(Circular) (Six-base) MAPSORT of: pF1KB9239.seq Check: 875 from: 1 to: 7403 pF1KB9239 7403 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 388 388 Size: 7403 AatII G_ACGT'C Cuts at: 374 3416 3977 374 Size: 3042 561 3800 Fragments arranged by size: 3800 3042 561 AccI GT'mk_AC Cuts at: 1355 3977 4441 1355 Size: 2622 464 4317 Fragments arranged by size: 4317 2622 464 Acc65I G'GTAC_C Cuts at: 4419 4419 Size: 7403 AclI AA'CG_TT Cuts at: 781 7243 781 Size: 6462 941 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 331 464 813 2707 2755 3916 3927 4306 5228 Size: 133 349 1894 48 1161 11 379 922 Cuts at: 5228 5660 6013 6675 331 Size: 432 353 662 1059 Fragments arranged by size: 1894 1161 1059 922 662 432 379 353 349 133 48 11 AflII C'TTAA_G Cuts at: 526 526 Size: 7403 AflIII A'CryG_T Cuts at: 4755 6555 6836 4755 Size: 1800 281 5322 Fragments arranged by size: 5322 1800 281 AhdI GACnn_n'nnGTC Cuts at: 874 874 Size: 7403 AleI CACnn'nnGTG Cuts at: 3002 3002 Size: 7403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1577 1577 Size: 7403 AlwNI CAG_nnn'CTG Cuts at: 1497 1663 2157 3321 3831 3894 4708 6146 1497 Size: 166 494 1164 510 63 814 1438 2754 Fragments arranged by size: 2754 1438 1164 814 510 494 166 63 ApaLI G'TGCA_C Cuts at: 6241 6241 Size: 7403 ApoI r'AATT_y Cuts at: 232 460 473 625 811 991 1393 4407 6756 Size: 228 13 152 186 180 402 3014 2349 Cuts at: 6756 7012 232 Size: 256 623 Fragments arranged by size: 3014 2349 623 402 256 228 186 180 152 13 AseI AT'TA_AT Cuts at: 20 20 Size: 7403 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7403 AvaI C'yCGr_G Cuts at: 1314 2193 3383 4344 4394 4423 1314 Size: 879 1190 961 50 29 4294 Fragments arranged by size: 4294 1190 961 879 50 29 BamHI G'GATC_C Cuts at: 882 2197 3572 4428 882 Size: 1315 1375 856 3857 Fragments arranged by size: 3857 1375 1315 856 BanI G'GyrC_C Cuts at: 3324 4088 4228 4360 4419 4833 5081 5116 3324 Size: 764 140 132 59 414 248 35 5611 Fragments arranged by size: 5611 764 414 248 140 132 59 35 BanII G_rGCy'C Cuts at: 1204 2642 3797 3823 3851 4155 4417 5447 1204 Size: 1438 1155 26 28 304 262 1030 3160 Fragments arranged by size: 3160 1438 1155 1030 304 262 28 26 BbeI G_GCGC'C Cuts at: 4837 5085 4837 Size: 248 7155 BbsI GAAGACnn'nnnn_ Cuts at: 873 1133 2270 873 Size: 260 1137 6006 Fragments arranged by size: 6006 1137 260 BbvCI CC'TCA_GC Cuts at: 1798 2242 1798 Size: 444 6959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1702 1736 2382 2416 1702 Size: 34 646 34 6689 Fragments arranged by size: 6689 646 34 34 BciVI GTATCCnnnnn_n' Cuts at: 804 2103 4401 5301 6357 7403 804 Size: 1299 2298 900 1056 1046 804 Fragments arranged by size: 2298 1299 1056 1046 900 804 BclI T'GATC_A Cuts at: 3425 4923 3425 Size: 1498 5905 BfrBI ATG'CAT Cuts at: 4012 4012 Size: 7403 BglII A'GATC_T Cuts at: 2049 4918 6955 2049 Size: 2869 2037 2497 Fragments arranged by size: 2869 2497 2037 BlpI GC'TnA_GC Cuts at: 3143 4515 3143 Size: 1372 6031 Bme1580I G_kGCm'C Cuts at: 846 3329 3506 3888 4231 5028 5121 6245 846 Size: 2483 177 382 343 797 93 1124 2004 Fragments arranged by size: 2483 2004 1124 797 382 343 177 93 BmgBI CAC'GTC Cuts at: 1380 1485 1980 2606 