Restriction map for
pF1KB9131
(Circular) (Six-base) MAPSORT of: pF1KB9131.seq Check: 6614 from: 1 to: 4850
pF1KB9131 4850 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1888 1888
Size: 4850
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 2675 3107 3460 4122 550
Size: 2125 432 353 662 1278
Fragments arranged by size:
2125 1278 662 432 353
AflIII A'CryG_T
Cuts at: 2202 4002 4283 2202
Size: 1800 281 2769
Fragments arranged by size:
2769 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 371 371
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 2155 3593 2155
Size: 1438 3412
ApaLI G'TGCA_C
Cuts at: 353 1709 3688 353
Size: 1356 1979 1515
Fragments arranged by size:
1979 1515 1356
ApoI r'AATT_y
Cuts at: 391 1318 1360 1570 1854 4203 4459 391
Size: 927 42 210 284 2349 256 782
Fragments arranged by size:
2349 927 782 284 256 210 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 1870 1870
Size: 4850
BamHI G'GATC_C
Cuts at: 1284 1875 1284
Size: 591 4259
BanI G'GyrC_C
Cuts at: 1866 2280 2528 2563 1866
Size: 414 248 35 4153
Fragments arranged by size:
4153 414 248 35
BanII G_rGCy'C
Cuts at: 888 1823 1864 2894 888
Size: 935 41 1030 2844
Fragments arranged by size:
2844 1030 935 41
BbeI G_GCGC'C
Cuts at: 2284 2532 2284
Size: 248 4602
BbsI GAAGACnn'nnnn_
Cuts at: 700 700
Size: 4850
BciVI GTATCCnnnnn_n'
Cuts at: 888 2748 3804 4850 888
Size: 1860 1056 1046 888
Fragments arranged by size:
1860 1056 1046 888
BclI T'GATC_A
Cuts at: 1002 2370 1002
Size: 1368 3482
BfrBI ATG'CAT
Cuts at: 1092 1248 1092
Size: 156 4694
BglII A'GATC_T
Cuts at: 1389 1785 2365 4402 1389
Size: 396 580 2037 1837
Fragments arranged by size:
2037 1837 580 396
BlpI GC'TnA_GC
Cuts at: 156 1021 1962 156
Size: 865 941 3044
Fragments arranged by size:
3044 941 865
Bme1580I G_kGCm'C
Cuts at: 357 1713 2475 2568 3692 357
Size: 1356 762 93 1124 1515
Fragments arranged by size:
1515 1356 1124 762 93
BmrI ACTGGGnnnn_n'
Cuts at: 2062 2143 2478 4622 2062
Size: 81 335 2144 2290
Fragments arranged by size:
2290 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1289 1352 3217 1289
Size: 63 1865 2922
Fragments arranged by size:
2922 1865 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1256 1322 826
Size: 430 66 4354
Fragments arranged by size:
4354 430 66
Bpu10I CC'TnA_GC
Cuts at: 647 647
Size: 4850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 2026 2905 3619 3917 147
Size: 1879 879 714 298 1080
Fragments arranged by size:
1879 1080 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4554 4554
Size: 4850
BsaAI yAC'GTr
Cuts at: 2203 2203
Size: 4850
BsaBI GATnn'nnATC
Cuts at: 6 2364 4401 6
Size: 2358 2037 455
Fragments arranged by size:
2358 2037 455
BsaHI Gr'CG_yC
Cuts at: 436 2281 2529 3231 4727 436
Size: 1845 248 702 1496 559
Fragments arranged by size:
1845 1496 702 559 248
BsaWI w'CCGG_w
Cuts at: 2039 2560 3238 3649 3796 4070 2039
Size: 521 678 411 147 274 2819
Fragments arranged by size:
2819 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 207 824 971 1831 204
Size: 3 617 147 860 3223
Fragments arranged by size:
3223 860 617 147 3
BseYI C'CCAG_C
Cuts at: 1426 2275 3033 3698 4106 1426
Size: 849 758 665 408 2170
Fragments arranged by size:
2170 849 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2392 2438 3668 104
Size: 2288 46 1230 1286
Fragments arranged by size:
2288 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 357 888 1713 1823 1864 2642 2832 3692 357
