Restriction map for
pF1KB9177
(Circular) (Six-base) MAPSORT of: pF1KB9177.seq Check: 8604 from: 1 to: 6527
pF1KB9177 6527 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 494 2142 2431 494
Size: 1648 289 4590
Fragments arranged by size:
4590 1648 289
AccI GT'mk_AC
Cuts at: 1588 3565 1588
Size: 1977 4550
Acc65I G'GTAC_C
Cuts at: 3543 3543
Size: 6527
AclI AA'CG_TT
Cuts at: 6367 6367
Size: 6527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2716 4352 4784 5137 5799 2387
Size: 329 1636 432 353 662 3115
Fragments arranged by size:
3115 1636 662 432 353 329
AfeI AGC'GCT
Cuts at: 220 220
Size: 6527
AflIII A'CryG_T
Cuts at: 546 2013 3464 3879 5679 5960 546
Size: 1467 1451 415 1800 281 1113
Fragments arranged by size:
1800 1467 1451 1113 415 281
AgeI A'CCGG_T
Cuts at: 1091 1091
Size: 6527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2344 2344
Size: 6527
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 622 654 1357 1389 1640 1672 1885 1917 3067
Size: 32 703 32 251 32 213 32 1150
Cuts at: 3067 3099 622
Size: 32 4050
Fragments arranged by size:
4050 1150 703 251 213 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 2388 2474 2976 3015 3832 5270 2388
Size: 86 502 39 817 1438 3645
Fragments arranged by size:
3645 1438 817 502 86 39
ApaI G_GGCC'C
Cuts at: 283 1996 2344 283
Size: 1713 348 4466
Fragments arranged by size:
4466 1713 348
ApaLI G'TGCA_C
Cuts at: 463 550 1132 5365 463
Size: 87 582 4233 1625
Fragments arranged by size:
4233 1625 582 87
ApoI r'AATT_y
Cuts at: 3531 5880 6136 3531
Size: 2349 256 3922
Fragments arranged by size:
3922 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6527
AvaI C'yCGr_G
Cuts at: 328 1822 2281 2514 2584 2933 3110 3547 328
Size: 1494 459 233 70 349 177 437 3308
Fragments arranged by size:
3308 1494 459 437 349 233 177 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 294 327 294
Size: 33 6494
BamHI G'GATC_C
Cuts at: 592 1154 3552 592
Size: 562 2398 3567
Fragments arranged by size:
3567 2398 562
BanI G'GyrC_C
Cuts at: 120 862 1344 1818 1945 3543 3957 4205 4240
Size: 742 482 474 127 1598 414 248 35
Cuts at: 4240 120
Size: 2407
Fragments arranged by size:
2407 1598 742 482 474 414 248 127
35
BanII G_rGCy'C
Cuts at: 268 283 483 1996 2282 2344 2410 2591 3293
Size: 15 200 1513 286 62 66 181 702
Cuts at: 3293 3541 4571 268
Size: 248 1030 2224
Fragments arranged by size:
2224 1513 1030 702 286 248 200 181
66 62 15
BbeI G_GCGC'C
Cuts at: 3961 4209 3961
Size: 248 6279
BbsI GAAGACnn'nnnn_
Cuts at: 405 1330 3142 405
Size: 925 1812 3790
Fragments arranged by size:
3790 1812 925
BbvCI CC'TCA_GC
Cuts at: 2336 3512 2336
Size: 1176 5351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1043 1077 1043
Size: 34 6493
BciVI GTATCCnnnnn_n'
Cuts at: 1642 4425 5481 6527 1642
Size: 2783 1056 1046 1642
Fragments arranged by size:
2783 1642 1056 1046
BclI T'GATC_A
Cuts at: 2678 4047 2678
Size: 1369 5158
BglI GCCn_nnn'nGGC
Cuts at: 716 851 3451 716
Size: 135 2600 3792
Fragments arranged by size:
3792 2600 135
BglII A'GATC_T
Cuts at: 4042 6079 4042
Size: 2037 4490
BlpI GC'TnA_GC
Cuts at: 1669 3639 1669
Size: 1970 4557
Bme1580I G_kGCm'C
Cuts at: 125 283 467 554 793 925 1029 1136 1349
