Restriction map for pF1KB9239
 (Circular) (Six-base) MAPSORT of: pF1KB9239.seq  Check: 875  from: 1  to: 7403



pF1KB9239  7403 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    388     388 
   Size:       7403
 
AatII G_ACGT'C

Cuts at:    374    3416    3977     374 
   Size:       3042     561    3800

  Fragments arranged by size: 

                3800    3042     561
 
AccI GT'mk_AC

Cuts at:   1355    3977    4441    1355 
   Size:       2622     464    4317

  Fragments arranged by size: 

                4317    2622     464
 
Acc65I G'GTAC_C

Cuts at:   4419    4419 
   Size:       7403
 
AclI AA'CG_TT

Cuts at:    781    7243     781 
   Size:       6462     941
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    331     464     813    2707    2755    3916    3927    4306    5228 
   Size:        133     349    1894      48    1161      11     379     922

Cuts at:   5228    5660    6013    6675     331 
   Size:        432     353     662    1059

  Fragments arranged by size: 

                1894    1161    1059     922     662     432     379     353
                 349     133      48      11
 
AflII C'TTAA_G

Cuts at:    526     526 
   Size:       7403
 
AflIII A'CryG_T

Cuts at:   4755    6555    6836    4755 
   Size:       1800     281    5322

  Fragments arranged by size: 

                5322    1800     281
 
AhdI GACnn_n'nnGTC

Cuts at:    874     874 
   Size:       7403
 
AleI CACnn'nnGTG

Cuts at:   3002    3002 
   Size:       7403
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1577    1577 
   Size:       7403
 
AlwNI CAG_nnn'CTG

Cuts at:   1497    1663    2157    3321    3831    3894    4708    6146    1497 
   Size:        166     494    1164     510      63     814    1438    2754

  Fragments arranged by size: 

                2754    1438    1164     814     510     494     166      63
            
 
ApaLI G'TGCA_C

Cuts at:   6241    6241 
   Size:       7403
 
ApoI r'AATT_y

Cuts at:    232     460     473     625     811     991    1393    4407    6756 
   Size:        228      13     152     186     180     402    3014    2349

Cuts at:   6756    7012     232 
   Size:        256     623

  Fragments arranged by size: 

                3014    2349     623     402     256     228     186     180
                 152      13
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7403
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7403
 
AvaI C'yCGr_G

Cuts at:   1314    2193    3383    4344    4394    4423    1314 
   Size:        879    1190     961      50      29    4294

  Fragments arranged by size: 

                4294    1190     961     879      50      29
 
BamHI G'GATC_C

Cuts at:    882    2197    3572    4428     882 
   Size:       1315    1375     856    3857

  Fragments arranged by size: 

                3857    1375    1315     856
 
BanI G'GyrC_C

Cuts at:   3324    4088    4228    4360    4419    4833    5081    5116    3324 
   Size:        764     140     132      59     414     248      35    5611

  Fragments arranged by size: 

                5611     764     414     248     140     132      59      35
            
 
BanII G_rGCy'C

Cuts at:   1204    2642    3797    3823    3851    4155    4417    5447    1204 
   Size:       1438    1155      26      28     304     262    1030    3160

  Fragments arranged by size: 

                3160    1438    1155    1030     304     262      28      26
            
 
BbeI G_GCGC'C

Cuts at:   4837    5085    4837 
   Size:        248    7155
 
BbsI GAAGACnn'nnnn_

Cuts at:    873    1133    2270     873 
   Size:        260    1137    6006

  Fragments arranged by size: 

                6006    1137     260
 
BbvCI CC'TCA_GC

Cuts at:   1798    2242    1798 
   Size:        444    6959
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1702    1736    2382    2416    1702 
   Size:         34     646      34    6689

  Fragments arranged by size: 

                6689     646      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    804    2103    4401    5301    6357    7403     804 
   Size:       1299    2298     900    1056    1046     804

  Fragments arranged by size: 

