Restriction map for
pF1KB9259
(Circular) (Six-base) MAPSORT of: pF1KB9259.seq Check: 7671 from: 1 to: 6050
pF1KB9259 6050 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1203 2010 2079 1203
Size: 807 69 5174
Fragments arranged by size:
5174 807 69
AccI GT'mk_AC
Cuts at: 3088 3088
Size: 6050
Acc65I G'GTAC_C
Cuts at: 3066 3066
Size: 6050
AclI AA'CG_TT
Cuts at: 5890 5890
Size: 6050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 1701 3875 4307 4660 5322 305
Size: 1396 2174 432 353 662 1033
Fragments arranged by size:
2174 1396 1033 662 432 353
AfeI AGC'GCT
Cuts at: 2125 2899 2125
Size: 774 5276
AflIII A'CryG_T
Cuts at: 3402 5202 5483 3402
Size: 1800 281 3969
Fragments arranged by size:
3969 1800 281
AgeI A'CCGG_T
Cuts at: 1246 1246
Size: 6050
AhdI GACnn_n'nnGTC
Cuts at: 1084 1084
Size: 6050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2493 2493
Size: 6050
AlwNI CAG_nnn'CTG
Cuts at: 1518 2721 3355 4793 1518
Size: 1203 634 1438 2775
Fragments arranged by size:
2775 1438 1203 634
ApaI G_GGCC'C
Cuts at: 187 201 293 400 557 701 1831 2267 2274
Size: 14 92 107 157 144 1130 436 7
Cuts at: 2274 187
Size: 3963
Fragments arranged by size:
3963 1130 436 157 144 107 92 14
7
ApaLI G'TGCA_C
Cuts at: 4888 4888
Size: 6050
ApoI r'AATT_y
Cuts at: 3054 5403 5659 3054
Size: 2349 256 3445
Fragments arranged by size:
3445 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 255 556 747 1020 1140 1913 2267 2584 3025
Size: 301 191 273 120 773 354 317 441
Cuts at: 3025 3070 255
Size: 45 3235
Fragments arranged by size:
3235 773 441 354 317 301 273 191
120 45
BamHI G'GATC_C
Cuts at: 1439 2685 3075 1439
Size: 1246 390 4414
Fragments arranged by size:
4414 1246 390
BanI G'GyrC_C
Cuts at: 445 620 672 863 1215 1899 1905 2543 3066
Size: 175 52 191 352 684 6 638 523
Cuts at: 3066 3480 3728 3763 445
Size: 414 248 35 2732
Fragments arranged by size:
2732 684 638 523 414 352 248 191
175 52 35 6
BanII G_rGCy'C
Cuts at: 187 201 217 242 293 400 557 593 607
Size: 14 16 25 51 107 157 36 14
Cuts at: 607 701 754 1166 1456 1831 2255 2267 2274
Size: 94 53 412 290 375 424 12 7
Cuts at: 2274 3064 4094 187
Size: 790 1030 2143
Fragments arranged by size:
2143 1030 790 424 412 375 290 157
107 94 53 51 36 25 16 14
14 12 7
BbeI G_GCGC'C
Cuts at: 676 1903 3484 3732 676
Size: 1227 1581 248 2994
Fragments arranged by size:
2994 1581 1227 248
BbsI GAAGACnn'nnnn_
Cuts at: 422 2210 2280 422
Size: 1788 70 4192
Fragments arranged by size:
4192 1788 70
BbvCI CC'TCA_GC
Cuts at: 707 707
Size: 6050
BciVI GTATCCnnnnn_n'
Cuts at: 1129 3948 5004 6050 1129
Size: 2819 1056 1046 1129
Fragments arranged by size:
2819 1129 1056 1046
BclI T'GATC_A
Cuts at: 3570 3570
Size: 6050
BglI GCCn_nnn'nGGC
Cuts at: 1630 2180 2194 1630
Size: 550 14 5486
Fragments arranged by size:
5486 550 14
BglII A'GATC_T
Cuts at: 3014 3565 5602 3014
Size: 551 2037 3462
Fragments arranged by size:
3462 2037 551
BlpI GC'TnA_GC
Cuts at: 1495 3162 1495
Size: 1667 4383
Bme1580I G_kGCm'C
Cuts at: 187 201 255 293 400 450 557 580 701
Size: 14 54 38 107 50 107 23 121
Cuts at: 701 1831 2267 2274 2708 3675 3768 4892 187
Size: 1130 436 7 434 967 93 1124 1345
Fragments arranged by size:
1345 1130 1124 967 436 434 121 107
107 93 54 50 38 23 14 7
BmgBI CAC'GTC
Cuts at: 180 180
Size: 6050
BmrI ACTGGGnnnn_n'
Cuts at: 1239 3262 3343 3678 5822 1239
Size: 2023 81 335 2144 1467
Fragments arranged by size:
2144 2023 1467 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 2049 2091 4417 246
Size: 1803 42 2326 1879
Fragments arranged by size:
2326 1879 1803 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 688 746 776 994 1380 1540 1573 1912
Size: 487 58 30 218 386 160 33 339
Cuts at: 1912 2182 2188 2554 2617 2800 2839 201
Size: 270 6 366 63 183 39 3412
Fragments arranged by size:
3412 487 386 366 339 270 218 183
160 63 58 39 33 30 6
Bpu10I CC'TnA_GC
Cuts at: 295 707 295
Size: 412 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1023 1628 2755 2779 3226 4105 4819 5117 1023
Size: 605 1127 24 447 879 714 298 1956
Fragments arranged by size:
1956 1127 879 714 605 447 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 1171 5754 1171
Size: 4583 1467
BsaAI yAC'GTr
Cuts at: 2446 3403 2446
Size: 957 5093
BsaBI GATnn'nnATC
Cuts at: 6 3019 3564 5601 6
Size: 3013 545 2037 455
Fragments arranged by size:
3013 2037 545 455
BsaHI Gr'CG_yC
Cuts at: 673 1571 1900 3481 3729 4431 5927 673
Size: 898 329 1581 248 702 1496 796
Fragments arranged by size:
1581 1496 898 796 702 329 248
BsaWI w'CCGG_w
Cuts at: 1173 1246 3239 3760 4438 4849 4996 5270 1173
Size: 73 1993 521 678 411 147 274 1953
Fragments arranged by size:
1993 1953 678 521 411 274 147 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1513 1540 1543 1570 2435 2465 2527 2557 2618
Size: 27 3 27 865 30 62 30 61
Cuts at: 2618 2648 1513
Size: 30 4915
Fragments arranged by size:
4915 865 62 61 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 743 843 1333 1383 1657 1695 1981 2602 2845
Size: 100 490 50 274 38 286 621 243
Cuts at: 2845 743
Size: 3948
Fragments arranged by size:
3948 621 490 286 274 243 100 50
38
BseYI C'CCAG_C
Cuts at: 147 210 2255 3475 4233 4898 5306 147
Size: 63 2045 1220 758 665 408 891
Fragments arranged by size:
2045 1220 891 758 665 408 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1035 2038 2107 2170 658
Size: 377 1003 69 63 4538
Fragments arranged by size:
4538 1003 377 69 63
BsiEI CG_ry'CG
Cuts at: 104 129 1823 2005 2415 3592 3638 4868 104
Size: 25 1694 182 410 1177 46 1230 1286
Fragments arranged by size:
1694 1286 1230 1177 410 182 46 25
BsiHKAI G_wGCw'C
Cuts at: 1456 2540 3064 3842 4032 4892 1456
Size: 1084 524 778 190 860 2614
Fragments arranged by size:
2614 1084 860 778 524 190
BsiWI C'GTAC_G
Cuts at: 2345 2345
Size: 6050
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2020
Size: 6050
Bsp1286I G_dGCh'C
Cuts at: 187 201 217 242 255 293 400 450 557
Size: 14 16 25 13 38 107 50 107
Cuts at: 557 580 593 607 701 754 1166 1456 1831
Size: 23 13 14 94 53 412 290 375
Cuts at: 1831 2255 2267 2274 2540 2708 3064 3675 3768
Size: 424 12 7 266 168 356 611 93
Cuts at: 3768 3842 4032 4094 4892 187
Size: 74 190 62 798 1345
Fragments arranged by size:
1345 798 611 424 412 375 356 290
266 190 168 107 107 94 93 74
62 53 50 38 25 23 16 14
14 13 13 12 7