3090 3201 3339 1380 Size: 105 495 626 484 111 138 5444 Fragments arranged by size: 5444 626 495 484 138 111 105 BmrI ACTGGGnnnn_n' Cuts at: 1368 2695 4615 4696 5031 7175 1368 Size: 1327 1920 81 335 2144 1596 Fragments arranged by size: 2144 1920 1596 1327 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1146 5770 1146 Size: 4624 2779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 202 632 1460 2274 2449 2822 3054 3730 202 Size: 430 828 814 175 373 232 676 3875 Fragments arranged by size: 3875 828 814 676 430 373 232 175 Bpu10I CC'TnA_GC Cuts at: 1798 2242 3391 3817 1798 Size: 444 1149 426 5384 Fragments arranged by size: 5384 1149 444 426 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 715 2161 2212 4579 5458 6172 6470 715 Size: 1446 51 2367 879 714 298 1648 Fragments arranged by size: 2367 1648 1446 879 714 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 2092 3256 7107 2092 Size: 1164 3851 2388 Fragments arranged by size: 3851 2388 1164 BsaAI yAC'GTr Cuts at: 1458 2079 4756 1458 Size: 621 2677 4105 Fragments arranged by size: 4105 2677 621 BsaBI GATnn'nnATC Cuts at: 6 580 3172 4917 6954 6 Size: 574 2592 1745 2037 455 Fragments arranged by size: 2592 2037 1745 574 455 BsaHI Gr'CG_yC Cuts at: 371 1645 2262 3413 3974 4834 5082 5784 7280 Size: 1274 617 1151 561 860 248 702 1496 Cuts at: 7280 371 Size: 494 Fragments arranged by size: 1496 1274 1151 860 702 617 561 494 248 BsaWI w'CCGG_w Cuts at: 1985 4592 5113 5791 6202 6349 6623 1985 Size: 2607 521 678 411 147 274 2765 Fragments arranged by size: 2765 2607 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 484 514 1997 2027 484 Size: 30 1483 30 5860 Fragments arranged by size: 5860 1483 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1786 2259 2397 3136 3358 3478 4018 1786 Size: 473 138 739 222 120 540 5171 Fragments arranged by size: 5171 739 540 473 222 138 120 BseYI C'CCAG_C Cuts at: 2905 3417 3824 4171 4254 4828 5586 6251 6659 Size: 512 407 347 83 574 758 665 408 Cuts at: 6659 2905 Size: 3649 Fragments arranged by size: 3649 758 665 574 512 408 407 347 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 328 481 1521 1537 1933 3112 3213 3262 3427 Size: 153 1040 16 396 1179 101 49 165 Cuts at: 3427 3774 328 Size: 347 3957 Fragments arranged by size: 3957 1179 1040 396 347 165 153 101 49 16 BsiEI CG_ry'CG Cuts at: 104 2471 2797 4945 4991 6221 104 Size: 2367 326 2148 46 1230 1286 Fragments arranged by size: 2367 2148 1286 1230 326 46 BsiHKAI G_wGCw'C Cuts at: 2078 3842 4222 4417 5195 5385 6245 2078 Size: 1764 380 195 778 190 860 3236 Fragments arranged by size: 3236 1764 860 778 380 195 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1636 3706 1636 Size: 2070 5333 Bsp1286I G_dGCh'C Cuts at: 846 1204 2078 2642 3329 3506 3797 3823 3842 Size: 358 874 564 687 177 291 26 19 Cuts at: 3842 3851 3888 4155 4222 4231 4417 5028 5121 Size: 9 37 267 67 9 186 611 93 Cuts at: 5121 5195 5385 5447 6245 846 Size: 74 190 62 798 2004 Fragments arranged by size: 2004 874 798 687 611 564 358 291 267 190 186 177 93 74 67 62 37 26 19 9 9 BspHI T'CATG_A Cuts at: 2780 3854 5835 2780 Size: 1074 1981 4348 Fragments arranged by size: 4348 1981 