Size: 531 825 110 41 778 190 860 1515
Fragments arranged by size:
1515 860 825 778 531 190 110 41
BsmI GAATG_Cn'
Cuts at: 123 1250 123
Size: 1127 3723
Bsp1286I G_dGCh'C
Cuts at: 357 888 1713 1823 1864 2475 2568 2642 2832
Size: 531 825 110 41 611 93 74 190
Cuts at: 2832 2894 3692 357
Size: 62 798 1515
Fragments arranged by size:
1515 825 798 611 531 190 110 93
74 62 41
BspHI T'CATG_A
Cuts at: 753 999 3282 753
Size: 246 2283 2321
Fragments arranged by size:
2321 2283 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1900 2114 2416 2797 1900
Size: 214 302 381 3953
Fragments arranged by size:
3953 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3142 3196 4680 4845 50
Size: 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 586 695 1005 1020 2762 586
Size: 109 310 15 1742 2674
Fragments arranged by size:
2674 1742 310 109 15
BsrFI r'CCGG_y
Cuts at: 2848 3029 2848
Size: 181 4669
BssSI C'ACGA_G
Cuts at: 3121 3829 3121
Size: 708 4142
BstAPI GCAn_nnn'nTGC
Cuts at: 2227 2227
Size: 4850
BstBI TT'CG_AA
Cuts at: 3211 3211
Size: 4850
BstEII G'GTnAC_C
Cuts at: 876 876
Size: 4850
BstXI CCAn_nnnn'nTGG
Cuts at: 718 978 718
Size: 260 4590
BstYI r'GATC_y
Cuts at: 1 1284 1389 1548 1785 1875 2342 2365 2700
Size: 1283 105 159 237 90 467 23 335
Cuts at: 2700 2946 3252 3264 3350 4402 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1283 1052 467 449 335 306 246 237
159 105 90 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4850
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2800 2981 3144 2800
Size: 181 163 4506
Fragments arranged by size:
4506 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 2093 1349
Size: 744 4106
ClaI AT'CG_AT
Cuts at: 1653 1653
Size: 4850
DraI TTT'AAA
Cuts at: 995 1421 1799 1833 1849 995
Size: 426 378 34 16 3996
Fragments arranged by size:
3996 426 378 34 16
DrdI GACnn_nn'nnGTC
Cuts at: 2556 3900 2556
Size: 1344 3506
EaeI y'GGCC_r
Cuts at: 2435 2609 3000 3027 4372 2435
Size: 174 391 27 1345 2913
Fragments arranged by size:
2913 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2435 2435
Size: 4850
EarI CTCTTCn'nnn_
Cuts at: 225 834 1604 2873 3083 225
Size: 609 770 1269 210 1992
Fragments arranged by size:
1992 1269 770 609 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3058 3800 3946 4660 3058
Size: 742 146 714 3248
Fragments arranged by size:
3248 742 714 146
EcoICRI GAG'CTC
Cuts at: 886 1821 1862 886
Size: 935 41 3874
Fragments arranged by size:
3874 935 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 826 1256 1322 2675 3107 3460 4122 550
Size: 276 430 66 1353 432 353 662 1278
Fragments arranged by size:
1353 1278 662 432 430 353 276 66
EcoNI CCTnn'n_nnAGG
Cuts at: 1420 1420
Size: 4850
EcoO109I rG'GnC_Cy
Cuts at: 1718 1989 1718
Size: 271 4579
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 501 915 2869 501
Size: 414 1954 2482
Fragments arranged by size:
2482 1954 414
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 2284 2532 3762 4533 2284
Size: 248 1230 771 2601
Fragments arranged by size:
2601 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 421 453 870 902 1292 1324 1516
Size: 32 32 32 417 32 390 32 192
Cuts at: 1516 1548 1777 1809 2692 2722 2724 2754 357
Size: 32 229 32 883 30 2 30 2453
Fragments arranged by size:
2453 883 417 390 229 192 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1413 1674 1889 1413
