Restriction map for
pF1KB9123
(Circular) (Six-base) MAPSORT of: pF1KB9123.seq Check: 8186 from: 1 to: 4628
pF1KB9123 4628 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1497 1497
Size: 4628
AccI GT'mk_AC
Cuts at: 528 1474 1666 528
Size: 946 192 3490
Fragments arranged by size:
3490 946 192
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1157 1484 1544 2453 2885 3632 3900 218
Size: 939 327 60 909 432 747 268 946
Fragments arranged by size:
946 939 909 747 432 327 268 60
AflIII A'CryG_T
Cuts at: 747 1980 3084 4061 747
Size: 1233 1104 977 1314
Fragments arranged by size:
1314 1233 1104 977
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AleI CACnn'nnGTG
Cuts at: 154 154
Size: 4628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 269 269
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 1933 3500 1933
Size: 1567 3061
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 493 1632 3981 4237 493
Size: 1139 2349 256 884
Fragments arranged by size:
2349 1139 884 256
AseI AT'TA_AT
Cuts at: 20 1519 20
Size: 1499 3129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1648 1648
Size: 4628
BamHI G'GATC_C
Cuts at: 759 792 1653 759
Size: 33 861 3734
Fragments arranged by size:
3734 861 33
BanI G'GyrC_C
Cuts at: 1221 1644 2058 2306 2341 1221
Size: 423 414 248 35 3508
Fragments arranged by size:
3508 423 414 248 35
BanII G_rGCy'C
Cuts at: 115 1059 1642 2672 115
Size: 944 583 1030 2071
Fragments arranged by size:
2071 1030 944 583
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 224 1625 224
Size: 1401 3227
BbvCI CC'TCA_GC
Cuts at: 1164 1164
Size: 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1366 1400 1366
Size: 34 4594
BciVI GTATCCnnnnn_n'
Cuts at: 1450 2526 3287 4628 1450
Size: 1076 761 1341 1450
Fragments arranged by size:
1450 1341 1076 761
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglII A'GATC_T
Cuts at: 328 2143 4180 328
Size: 1815 2037 776
Fragments arranged by size:
2037 1815 776
BlpI GC'TnA_GC
Cuts at: 1358 1740 1358
Size: 382 4246
Bme1580I G_kGCm'C
Cuts at: 2253 2346 3402 2253
Size: 93 1056 3479
Fragments arranged by size:
3479 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1840 1921 2256 4400 1840
Size: 81 335 2144 2068
Fragments arranged by size:
2144 2068 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1133 461
Size: 672 3956
Bpu10I CC'TnA_GC
Cuts at: 1164 1164
Size: 4628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2683 3175 3473 1804
Size: 879 492 298 2959
Fragments arranged by size:
2959 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3290 3437 3848 1817
Size: 521 678 274 147 411 2597
Fragments arranged by size:
2597 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 175 205 637 667 175
Size: 30 432 30 4136
Fragments arranged by size:
4136 432 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 962 965 968 995 998 1001 1025 1130 1152
Size: 3 3 27 3 3 24 105 22
Cuts at: 1152 1241 962
Size: 89 4349
Fragments arranged by size:
4349 105 89 27 24 22 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2053 2811 3388 3884 2053
Size: 758 577 496 2797
Fragments arranged by size:
2797 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 309 1052 1475 265
Size: 44 743 423 3418
Fragments arranged by size:
3418 743 423 44
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 1059 1359 1642 2420 2610 3402 115
Size: 944 300 283 778 190 792 1341
Fragments arranged by size:
1341 944 792 778 300 283 190
Bsp1286I G_dGCh'C
Cuts at: 115 1059 1359 1642 2253 2346 2420 2610 2672
Size: 944 300 283 611 93 74 190 62
Cuts at: 2672 3402 115
Size: 730 1341
Fragments arranged by size:
1341 944 730 611 300 283 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1497 1678 1892 2194 2575 1497
Size: 181 214 302 381 3550
Fragments arranged by size:
3550 381 302 214 181
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 2626 2807 3016 2626
Size: 181 209 4238
Fragments arranged by size:
4238 209 181
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 2005 2005
Size: 4628
BstBI TT'CG_AA
Cuts at: 498 2989 498
Size: 2491 2137
BstEII G'GTnAC_C
Cuts at: 147 147
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 742 742
Size: 4628
BstYI r'GATC_y
Cuts at: 1 328 555 759 792 1653 2120 2143 2478