                2298    1299    1056    1046     900     804
 
BclI T'GATC_A

Cuts at:   3425    4923    3425 
   Size:       1498    5905
 
BfrBI ATG'CAT

Cuts at:   4012    4012 
   Size:       7403
 
BglII A'GATC_T

Cuts at:   2049    4918    6955    2049 
   Size:       2869    2037    2497

  Fragments arranged by size: 

                2869    2497    2037
 
BlpI GC'TnA_GC

Cuts at:   3143    4515    3143 
   Size:       1372    6031
 
Bme1580I G_kGCm'C

Cuts at:    846    3329    3506    3888    4231    5028    5121    6245     846 
   Size:       2483     177     382     343     797      93    1124    2004

  Fragments arranged by size: 

                2483    2004    1124     797     382     343     177      93
            
 
BmgBI CAC'GTC

Cuts at:   1380    1485    1980    2606    3090    3201    3339    1380 
   Size:        105     495     626     484     111     138    5444

  Fragments arranged by size: 

                5444     626     495     484     138     111     105
 
BmrI ACTGGGnnnn_n'

Cuts at:   1368    2695    4615    4696    5031    7175    1368 
   Size:       1327    1920      81     335    2144    1596

  Fragments arranged by size: 

                2144    1920    1596    1327     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1146    5770    1146 
   Size:       4624    2779
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    202     632    1460    2274    2449    2822    3054    3730     202 
   Size:        430     828     814     175     373     232     676    3875

  Fragments arranged by size: 

                3875     828     814     676     430     373     232     175
            
 
Bpu10I CC'TnA_GC

Cuts at:   1798    2242    3391    3817    1798 
   Size:        444    1149     426    5384

  Fragments arranged by size: 

                5384    1149     444     426
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    715    2161    2212    4579    5458    6172    6470     715 
   Size:       1446      51    2367     879     714     298    1648

  Fragments arranged by size: 

                2367    1648    1446     879     714     298      51
 
BsaI GGTCTCn'nnnn_

Cuts at:   2092    3256    7107    2092 
   Size:       1164    3851    2388

  Fragments arranged by size: 

                3851    2388    1164
 
BsaAI yAC'GTr

Cuts at:   1458    2079    4756    1458 
   Size:        621    2677    4105

  Fragments arranged by size: 

                4105    2677     621
 
BsaBI GATnn'nnATC

Cuts at:      6     580    3172    4917    6954       6 
   Size:        574    2592    1745    2037     455

  Fragments arranged by size: 

                2592    2037    1745     574     455
 
BsaHI Gr'CG_yC

Cuts at:    371    1645    2262    3413    3974    4834    5082    5784    7280 
   Size:       1274     617    1151     561     860     248     702    1496

Cuts at:   7280     371 
   Size:        494

  Fragments arranged by size: 

                1496    1274    1151     860     702     617     561     494
                 248
 
BsaWI w'CCGG_w

Cuts at:   1985    4592    5113    5791    6202    6349    6623    1985 
   Size:       2607     521     678     411     147     274    2765

  Fragments arranged by size: 

                2765    2607     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    484     514    1997    2027     484 
   Size:         30    1483      30    5860

  Fragments arranged by size: 

                5860    1483      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1786    2259    2397    3136    3358    3478    4018    1786 
   Size:        473     138     739     222     120     540    5171

  Fragments arranged by size: 

                5171     739     540     473     222     138     120
 
BseYI C'CCAG_C

Cuts at:   2905    3417    3824    4171    4254    4828    5586    6251    6659 
   Size:        512     407     347      83     574     758     665     408

Cuts at:   6659    2905 
   Size:       3649

  Fragments arranged by size: 

                3649     758     665     574     512     408     407     347
                  83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    328     481    1521    1537    1933    3112    3213    3262    3427 
   Size:        153    1040      16     396    1179     101      49     165

Cuts at:   3427    3774     328 
   Size:        347    3957

  Fragments arranged by size: 

