fragments arranged by size:
1345 1068 639 613 495 483 391 348
342 307 224 216 174 167 45 27
EagI C'GGCC_G
Cuts at: 2762 4469 2762
Size: 1707 5177
EarI CTCTTCn'nnn_
Cuts at: 265 4907 5117 265
Size: 4642 210 2032
Fragments arranged by size:
4642 2032 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 780 2337 2923 5092 5834 5980 6694 780
Size: 1557 586 2169 742 146 714 970
Fragments arranged by size:
2169 1557 970 742 714 586 146
EcoICRI GAG'CTC
Cuts at: 284 1290 2193 2558 2982 3033 3896 284
Size: 1006 903 365 424 51 863 3272
Fragments arranged by size:
3272 1006 903 863 424 365 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 387 394 649 874 1026 1048 1207 1249
Size: 56 7 255 225 152 22 159 42
Cuts at: 1249 1375 1646 1959 2059 2209 2418 2522 2663
Size: 126 271 313 100 150 209 104 141
Cuts at: 2663 3136 3158 3199 3326 3469 4709 5141 5494
Size: 473 22 41 127 143 1240 432 353
Cuts at: 5494 6156 331
Size: 662 1059
Fragments arranged by size:
1240 1059 662 473 432 353 313 271
255 225 209 159 152 150 143 141
127 126 104 100 56 42 41 22
22 7
EcoNI CCTnn'n_nnAGG
Cuts at: 310 652 1498 3040 310
Size: 342 846 1542 4154
Fragments arranged by size:
4154 1542 846 342
EcoO109I rG'GnC_Cy
Cuts at: 497 2364 3090 3284 4023 497
Size: 1867 726 194 739 3358
Fragments arranged by size:
3358 1867 739 726 194
EcoRI G'AATT_C
Cuts at: 3888 3888
Size: 6884
EcoRV GAT'ATC
Cuts at: 3722 3722
Size: 6884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1656 3305 4903 1656
Size: 1649 1598 3637
Fragments arranged by size:
3637 1649 1598
FspI TGC'GCA
Cuts at: 823 4665 823
Size: 3842 3042
HaeII r_GCGC'y
Cuts at: 355 1542 2545 3386 4318 4566 5796 6567 355
Size: 1187 1003 841 932 248 1230 771 672
Fragments arranged by size:
1230 1187 1003 932 841 771 672 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 628 660 1348 1380 2712 2744 4726
Size: 32 328 32 688 32 1332 32 1982
Cuts at: 4726 4756 4758 4788 268
Size: 30 2 30 2364
Fragments arranged by size:
2364 1982 1332 688 328 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 897 1965 3699 3923 897
Size: 1068 1734 224 3858
Fragments arranged by size:
3858 1734 1068 224
HindIII A'AGCT_T
Cuts at: 1633 3527 3550 1633
Size: 1894 23 4967
Fragments arranged by size:
4967 1894 23
KasI G'GCGC_C
Cuts at: 1538 4314 4562 1538
Size: 2776 248 3860
Fragments arranged by size:
3860 2776 248
KpnI G_GTAC'C
Cuts at: 3904 3904
Size: 6884
MluI A'CGCG_T
Cuts at: 6317 6317
Size: 6884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1961 2668 5643 5827 1961
Size: 707 2975 184 3018
Fragments arranged by size:
3018 2975 707 184
MscI TGG'CCA
Cuts at: 137 577 1420 2210 4645 137
Size: 440 843 790 2435 2376
Fragments arranged by size:
2435 2376 843 790 440
MslI CAynn'nnrTG
Cuts at: 1501 1798 2410 3130 3317 4873 5000 1501
Size: 297 612 720 187 1556 127 3385
Fragments arranged by size:
3385 1556 720 612 297 187 127
MspA1I CmG'CkG
Cuts at: 372 882 915 1046 2387 3195 3422 3995 4309
Size: 510 33 131 1341 808 227 573 314
Cuts at: 4309 4669 5453 5698 372
Size: 360 784 245 1558
Fragments arranged by size:
1558 1341 808 784 573 510 360 314
245 227 131 33
NaeI GCC'GGC
Cuts at: 350 763 2320 5065 350
Size: 413 1557 2745 2169
Fragments arranged by size:
2745 2169 1557 413
NarI GG'CG_CC
Cuts at: 1539 4315 4563 1539
Size: 2776 248 3860
Fragments arranged by size:
3860 2776 248
NdeI CA'TA_TG
Cuts at: 3415 3415
Size: 6884
NgoMIV G'CCGG_C
Cuts at: 348 761 2318 5063 348
Size: 413 1557 2745 2169
Fragments arranged by size:
2745 2169 1557 413
NspI r_CATG'y
Cuts at: 487 822 852 2415 3937 4437 6040 487
Size: 335 30 1563 1522 500 1603 1331
Fragments arranged by size:
1603 1563 1522 1331 500 335 30
PasI CC'CwG_GG
Cuts at: 150 150
Size: 6884
PciI A'CATG_T
Cuts at: 6036 6036
Size: 6884
PflMI CCAn_nnn'nTGG
Cuts at: 1854 1854
Size: 6884
PfoI T'CCnGG_A
Cuts at: 785 1457 2129 3005 785
Size: 672 672 876 4664
Fragments arranged by size:
4664 876 672 672
PmeI GTTT'AAAC
Cuts at: 3883 3883
Size: 6884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1473 1505 3854 3886 5330 5362 1473
Size: 32 2349 32 1444 32 2995
Fragments arranged by size:
2995 2349 1444 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 497 3090 3284 497
Size: 2593 194 4097
Fragments arranged by size:
4097 2593 194
PspXI vC'TCGA_Gb
Cuts at: 279 279
Size: 6884
PstI C_TGCA'G
Cuts at: 251 551 1142 2009 3060 3931 4616 251
Size: 300 591 867 1051 871 685 2519
Fragments arranged by size:
2519 1051 871 867 685 591 300
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6884
PvuII CAG'CTG
Cuts at: 882 1046 2387 3195 4309 4669 882
Size: 164 1341 808 1114 360 3097
Fragments arranged by size:
3097 1341 1114 808 360 164
RsrII CG'GwC_CG
Cuts at: 5079 5079
Size: 6884
SacI G_AGCT'C
Cuts at: 286 1292 2195 2560 2984 3035 3898 286
Size: 1006 903 365 424 51 863 3272
Fragments arranged by size:
3272 1006 903 863 424 365 51
SalI G'TCGA_C
Cuts at: 3921 3921
Size: 6884
SapI GCTCTTCn'nnn_
Cuts at: 265 4907 5117 265
Size: 4642 210 2032
Fragments arranged by size:
4642 2032 210
SbfI CC_TGCA'GG
Cuts at: 3931 3931
Size: 6884
ScaI AGT'ACT
Cuts at: 6401 6401
Size: 6884
SexAI A'CCwGG_T
Cuts at: 833 833
Size: 6884
SfcI C'TryA_G
Cuts at: 33 247 492 547 1138 2005 3056 3927 4612
Size: 214 245 55 591 867 1051 871 685
Cuts at: 4612 5580 5771 33
Size: 968 191 1146
Fragments arranged by size:
1146 1051 968 871 867 685 591 245
214 191 55
SfoI GGC'GCC
Cuts at: 1540 4316 4564 1540
Size: 2776 248 3860
Fragments arranged by size:
3860 2776 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6884
SgrAI Cr'CCGG_yG
Cuts at: 2318 2318
Size: 6884
SmaI CCC'GGG
Cuts at: 3906 3906
Size: 6884
SmlI C'TyrA_G
Cuts at: 279 652 4039 4954 5668 5930 279
Size: 373 3387 915 714 262 1233
Fragments arranged by size:
3387 1233 915 714 373 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6884
SphI G_CATG'C
Cuts at: 822 852 3937 4437 822
Size: 30 3085 500 3269
Fragments arranged by size:
3269 3085 500 30
SspI AAT'ATT
Cuts at: 3799 3799
Size: 6884
StuI AGG'CCT
Cuts at: 1025 1025
Size: 6884
StyI C'CwwG_G
Cuts at: 362 425 1020 1907 4018 4372 362
Size: 63 595 887 2111 354 2874
Fragments arranged by size:
2874 2111 887 595 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 456 694 2667 2931 3387 4467 4600 5059 5268
Size: 238 1973 264 456 1080 133 459 209
Cuts at: 5268 456
Size: 2072
Fragments arranged by size:
2072 1973 1080 459 456 264 238 209
133
TatI w'GTAC_w
Cuts at: 1130 2414 6399 1130
Size: 1284 3985 1615
Fragments arranged by size:
3985 1615 1284
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 162 2929 2961 3602 3634 6401 6433 130
Size: 32 2767 32 641 32 2767 32 581
Fragments arranged by size:
2767 2767 641 581 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 961 2304 4681 961
Size: 1343 2377 3164
Fragments arranged by size:
3164 2377 1343
XbaI T'CTAG_A
Cuts at: 3915 3915
Size: 6884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 1059 1350 1539 2121 2127 2608 4379 369
Size: 690 291 189 582 6 481 1771 2874
Fragments arranged by size:
2874 1771 690 582 481 291 189 6
XhoI C'TCGA_G
Cuts at: 279 279
Size: 6884
XmaI C'CCGG_G
Cuts at: 3904 3904
Size: 6884
ZraI GAC'GTC
Cuts at: 963 963
Size: 6884
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AleI ApaI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsrGI BstZ17I Bsu36I ClaI FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SfiI SnaBI SrfI SwaI XmnI