1074 BspMI ACCTGCnnnn'nnnn_ Cuts at: 388 503 2074 2165 2285 3449 4453 4667 4969 Size: 115 1571 91 120 1164 1004 214 302 Cuts at: 4969 5350 388 Size: 381 2441 Fragments arranged by size: 2441 1571 1164 1004 381 302 214 120 115 91 BsrBI CCG'CTC Cuts at: 50 294 3449 5695 5749 7233 7398 50 Size: 244 3155 2246 54 1484 165 55 Fragments arranged by size: 3155 2246 1484 244 165 55 54 BsrDI GCAATG_nn' Cuts at: 1259 1742 3137 4329 5315 1259 Size: 483 1395 1192 986 3347 Fragments arranged by size: 3347 1395 1192 986 483 BsrFI r'CCGG_y Cuts at: 151 211 263 1606 2796 3566 5401 5582 151 Size: 60 52 1343 1190 770 1835 181 1972 Fragments arranged by size: 1972 1835 1343 1190 770 181 60 52 BssSI C'ACGA_G Cuts at: 346 2071 4123 5674 6382 346 Size: 1725 2052 1551 708 1367 Fragments arranged by size: 2052 1725 1551 1367 708 BstAPI GCAn_nnn'nTGC Cuts at: 3956 4780 3956 Size: 824 6579 BstBI TT'CG_AA Cuts at: 2103 2940 5764 2103 Size: 837 2824 3742 Fragments arranged by size: 3742 2824 837 BstXI CCAn_nnnn'nTGG Cuts at: 3921 3921 Size: 7403 BstYI r'GATC_y Cuts at: 1 317 882 2049 2197 2648 3356 3572 4428 Size: 316 565 1167 148 451 708 216 856 Cuts at: 4428 4895 4918 5253 5499 5805 5817 5903 6955 Size: 467 23 335 246 306 12 86 1052 Cuts at: 6955 1 Size: 449 Fragments arranged by size: 1167 1052 856 708 565 467 451 449 335 316 306 246 216 148 86 23 12 Bsu36I CC'TnA_GG Cuts at: 3130 3130 Size: 7403 BtgI C'CryG_G Cuts at: 2027 3257 3268 3302 3368 3404 3506 3642 3675 Size: 1230 11 34 66 36 102 136 33 Cuts at: 3675 3702 3868 3920 2027 Size: 27 166 52 5510 Fragments arranged by size: 5510 1230 166 136 102 66 52 36 34 33 27 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1121 5353 5534 5697 1121 Size: 4232 181 163 2827 Fragments arranged by size: 4232 2827 181 163 BtsI GCAGTG_nn' Cuts at: 174 2460 4646 174 Size: 2286 2186 2931 Fragments arranged by size: 2931 2286 2186 ClaI AT'CG_AT Cuts at: 1322 1322 Size: 7403 DraI TTT'AAA Cuts at: 630 4402 630 Size: 3772 3631 DrdI GACnn_nn'nnGTC Cuts at: 5109 6453 5109 Size: 1344 6059 EaeI y'GGCC_r Cuts at: 1374 1473 1586 1697 1958 2187 2468 2762 3113 Size: 99 113 111 261 229 281 294 351 Cuts at: 3113 3635 3678 3776 4326 4988 5162 5553 5580 Size: 522 43 98 550 662 174 391 27 Cuts at: 5580 6925 1374 Size: 1345 1852 Fragments arranged by size: 1852 1345 662 550 522 391 351 294 281 261 229 174 113 111 99 98 43 27 EagI C'GGCC_G Cuts at: 2468 4988 2468 Size: 2520 4883 EarI CTCTTCn'nnn_ Cuts at: 440 886 2131 2630 5426 5636 440 Size: 446 1245 499 2796 210 2207 Fragments arranged by size: 2796 2207 1245 499 446 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3598 5611 6353 6499 7213 3598 Size: 2013 742 146 714 3788 Fragments arranged by size: 3788 2013 742 714 146 EcoICRI GAG'CTC Cuts at: 4415 4415 Size: 7403 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 202 331 464 632 813 1460 2274 2449 2707 Size: 129 133 168 181 647 814 175 258 Cuts at: 2707 2755 2822 3054 3730 3916 3927 4306 5228 Size: 48 67 232 676 186 11 379 922 Cuts at: 5228 5660 6013 6675 202 Size: 432 353 662 930 Fragments arranged by size: 930 922 814 676 662 647 432 379 353 258 232 186 181 175 168 133 129 67 48 11 EcoNI