Restriction map for
pF1KB9213
(Circular) (Six-base) MAPSORT of: pF1KB9213.seq Check: 5424 from: 1 to: 5630
pF1KB9213 5630 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2668 2668
Size: 5630
Acc65I G'GTAC_C
Cuts at: 2646 2646
Size: 5630
AclI AA'CG_TT
Cuts at: 2230 5470 2230
Size: 3240 2390
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 191 860 1981 1990 2509 3455 3887 4240
Size: 79 669 1121 9 519 946 432 353
Cuts at: 4240 4902 112
Size: 662 840
Fragments arranged by size:
1121 946 840 669 662 519 432 353
79 9
AflIII A'CryG_T
Cuts at: 2982 4782 5063 2982
Size: 1800 281 3549
Fragments arranged by size:
3549 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 187 685 717 2935 4373 187
Size: 498 32 2218 1438 1444
Fragments arranged by size:
2218 1444 1438 498 32
ApaI G_GGCC'C
Cuts at: 1249 1249
Size: 5630
ApaLI G'TGCA_C
Cuts at: 1673 4468 1673
Size: 2795 2835
ApoI r'AATT_y
Cuts at: 496 2634 4983 5239 496
Size: 2138 2349 256 887
Fragments arranged by size:
2349 2138 887 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5630
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
AvaI C'yCGr_G
Cuts at: 2650 2650
Size: 5630
BamHI G'GATC_C
Cuts at: 2655 2655
Size: 5630
BanI G'GyrC_C
Cuts at: 435 1090 2646 3060 3308 3343 435
Size: 655 1556 414 248 35 2722
Fragments arranged by size:
2722 1556 655 414 248 35
BanII G_rGCy'C
Cuts at: 596 1249 1322 2644 3674 596
Size: 653 73 1322 1030 2552
Fragments arranged by size:
2552 1322 1030 653 73
BbeI G_GCGC'C
Cuts at: 3064 3312 3064
Size: 248 5382
BbsI GAAGACnn'nnnn_
Cuts at: 932 1752 932
Size: 820 4810
BbvCI CC'TCA_GC
Cuts at: 938 938
Size: 5630
BciVI GTATCCnnnnn_n'
Cuts at: 861 3528 4584 5630 861
Size: 2667 1056 1046 861
Fragments arranged by size:
2667 1056 1046 861
BclI T'GATC_A
Cuts at: 3150 3150
Size: 5630
BglII A'GATC_T
Cuts at: 879 1173 1878 3145 5182 879
Size: 294 705 1267 2037 1327
Fragments arranged by size:
2037 1327 1267 705 294
BlpI GC'TnA_GC
Cuts at: 1700 2742 1700
Size: 1042 4588
Bme1580I G_kGCm'C
Cuts at: 631 1249 1256 1677 3255 3348 4472 631
Size: 618 7 421 1578 93 1124 1789
Fragments arranged by size:
1789 1578 1124 618 421 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 1250 2842 2923 3258 5402 1250
Size: 1592 81 335 2144 1478
Fragments arranged by size:
2144 1592 1478 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1175 2260 3997 1175
Size: 1085 1737 2808
Fragments arranged by size:
2808 1737 1085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1170 1170
Size: 5630
Bpu10I CC'TnA_GC
Cuts at: 756 938 2391 756
Size: 182 1453 3995
Fragments arranged by size:
3995 1453 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1584 2806 3685 4399 4697 348
Size: 1236 1222 879 714 298 1281
Fragments arranged by size:
1281 1236 1222 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 610 1399 5334 610
Size: 789 3935 906
Fragments arranged by size:
3935 906 789
BsaAI yAC'GTr
Cuts at: 1378 2983 1378
Size: 1605 4025
BsaBI GATnn'nnATC
Cuts at: 6 3144 5181 6
Size: 3138 2037 455
Fragments arranged by size:
3138 2037 455
BsaHI Gr'CG_yC
Cuts at: 244 3061 3309 4011 5507 244
Size: 2817 248 702 1496 367
Fragments arranged by size:
2817 1496 702 367 248
BsaWI w'CCGG_w
Cuts at: 2819 3340 4018 4429 4576 4850 2819
Size: 521 678 411 147 274 3599
Fragments arranged by size:
3599 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 5630
BseYI C'CCAG_C
Cuts at: 1213 3055 3813 4478 4886 1213
Size: 1842 758 665 408 1957
Fragments arranged by size:
1957 1842 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1355 2165 2194 1355
Size: 810 29 4791
Fragments arranged by size:
4791 810 29
BsiEI CG_ry'CG
Cuts at: 104 3172 3218 4448 104
Size: 3068 46 1230 1286
Fragments arranged by size:
3068 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 596 1677 2608 2644 3422 3612 4472 596
Size: 1081 931 36 778 190 860 1754
Fragments arranged by size:
1754 1081 931 860 778 190 36
BsmI GAATG_Cn'
Cuts at: 1825 1825
Size: 5630
BsmBI CGTCTCn'nnnn_
Cuts at: 1765 1765
Size: 5630
Bsp1286I G_dGCh'C
Cuts at: 596 631 1249 1256 1322 1677 2608 2644 3255
Size: 35 618 7 66 355 931 36 611
Cuts at: 3255 3348 3422 3612 3674 4472 596
Size: 93 74 190 62 798 1754
Fragments arranged by size:
1754 931 798 618 611 355 190 93
74 66 62 36 35 7
BspHI T'CATG_A
Cuts at: 4062 4062
Size: 5630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 146 2680 2894 3196 3577 146
Size: 2534 214 302 381 2199
Fragments arranged by size:
2534 2199 381 302 214
BsrBI CCG'CTC
Cuts at: 50 418 3922 3976 5460 5625 50
Size: 368 3504 54 1484 165 55
Fragments arranged by size:
3504 1484 368 165 55 54
BsrDI GCAATG_nn'
Cuts at: 669 2126 3542 669
Size: 1457 1416 2757
Fragments arranged by size:
2757 1457 1416
BsrFI r'CCGG_y
Cuts at: 467 3628 3809 467
Size: 3161 181 2288
Fragments arranged by size:
3161 2288 181
BsrGI T'GTAC_A
Cuts at: 849 849
Size: 5630
BssSI C'ACGA_G
Cuts at: 1670 1837 3901 4609 1670
Size: 167 2064 708 2691
Fragments arranged by size:
2691 2064 708 167
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 2373 3007 1378
Size: 995 634 4001
Fragments arranged by size:
4001 995 634
BstBI TT'CG_AA
Cuts at: 3991 3991
Size: 5630
BstYI r'GATC_y
Cuts at: 1 879 1173 1878 2655 3122 3145 3480 3726
Size: 878 294 705 777 467 23 335 246
Cuts at: 3726 4032 4044 4130 5182 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 878 777 705 467 449 335 306
294 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1628 1628
Size: 5630
BtgI C'CryG_G
Cuts at: 107 1274 2348 107
Size: 1167 1074 3389
Fragments arranged by size:
3389 1167 1074
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 356 429 1523 2558 3580 3761 3924 356
Size: 73 1094 1035 1022 181 163 2062
Fragments arranged by size:
2062 1094 1035 1022 181 163 73
BtsI GCAGTG_nn'
Cuts at: 1758 1807 2873 1758
Size: 49 1066 4515
Fragments arranged by size:
4515 1066 49
DraI TTT'AAA
Cuts at: 771 1907 2629 771
Size: 1136 722 3772
Fragments arranged by size:
3772 1136 722
DraIII CAC_nnn'GTG
Cuts at: 1378 1378
Size: 5630
DrdI GACnn_nn'nnGTC
Cuts at: 2545 3336 4680 2545
Size: 791 1344 3495
Fragments arranged by size:
3495 1344 791
EaeI y'GGCC_r
Cuts at: 389 1277 3215 3389 3780 3807 5152 389
Size: 888 1938 174 391 27 1345 867
Fragments arranged by size:
1938 1345 888 867 391 174 27
EagI C'GGCC_G
Cuts at: 3215 3215
Size: 5630
EarI CTCTTCn'nnn_
Cuts at: 788 1166 1397 1541 1980 2528 3653 3863 788
Size: 378 231 144 439 548 1125 210 2555
Fragments arranged by size:
2555 1125 548 439 378 231 210 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3838 4580 4726 5440 127
Size: 3711 742 146 714 317
Fragments arranged by size:
3711 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 594 2642 594
Size: 2048 3582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 191 860 1170 1981 1990 2509 3455 3887
Size: 79 669 310 811 9 519 946 432
Cuts at: 3887 4240 4902 112
Size: 353 662 840
Fragments arranged by size:
946 840 811 669 662 519 432 353
310 79 9
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 5630
EcoO109I rG'GnC_Cy
Cuts at: 135 721 1245 1246 2769 135
Size: 586 524 1 1523 2996
Fragments arranged by size:
2996 1523 586 524 1
EcoRI G'AATT_C
Cuts at: 2634 2634
Size: 5630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3649 3649
Size: 5630
FspI TGC'GCA
Cuts at: 3411 3411
Size: 5630
HaeII r_GCGC'y
Cuts at: 3064 3312 4542 5313 3064
Size: 248 1230 771 3381
Fragments arranged by size:
3381 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 208 240 813 845 1222 1254 1296
Size: 32 13 32 573 32 377 32 42
Cuts at: 1296 1328 3472 3502 3504 3534 163
Size: 32 2144 30 2 30 2259
Fragments arranged by size:
2259 2144 573 377 42 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 242 1686 2669 242
Size: 1444 983 3203
Fragments arranged by size:
3203 1444 983
HindIII A'AGCT_T
Cuts at: 1963 1963
Size: 5630
KasI G'GCGC_C
Cuts at: 3060 3308 3060
Size: 248 5382
KpnI G_GTAC'C
Cuts at: 2650 2650
Size: 5630
MfeI C'AATT_G
Cuts at: 1864 1864
Size: 5630
MluI A'CGCG_T
Cuts at: 5063 5063
Size: 5630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 2149 4389 4573 563
Size: 1586 2240 184 1620
Fragments arranged by size:
2240 1620 1586 184
MscI TGG'CCA
Cuts at: 1279 3391 1279
Size: 2112 3518
MslI CAynn'nnrTG
Cuts at: 1273 1328 1949 3619 3746 1273
Size: 55 621 1670 127 3157
Fragments arranged by size:
3157 1670 621 127 55
MspA1I CmG'CkG
Cuts at: 1369 1873 2070 2486 2741 3055 3415 4199 4444
Size: 504 197 416 255 314 360 784 245
Cuts at: 4444 1369
Size: 2555
Fragments arranged by size:
2555 784 504 416 360 314 255 245
197
NaeI GCC'GGC
Cuts at: 3811 3811
Size: 5630
NarI GG'CG_CC
Cuts at: 3061 3309 3061
Size: 248 5382
NcoI C'CATG_G
Cuts at: 107 1274 2348 107
Size: 1167 1074 3389
Fragments arranged by size:
3389 1167 1074
NdeI CA'TA_TG
Cuts at: 483 483
Size: 5630
NgoMIV G'CCGG_C
Cuts at: 3809 3809
Size: 5630
NspI r_CATG'y
Cuts at: 1800 2683 3183 4786 1800
Size: 883 500 1603 2644
Fragments arranged by size:
2644 1603 883 500
PasI CC'CwG_GG
Cuts at: 249 249
Size: 5630
PciI A'CATG_T
Cuts at: 4782 4782
Size: 5630
PflMI CCAn_nnn'nTGG
Cuts at: 1087 1087
Size: 5630
PfoI T'CCnGG_A
Cuts at: 946 946
Size: 5630
PmeI GTTT'AAAC
Cuts at: 2629 2629
Size: 5630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 5598
PpuMI rG'GwC_Cy
Cuts at: 135 721 135
Size: 586 5044
PspOMI G'GGCC_C
Cuts at: 1245 1245
Size: 5630
PstI C_TGCA'G
Cuts at: 143 1878 2677 3362 143
Size: 1735 799 685 2411
Fragments arranged by size:
2411 1735 799 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5630
PvuII CAG'CTG
Cuts at: 1873 2070 2486 3055 3415 1873
Size: 197 416 569 360 4088
Fragments arranged by size:
4088 569 416 360 197
RsrII CG'GwC_CG
Cuts at: 3825 3825
Size: 5630
SacI G_AGCT'C
Cuts at: 596 2644 596
Size: 2048 3582
SalI G'TCGA_C
Cuts at: 2667 2667
Size: 5630
SapI GCTCTTCn'nnn_
Cuts at: 2528 3653 3863 2528
Size: 1125 210 4295
Fragments arranged by size:
4295 1125 210
SbfI CC_TGCA'GG
Cuts at: 143 2677 143
Size: 2534 3096
ScaI AGT'ACT
Cuts at: 5147 5147
Size: 5630
SexAI A'CCwGG_T
Cuts at: 1053 1053
Size: 5630
SfcI C'TryA_G
Cuts at: 33 139 174 1874 2673 3358 4326 4517 33
Size: 106 35 1700 799 685 968 191 1146
Fragments arranged by size:
1700 1146 968 799 685 191 106 35
SfoI GGC'GCC
Cuts at: 3062 3310 3062
Size: 248 5382
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
SmaI CCC'GGG
Cuts at: 2652 2652
Size: 5630
SmlI C'TyrA_G
Cuts at: 327 1563 2785 3700 4414 4676 327
Size: 1236 1222 915 714 262 1281
Fragments arranged by size:
1281 1236 1222 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5630
SphI G_CATG'C
Cuts at: 2683 3183 2683
Size: 500 5130
StyI C'CwwG_G
Cuts at: 107 1274 2348 2764 3118 107
Size: 1167 1074 416 354 2619
Fragments arranged by size:
2619 1167 1074 416 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3213 3346 3805 4014 3213
Size: 133 459 209 4829
Fragments arranged by size:
4829 459 209 133
TatI w'GTAC_w
Cuts at: 849 997 1611 5145 849
Size: 148 614 3534 1334
Fragments arranged by size:
3534 1334 614 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 149 181 5147 5179 149
Size: 32 4966 32 600
Fragments arranged by size:
4966 600 32 32
Tth111I GACn'n_nGTC
Cuts at: 220 3427 220
Size: 3207 2423
XbaI T'CTAG_A
Cuts at: 1176 1314 2082 2661 1176
Size: 138 768 579 4145
Fragments arranged by size:
4145 768 579 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 906 1290 3125 906
Size: 384 1835 3411
Fragments arranged by size:
3411 1835 384
XmaI C'CCGG_G
Cuts at: 2650 2650
Size: 5630
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BspEI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI