Restriction map for
pF1KB9261
(Circular) (Six-base) MAPSORT of: pF1KB9261.seq Check: 7440 from: 1 to: 3725
pF1KB9261 3725 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 1550 1982 2729 2997 531
Size: 1019 432 747 268 1259
Fragments arranged by size:
1259 1019 747 432 268
AfeI AGC'GCT
Cuts at: 131 691 131
Size: 560 3165
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 1030 2597 1030
Size: 1567 2158
ApaI G_GGCC'C
Cuts at: 117 117
Size: 3725
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 729 3078 3334 729
Size: 2349 256 1120
Fragments arranged by size:
2349 1120 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 497 745 497
Size: 248 3477
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 136 175 520 566 741 1155 1403 1438 136
Size: 39 345 46 175 414 248 35 2423
Fragments arranged by size:
2423 414 345 248 175 46 39 35
BanII G_rGCy'C
Cuts at: 117 739 1769 117
Size: 622 1030 2073
Fragments arranged by size:
2073 1030 622
BbeI G_GCGC'C
Cuts at: 140 179 1159 1407 140
Size: 39 980 248 2458
Fragments arranged by size:
2458 980 248 39
BbsI GAAGACnn'nnnn_
Cuts at: 425 425
Size: 3725
BbvCI CC'TCA_GC
Cuts at: 682 682
Size: 3725
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 837 837
Size: 3725
Bme1580I G_kGCm'C
Cuts at: 117 1350 1443 2499 117
Size: 1233 93 1056 1343
Fragments arranged by size:
1343 1233 1056 93
BmgBI CAC'GTC
Cuts at: 216 279 216
Size: 63 3662
BmrI ACTGGGnnnn_n'
Cuts at: 937 1018 1353 3497 937
Size: 81 335 2144 1165
Fragments arranged by size:
2144 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 3725
Bpu10I CC'TnA_GC
Cuts at: 682 682
Size: 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 137 176 1156 1404 2106 3602 137
Size: 39 980 248 702 1496 260
Fragments arranged by size:
1496 980 702 260 248 39
BsaWI w'CCGG_w
Cuts at: 914 1435 2113 2387 2534 2945 914
Size: 521 678 274 147 411 1694
Fragments arranged by size:
1694 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 322 352 202
Size: 30 90 30 3575
Fragments arranged by size:
3575 90 30 30
BseYI C'CCAG_C
Cuts at: 132 1150 1908 2485 2981 132
Size: 1018 758 577 496 876
Fragments arranged by size:
1018 876 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 616
Size: 3725
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 739 1517 1707 2499 739
Size: 778 190 792 1965
Fragments arranged by size:
1965 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 222 222
Size: 3725
Bsp1286I G_dGCh'C
Cuts at: 117 739 1350 1443 1517 1707 1769 2499 117
Size: 622 611 93 74 190 62 730 1343
Fragments arranged by size:
1343 730 622 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 596 775 989 1291 1672 596
Size: 179 214 302 381 2649
Fragments arranged by size:
2649 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 120 2017 2071 3555 3720 50
Size: 70 1897 54 1484 165 55
Fragments arranged by size:
1897 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 190 1723 1904 2113 190
Size: 1533 181 209 1802
Fragments arranged by size:
1802 1533 209 181
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstYI r'GATC_y
Cuts at: 1 332 750 1217 1240 1575 1821 2833 2919
Size: 331 418 467 23 335 246 1012 86
Cuts at: 2919 2931 3277 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 418 346 335 331 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 300 509 107
Size: 193 209 3323
Fragments arranged by size:
3323 209 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 214 1431 2289 214
Size: 1217 858 1650
Fragments arranged by size:
1650 1217 858
EaeI y'GGCC_r
Cuts at: 192 222 1310 1484 1875 1902 3247 192
Size: 30 1088 174 391 27 1345 670
Fragments arranged by size:
1345 1088 670 391 174 30 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 531 1550 1982 2729 2997 349
Size: 182 1019 432 747 268 1077
Fragments arranged by size:
1077 1019 747 432 268 182
EcoO109I rG'GnC_Cy
Cuts at: 113 114 864 113
Size: 1 750 2974
Fragments arranged by size:
2974 750 1
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 133 140 179 693 1159 1407 2429 3408 133
Size: 7 39 514 466 248 1022 979 450
Fragments arranged by size:
1022 979 514 466 450 248 39 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1597 1599 1629 1567
Size: 30 2 30 3663
Fragments arranged by size:
3663 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
KasI G'GCGC_C
Cuts at: 136 175 1155 1403 136
Size: 39 980 248 2458
Fragments arranged by size:
2458 980 248 39
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2580 2396
Size: 184 3541
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 302 308 836 1150 1510 2523 2768 302
Size: 6 528 314 360 1013 245 1259
Fragments arranged by size:
1259 1013 528 360 314 245 6
NaeI GCC'GGC
Cuts at: 192 1906 192
Size: 1714 2011
NarI GG'CG_CC
Cuts at: 137 176 1156 1404 137
Size: 39 980 248 2458
Fragments arranged by size:
2458 980 248 39
NcoI C'CATG_G
Cuts at: 107 107
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 190 1904 190
Size: 1714 2011
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PasI CC'CwG_GG
Cuts at: 203 203
Size: 3725
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PflMI CCAn_nnn'nTGG
Cuts at: 150 150
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PspOMI G'GGCC_C
Cuts at: 113 113
Size: 3725
PstI C_TGCA'G
Cuts at: 715 772 1457 715
Size: 57 685 2983
Fragments arranged by size:
2983 685 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 1150 1510 1150
Size: 360 3365
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SacII CC_GC'GG
Cuts at: 303 303
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 711 768 1453 2446 2637 33
Size: 678 57 685 993 191 1121
Fragments arranged by size:
1121 993 685 678 191 57
SfoI GGC'GCC
Cuts at: 138 177 1157 1405 138
Size: 39 980 248 2458
Fragments arranged by size:
2458 980 248 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 499 747 499
Size: 248 3477
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StuI AGG'CCT
Cuts at: 583 709 583
Size: 126 3599
StyI C'CwwG_G
Cuts at: 107 859 1213 107
Size: 752 354 2619
Fragments arranged by size:
2619 752 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 1308 1441 1900 2109 385
Size: 923 133 459 209 2001
Fragments arranged by size:
2001 923 459 209 133
TatI w'GTAC_w
Cuts at: 3240 3240
Size: 3725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 322 354 3242 3274 322
Size: 32 2888 32 773
Fragments arranged by size:
2888 773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 1220 447
Size: 773 2952
XmaI C'CCGG_G
Cuts at: 497 745 497
Size: 248 3477
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BseRI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI