Restriction map for
pF1KB9166
(Circular) (Six-base) MAPSORT of: pF1KB9166.seq Check: 4253 from: 1 to: 9122
pF1KB9166 9122 bp
With 182 enzymes: SgfI *
October 29, 2012 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 235 859 1012 1108 1222 1399 2113 2263 2377
Size: 624 153 96 114 177 714 150 114
Cuts at: 2377 2947 3364 235
Size: 570 417 5993
Fragments arranged by size:
5993 714 624 570 417 177 153 150
114 114 96
AatII G_ACGT'C
Cuts at: 2798 5525 2798
Size: 2727 6395
AccI GT'mk_AC
Cuts at: 2831 4527 5759 6160 2831
Size: 1696 1232 401 5793
Fragments arranged by size:
5793 1696 1232 401
Acc65I G'GTAC_C
Cuts at: 2446 6138 2446
Size: 3692 5430
AclI AA'CG_TT
Cuts at: 8962 8962
Size: 9122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3833 3986 4169 4669 4748 6947 7379 8126 8394
Size: 153 183 500 79 2199 432 747 268
Cuts at: 8394 3833
Size: 4561
Fragments arranged by size:
4561 2199 747 500 432 268 183 153
79
AflIII A'CryG_T
Cuts at: 1289 6474 7578 8555 1289
Size: 5185 1104 977 1856
Fragments arranged by size:
5185 1856 1104 977
AgeI A'CCGG_T
Cuts at: 7510 7510
Size: 9122
AhdI GACnn_n'nnGTC
Cuts at: 2303 2303
Size: 9122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 834 1741 4061 5045 5345 834
Size: 907 2320 984 300 4611
Fragments arranged by size:
4611 2320 984 907 300
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3042 3074 3042
Size: 32 9090
AlwNI CAG_nnn'CTG
Cuts at: 1404 2549 2637 3166 3210 4041 4215 4699 5163
Size: 1145 88 529 44 831 174 484 464
Cuts at: 5163 5463 6427 7994 1404
Size: 300 964 1567 2532
Fragments arranged by size:
2532 1567 1145 964 831 529 484 464
300 174 88 44
ApaI G_GGCC'C
Cuts at: 1388 2393 2506 2699 3372 3571 3611 3992 4143
Size: 1005 113 193 673 199 40 381 151
Cuts at: 4143 5624 1388
Size: 1481 4886
Fragments arranged by size:
4886 1481 1005 673 381 199 193 151
113 40
ApaLI G'TGCA_C
Cuts at: 414 7892 414
Size: 7478 1644
ApoI r'AATT_y
Cuts at: 6126 8475 8731 6126
Size: 2349 256 6517
Fragments arranged by size:
6517 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9122
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9122
AvaI C'yCGr_G
Cuts at: 519 1203 3687 4055 4142 4169 5144 5348 5483
Size: 684 2484 368 87 27 975 204 135
Cuts at: 5483 5808 6142 519
Size: 325 334 3499
Fragments arranged by size:
3499 2484 975 684 368 334 325 204
135 87 27
AvrII C'CTAG_G
Cuts at: 369 826 4491 369
Size: 457 3665 5000
Fragments arranged by size:
5000 3665 457
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2438 2471 2438
Size: 33 9089
BamHI G'GATC_C
Cuts at: 1207 6147 1207
Size: 4940 4182
BanI G'GyrC_C
Cuts at: 223 730 847 1096 1741 2446 2467 6138 6552
Size: 507 117 249 645 705 21 3671 414
Cuts at: 6552 6800 6835 223
Size: 248 35 2510
Fragments arranged by size:
3671 2510 705 645 507 414 249 248
117 35 21
BanII G_rGCy'C
Cuts at: 377 757 1234 1388 2393 2506 2699 3372 3571
Size: 380 477 154 1005 113 193 673 199
Cuts at: 3571 3611 3992 4021 4143 4368 4376 4500 4548
Size: 40 381 29 122 225 8 124 48
Cuts at: 4548 4952 5624 5679 6136 7166 377
Size: 404 672 55 457 1030 2333
Fragments arranged by size:
2333 1030 1005 673 672 477 457 404
381 380 225 199 193 154 124 122
113 55 48 40 29 8
BbeI G_GCGC'C
Cuts at: 6556 6804 6556
Size: 248 8874
BbsI GAAGACnn'nnnn_
Cuts at: 470 1292 5255 470
Size: 822 3963 4337
Fragments arranged by size:
4337 3963 822
BbvCI CC'TCA_GC
Cuts at: 1662 1662
Size: 9122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 655 689 3569 3603 5371 5405 90
Size: 34 531 34 2880 34 1768 34 3807
Fragments arranged by size:
3807 2880 1768 531 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7020 7781 9122 7020
Size: 761 1341 7020
Fragments arranged by size:
7020 1341 761
BclI T'GATC_A
Cuts at: 1530 3888 6642 1530
Size: 2358 2754 4010
Fragments arranged by size:
4010 2754 2358
BglI GCCn_nnn'nGGC
Cuts at: 428 659 728 1616 2264 2354 2609 3095 3461
Size: 231 69 888 648 90 255 486 366
Cuts at: 3461 4495 5239 428
Size: 1034 744 4311
Fragments arranged by size:
4311 1034 888 744 648 486 366 255
231 90 69
BglII A'GATC_T
Cuts at: 6637 8674 6637
Size: 2037 7085
BlpI GC'TnA_GC
Cuts at: 6234 6234
Size: 9122
Bme1580I G_kGCm'C
Cuts at: 270 418 850 976 1099 1203 1388 2085 2120
Size: 148 432 126 123 104 185 697 35
Cuts at: 2120 2211 2393 2506 2655 2699 2979 3372 3571
Size: 91 182 113 149 44 280 393 199
Cuts at: 3571 3611 3908 3992 4143 5005 5624 5660 6747
Size: 40 297 84 151 862 619 36 1087
Cuts at: 6747 6840 7896 270
Size: 93 1056 1496
Fragments arranged by size:
1496 1087 1056 862 697 619 432 393
297 280 199 185 182 151 149 148
126 123 113 104 93 91 84 44
40 36 35
BmgBI CAC'GTC
Cuts at: 290 2148 3126 5286 290
Size: 1858 978 2160 4126
Fragments arranged by size:
4126 2160 1858 978
BmrI ACTGGGnnnn_n'
Cuts at: 2388 2702 2948 3708 4459 5015 5334 5553 6334
Size: 314 246 760 751 556 319 219 781
Cuts at: 6334 6415 6750 8894 2388
Size: 81 335 2144 2616
Fragments arranged by size:
2616 2144 781 760 751 556 335 319
314 246 219 81
BmtI G_CTAG'C
Cuts at: 5512 5512
Size: 9122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 257 891 1563 1677 4248 7489 257
Size: 634 672 114 2571 3241 1890
Fragments arranged by size:
3241 2571 1890 672 634 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1838 2372 2671 2704 3077 3089 3587 3836 3848
Size: 534 299 33 373 12 498 249 12
Cuts at: 3848 4390 4495 5096 5353 1838
Size: 542 105 601 257 5607
Fragments arranged by size:
5607 601 542 534 498 373 299 257
249 105 33 12 12
Bpu10I CC'TnA_GC
Cuts at: 232 1662 3425 4692 4946 232
Size: 1430 1763 1267 254 4408
Fragments arranged by size:
4408 1763 1430 1267 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4624 5089 6298 7177 7669 7967 4624
Size: 465 1209 879 492 298 5779
Fragments arranged by size:
5779 1209 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 1639 3544 3838 4219 4642 4769 5005 5943 8826
Size: 1905 294 381 423 127 236 938 2883
Cuts at: 8826 1639
Size: 1935
Fragments arranged by size:
2883 1935 1905 938 423 381 294 236
127
BsaAI yAC'GTr
Cuts at: 2898 4751 6475 2898
Size: 1853 1724 5545
Fragments arranged by size:
5545 1853 1724
BsaBI GATnn'nnATC
Cuts at: 6 6636 8673 6
Size: 6630 2037 455
Fragments arranged by size:
6630 2037 455
BsaHI Gr'CG_yC
Cuts at: 2795 4524 4530 4605 5413 5522 6553 6801 7503
Size: 1729 6 75 808 109 1031 248 702
Cuts at: 7503 8999 2795
Size: 1496 2918
Fragments arranged by size:
2918 1729 1496 1031 808 702 248 109
75 6
BsaWI w'CCGG_w
Cuts at: 2617 6311 6832 7510 7784 7931 8342 2617
Size: 3694 521 678 274 147 411 3397
Fragments arranged by size:
3694 3397 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1498 1528 2358 2388 3042 3072 4700 4730 5921
Size: 30 830 30 654 30 1628 30 1191
Cuts at: 5921 5951 1498
Size: 30 4669
Fragments arranged by size:
4669 1628 1191 830 654 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1180 1768 2305 4717 4726 4802 5840 6116 1180
Size: 588 537 2412 9 76 1038 276 4186
Fragments arranged by size:
4186 2412 1038 588 537 276 76 9
BseYI C'CCAG_C
Cuts at: 320 379 403 455 1031 1145 1424 1550 2231
Size: 59 24 52 576 114 279 126 681
Cuts at: 2231 2530 2948 3941 4023 4243 5456 5466 5472
Size: 299 418 993 82 220 1213 10 6
Cuts at: 5472 5503 5572 5617 6069 6547 7305 7882 8378
Size: 31 69 45 452 478 758 577 496
Cuts at: 8378 320
Size: 1064
Fragments arranged by size:
1213 1064 993 758 681 577 576 496
478 452 418 299 279 220 126 114
82 69 59 52 45 31 24 10
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1791 2085 2183 2235 2349 2558 2759 3843 5005
Size: 294 98 52 114 209 201 1084 1162
Cuts at: 5005 5305 1791
Size: 300 5608
Fragments arranged by size:
5608 1162 1084 300 294 209 201 114
98 52
BsiEI CG_ry'CG
Cuts at: 104 5847 6664 6710 7918 104
Size: 5743 817 46 1208 1308
Fragments arranged by size:
5743 1308 1208 817 46
BsiHKAI G_wGCw'C
Cuts at: 418 538 1998 4462 4548 4693 6136 6914 7104
Size: 120 1460 2464 86 145 1443 778 190
Cuts at: 7104 7896 418
Size: 792 1644
Fragments arranged by size:
2464 1644 1460 1443 792 778 190 145
120 86
BsmBI CGTCTCn'nnnn_
Cuts at: 280 2802 3116 280
Size: 2522 314 6286
Fragments arranged by size:
6286 2522 314
Bsp1286I G_dGCh'C
Cuts at: 270 377 418 538 757 850 976 1099 1203
Size: 107 41 120 219 93 126 123 104
Cuts at: 1203 1234 1388 1998 2085 2120 2211 2393 2506
Size: 31 154 610 87 35 91 182 113
Cuts at: 2506 2655 2699 2979 3372 3571 3611 3908 3992
Size: 149 44 280 393 199 40 297 84
Cuts at: 3992 4021 4143 4368 4376 4462 4500 4548 4693
Size: 29 122 225 8 86 38 48 145
Cuts at: 4693 4952 5005 5624 5660 5679 6136 6747 6840
Size: 259 53 619 36 19 457 611 93
Cuts at: 6840 6914 7104 7166 7896 270
Size: 74 190 62 730 1496
Fragments arranged by size:
1496 730 619 611 610 457 393 297
280 259 225 219 199 190 182 154
149 145 126 123 122 120 113 107
104 93 93 91 87 86 84 74
62 53 48 44 41 40 38 36
35 31 29 19 8
BspEI T'CCGG_A
Cuts at: 8342 8342
Size: 9122
BspHI T'CATG_A
Cuts at: 8298 8298
Size: 9122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 235 265 859 1012 1108 1222 1399 1706 2113
Size: 30 594 153 96 114 177 307 407