CCTnn'n_nnAGG Cuts at: 346 1665 4078 346 Size: 1319 2413 3671 Fragments arranged by size: 3671 2413 1319 EcoO109I rG'GnC_Cy Cuts at: 746 3126 3387 4542 746 Size: 2380 261 1155 3607 Fragments arranged by size: 3607 2380 1155 261 EcoRI G'AATT_C Cuts at: 4407 4407 Size: 7403 EcoRV GAT'ATC Cuts at: 1043 1043 Size: 7403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3957 5422 3957 Size: 1465 5938 FspI TGC'GCA Cuts at: 3496 5184 3496 Size: 1688 5715 FspAI rTGC'GCAy Cuts at: 3496 3496 Size: 7403 HaeII r_GCGC'y Cuts at: 2023 3277 3321 4837 5085 6315 7086 2023 Size: 1254 44 1516 248 1230 771 2340 Fragments arranged by size: 2340 1516 1254 1230 771 248 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 860 892 2114 2146 2464 2496 2590 2622 3964 Size: 32 1222 32 318 32 94 32 1342 Cuts at: 3964 3996 5245 5275 5277 5307 860 Size: 32 1249 30 2 30 2956 Fragments arranged by size: 2956 1342 1249 1222 318 94 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 675 804 1224 3672 4442 675 Size: 129 420 2448 770 3636 Fragments arranged by size: 3636 2448 770 420 129 HindIII A'AGCT_T Cuts at: 3939 3939 Size: 7403 HpaI GTT'AAC Cuts at: 1224 1224 Size: 7403 KasI G'GCGC_C Cuts at: 4833 5081 4833 Size: 248 7155 KpnI G_GTAC'C Cuts at: 4423 4423 Size: 7403 MluI A'CGCG_T Cuts at: 6836 6836 Size: 7403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1235 1949 2166 6162 6346 1235 Size: 714 217 3996 184 2292 Fragments arranged by size: 3996 2292 714 217 184 MscI TGG'CCA Cuts at: 1376 1475 1588 2189 2764 3115 3637 3778 4328 Size: 99 113 601 575 351 522 141 550 Cuts at: 4328 5164 1376 Size: 836 3615 Fragments arranged by size: 3615 836 601 575 550 522 351 141 113 99 MslI CAynn'nnrTG Cuts at: 112 329 3002 4234 5392 5519 112 Size: 217 2673 1232 1158 127 1996 Fragments arranged by size: 2673 1996 1232 1158 217 127 MspA1I CmG'CkG Cuts at: 799 2655 3060 3102 3221 3398 3704 3831 4358 Size: 1856 405 42 119 177 306 127 527 Cuts at: 4358 4514 4828 5188 5972 6217 799 Size: 156 314 360 784 245 1985 Fragments arranged by size: 1985 1856 784 527 405 360 314 306 245 177 156 127 119 42 NaeI GCC'GGC Cuts at: 265 1608 5584 265 Size: 1343 3976 2084 Fragments arranged by size: 3976 2084 1343 NarI GG'CG_CC Cuts at: 4834 5082 4834 Size: 248 7155 NcoI C'CATG_G Cuts at: 3368 3404 3920 3368 Size: 36 516 6851 Fragments arranged by size: 6851 516 36 NgoMIV G'CCGG_C Cuts at: 263 1606 5582 263 Size: 1343 3976 2084 Fragments arranged by size: 3976 2084 1343 NsiI A_TGCA'T Cuts at: 4014 4014 Size: 7403 NspI r_CATG'y Cuts at: 1862 1878 4456 4956 6559 1862 Size: 16 2578 500 1603 2706 Fragments arranged by size: 2706 2578 1603 500 16 PasI CC'CwG_GG Cuts at: 4074 4074 Size: 7403 PciI A'CATG_T Cuts at: 6555 6555 Size: 7403 PflMI CCAn_nnn'nTGG Cuts at: 760 3321 760 Size: 2561 4842 PfoI T'CCnGG_A Cuts at: 1328 1526 2200 3031 3209 1328 Size: 198 674 831 178 5522 Fragments arranged by size: 5522 831 674 198 178 PmeI GTTT'AAAC Cuts at: 4402 4402 Size: 7403 PmlI CAC'GTG Cuts at: 1458 1458 Size: 7403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5849 5881 5849 Size: 32 7371 PpuMI rG'GwC_Cy Cuts at: 3126 3387 3126 Size: 261 7142 PshAI GACnn'nnGTC Cuts at: 370 370 Size: 7403 PspXI vC'TCGA_Gb Cuts at: 4344 4344 Size: 7403 PstI C_TGCA'G Cuts at: 1190 2162 3074 3356 4450 5135 1190 Size: 972 912 282 1094 685 3458 Fragments arranged by size: 3458 1094 972 912 685 282 PvuI CG_AT'CG Cuts at: 104 104 Size: 7403 PvuII CAG'CTG Cuts at: 2655 3060 3102 3831 4828 5188 2655 Size: 405 42 729 997 360 4870 Fragments arranged by size: 4870 997 729 405 360 42 RsrII CG'GwC_CG Cuts at: 5598 5598 Size: 7403 SacI G_AGCT'C Cuts at: 4417 4417 Size: 7403 SacII CC_GC'GG Cuts at: 3705 3705 Size: 7403 SalI G'TCGA_C Cuts at: 4440 4440 Size: 7403 SapI GCTCTTCn'nnn_ Cuts at: 2630 5426 5636 2630 Size: 2796 210 4397 Fragments arranged by size: 4397 2796 210 SbfI CC_TGCA'GG Cuts at: 4450 4450 Size: 7403 ScaI AGT'ACT Cuts at: 6920 6920 Size: 7403 SfcI C'TryA_G Cuts at: 33 1186 1291 2158 3070 3352 4446 5131 6099 Size: 1153 105 867 912 282 1094 685 968 Cuts at: 6099 6290 33 Size: 191 1146 Fragments arranged by size: 1153 1146 1094 968 912 867 685 282 191 105 SfoI GGC'GCC Cuts at: 4835 5083 4835 Size: 248 7155 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7403 SgrAI Cr'CCGG_yG Cuts at: 151 151 Size: 7403 SmaI CCC'GGG Cuts at: 3385 4396 4425 3385 Size: 1011 29 6363 Fragments arranged by size: 6363 1011 29 SmlI C'TyrA_G Cuts at: 526 694 1314 2176 2227 4344 4558 5473 6187 Size: 168 620 862 51 2117 214 915 714 Cuts at: 6187 6449 526 Size: 262 1480 Fragments arranged by size: 2117 1480 915 862 714 620 262 214 168 51 SpeI A'CTAG_T Cuts at: 67 67 Size: 7403 SphI G_CATG'C Cuts at: 4456 4956 4456 Size: 500 6903 SspI AAT'ATT Cuts at: 1281 1281 Size: 7403 StuI AGG'CCT Cuts at: 1184 4112 4336 1184 Size: 2928 224 4251 Fragments arranged by size: 4251 2928 224 StyI C'CwwG_G Cuts at: 1803 2486 3368 3404 3920 4157 4537 4891 1803 Size: 683 882 36 516 237 380 354 4315 Fragments arranged by size: 4315 882 683 516 380 354 237 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2782 2967 3606 4986 5119 5578 5787 2782 Size: 185 639 1380 133 459 209 4398 Fragments arranged by size: 4398 1380 639 459 209 185 133 TatI w'GTAC_w Cuts at: 4133 6918 4133 Size: 2785 4618 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2182 2214 2546 2578 3736 3768 6920 6952 2182 Size: 32 332 32 1158 32 3152 32 2633 Fragments arranged by size: 3152 2633 1158 332 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 255 1174 1769 1981 5200 255 Size: 919 595 212 3219 2458 Fragments arranged by size: 3219 2458 919 595 212 XbaI T'CTAG_A Cuts at: 2631 4434 2631 Size: 1803 5600 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2424 3996 4898 2424 Size: 1572 902 4929 Fragments arranged by size: 4929 1572 902 XhoI C'TCGA_G Cuts at: 1314 4344 1314 Size: 3030 4373 XmaI C'CCGG_G Cuts at: 3383 4394 4423 3383 Size: 1011 29 6363 Fragments arranged by size: 6363 1011 29 XmnI GAAnn'nnTTC Cuts at: 3191 3191 Size: 7403 ZraI GAC'GTC Cuts at: 372 3414 3975 372 Size: 3042 561 3800 Fragments arranged by size: 3800 3042 561 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AgeI AloI ApaI AscI AvrII BaeI BglI BmtI BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI MfeI NdeI NheI NotI NruI PacI PsiI PspOMI PsrI SanDI SexAI SfiI SnaBI SrfI SwaI