Restriction map for
pF1KB9116
(Circular) (Six-base) MAPSORT of: pF1KB9116.seq Check: 5492 from: 1 to: 5429
pF1KB9116 5429 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 2467 2467
Size: 5429
Acc65I G'GTAC_C
Cuts at: 2445 2445
Size: 5429
AclI AA'CG_TT
Cuts at: 1300 5269 1300
Size: 3969 1460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2264 3254 3686 4433 4701 2228
Size: 36 990 432 747 268 2956
Fragments arranged by size:
2956 990 747 432 268 36
AflIII A'CryG_T
Cuts at: 2781 3885 4862 2781
Size: 1104 977 3348
Fragments arranged by size:
3348 1104 977
AgeI A'CCGG_T
Cuts at: 3817 3817
Size: 5429
AleI CACnn'nnGTG
Cuts at: 2046 2046
Size: 5429
AlwNI CAG_nnn'CTG
Cuts at: 2310 2734 4301 2310
Size: 424 1567 3438
Fragments arranged by size:
3438 1567 424
ApaLI G'TGCA_C
Cuts at: 4199 4199
Size: 5429
ApoI r'AATT_y
Cuts at: 780 1092 1580 2433 4782 5038 780
Size: 312 488 853 2349 256 1171
Fragments arranged by size:
2349 1171 853 488 312 256
AseI AT'TA_AT
Cuts at: 20 1789 20
Size: 1769 3660
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5429
AvaI C'yCGr_G
Cuts at: 265 2449 265
Size: 2184 3245
AvrII C'CTAG_G
Cuts at: 343 343
Size: 5429
BamHI G'GATC_C
Cuts at: 235 2454 235
Size: 2219 3210
BanI G'GyrC_C
Cuts at: 2445 2859 3107 3142 2445
Size: 414 248 35 4732
Fragments arranged by size:
4732 414 248 35
BanII G_rGCy'C
Cuts at: 1281 2443 3473 1281
Size: 1162 1030 3237
Fragments arranged by size:
3237 1162 1030
BbeI G_GCGC'C
Cuts at: 2863 3111 2863
Size: 248 5181
BbsI GAAGACnn'nnnn_
Cuts at: 200 200
Size: 5429
BciVI GTATCCnnnnn_n'
Cuts at: 1251 1430 1707 3327 4088 5429 1251
Size: 179 277 1620 761 1341 1251
Fragments arranged by size:
1620 1341 1251 761 277 179
BclI T'GATC_A
Cuts at: 2949 2949
Size: 5429
BfrBI ATG'CAT
Cuts at: 1639 1639
Size: 5429
BglII A'GATC_T
Cuts at: 707 2078 2944 4981 707
Size: 1371 866 2037 1155
Fragments arranged by size:
2037 1371 1155 866
BlpI GC'TnA_GC
Cuts at: 641 2541 641
Size: 1900 3529
Bme1580I G_kGCm'C
Cuts at: 3054 3147 4203 3054
Size: 93 1056 4280
Fragments arranged by size:
4280 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 726 1003 2641 2722 3057 5201 726
Size: 277 1638 81 335 2144 954
Fragments arranged by size:
2144 1638 954 335 277 81
BmtI G_CTAG'C
Cuts at: 571 571
Size: 5429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 634 3796 634
Size: 3162 2267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 1408 1623 2393 191
Size: 1217 215 770 3227
Fragments arranged by size:
3227 1217 770 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 1008 2605 3484 3976 4274 261
Size: 747 1597 879 492 298 1416
Fragments arranged by size:
1597 1416 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5133 5133
Size: 5429
BsaAI yAC'GTr
Cuts at: 1569 2782 1569
Size: 1213 4216
BsaBI GATnn'nnATC
Cuts at: 6 712 2943 4980 6
Size: 706 2231 2037 455
Fragments arranged by size:
2231 2037 706 455
BsaHI Gr'CG_yC
Cuts at: 2401 2860 3108 3810 5306 2401
Size: 459 248 702 1496 2524
Fragments arranged by size:
2524 1496 702 459 248
BsaWI w'CCGG_w
Cuts at: 1652 2043 2618 3139 3817 4091 4238 4649 1652
Size: 391 575 521 678 274 147 411 2432
Fragments arranged by size:
2432 678 575 521 411 391 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 164 178 194 520 550 148
Size: 16 14 16 326 30 5027
Fragments arranged by size:
5027 326 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 1532 544
Size: 988 4441
BseYI C'CCAG_C
Cuts at: 379 1266 2854 3612 4189 4685 379
Size: 887 1588 758 577 496 1123
Fragments arranged by size:
1588 1123 887 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 746 2233 246
Size: 500 1487 3442
Fragments arranged by size:
3442 1487 500
BsiEI CG_ry'CG
Cuts at: 104 2971 3017 4225 104
Size: 2867 46 1208 1308
Fragments arranged by size:
2867 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2443 3221 3411 4203 2443
Size: 778 190 792 3669
Fragments arranged by size:
3669 792 778 190
BsmI GAATG_Cn'
Cuts at: 684 1839 684
Size: 1155 4274
BsmBI CGTCTCn'nnnn_
Cuts at: 837 837
Size: 5429
Bsp1286I G_dGCh'C
Cuts at: 1281 2443 3054 3147 3221 3411 3473 4203 1281
Size: 1162 611 93 74 190 62 730 2507
Fragments arranged by size:
2507 1162 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4649 4649
Size: 5429
BspHI T'CATG_A
Cuts at: 4605 4605
Size: 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2479 2693 2995 3376 2479
Size: 214 302 381 4532
Fragments arranged by size:
4532 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3721 3775 5259 5424 50
Size: 3671 54 1484 165 55
Fragments arranged by size:
3671 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3341 3341
Size: 5429
BsrFI r'CCGG_y
Cuts at: 3427 3608 3817 3427
Size: 181 209 5039
Fragments arranged by size:
5039 209 181
BsrGI T'GTAC_A
Cuts at: 1729 1729
Size: 5429
BssHII G'CGCG_C
Cuts at: 389 389
Size: 5429
BssSI C'ACGA_G
Cuts at: 3700 4058 3700
Size: 358 5071
BstAPI GCAn_nnn'nTGC
Cuts at: 870 2806 870
Size: 1936 3493
BstBI TT'CG_AA
Cuts at: 1957 3790 1957
Size: 1833 3596
BstYI r'GATC_y
Cuts at: 1 235 667 707 1373 2078 2454 2921 2944
Size: 234 432 40 666 705 376 467 23
Cuts at: 2944 3279 3525 4537 4623 4635 4981 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 705 666 467 449 432 376 346
335 246 234 86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 1272 1272
Size: 5429
BtgI C'CryG_G
Cuts at: 1154 1154
Size: 5429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3379 3560 3723 3379
Size: 181 163 5085
Fragments arranged by size:
5085 181 163
BtsI GCAGTG_nn'
Cuts at: 513 2672 513
Size: 2159 3270
DraI TTT'AAA
Cuts at: 1464 1841 2428 1464
Size: 377 587 4465
Fragments arranged by size:
4465 587 377
DraIII CAC_nnn'GTG
Cuts at: 2017 2017
Size: 5429
DrdI GACnn_nn'nnGTC
Cuts at: 3135 3993 3135
Size: 858 4571
EaeI y'GGCC_r
Cuts at: 2151 3014 3188 3579 3606 4951 2151
Size: 863 174 391 27 1345 2629
Fragments arranged by size:
2629 1345 863 391 174 27
EagI C'GGCC_G
Cuts at: 3014 3014
Size: 5429
EarI CTCTTCn'nnn_
Cuts at: 1100 1239 3452 3662 1100
Size: 139 2213 210 2867
Fragments arranged by size:
2867 2213 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3637 3947 4093 5239 3637
Size: 310 146 1146 3827
Fragments arranged by size:
3827 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2441 2441
Size: 5429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 1408 1623 2228 2264 2393 3254 3686 4433
Size: 1217 215 605 36 129 861 432 747
Cuts at: 4433 4701 191
Size: 268 919
Fragments arranged by size:
1217 919 861 747 605 432 268 215
129 36
EcoO109I rG'GnC_Cy
Cuts at: 1164 2568 1164
Size: 1404 4025
EcoRI G'AATT_C
Cuts at: 780 2433 780
Size: 1653 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 3448 2162
Size: 1286 4143
FspI TGC'GCA
Cuts at: 3210 3210
Size: 5429
HaeII r_GCGC'y
Cuts at: 2863 3111 4133 5112 2863
Size: 248 1022 979 3180
Fragments arranged by size:
3180 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 1705 1737 1795 1827 1993 2025 2086
Size: 32 1153 32 58 32 166 32 61
Cuts at: 2086 2106 2118 2138 3271 3301 3303 3333 520
Size: 20 12 20 1133 30 2 30 2616
Fragments arranged by size:
2616 1153 1133 166 61 58 32 32
32 32 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1298 2468 1298
Size: 1170 4259
HpaI GTT'AAC
Cuts at: 1298 1298
Size: 5429
KasI G'GCGC_C