Cuts at: 2113 2230 2263 2377 2575 2947 3364 6172 6386
Size: 117 33 114 198 372 417 2808 214
Cuts at: 6386 6688 7069 235
Size: 302 381 2288
Fragments arranged by size:
2808 2288 594 417 407 381 372 307
302 214 198 177 153 117 114 114
96 33 30
BsrBI CCG'CTC
Cuts at: 50 364 5360 5443 6039 7414 7468 8952 9117
Size: 314 4996 83 596 1375 54 1484 165
Cuts at: 9117 50
Size: 55
Fragments arranged by size:
4996 1484 1375 596 314 165 83 55
54
BsrDI GCAATG_nn'
Cuts at: 5793 7034 5793
Size: 1241 7881
BsrFI r'CCGG_y
Cuts at: 4607 5514 7120 7301 7510 4607
Size: 907 1606 181 209 6219
Fragments arranged by size:
6219 1606 907 209 181
BssHII G'CGCG_C
Cuts at: 5631 5631
Size: 9122
BssSI C'ACGA_G
Cuts at: 5717 7393 7751 5717
Size: 1676 358 7088
Fragments arranged by size:
7088 1676 358
BstAPI GCAn_nnn'nTGC
Cuts at: 737 2206 2885 4429 6499 737
Size: 1469 679 1544 2070 3360
Fragments arranged by size:
3360 2070 1544 1469 679
BstBI TT'CG_AA
Cuts at: 838 7483 838
Size: 6645 2477
BstXI CCAn_nnnn'nTGG
Cuts at: 1022 2239 2943 2956 3561 3737 1022
Size: 1217 704 13 605 176 6407
Fragments arranged by size:
6407 1217 704 605 176 13
BstYI r'GATC_y
Cuts at: 1 638 1207 2128 5547 6147 6614 6637 6972
Size: 637 569 921 3419 600 467 23 335
Cuts at: 6972 7218 8230 8316 8328 8674 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3419 1012 921 637 600 569 467 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2090 2582 4700 4793 2090
Size: 492 2118 93 6419
Fragments arranged by size:
6419 2118 492 93
BtgI C'CryG_G
Cuts at: 1311 2112 2211 2489 3045 3399 3554 4854 4997
Size: 801 99 278 556 354 155 1300 143
Cuts at: 4997 1311
Size: 5436
Fragments arranged by size:
5436 1300 801 556 354 278 155 143
99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3683 4363 7072 7253 7416 3683
Size: 680 2709 181 163 5389
Fragments arranged by size:
5389 2709 680 181 163
BtsI GCAGTG_nn'
Cuts at: 121 571 648 1080 1125 1599 1807 1827 2118
Size: 450 77 432 45 474 208 20 291
Cuts at: 2118 2679 2706 2856 3164 3327 3390 3687 3796
Size: 561 27 150 308 163 63 297 109
Cuts at: 3796 3926 4364 4390 5192 6365 121
Size: 130 438 26 802 1173 2878
Fragments arranged by size:
2878 1173 802 561 474 450 438 432
308 297 291 208 163 150 130 109
77 63 45 27 26 20
ClaI AT'CG_AT
Cuts at: 1763 1763
Size: 9122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1200 1235 1669 1704 1200
Size: 35 434 35 8618
Fragments arranged by size:
8618 434 35 35
DraI TTT'AAA
Cuts at: 6121 6121
Size: 9122
DraIII CAC_nnn'GTG
Cuts at: 799 1131 2206 3448 3805 4998 799
Size: 332 1075 1242 357 1193 4923
Fragments arranged by size:
4923 1242 1193 1075 357 332
DrdI GACnn_nn'nnGTC
Cuts at: 946 6828 7686 946
Size: 5882 858 2382
Fragments arranged by size:
5882 2382 858
EaeI y'GGCC_r
Cuts at: 623 3185 4469 5044 5844 6707 6881 7272 7299
Size: 2562 1284 575 800 863 174 391 27
Cuts at: 7299 8644 623
Size: 1345 1101
Fragments arranged by size:
2562 1345 1284 1101 863 800 575 391
174 27
EagI C'GGCC_G
Cuts at: 5844 6707 5844
Size: 863 8259
EarI CTCTTCn'nnn_
Cuts at: 2805 3781 3809 4505 7145 7355 2805