BspEI T'CCGG_A
Cuts at: 1173 1173
Size: 6050
BspHI T'CATG_A
Cuts at: 4482 4482
Size: 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 1203 1589 2010 2079 3100 3314 3616 3997
Size: 988 386 421 69 1021 214 302 381
Cuts at: 3997 215
Size: 2268
Fragments arranged by size:
2268 1021 988 421 386 381 302 214
69
BsrBI CCG'CTC
Cuts at: 50 1289 1325 4342 4396 5880 6045 50
Size: 1239 36 3017 54 1484 165 55
Fragments arranged by size:
3017 1484 1239 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 3962 3962
Size: 6050
BsrFI r'CCGG_y
Cuts at: 1246 1633 1902 2355 2410 2886 2918 4048 4229
Size: 387 269 453 55 476 32 1130 181
Cuts at: 4229 1246
Size: 3067
Fragments arranged by size:
3067 1130 476 453 387 269 181 55
32
BssHII G'CGCG_C
Cuts at: 1882 2935 2961 1882
Size: 1053 26 4971
Fragments arranged by size:
4971 1053 26
BssSI C'ACGA_G
Cuts at: 1804 4321 5029 1804
Size: 2517 708 2825
Fragments arranged by size:
2825 2517 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3427 3427
Size: 6050
BstBI TT'CG_AA
Cuts at: 4411 4411
Size: 6050
BstEII G'GTnAC_C
Cuts at: 115 1237 115
Size: 1122 4928
BstXI CCAn_nnnn'nTGG
Cuts at: 1071 2080 1071
Size: 1009 5041
BstYI r'GATC_y
Cuts at: 1 1265 1439 2591 2603 2685 3014 3075 3542
Size: 1264 174 1152 12 82 329 61 467
Cuts at: 3542 3565 3900 4146 4452 4464 4550 5602 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1264 1152 1052 467 449 335 329 306
246 174 86 82 61 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 326 2825 326
Size: 2499 3551
BtgI C'CryG_G
Cuts at: 120 1307 1565 1874 2189 2912 120
Size: 1187 258 309 315 723 3258
Fragments arranged by size:
3258 1187 723 315 309 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1514 4000 4181 4344 1514
Size: 2486 181 163 3220
Fragments arranged by size:
3220 2486 181 163
BtsI GCAGTG_nn'
Cuts at: 578 603 2338 2616 2690 3293 578
Size: 25 1735 278 74 603 3335
Fragments arranged by size:
3335 1735 603 278 74 25
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 3756 5100 3756
Size: 1344 4706
EaeI y'GGCC_r
Cuts at: 390 1820 2002 2186 2195 2357 2412 2954 2987
Size: 1430 182 184 9 162 55 542 33
Cuts at: 2987 3635 3809 4200 4227 5572 390
Size: 648 174 391 27 1345 868
Fragments arranged by size:
1430 1345 868 648 542 391 184 182
174 162 55 33 27 9
EagI C'GGCC_G
Cuts at: 1820 2002 2412 3635 1820
Size: 182 410 1223 4235
Fragments arranged by size:
4235 1223 410 182
EarI CTCTTCn'nnn_
Cuts at: 708 2090 4073 4283 708
Size: 1382 1983 210 2475
Fragments arranged by size:
2475 1983 1382 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1839 2581 2837 2965 4258 5000 5146 5860 1839
Size: 742 256 128 1293 742 146 714 2029
Fragments arranged by size:
2029 1293 742 742 714 256 146 128
EcoICRI GAG'CTC
Cuts at: 1454 3062 1454
Size: 1608 4442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 305 688 746 776 994 1380 1540 1573
Size: 104 383 58 30 218 386 160 33
Cuts at: 1573 1701 1912 2182 2188 2554 2617 2800 2839
Size: 128 211 270 6 366 63 183 39
Cuts at: 2839 3875 4307 4660 5322 201
Size: 1036 432 353 662 929
Fragments arranged by size:
1036 929 662 432 386 383 366 353
270 218 211 183 160 128 104 63
58 39 33 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 304 329 375 1275 2235 304
Size: 25 46 900 960 4119
Fragments arranged by size:
4119 960 900 46 25
EcoO109I rG'GnC_Cy
Cuts at: 184 197 198 289 290 322 396 397 553
Size: 13 1 91 1 32 74 1 156
Cuts at: 553 760 941 989 1725 1755 1827 1828 2172
Size: 207 181 48 736 30 72 1 344
Cuts at: 2172 2211 2264 2271 2718 2736 2841 3189 184
Size: 39 53 7 447 18 105 348 3045
Fragments arranged by size:
3045 736 447 348 344 207 181 156
105 91 74 72 53 48 39 32
30 18 13 7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 3054 3054
Size: 6050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4069 4069
Size: 6050
FseI GG_CCGG'CC
Cuts at: 2414 2414
Size: 6050
FspI TGC'GCA
Cuts at: 3831 3831
Size: 6050
HaeII r_GCGC'y
Cuts at: 568 676 1020 1842 1903 2127 2901 3002 3484
Size: 108 344 822 61 224 774 101 482
Cuts at: 3484 3732 4962 5733 568
Size: 248 1230 771 885
Fragments arranged by size:
1230 885 822 774 771 482 344 248
224 108 101 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1066 1070 1098 1102 1540 1572 3892
Size: 32 736 4 28 4 438 32 2320
Cuts at: 3892 3922 3924 3954 298
Size: 30 2 30 2394
Fragments arranged by size:
2394 2320 736 438 32 32 30 30
28 4 4 2
HincII GTy'rAC
Cuts at: 3089 3089
Size: 6050
KasI G'GCGC_C
Cuts at: 672 1899 3480 3728 672
Size: 1227 1581 248 2994
Fragments arranged by size:
2994 1581 1227 248
KpnI G_GTAC'C
Cuts at: 3070 3070
Size: 6050
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 4809 4993 1010
Size: 3799 184 2067
Fragments arranged by size:
3799 2067 184
MscI TGG'CCA
Cuts at: 392 2188 2197 3811 392
Size: 1796 9 1614 2631
Fragments arranged by size:
2631 1796 1614 9
MslI CAynn'nnrTG
Cuts at: 1057 1063 2014 2506 4039 4166 1057
Size: 6 951 492 1533 127 2941
Fragments arranged by size:
2941 1533 951 492 127 6
MspA1I CmG'CkG
Cuts at: 340 1040 1518 1684 1992 2807 2925 3161 3475
Size: 700 478 166 308 815 118 236 314
Cuts at: 3475 3835 4619 4864 340
Size: 360 784 245 1526
Fragments arranged by size:
1526 815 784 700 478 360 314 308
245 236 166 118
NaeI GCC'GGC
Cuts at: 1635 2357 2412 4231 1635
Size: 722 55 1819 3454
Fragments arranged by size:
3454 1819 722 55
NarI GG'CG_CC
Cuts at: 673 1900 3481 3729 673
Size: 1227 1581 248 2994
Fragments arranged by size:
2994 1581 1227 248
NcoI C'CATG_G
Cuts at: 120 1307 1874 120
Size: 1187 567 4296
Fragments arranged by size:
4296 1187 567
NdeI CA'TA_TG
Cuts at: 173 173
Size: 6050
NgoMIV G'CCGG_C
Cuts at: 1633 2355 2410 4229 1633
Size: 722 55 1819 3454
Fragments arranged by size:
3454 1819 722 55
NspI r_CATG'y
Cuts at: 1062 1602 3103 3603 5206 1062
Size: 540 1501 500 1603 1906
Fragments arranged by size:
1906 1603 1501 540 500
PasI CC'CwG_GG
Cuts at: 946 2404 946
Size: 1458 4592
PciI A'CATG_T
Cuts at: 5202 5202
Size: 6050
PflMI CCAn_nnn'nTGG
Cuts at: 666 884 1518 1566 2553 2721 2807 666
Size: 218 634 48 987 168 86 3909
Fragments arranged by size:
3909 987 634 218 168 86 48
PfoI T'CCnGG_A
Cuts at: 470 2425 2594 2714 2777 2816 470
Size: 1955 169 120 63 39 3704
Fragments arranged by size:
3704 1955 169 120 63 39
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 989 2718 2736 2841 989
Size: 1729 18 105 4198
Fragments arranged by size:
4198 1729 105 18
PspOMI G'GGCC_C
Cuts at: 183 197 289 396 553 697 1827 2263 2270
Size: 14 92 107 157 144 1130 436 7
Cuts at: 2270 183
Size: 3963
Fragments arranged by size:
3963 1130 436 157 144 107 92 14
7
PstI C_TGCA'G
Cuts at: 1214 1846 2219 3097 3782 1214
Size: 632 373 878 685 3482
Fragments arranged by size:
3482 878 685 632 373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 1518 2807 3475 3835 1518
Size: 1289 668 360 3733
Fragments arranged by size:
3733 1289 668 360
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 1456 3064 1456
Size: 1608 4442
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 4073 4283 4073
Size: 210 5840
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 5567 5567
Size: 6050
SexAI A'CCwGG_T
Cuts at: 623 623
Size: 6050
SfcI C'TryA_G
Cuts at: 33 1210 1467 1842 2215 2743 2785 3093 3778
Size: 1177 257 375 373 528 42 308 685
Cuts at: 3778 4746 4937 33
Size: 968 191 1146
Fragments arranged by size:
1177 1146 968 685 528 375 373 308
257 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 1630 2180 2194 1630
Size: 550 14 5486
Fragments arranged by size:
5486 550 14
SfoI GGC'GCC
Cuts at: 674 1901 3482 3730 674
Size: 1227 1581 248 2994
Fragments arranged by size:
2994 1581 1227 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SgrAI Cr'CCGG_yG
Cuts at: 1246 1902 1246
Size: 656 5394
SmaI CCC'GGG
Cuts at: 558 749 1142 1915 2269 3072 558
Size: 191 393 773 354 803 3536
Fragments arranged by size:
3536 803 773 393 354 191
SmlI C'TyrA_G
Cuts at: 1002 1643 2770 2794 3205 4120 4834 5096 1002
Size: 641 1127 24 411 915 714 262 1956
Fragments arranged by size:
1956 1127 915 714 641 411 262 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 1602 3103 3603 1602
Size: 1501 500 4049
Fragments arranged by size:
4049 1501 500
SrfI GCCC'GGGC
Cuts at: 558 749 558
Size: 191 5859
StuI AGG'CCT
Cuts at: 226 2183 226
Size: 1957 4093
StyI C'CwwG_G
Cuts at: 120 701 1181 1307 1874 3184 3538 120
Size: 581 480 126 567 1310 354 2632
Fragments arranged by size:
2632 1310 581 567 480 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 143 446 1074 1867 3633 3766 4225 4434 143
Size: 303 628 793 1766 133 459 209 1759
Fragments arranged by size:
1766 1759 793 628 459 303 209 133
TatI w'GTAC_w
Cuts at: 5565 5565
Size: 6050
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 796 828 1424 1456 1513 1545 2435 2467 2527
Size: 32 596 32 57 32 890 32 60
Cuts at: 2527 2559 2618 2650 5567 5599 796
Size: 32 59 32 2917 32 1247
Fragments arranged by size:
2917 1247 890 596 60 59 57 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3847 3847
Size: 6050
XbaI T'CTAG_A
Cuts at: 3081 3081
Size: 6050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1129 3545 1129
Size: 2416 3634
XmaI C'CCGG_G
Cuts at: 556 747 1140 1913 2267 3070 556
Size: 191 393 773 354 803 3536
Fragments arranged by size:
3536 803 773 393 354 191
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsmI BsrGI BstZ17I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SnaBI SspI SwaI XhoI XmnI ZraI