Cuts at: 2859 3107 2859
Size: 248 5181
KpnI G_GTAC'C
Cuts at: 2449 2449
Size: 5429
MluI A'CGCG_T
Cuts at: 4862 4862
Size: 5429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 236 761 1038 1498 1775 4100 4284 213
Size: 23 525 277 460 277 2325 184 1358
Fragments arranged by size:
2325 1358 525 460 277 277 184 23
MscI TGG'CCA
Cuts at: 2153 3190 2153
Size: 1037 4392
MslI CAynn'nnrTG
Cuts at: 559 1634 1819 2046 3418 3545 559
Size: 1075 185 227 1372 127 2443
Fragments arranged by size:
2443 1372 1075 227 185 127
MspA1I CmG'CkG
Cuts at: 597 1194 2310 2540 2854 3214 4227 4472 597
Size: 597 1116 230 314 360 1013 245 1554
Fragments arranged by size:
1554 1116 1013 597 360 314 245 230
NaeI GCC'GGC
Cuts at: 3610 3610
Size: 5429
NarI GG'CG_CC
Cuts at: 2860 3108 2860
Size: 248 5181
NcoI C'CATG_G
Cuts at: 1154 1154
Size: 5429
NdeI CA'TA_TG
Cuts at: 315 315
Size: 5429
NgoMIV G'CCGG_C
Cuts at: 3608 3608
Size: 5429
NheI G'CTAG_C
Cuts at: 567 567
Size: 5429
NsiI A_TGCA'T
Cuts at: 1641 1641
Size: 5429
NspI r_CATG'y
Cuts at: 978 1639 1643 2482 2982 3889 978
Size: 661 4 839 500 907 2518
Fragments arranged by size:
2518 907 839 661 500 4
PciI A'CATG_T
Cuts at: 3885 3885
Size: 5429
PflMI CCAn_nnn'nTGG
Cuts at: 2310 2310
Size: 5429
PmeI GTTT'AAAC
Cuts at: 2428 2428
Size: 5429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 473 505 4568 4600 473
Size: 32 4063 32 1302
Fragments arranged by size:
4063 1302 32 32
PstI C_TGCA'G
Cuts at: 694 2476 3161 694
Size: 1782 685 2962
Fragments arranged by size:
2962 1782 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5429
PvuII CAG'CTG
Cuts at: 597 2310 2854 3214 597
Size: 1713 544 360 2812
Fragments arranged by size:
2812 1713 544 360
RsrII CG'GwC_CG
Cuts at: 3624 3624
Size: 5429
SacI G_AGCT'C
Cuts at: 2443 2443
Size: 5429
SalI G'TCGA_C
Cuts at: 2466 2466
Size: 5429
SapI GCTCTTCn'nnn_
Cuts at: 3452 3662 3452
Size: 210 5219
SbfI CC_TGCA'GG
Cuts at: 2476 2476
Size: 5429
ScaI AGT'ACT
Cuts at: 1846 4946 1846
Size: 3100 2329
SexAI A'CCwGG_T
Cuts at: 1890 1890
Size: 5429
SfcI C'TryA_G
Cuts at: 33 690 2472 3157 4150 4341 33
Size: 657 1782 685 993 191 1121
Fragments arranged by size:
1782 1121 993 685 657 191
SfoI GGC'GCC
Cuts at: 2861 3109 2861
Size: 248 5181
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5429
SmaI CCC'GGG
Cuts at: 2451 2451
Size: 5429
SmlI C'TyrA_G
Cuts at: 240 987 2584 3499 3991 4253 240
Size: 747 1597 915 492 262 1416
Fragments arranged by size:
1597 1416 915 747 492 262
SnaBI TAC'GTA
Cuts at: 1569 1569
Size: 5429
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5429
SphI G_CATG'C
Cuts at: 978 1639 2482 2982 978
Size: 661 843 500 3425
Fragments arranged by size:
3425 843 661 500
SspI AAT'ATT
Cuts at: 1899 1899
Size: 5429
StyI C'CwwG_G
Cuts at: 327 343 1154 2563 2917 327
Size: 16 811 1409 354 2839
Fragments arranged by size:
2839 1409 811 354 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2098 3012 3145 3604 3813 2098
Size: 914 133 459 209 3714
Fragments arranged by size:
3714 914 459 209 133
TatI w'GTAC_w
Cuts at: 1729 1844 4944 1729
Size: 115 3100 2214
Fragments arranged by size:
3100 2214 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4946 4978 4946
Size: 32 5397
Tth111I GACn'n_nGTC
Cuts at: 732 3226 732
Size: 2494 2935
XbaI T'CTAG_A
Cuts at: 2460 2460
Size: 5429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2924 2924
Size: 5429
XmaI C'CCGG_G
Cuts at: 2449 2449
Size: 5429
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BglI BmgBI Bpu10I BsiWI
BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII MfeI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI StuI SwaI XhoI XmnI
ZraI