Size: 976 28 696 2640 210 4572
Fragments arranged by size:
4572 2640 976 696 210 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2515 5533 5741 7330 7640 7786 8932 2515
Size: 3018 208 1589 310 146 1146 2705
Fragments arranged by size:
3018 2705 1589 1146 310 208 146
EcoICRI GAG'CTC
Cuts at: 4546 6134 4546
Size: 1588 7534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1838 2372 2671 2704 3077 3089 3587 3833 3836
Size: 534 299 33 373 12 498 246 3
Cuts at: 3836 3848 3986 4169 4390 4495 4669 4748 5096
Size: 12 138 183 221 105 174 79 348
Cuts at: 5096 5353 6947 7379 8126 8394 1838
Size: 257 1594 432 747 268 2566
Fragments arranged by size:
2566 1594 747 534 498 432 373 348
299 268 257 246 221 183 174 138
105 79 33 12 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2887 3428 4219 4412 5068 2887
Size: 541 791 193 656 6941
Fragments arranged by size:
6941 791 656 541 193
EcoO109I rG'GnC_Cy
Cuts at: 254 708 715 814 1072 1384 1401 2219 2275
Size: 454 7 99 258 312 17 818 56
Cuts at: 2275 2389 2390 2502 2587 2696 3284 3349 3368
Size: 114 1 112 85 109 588 65 19
Cuts at: 3368 3369 3472 3567 3607 3608 3662 3975 3988
Size: 1 103 95 40 1 54 313 13
Cuts at: 3988 3989 4139 4173 4317 4696 4916 4976 5621
Size: 1 150 34 144 379 220 60 645
Cuts at: 5621 5713 5783 6261 254
Size: 92 70 478 3115
Fragments arranged by size:
3115 818 645 588 478 454 379 313
312 258 220 150 144 114 112 109
103 99 95 92 85 70 65 60
56 54 40 34 19 17 13 7
1 1 1 1
EcoRI G'AATT_C
Cuts at: 6126 6126
Size: 9122
EcoRV GAT'ATC
Cuts at: 1761 1761
Size: 9122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 373 3450 7141 373
Size: 3077 3691 2354
Fragments arranged by size:
3691 3077 2354
FspI TGC'GCA
Cuts at: 6903 6903
Size: 9122
HaeII r_GCGC'y
Cuts at: 4789 5515 6556 6804 7826 8805 4789
Size: 726 1041 248 1022 979 5106
Fragments arranged by size:
5106 1041 1022 979 726 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2289 2321 2721 2753 5921 5953 5997 6028 6029
Size: 32 400 32 3168 32 44 31 1
Cuts at: 6029 6060 6964 6994 6996 7026 2289
Size: 31 904 30 2 30 4385
Fragments arranged by size:
4385 3168 904 400 44 32 32 32
31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 693 2832 4528 6161 693
Size: 2139 1696 1633 3654
Fragments arranged by size:
3654 2139 1696 1633
KasI G'GCGC_C
Cuts at: 6552 6800 6552
Size: 248 8874
KpnI G_GTAC'C
Cuts at: 2450 6142 2450
Size: 3692 5430
MluI A'CGCG_T
Cuts at: 8555 8555
Size: 9122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1887 2134 6020 7793 7977 1887
Size: 247 3886 1773 184 3032
Fragments arranged by size:
3886 3032 1773 247 184
MscI TGG'CCA
Cuts at: 625 3187 6883 625
Size: 2562 3696 2864
Fragments arranged by size:
3696 2864 2562
MslI CAynn'nnrTG
Cuts at: 677 1316 2139 2941 3044 4409 7111 7238 677
Size: 639 823 802 103 1365 2702 127 2561
Fragments arranged by size:
2702 2561 1365 823 802 639 127 103
MspA1I CmG'CkG
Cuts at: 344 503 858 1916 2534 2594 2627 2837 3542
Size: 159 355 1058 618 60 33 210 705
Cuts at: 3542 4041 4280 4307 5053 6233 6547 6907 7920
Size: 499 239 27 746 1180 314 360 1013
Cuts at: 7920 8165 344
Size: 245 1301
Fragments arranged by size:
1301 1180 1058 1013 746 705 618 499
360 355 314 245 239 210 159 60
33 27
NaeI GCC'GGC
Cuts at: 4609 5516 7303 4609
Size: 907 1787 6428
Fragments arranged by size:
6428 1787 907
NarI GG'CG_CC
Cuts at: 6553 6801 6553
Size: 248 8874
NcoI C'CATG_G
Cuts at: 1311 2211 2489 3045 1311
Size: 900 278 556 7388
Fragments arranged by size:
7388 900 556 278
NgoMIV G'CCGG_C
Cuts at: 4607 5514 7301 4607
Size: 907 1787 6428
Fragments arranged by size:
6428 1787 907
NheI G'CTAG_C
Cuts at: 5508 5508
Size: 9122
NspI r_CATG'y
Cuts at: 546 1293 5868 6175 6675 7582 546
Size: 747 4575 307 500 907 2086
Fragments arranged by size:
4575 2086 907 747 500 307
PasI CC'CwG_GG
Cuts at: 750 1068 3312 4215 4313 4321 4952 5973 750
Size: 318 2244 903 98 8 631 1021 3899
Fragments arranged by size:
3899 2244 1021 903 631 318 98 8
PciI A'CATG_T
Cuts at: 1289 7578 1289
Size: 6289 2833
PflMI CCAn_nnn'nTGG
Cuts at: 1404 3183 1404
Size: 1779 7343
PfoI T'CCnGG_A
Cuts at: 704 1929 3779 4912 704
Size: 1225 1850 1133 4914
Fragments arranged by size:
4914 1850 1225 1133
PmeI GTTT'AAAC
Cuts at: 6121 6121
Size: 9122
PmlI CAC'GTG
Cuts at: 2898 4751 2898
Size: 1853 7269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3042 3074 8261 8293 3042
Size: 32 5187 32 3871
Fragments arranged by size:
5187 3871 32 32
PpuMI rG'GwC_Cy
Cuts at: 254 708 715 814 1072 1401 2219 2275 2587
Size: 454 7 99 258 329 818 56 312
Cuts at: 2587 3284 3472 3975 4173 4317 4916 254
Size: 697 188 503 198 144 599 4460
Fragments arranged by size:
4460 818 697 599 503 454 329 312
258 198 188 144 99 56 7
PshAI GACnn'nnGTC
Cuts at: 470 1292 1412 1898 3134 6062 470
Size: 822 120 486 1236 2928 3530
Fragments arranged by size:
3530 2928 1236 822 486 120
PspOMI G'GGCC_C
Cuts at: 1384 2389 2502 2695 3368 3567 3607 3988 4139
Size: 1005 113 193 673 199 40 381 151
Cuts at: 4139 5620 1384
Size: 1481 4886
Fragments arranged by size:
4886 1481 1005 673 381 199 193 151
113 40
PstI C_TGCA'G
Cuts at: 1087 3171 3361 3682 3933 3961 4359 5187 5741
Size: 2084 190 321 251 28 398 828 554
Cuts at: 5741 5862 6169 6854 1087
Size: 121 307 685 3355
Fragments arranged by size:
3355 2084 828 685 554 398 321 307
251 190 121 28
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9122
PvuII CAG'CTG
Cuts at: 344 858 1916 2534 2627 3542 4041 6547 6907
Size: 514 1058 618 93 915 499 2506 360
Cuts at: 6907 344
Size: 2559
Fragments arranged by size:
2559 2506 1058 915 618 514 499 360
93
RsrII CG'GwC_CG
Cuts at: 7317 7317
Size: 9122
SacI G_AGCT'C
Cuts at: 4548 6136 4548
Size: 1588 7534
SalI G'TCGA_C
Cuts at: 2830 4526 6159 2830
Size: 1696 1633 5793
Fragments arranged by size:
5793 1696 1633
SanDI GG'GwC_CC
Cuts at: 1072 2587 3975 4173 4317 1072
Size: 1515 1388 198 144 5877
Fragments arranged by size:
5877 1515 1388 198 144
SapI GCTCTTCn'nnn_
Cuts at: 4505 7145 7355 4505
Size: 2640 210 6272
Fragments arranged by size:
6272 2640 210
SbfI CC_TGCA'GG
Cuts at: 6169 6169
Size: 9122
ScaI AGT'ACT
Cuts at: 3880 8639 3880
Size: 4759 4363
SexAI A'CCwGG_T
Cuts at: 5252 5252
Size: 9122
SfcI C'TryA_G
Cuts at: 33 1083 2261 2283 2550 3167 3357 3678 3929
Size: 1050 1178 22 267 617 190 321 251
Cuts at: 3929 3957 4355 5183 5737 5858 6165 6850 7843
Size: 28 398 828 554 121 307 685 993
Cuts at: 7843 8034 33
Size: 191 1121
Fragments arranged by size:
1178 1121 1050 993 828 685 617 554
398 321 307 267 251 191 190 121
28 22
SfoI GGC'GCC
Cuts at: 6554 6802 6554
Size: 248 8874
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9122
SgrAI Cr'CCGG_yG
Cuts at: 5514 5514
Size: 9122
SmaI CCC'GGG
Cuts at: 1205 3689 4057 4144 6144 1205
Size: 2484 368 87 2000 4183
Fragments arranged by size:
4183 2484 2000 368 87
SmlI C'TyrA_G
Cuts at: 4639 5068 6277 7192 7684 7946 4639
Size: 429 1209 915 492 262 5815
Fragments arranged by size:
5815 1209 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9122
SphI G_CATG'C
Cuts at: 546 5868 6175 6675 546
Size: 5322 307 500 2993
Fragments arranged by size:
5322 2993 500 307
SrfI GCCC'GGGC
Cuts at: 4144 4144
Size: 9122
StuI AGG'CCT
Cuts at: 517 2463 2822 2919 3407 3963 517
Size: 1946 359 97 488 556 5676
Fragments arranged by size:
5676 1946 556 488 359 97
StyI C'CwwG_G
Cuts at: 369 826 1311 2211 2489 2784 2874 3045 3335
Size: 457 485 900 278 295 90 171 290
Cuts at: 3335 3632 4491 4923 6096 6256 6610 369
Size: 297 859 432 1173 160 354 2881
Fragments arranged by size:
2881 1173 900 859 485 457 432 354
297 295 290 278 171 160 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 417 1577 3812 4243 4252 5955 6705 6838
Size: 146 1160 2235 431 9 1703 750 133
Cuts at: 6838 7297 7506 271
Size: 459 209 1887
Fragments arranged by size:
2235 1887 1703 1160 750 459 431 209
146 133 9
TatI w'GTAC_w
Cuts at: 3055 3878 4905 8637 3055
Size: 823 1027 3732 3540
Fragments arranged by size:
3732 3540 1027 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1276 1308 2358 2390 3775 3807 3967 3999 4089
Size: 32 1050 32 1385 32 160 32 90
Cuts at: 4089 4121 4285 4317 4700 4732 8639 8671 1276
Size: 32 164 32 383 32 3907 32 1727
Fragments arranged by size:
3907 1727 1385 1050 383 164 160 90
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4587 5643 5697 5751 6919 4587
Size: 1056 54 54 1168 6790
Fragments arranged by size:
6790 1168 1056 54 54
XbaI T'CTAG_A
Cuts at: 6153 6153
Size: 9122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 268 964 1111 1309 1813 2863 3211 5241 6617
Size: 696 147 198 504 1050 348 2030 1376
Cuts at: 6617 268
Size: 2773
Fragments arranged by size:
2773 2030 1376 1050 696 504 348 198
147
XmaI C'CCGG_G
Cuts at: 1203 3687 4055 4142 6142 1203
Size: 2484 368 87 2000 4183
Fragments arranged by size:
4183 2484 2000 368 87
ZraI GAC'GTC
Cuts at: 2796 5523 2796
Size: 2727 6395
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI BfrBI BsiWI BsmI BsrGI
BstEII BstZ17I FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PsiI PspXI PsrI SacII
SfiI SnaBI SspI SwaI XhoI XmnI