Size: 158 184 87 239 132 104 107 213
Cuts at: 1349 1445 1454 1823 1996 2344 4152 4245 5369
Size: 96 9 369 173 348 1808 93 1124
Cuts at: 5369 125
Size: 1283
Fragments arranged by size:
1808 1283 1124 369 348 239 213 184
173 158 132 107 104 96 93 87
9
BmgBI CAC'GTC
Cuts at: 573 2190 573
Size: 1617 4910
BmrI ACTGGGnnnn_n'
Cuts at: 1601 2024 2338 2596 3739 3820 4155 6299 1601
Size: 423 314 258 1143 81 335 2144 1829
Fragments arranged by size:
2144 1829 1143 423 335 314 258 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1914 2751 4894 1914
Size: 837 2143 3547
Fragments arranged by size:
3547 2143 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 497 1017 1042 1207 2024 2310 316
Size: 181 520 25 165 817 286 4533
Fragments arranged by size:
4533 817 520 286 181 165 25
Bpu10I CC'TnA_GC
Cuts at: 1736 2336 2544 2593 2959 3512 1736
Size: 600 208 49 366 553 4751
Fragments arranged by size:
4751 600 553 366 208 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4582 5296 5594 3703
Size: 879 714 298 4636
Fragments arranged by size:
4636 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1506 1518 2165 2518 6231 1506
Size: 12 647 353 3713 1802
Fragments arranged by size:
3713 1802 647 353 12
BsaAI yAC'GTr
Cuts at: 462 547 2016 3880 462
Size: 85 1469 1864 3109
Fragments arranged by size:
3109 1864 1469 85
BsaBI GATnn'nnATC
Cuts at: 6 4041 6078 6
Size: 4035 2037 455
Fragments arranged by size:
4035 2037 455
BsaHI Gr'CG_yC
Cuts at: 272 410 3147 3958 4206 4908 6404 272
Size: 138 2737 811 248 702 1496 395
Fragments arranged by size:
2737 1496 811 702 395 248 138
BsaWI w'CCGG_w
Cuts at: 1091 1151 3716 4237 4915 5326 5473 5747 1091
Size: 60 2565 521 678 411 147 274 1871
Fragments arranged by size:
2565 1871 678 521 411 274 147 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1454 1484 3067 3097 3107 3137 1454
Size: 30 1583 30 10 30 4844
Fragments arranged by size:
4844 1583 30 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1703 2218 2536 3133 3529 1703
Size: 515 318 597 396 4701
Fragments arranged by size:
4701 597 515 396 318
BseYI C'CCAG_C
Cuts at: 215 433 757 764 2158 2922 3952 4710 5375
Size: 218 324 7 1394 764 1030 758 665
Cuts at: 5375 5783 215
Size: 408 959
Fragments arranged by size:
1394 1030 959 764 758 665 408 324
218 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 682 1244 1445 1537 1684 1965 1987 1994
Size: 155 562 201 92 147 281 22 7
Cuts at: 1994 2170 2186 527
Size: 176 16 4868
Fragments arranged by size:
4868 562 281 201 176 155 147 92
22 16 7
BsiEI CG_ry'CG
Cuts at: 104 309 4069 4115 5345 104
Size: 205 3760 46 1230 1286
Fragments arranged by size:
3760 1286 1230 205 46
BsiHKAI G_wGCw'C
Cuts at: 467 554 1136 1292 3293 3541 4319 4509 5369
Size: 87 582 156 2001 248 778 190 860
Cuts at: 5369 467
Size: 1625
Fragments arranged by size:
2001 1625 860 778 582 248 190 156
87
BsmI GAATG_Cn'
Cuts at: 2270 2270
Size: 6527
BsmBI CGTCTCn'nnnn_
Cuts at: 1195 1902 2481 3154 1195
Size: 707 579 673 4568
Fragments arranged by size:
4568 707 673 579
Bsp1286I G_dGCh'C
Cuts at: 125 268 283 467 483 554 793 925 1029
Size: 143 15 184 16 71 239 132 104
Cuts at: 1029 1136 1292 1349 1445 1454 1823 1996 2282
Size: 107 156 57 96 9 369 173 286
Cuts at: 2282 2344 2410 2591 3293 3541 4152 4245 4319
Size: 62 66 181 702 248 611 93 74
Cuts at: 4319 4509 4571 5369 125
Size: 190 62 798 1283
Fragments arranged by size:
1283 798 702 611 369 286 248 239
190 184 181 173 156 143 132 107
104 96 93 74 71 66 62 62
57 16 15 9
BspHI T'CATG_A
Cuts at: 1718 4959 1718
Size: 3241 3286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 494 2142 2431 2888 3577 3791 4093 4474
Size: 288 1648 289 457 689 214 302 381
Cuts at: 4474 206
Size: 2259
Fragments arranged by size:
2259 1648 689 457 381 302 289 288
214
BsrBI CCG'CTC
Cuts at: 50 677 1884 4819 4873 6357 6522 50
Size: 627 1207 2935 54 1484 165 55
Fragments arranged by size:
2935 1484 1207 627 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4439 4439
Size: 6527
BsrFI r'CCGG_y
Cuts at: 1091 1415 1559 1673 1787 4525 4706 1091
Size: 324 144 114 114 2738 181 2912
Fragments arranged by size:
2912 2738 324 181 144 114 114
BssHII G'CGCG_C
Cuts at: 349 349
Size: 6527
BssSI C'ACGA_G
Cuts at: 403 4798 5506 403
Size: 4395 708 1424
Fragments arranged by size:
4395 1424 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1131 2474 2976 3904 1131
Size: 1343 502 928 3754
Fragments arranged by size:
3754 1343 928 502
BstBI TT'CG_AA
Cuts at: 4888 4888
Size: 6527
BstXI CCAn_nnnn'nTGG
Cuts at: 699 714 1599 699
Size: 15 885 5627
Fragments arranged by size:
5627 885 15
BstYI r'GATC_y
Cuts at: 1 592 988 1154 1163 2084 3552 4019 4042
Size: 591 396 166 9 921 1468 467 23
Cuts at: 4042 4377 4623 4929 4941 5027 6079 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1468 1052 921 591 467 449 396 335
306 246 166 86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 1633 2201 1633
Size: 568 5959
BtgI C'CryG_G
Cuts at: 107 1577 1753 2223 107
Size: 1470 176 470 4411
Fragments arranged by size:
4411 1470 470 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4477 4658 4821 4477
Size: 181 163 6183
Fragments arranged by size:
6183 181 163
BtsI GCAGTG_nn'
Cuts at: 925 1916 1977 3770 925
Size: 991 61 1793 3682
Fragments arranged by size:
3682 1793 991 61
DraI TTT'AAA
Cuts at: 3526 3526
Size: 6527
DraIII CAC_nnn'GTG
Cuts at: 513 513
Size: 6527
DrdI GACnn_nn'nnGTC
Cuts at: 237 4233 5577 237
Size: 3996 1344 1187
Fragments arranged by size:
3996 1344 1187
EaeI y'GGCC_r
Cuts at: 191 1404 1675 2438 2569 2658 2996 3167 4112
Size: 1213 271 763 131 89 338 171 945
Cuts at: 4112 4286 4677 4704 6049 191
Size: 174 391 27 1345 669
Fragments arranged by size:
1345 1213 945 763 669 391 338 271
174 171 131 89 27
EagI C'GGCC_G
Cuts at: 4112 4112
Size: 6527
EarI CTCTTCn'nnn_
Cuts at: 151 577 2257 2300 2363 4550 4760 151
Size: 426 1680 43 63 2187 210 1918
Fragments arranged by size:
2187 1918 1680 426 210 63 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1537 4735 5477 5623 6337 1537
Size: 3198 742 146 714 1727
Fragments arranged by size:
3198 1727 742 714 146
EcoICRI GAG'CTC
Cuts at: 3291 3539 3291
Size: 248 6279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 497 1017 1042 1207 2024 2310 2387 2716
Size: 181 520 25 165 817 286 77 329
Cuts at: 2716 4352 4784 5137 5799 316
Size: 1636 432 353 662 1044
Fragments arranged by size:
1636 1044 817 662 520 432 353 329
286 181 165 77 25
EcoO109I rG'GnC_Cy
Cuts at: 279 945 1697 1705 2067 2340 2373 2549 2634
Size: 666 752 8 362 273 33 176 85
Cuts at: 2634 2822 2907 3042 3666 279
Size: 188 85 135 624 3140
Fragments arranged by size:
3140 752 666 624 362 273 188 176
135 85 85 33 8
EcoRI G'AATT_C
Cuts at: 3531 3531
Size: 6527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4546 4546
Size: 6527
FspI TGC'GCA
Cuts at: 319 4308 319
Size: 3989 2538
FspAI rTGC'GCAy
Cuts at: 319 319
Size: 6527
HaeII r_GCGC'y
Cuts at: 222 725 765 1422 3961 4209 5439 6210 222
Size: 503 40 657 2539 248 1230 771 539
Fragments arranged by size:
2539 1230 771 657 539 503 248 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3132 3164 3202 3234 4369 4399 4401 4431 3132
Size: 32 38 32 1135 30 2 30 5228
Fragments arranged by size:
5228 1135 38 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1589 3566 1589
Size: 1977 4550
HindIII A'AGCT_T
Cuts at: 1019 1019
Size: 6527
KasI G'GCGC_C
Cuts at: 3957 4205 3957
Size: 248 6279
KpnI G_GTAC'C
Cuts at: 3547 3547
Size: 6527
MluI A'CGCG_T
Cuts at: 5960 5960
Size: 6527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1739 3048 5286 5470 1739
Size: 1309 2238 184 2796
Fragments arranged by size:
2796 2238 1309 184
MscI TGG'CCA
Cuts at: 193 3169 4288 193
Size: 2976 1119 2432
Fragments arranged by size:
2976 2432 1119
MslI CAynn'nnrTG
Cuts at: 460 809 824 1597 1717 2716 4516 4643 460
Size: 349 15 773 120 999 1800 127 2344
Fragments arranged by size:
2344 1800 999 773 349 127 120 15
MspA1I CmG'CkG
Cuts at: 532 869 1228 1333 1509 1668 1959 2091 2365
Size: 337 359 105 176 159 291 132 274
Cuts at: 2365 2388 2973 3511 3638 3952 4312 5096 5341
Size: 23 585 538 127 314 360 784 245
Cuts at: 5341 532
Size: 1718
Fragments arranged by size:
1718 784 585 538 360 359 337 314
291 274 245 176 159 132 127 105
23
NaeI GCC'GGC
Cuts at: 1417 1675 4708 1417
Size: 258 3033 3236
Fragments arranged by size:
3236 3033 258
NarI GG'CG_CC
Cuts at: 3958 4206 3958
Size: 248 6279
NcoI C'CATG_G
Cuts at: 107 1577 107
Size: 1470 5057
NgoMIV G'CCGG_C
Cuts at: 1415 1673 4706 1415
Size: 258 3033 3236
Fragments arranged by size:
3236 3033 258
NspI r_CATG'y
Cuts at: 1774 2262 3009 3438 3468 3580 4080 5683 1774
Size: 488 747 429 30 112 500 1603 2618
Fragments arranged by size:
2618 1603 747 500 488 429 112 30
PasI CC'CwG_GG
Cuts at: 714 2316 3309 714
Size: 1602 993 3932
Fragments arranged by size:
3932 1602 993
PciI A'CATG_T
Cuts at: 3464 5679 3464
Size: 2215 4312
PflMI CCAn_nnn'nTGG
Cuts at: 113 1497 1583 113
Size: 1384 86 5057
Fragments arranged by size:
5057 1384 86
PmeI GTTT'AAAC
Cuts at: 3526 3526
Size: 6527
PmlI CAC'GTG
Cuts at: 547 2016 547
Size: 1469 5058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3013 3045 3067 3099 4973 5005 3013
Size: 32 22 32 1874 32 4535
Fragments arranged by size:
4535 1874 32 32 32 22
PpuMI rG'GwC_Cy
Cuts at: 2549 2634 2907 3042 2549
Size: 85 273 135 6034
Fragments arranged by size:
6034 273 135 85
PspOMI G'GGCC_C
Cuts at: 279 1992 2340 279
Size: 1713 348 4466
Fragments arranged by size:
4466 1713 348
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2824 2856 3472 3504 2824
Size: 32 616 32 5847
Fragments arranged by size:
5847 616 32 32
PstI C_TGCA'G
Cuts at: 650 886 1233 2333 2537 2582 3574 4259 650
Size: 236 347 1100 204 45 992 685 2918
Fragments arranged by size:
2918 1100 992 685 347 236 204 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6527
PvuII CAG'CTG
Cuts at: 1228 1333 1668 1959 2091 2365 2388 2973 3511
Size: 105 335 291 132 274 23 585 538
Cuts at: 3511 3952 4312 1228
Size: 441 360 3443
Fragments arranged by size:
3443 585 538 441 360 335 291 274
132 105 23
RsrII CG'GwC_CG
Cuts at: 4722 4722
Size: 6527
SacI G_AGCT'C
Cuts at: 3293 3541 3293
Size: 248 6279
SalI G'TCGA_C
Cuts at: 1587 3564 1587
Size: 1977 4550
SapI GCTCTTCn'nnn_
Cuts at: 577 4550 4760 577
Size: 3973 210 2344
Fragments arranged by size:
3973 2344 210
SbfI CC_TGCA'GG
Cuts at: 3574 3574
Size: 6527
ScaI AGT'ACT
Cuts at: 6044 6044
Size: 6527
SexAI A'CCwGG_T
Cuts at: 365 365
Size: 6527
SfcI C'TryA_G
Cuts at: 33 646 684 882 1229 2329 2533 2578 3186
Size: 613 38 198 347 1100 204 45 608
Cuts at: 3186 3421 3570 4255 5223 5414 33
Size: 235 149 685 968 191 1146
Fragments arranged by size:
1146 1100 968 685 613 608 347 235
204 198 191 149 45 38
SfoI GGC'GCC
Cuts at: 3959 4207 3959
Size: 248 6279
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6527
SmaI CCC'GGG
Cuts at: 330 1824 3549 330
Size: 1494 1725 3308
Fragments arranged by size:
3308 1725 1494
SmlI C'TyrA_G
Cuts at: 2933 3682 4597 5311 5573 2933
Size: 749 915 714 262 3887
Fragments arranged by size:
3887 915 749 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6527
SphI G_CATG'C
Cuts at: 2262 3009 3438 3580 4080 2262
Size: 747 429 142 500 4709
Fragments arranged by size:
4709 747 500 429 142
SrfI GCCC'GGGC
Cuts at: 330 1824 330
Size: 1494 5033
StuI AGG'CCT
Cuts at: 880 2199 3454 880
Size: 1319 1255 3953
Fragments arranged by size:
3953 1319 1255
StyI C'CwwG_G
Cuts at: 107 782 1301 1577 1814 3252 3661 4015 107
Size: 675 519 276 237 1438 409 354 2619
Fragments arranged by size:
2619 1438 675 519 409 354 276 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1109 1963 4110 4243 4702 4911 1109
Size: 854 2147 133 459 209 2725
Fragments arranged by size:
2725 2147 854 459 209 133
TatI w'GTAC_w
Cuts at: 534 6042 534
Size: 5508 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1206 1238 1454 1486 1716 1748 2764 2796 6044
Size: 32 216 32 230 32 1016 32 3248
Cuts at: 6044 6076 1206
Size: 32 1657
Fragments arranged by size:
3248 1657 1016 230 216 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 499 4324 499
Size: 3825 2702
XbaI T'CTAG_A
Cuts at: 3558 3558
Size: 6527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 147 1072 1308 2139 2609 4022 147
Size: 925 236 831 470 1413 2652
Fragments arranged by size:
2652 1413 925 831 470 236
XhoI C'TCGA_G
Cuts at: 2933 2933
Size: 6527
XmaI C'CCGG_G
Cuts at: 328 1822 3547 328
Size: 1494 1725 3308
Fragments arranged by size:
3308 1725 1494
XmnI GAAnn'nnTTC
Cuts at: 1240 3028 1240
Size: 1788 4739
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BfrBI BmtI
BsiWI BspEI BsrGI BstEII BstZ17I ClaI CspCI EcoNI
EcoRV FseI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SnaBI SspI SwaI ZraI