                3957    1179    1040     396     347     165     153     101
                  49      16
 
BsiEI CG_ry'CG

Cuts at:    104    2471    2797    4945    4991    6221     104 
   Size:       2367     326    2148      46    1230    1286

  Fragments arranged by size: 

                2367    2148    1286    1230     326      46
 
BsiHKAI G_wGCw'C

Cuts at:   2078    3842    4222    4417    5195    5385    6245    2078 
   Size:       1764     380     195     778     190     860    3236

  Fragments arranged by size: 

                3236    1764     860     778     380     195     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1636    3706    1636 
   Size:       2070    5333
 
Bsp1286I G_dGCh'C

Cuts at:    846    1204    2078    2642    3329    3506    3797    3823    3842 
   Size:        358     874     564     687     177     291      26      19

Cuts at:   3842    3851    3888    4155    4222    4231    4417    5028    5121 
   Size:          9      37     267      67       9     186     611      93

Cuts at:   5121    5195    5385    5447    6245     846 
   Size:         74     190      62     798    2004

  Fragments arranged by size: 

                2004     874     798     687     611     564     358     291
                 267     190     186     177      93      74      67      62
                  37      26      19       9       9
 
BspHI T'CATG_A

Cuts at:   2780    3854    5835    2780 
   Size:       1074    1981    4348

  Fragments arranged by size: 

                4348    1981    1074
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    388     503    2074    2165    2285    3449    4453    4667    4969 
   Size:        115    1571      91     120    1164    1004     214     302

Cuts at:   4969    5350     388 
   Size:        381    2441

  Fragments arranged by size: 

                2441    1571    1164    1004     381     302     214     120
                 115      91
 
BsrBI CCG'CTC

Cuts at:     50     294    3449    5695    5749    7233    7398      50 
   Size:        244    3155    2246      54    1484     165      55

  Fragments arranged by size: 

                3155    2246    1484     244     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1259    1742    3137    4329    5315    1259 
   Size:        483    1395    1192     986    3347

  Fragments arranged by size: 

                3347    1395    1192     986     483
 
BsrFI r'CCGG_y

Cuts at:    151     211     263    1606    2796    3566    5401    5582     151 
   Size:         60      52    1343    1190     770    1835     181    1972

  Fragments arranged by size: 

                1972    1835    1343    1190     770     181      60      52
            
 
BssSI C'ACGA_G

Cuts at:    346    2071    4123    5674    6382     346 
   Size:       1725    2052    1551     708    1367

  Fragments arranged by size: 

                2052    1725    1551    1367     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3956    4780    3956 
   Size:        824    6579
 
BstBI TT'CG_AA

Cuts at:   2103    2940    5764    2103 
   Size:        837    2824    3742

  Fragments arranged by size: 

                3742    2824     837
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3921    3921 
   Size:       7403
 
BstYI r'GATC_y

Cuts at:      1     317     882    2049    2197    2648    3356    3572    4428 
   Size:        316     565    1167     148     451     708     216     856

Cuts at:   4428    4895    4918    5253    5499    5805    5817    5903    6955 
   Size:        467      23     335     246     306      12      86    1052

Cuts at:   6955       1 
   Size:        449

  Fragments arranged by size: 

                1167    1052     856     708     565     467     451     449
                 335     316     306     246     216     148      86      23
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   3130    3130 
   Size:       7403
 
BtgI C'CryG_G

Cuts at:   2027    3257    3268    3302    3368    3404    3506    3642    3675 
   Size:       1230      11      34      66      36     102     136      33

Cuts at:   3675    3702    3868    3920    2027 
   Size:         27     166      52    5510

  Fragments arranged by size: 

                5510    1230     166     136     102      66      52      36
                  34      33      27      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1121    5353    5534    5697    1121 
   Size:       4232     181     163    2827

  Fragments arranged by size: 

                4232    2827     181     163
 
BtsI GCAGTG_nn'

Cuts at:    174    2460    4646     174 
   Size:       2286    2186    2931

  Fragments arranged by size: 

                2931    2286    2186
 
ClaI AT'CG_AT

Cuts at:   1322    1322 
   Size:       7403
 
DraI TTT'AAA

Cuts at:    630    4402     630 
   Size:       3772    3631
 
DrdI GACnn_nn'nnGTC

Cuts at:   5109    6453    5109 
   Size:       1344    6059
 
EaeI y'GGCC_r

Cuts at:   1374    1473    1586    1697    1958    2187    2468    2762    3113 
   Size:         99     113     111     261     229     281     294     351

Cuts at:   3113    3635    3678    3776    4326    4988    5162    5553    5580 
   Size:        522      43      98     550     662     174     391      27

Cuts at:   5580    6925    1374 
   Size:       1345    1852

  Fragments arranged by size: 

                1852    1345     662     550     522     391     351     294
                 281     261     229     174     113     111      99      98
                  43      27
 
EagI C'GGCC_G

Cuts at:   2468    4988    2468 
   Size:       2520    4883
 
EarI CTCTTCn'nnn_

Cuts at:    440     886    2131    2630    5426    5636     440 
   Size:        446    1245     499    2796     210    2207

  Fragments arranged by size: 

                2796    2207    1245     499     446     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3598    5611    6353    6499    7213    3598 
   Size:       2013     742     146     714    3788

  Fragments arranged by size: 

                3788    2013     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   4415    4415 
   Size:       7403
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    202     331     464     632     813    1460    2274    2449    2707 
   Size:        129     133     168     181     647     814     175     258

Cuts at:   2707    2755    2822    3054    3730    3916    3927    4306    5228 
   Size:         48      67     232     676     186      11     379     922

Cuts at:   5228    5660    6013    6675     202 
   Size:        432     353     662     930

  Fragments arranged by size: 

                 930     922     814     676     662     647     432     379
                 353     258     232     186     181     175     168     133
                 129      67      48      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:    346    1665    4078     346 
   Size:       1319    2413    3671

  Fragments arranged by size: 

                3671    2413    1319
 
EcoO109I rG'GnC_Cy

Cuts at:    746    3126    3387    4542     746 
   Size:       2380     261    1155    3607

  Fragments arranged by size: 

                3607    2380    1155     261
 
EcoRI G'AATT_C

Cuts at:   4407    4407 
   Size:       7403
 
EcoRV GAT'ATC

Cuts at:   1043    1043 
   Size:       7403
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3957    5422    3957 
   Size:       1465    5938
 
FspI TGC'GCA

Cuts at:   3496    5184    3496 
   Size:       1688    5715
 
FspAI rTGC'GCAy

Cuts at:   3496    3496 
   Size:       7403
 
HaeII r_GCGC'y

Cuts at:   2023    3277    3321    4837    5085    6315    7086    2023 
   Size:       1254      44    1516     248    1230     771    2340

  Fragments arranged by size: 

                2340    1516    1254    1230     771     248      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    860     892    2114    2146    2464    2496    2590    2622    3964 
   Size:         32    1222      32     318      32      94      32    1342

Cuts at:   3964    3996    5245    5275    5277    5307     860 
   Size:         32    1249      30       2      30    2956

  Fragments arranged by size: 

                2956    1342    1249    1222     318      94      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    675     804    1224    3672    4442     675 
   Size:        129     420    2448     770    3636

  Fragments arranged by size: 

                3636    2448     770     420     129
 
HindIII A'AGCT_T

Cuts at:   3939    3939 
   Size:       7403
 
HpaI GTT'AAC

Cuts at:   1224    1224 
   Size:       7403
 
KasI G'GCGC_C

Cuts at:   4833    5081    4833 
   Size:        248    7155
 
KpnI G_GTAC'C

Cuts at:   4423    4423 
   Size:       7403
 
MluI A'CGCG_T

Cuts at:   6836    6836 
   Size:       7403
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1235    1949    2166    6162    6346    1235 
   Size:        714     217    3996     184    2292

  Fragments arranged by size: 

                3996    2292     714     217     184
 
MscI TGG'CCA

Cuts at:   1376    1475    1588    2189    2764    3115    3637    3778    4328 
   Size:         99     113     601     575     351     522     141     550

Cuts at:   4328    5164    1376 
   Size:        836    3615

  Fragments arranged by size: 

                3615     836     601     575     550     522     351     141
                 113      99
 
MslI CAynn'nnrTG

Cuts at:    112     329    3002    4234    5392    5519     112 
   Size:        217    2673    1232    1158     127    1996

  Fragments arranged by size: 

                2673    1996    1232    1158     217     127
 
MspA1I CmG'CkG

Cuts at:    799    2655    3060    3102    3221    3398    3704    3831    4358 
   Size:       1856     405      42     119     177     306     127     527

Cuts at:   4358    4514    4828    5188    5972    6217     799 
   Size:        156     314     360     784     245    1985

  Fragments arranged by size: 

                1985    1856     784     527     405     360     314     306
                 245     177     156     127     119      42
 
NaeI GCC'GGC

Cuts at:    265    1608    5584     265 
   Size:       1343    3976    2084

  Fragments arranged by size: 

                3976    2084    1343
 
NarI GG'CG_CC

Cuts at:   4834    5082    4834 
   Size:        248    7155
 
NcoI C'CATG_G

Cuts at:   3368    3404    3920    3368 
   Size:         36     516    6851

  Fragments arranged by size: 

                6851     516      36
 
NgoMIV G'CCGG_C

Cuts at:    263    1606    5582     263 
   Size:       1343    3976    2084

  Fragments arranged by size: 

                3976    2084    1343
 
NsiI A_TGCA'T

Cuts at:   4014    4014 
   Size:       7403
 
NspI r_CATG'y

Cuts at:   1862    1878    4456    4956    6559    1862 
   Size:         16    2578     500    1603    2706

  Fragments arranged by size: 

                2706    2578    1603     500      16
 
PasI CC'CwG_GG

Cuts at:   4074    4074 
   Size:       7403
 
PciI A'CATG_T

Cuts at:   6555    6555 
   Size:       7403
 
PflMI CCAn_nnn'nTGG

Cuts at:    760    3321     760 
   Size:       2561    4842
 
PfoI T'CCnGG_A

Cuts at:   1328    1526    2200    3031    3209    1328 
   Size:        198     674     831     178    5522

  Fragments arranged by size: 

                5522     831     674     198     178
 
PmeI GTTT'AAAC

Cuts at:   4402    4402 
   Size:       7403
 
PmlI CAC'GTG

Cuts at:   1458    1458 
   Size:       7403
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5849    5881    5849 
   Size:         32    7371
 
PpuMI rG'GwC_Cy

Cuts at:   3126    3387    3126 
   Size:        261    7142
 
PshAI GACnn'nnGTC

Cuts at:    370     370 
   Size:       7403
 
PspXI vC'TCGA_Gb

Cuts at:   4344    4344 
   Size:       7403
 
PstI C_TGCA'G

Cuts at:   1190    2162    3074    3356    4450    5135    1190 
   Size:        972     912     282    1094     685    3458

  Fragments arranged by size: 

                3458    1094     972     912     685     282
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7403
 
PvuII CAG'CTG

Cuts at:   2655    3060    3102    3831    4828    5188    2655 
   Size:        405      42     729     997     360    4870

  Fragments arranged by size: 

                4870     997     729     405     360      42
 
RsrII CG'GwC_CG

Cuts at:   5598    5598 
   Size:       7403
 
SacI G_AGCT'C

Cuts at:   4417    4417 
   Size:       7403
 
SacII CC_GC'GG

Cuts at:   3705    3705 
   Size:       7403
 
SalI G'TCGA_C

Cuts at:   4440    4440 
   Size:       7403
 
SapI GCTCTTCn'nnn_

Cuts at:   2630    5426    5636    2630 
   Size:       2796     210    4397

  Fragments arranged by size: 

                4397    2796     210
 
SbfI CC_TGCA'GG

Cuts at:   4450    4450 
   Size:       7403
 
ScaI AGT'ACT

Cuts at:   6920    6920 
   Size:       7403
 
SfcI C'TryA_G

Cuts at:     33    1186    1291    2158    3070    3352    4446    5131    6099 
   Size:       1153     105     867     912     282    1094     685     968

Cuts at:   6099    6290      33 
   Size:        191    1146

  Fragments arranged by size: 

                1153    1146    1094     968     912     867     685     282
                 191     105
 
SfoI GGC'GCC

Cuts at:   4835    5083    4835 
   Size:        248    7155
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7403
 
SgrAI Cr'CCGG_yG

Cuts at:    151     151 
   Size:       7403
 
SmaI CCC'GGG

Cuts at:   3385    4396    4425    3385 
   Size:       1011      29    6363

  Fragments arranged by size: 

                6363    1011      29
 
SmlI C'TyrA_G

Cuts at:    526     694    1314    2176    2227    4344    4558    5473    6187 
   Size:        168     620     862      51    2117     214     915     714

Cuts at:   6187    6449     526 
   Size:        262    1480

  Fragments arranged by size: 

                2117    1480     915     862     714     620     262     214
                 168      51
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7403
 
SphI G_CATG'C

Cuts at:   4456    4956    4456 
   Size:        500    6903
 
SspI AAT'ATT

Cuts at:   1281    1281 
   Size:       7403
 
StuI AGG'CCT

Cuts at:   1184    4112    4336    1184 
   Size:       2928     224    4251

  Fragments arranged by size: 

                4251    2928     224
 
StyI C'CwwG_G

Cuts at:   1803    2486    3368    3404    3920    4157    4537    4891    1803 
   Size:        683     882      36     516     237     380     354    4315

  Fragments arranged by size: 

                4315     882     683     516     380     354     237      36
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2782    2967    3606    4986    5119    5578    5787    2782 
   Size:        185     639    1380     133     459     209    4398

  Fragments arranged by size: 

                4398    1380     639     459     209     185     133
 
TatI w'GTAC_w

Cuts at:   4133    6918    4133 
   Size:       2785    4618
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2182    2214    2546    2578    3736    3768    6920    6952    2182 
   Size:         32     332      32    1158      32    3152      32    2633

  Fragments arranged by size: 

                3152    2633    1158     332      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    255    1174    1769    1981    5200     255 
   Size:        919     595     212    3219    2458

  Fragments arranged by size: 

                3219    2458     919     595     212
 
XbaI T'CTAG_A

Cuts at:   2631    4434    2631 
   Size:       1803    5600
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2424    3996    4898    2424 
   Size:       1572     902    4929

  Fragments arranged by size: 

                4929    1572     902
 
XhoI C'TCGA_G

Cuts at:   1314    4344    1314 
   Size:       3030    4373
 
XmaI C'CCGG_G

Cuts at:   3383    4394    4423    3383 
   Size:       1011      29    6363

  Fragments arranged by size: 

                6363    1011      29
 
XmnI GAAnn'nnTTC

Cuts at:   3191    3191 
   Size:       7403
 
ZraI GAC'GTC

Cuts at:    372    3414    3975     372 
   Size:       3042     561    3800

  Fragments arranged by size: 

                3800    3042     561
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AgeI     AloI     ApaI     AscI    AvrII     BaeI     BglI
     BmtI    BsiWI     BsmI    BspEI    BsrGI   BssHII   BstEII  BstZ17I
    CspCI   DraIII     FseI     MfeI     NdeI     NheI     NotI     NruI
     PacI     PsiI   PspOMI     PsrI    SanDI    SexAI     SfiI    SnaBI
     SrfI     SwaI
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