Size: 261 215 4374
Fragments arranged by size:
4374 261 215
KasI G'GCGC_C
Cuts at: 2280 2528 2280
Size: 248 4602
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 4283 4283
Size: 4850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 588 1620 3609 3793 588
Size: 1032 1989 184 1645
Fragments arranged by size:
1989 1645 1032 184
MscI TGG'CCA
Cuts at: 2611 2611
Size: 4850
MslI CAynn'nnrTG
Cuts at: 112 380 1061 1742 2839 2966 112
Size: 268 681 681 1097 127 1996
Fragments arranged by size:
1996 1097 681 681 268 127
MspA1I CmG'CkG
Cuts at: 786 934 1630 1961 2275 2635 3419 3664 786
Size: 148 696 331 314 360 784 245 1972
Fragments arranged by size:
1972 784 696 360 331 314 245 148
NaeI GCC'GGC
Cuts at: 3031 3031
Size: 4850
NarI GG'CG_CC
Cuts at: 2281 2529 2281
Size: 248 4602
NcoI C'CATG_G
Cuts at: 107 107
Size: 4850
NdeI CA'TA_TG
Cuts at: 1278 1278
Size: 4850
NgoMIV G'CCGG_C
Cuts at: 3029 3029
Size: 4850
NsiI A_TGCA'T
Cuts at: 1094 1250 1094
Size: 156 4694
NspI r_CATG'y
Cuts at: 945 1809 1903 2403 4006 945
Size: 864 94 500 1603 1789
Fragments arranged by size:
1789 1603 864 500 94
PciI A'CATG_T
Cuts at: 4002 4002
Size: 4850
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 611 643 3296 3328 611
Size: 32 2653 32 2133
Fragments arranged by size:
2653 2133 32 32
PpuMI rG'GwC_Cy
Cuts at: 1718 1718
Size: 4850
PstI C_TGCA'G
Cuts at: 1650 1897 2582 1650
Size: 247 685 3918
Fragments arranged by size:
3918 685 247
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 786 934 1630 2275 2635 786
Size: 148 696 645 360 3001
Fragments arranged by size:
3001 696 645 360 148
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 888 1823 1864 888
Size: 935 41 3874
Fragments arranged by size:
3874 935 41
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 834 2873 3083 834
Size: 2039 210 2601
Fragments arranged by size:
2601 2039 210
SbfI CC_TGCA'GG
Cuts at: 1897 1897
Size: 4850
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SexAI A'CCwGG_T
Cuts at: 1406 1406
Size: 4850
SfcI C'TryA_G
Cuts at: 33 1038 1646 1893 2578 3546 3737 33
Size: 1005 608 247 685 968 191 1146
Fragments arranged by size:
1146 1005 968 685 608 247 191
SfoI GGC'GCC
Cuts at: 2282 2530 2282
Size: 248 4602
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 162 2005 2920 3634 3896 162
Size: 1843 915 714 262 1116
Fragments arranged by size:
1843 1116 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4850
SphI G_CATG'C
Cuts at: 945 1903 2403 945
Size: 958 500 3392
Fragments arranged by size:
3392 958 500
SspI AAT'ATT
Cuts at: 294 294
Size: 4850
StuI AGG'CCT
Cuts at: 1750 1750
Size: 4850
StyI C'CwwG_G
Cuts at: 107 1984 2338 107
Size: 1877 354 2619
Fragments arranged by size:
2619 1877 354
SwaI ATTT'AAAT
Cuts at: 995 995
Size: 4850
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2433 2566 3025 3234 2433
Size: 133 459 209 4049
Fragments arranged by size:
4049 459 209 133
TatI w'GTAC_w
Cuts at: 515 4365 515
Size: 3850 1000
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4367 4399 4367
Size: 32 4818
Tth111I GACn'n_nGTC
Cuts at: 1408 2647 1408
Size: 1239 3611
XbaI T'CTAG_A
Cuts at: 1881 1881
Size: 4850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 801 2345 801
Size: 1544 3306
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
XmnI GAAnn'nnTTC
Cuts at: 202 965 202
Size: 763 4087
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI XhoI ZraI