Size: 327 227 204 33 861 467 23 335
Cuts at: 2478 2724 3736 3822 3834 4180 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 861 467 449 346 335 327 246
227 204 86 33 23 12
BtgI C'CryG_G
Cuts at: 139 581 139
Size: 442 4186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 245 297 1871 245
Size: 52 1574 3002
Fragments arranged by size:
3002 1574 52
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 1387 1464 2213 2387 2778 2805 4150 1387
Size: 77 749 174 391 27 1345 1865
Fragments arranged by size:
1865 1345 749 391 174 77 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 202 688 1020 2651 2861 202
Size: 486 332 1631 210 1969
Fragments arranged by size:
1969 1631 486 332 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2836 3146 3292 4438 2836
Size: 310 146 1146 3026
Fragments arranged by size:
3026 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 1057 1640 113
Size: 944 583 3101
Fragments arranged by size:
3101 944 583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 461 1133 1157 1484 1544 2453 2885 3632
Size: 243 672 24 327 60 909 432 747
Cuts at: 3632 3900 218
Size: 268 946
Fragments arranged by size:
946 909 747 672 432 327 268 243
60 24
EcoO109I rG'GnC_Cy
Cuts at: 161 1000 1177 1767 161
Size: 839 177 590 3022
Fragments arranged by size:
3022 839 590 177
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 478 1348 2647 478
Size: 870 1299 2459
Fragments arranged by size:
2459 1299 870
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 1034 2062 2310 3332 4311 1034
Size: 1028 248 1022 979 1351
Fragments arranged by size:
1351 1028 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 1122 1154 1424 1456 2470 2500 2502
Size: 32 915 32 270 32 1014 30 2
Cuts at: 2502 2532 175
Size: 30 2271
Fragments arranged by size:
2271 1014 915 270 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 529 645 1667 529
Size: 116 1022 3490
Fragments arranged by size:
3490 1022 116
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1467 3299 3483 1330
Size: 137 1832 184 2475
Fragments arranged by size:
2475 1832 184 137
MscI TGG'CCA
Cuts at: 1466 2389 1466
Size: 923 3705
MslI CAynn'nnrTG
Cuts at: 154 2617 2744 154
Size: 2463 127 2038
Fragments arranged by size:
2463 2038 127
MspA1I CmG'CkG
Cuts at: 141 1066 1739 2053 2413 3426 3671 141
Size: 925 673 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 925 673 360 314 245
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 581 581
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 751 1681 2181 3088 751
Size: 930 500 907 2291
Fragments arranged by size:
2291 930 907 500
PciI A'CATG_T
Cuts at: 747 3084 747
Size: 2337 2291
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 161 1000 161
Size: 839 3789
PstI C_TGCA'G
Cuts at: 1317 1675 2360 1317
Size: 358 685 3585
Fragments arranged by size:
3585 685 358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 1066 2053 2413 1066
Size: 987 360 3281
Fragments arranged by size:
3281 987 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 115 1059 1642 115
Size: 944 583 3101
Fragments arranged by size:
3101 944 583
SacII CC_GC'GG
Cuts at: 142 142
Size: 4628
SalI G'TCGA_C
Cuts at: 527 1665 527
Size: 1138 3490
SanDI GG'GwC_CC
Cuts at: 161 161
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SfcI C'TryA_G
Cuts at: 33 587 1313 1671 2356 3349 3540 33
Size: 554 726 358 685 993 191 1121
Fragments arranged by size:
1121 993 726 685 554 358 191
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 1783 2698 3190 3452 1783
Size: 915 492 262 2959
Fragments arranged by size:
2959 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
SspI AAT'ATT
Cuts at: 347 347
Size: 4628
StyI C'CwwG_G
Cuts at: 581 704 1762 2116 581
Size: 123 1058 354 3093
Fragments arranged by size:
3093 1058 354 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1501 1509 2211 2344 2803 3012 1501
Size: 8 702 133 459 209 3117
Fragments arranged by size:
3117 702 459 209 133 8
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4596
Tth111I GACn'n_nGTC
Cuts at: 228 2425 228
Size: 2197 2431
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 4628
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 811 811
Size: 4628
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI