Restriction map for
pF1KB9267
(Circular) (Six-base) MAPSORT of: pF1KB9267.seq Check: 1672 from: 1 to: 3524
pF1KB9267 3524 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 562 562
Size: 3524
Acc65I G'GTAC_C
Cuts at: 540 540
Size: 3524
AclI AA'CG_TT
Cuts at: 3364 3364
Size: 3524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 316 1349 1781 2134 2796 262
Size: 54 1033 432 353 662 990
Fragments arranged by size:
1033 990 662 432 353 54
AflIII A'CryG_T
Cuts at: 876 2676 2957 876
Size: 1800 281 1443
Fragments arranged by size:
1800 1443 281
AlwNI CAG_nnn'CTG
Cuts at: 829 2267 829
Size: 1438 2086
ApaLI G'TGCA_C
Cuts at: 2362 2362
Size: 3524
ApoI r'AATT_y
Cuts at: 251 528 2877 3133 251
Size: 277 2349 256 642
Fragments arranged by size:
2349 642 277 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3524
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3524
AvaI C'yCGr_G
Cuts at: 481 544 481
Size: 63 3461
BamHI G'GATC_C
Cuts at: 549 549
Size: 3524
BanI G'GyrC_C
Cuts at: 540 954 1202 1237 540
Size: 414 248 35 2827
Fragments arranged by size:
2827 414 248 35
BanII G_rGCy'C
Cuts at: 538 1568 538
Size: 1030 2494
BbeI G_GCGC'C
Cuts at: 958 1206 958
Size: 248 3276
BbvCI CC'TCA_GC
Cuts at: 160 160
Size: 3524
BciVI GTATCCnnnnn_n'
Cuts at: 1422 2478 3524 1422
Size: 1056 1046 1422
Fragments arranged by size:
1422 1056 1046
BclI T'GATC_A
Cuts at: 1044 1044
Size: 3524
BglII A'GATC_T
Cuts at: 1039 3076 1039
Size: 2037 1487
BlpI GC'TnA_GC
Cuts at: 636 636
Size: 3524
Bme1580I G_kGCm'C
Cuts at: 276 1149 1242 2366 276
Size: 873 93 1124 1434
Fragments arranged by size:
1434 1124 873 93
BmrI ACTGGGnnnn_n'
Cuts at: 736 817 1152 3296 736
Size: 81 335 2144 964
Fragments arranged by size:
2144 964 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 3524
Bpu10I CC'TnA_GC
Cuts at: 160 184 160
Size: 24 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1579 2293 2591 700
Size: 879 714 298 1633
Fragments arranged by size:
1633 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3228 3228
Size: 3524
BsaAI yAC'GTr
Cuts at: 877 877
Size: 3524
BsaBI GATnn'nnATC
Cuts at: 6 1038 3075 6
Size: 1032 2037 455
Fragments arranged by size:
2037 1032 455
BsaHI Gr'CG_yC
Cuts at: 955 1203 1905 3401 955
Size: 248 702 1496 1078
Fragments arranged by size:
1496 1078 702 248
BsaWI w'CCGG_w
Cuts at: 713 1234 1912 2323 2470 2744 713
Size: 521 678 411 147 274 1493
Fragments arranged by size:
1493 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 949 1707 2372 2780 949
Size: 758 665 408 1693
Fragments arranged by size:
1693 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1066 1112 2342 104
Size: 962 46 1230 1286
Fragments arranged by size:
1286 1230 962 46
BsiHKAI G_wGCw'C
Cuts at: 538 1316 1506 2366 538
Size: 778 190 860 1696
Fragments arranged by size:
1696 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 276 538 1149 1242 1316 1506 1568 2366 276
Size: 262 611 93 74 190 62 798 1434
Fragments arranged by size:
1434 798 611 262 190 93 74 62
BspHI T'CATG_A
Cuts at: 1956 1956
Size: 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 574 788 1090 1471 574
Size: 214 302 381 2627
Fragments arranged by size:
2627 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1816 1870 3354 3519 50
Size: 1766 54 1484 165 55
Fragments arranged by size:
1766 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1436 1436
Size: 3524
BsrFI r'CCGG_y
Cuts at: 1522 1703 1522
Size: 181 3343
BssSI C'ACGA_G
Cuts at: 139 1795 2503 139
Size: 1656 708 1160
Fragments arranged by size:
1656 1160 708
BstAPI GCAn_nnn'nTGC
Cuts at: 901 901
Size: 3524
BstBI TT'CG_AA
Cuts at: 212 1885 212
Size: 1673 1851
BstXI CCAn_nnnn'nTGG
Cuts at: 123 123
Size: 3524
BstYI r'GATC_y
Cuts at: 1 549 1016 1039 1374 1620 1926 1938 2024
Size: 548 467 23 335 246 306 12 86
Cuts at: 2024 3076 1
Size: 1052 449
Fragments arranged by size:
1052 548 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 1655 1818 1474
Size: 181 163 3180
Fragments arranged by size:
3180 181 163
BtsI GCAGTG_nn'
Cuts at: 767 767
Size: 3524
DraI TTT'AAA
Cuts at: 368 523 368
Size: 155 3369
DrdI GACnn_nn'nnGTC
Cuts at: 1230 2574 1230
Size: 1344 2180
EaeI y'GGCC_r
Cuts at: 1109 1283 1674 1701 3046 1109
Size: 174 391 27 1345 1587
Fragments arranged by size:
1587 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1109 1109
Size: 3524
EarI CTCTTCn'nnn_
Cuts at: 431 1547 1757 431
Size: 1116 210 2198
Fragments arranged by size:
2198 1116 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1732 2474 2620 3334 1732
Size: 742 146 714 1922
Fragments arranged by size:
1922 742 714 146
EcoICRI GAG'CTC
Cuts at: 536 536
Size: 3524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 316 376 1349 1781 2134 2796 262
Size: 54 60 973 432 353 662 990
Fragments arranged by size:
990 973 662 432 353 60 54
EcoO109I rG'GnC_Cy
Cuts at: 663 663
Size: 3524
EcoRI G'AATT_C
Cuts at: 528 528
Size: 3524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1543 1543
Size: 3524
FspI TGC'GCA
Cuts at: 1305 1305
Size: 3524
HaeII r_GCGC'y
Cuts at: 515 958 1206 2436 3207 515
Size: 443 248 1230 771 832
Fragments arranged by size:
1230 832 771 443 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 221 253 1366 1396 1398 1428 221
Size: 32 1113 30 2 30 2317
Fragments arranged by size:
2317 1113 32 30 30 2
HincII GTy'rAC
Cuts at: 563 563
Size: 3524
HindIII A'AGCT_T
Cuts at: 362 362
Size: 3524
KasI G'GCGC_C
Cuts at: 954 1202 954
Size: 248 3276
KpnI G_GTAC'C
Cuts at: 544 544
Size: 3524
MluI A'CGCG_T
Cuts at: 2957 2957
Size: 3524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2283 2467 2283
Size: 184 3340
MscI TGG'CCA
Cuts at: 1285 1285
Size: 3524
MslI CAynn'nnrTG
Cuts at: 1513 1640 1513
Size: 127 3397
MspA1I CmG'CkG
Cuts at: 635 949 1309 2093 2338 635
Size: 314 360 784 245 1821
Fragments arranged by size:
1821 784 360 314 245
NaeI GCC'GGC
Cuts at: 1705 1705
Size: 3524
NarI GG'CG_CC
Cuts at: 955 1203 955
Size: 248 3276
NgoMIV G'CCGG_C
Cuts at: 1703 1703
Size: 3524
NspI r_CATG'y
Cuts at: 577 1077 2680 577
Size: 500 1603 1421
Fragments arranged by size:
1603 1421 500
PciI A'CATG_T
Cuts at: 2676 2676
Size: 3524
PmeI GTTT'AAAC
Cuts at: 523 523
Size: 3524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1970 2002 1970
Size: 32 3492
PstI C_TGCA'G
Cuts at: 571 1256 571
Size: 685 2839
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3524
PvuII CAG'CTG
Cuts at: 949 1309 949
Size: 360 3164
RsrII CG'GwC_CG
Cuts at: 1719 1719
Size: 3524
SacI G_AGCT'C
Cuts at: 538 538
Size: 3524
SalI G'TCGA_C
Cuts at: 561 561
Size: 3524
SapI GCTCTTCn'nnn_
Cuts at: 1547 1757 1547
Size: 210 3314
SbfI CC_TGCA'GG
Cuts at: 571 571
Size: 3524
ScaI AGT'ACT
Cuts at: 3041 3041
Size: 3524
SfcI C'TryA_G
Cuts at: 33 189 567 1252 2220 2411 33
Size: 156 378 685 968 191 1146
Fragments arranged by size:
1146 968 685 378 191 156
SfoI GGC'GCC
Cuts at: 956 1204 956
Size: 248 3276
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3524
SmaI CCC'GGG
Cuts at: 546 546
Size: 3524
SmlI C'TyrA_G
Cuts at: 481 679 1594 2308 2570 481
Size: 198 915 714 262 1435
Fragments arranged by size:
1435 915 714 262 198
SpeI A'CTAG_T
Cuts at: 67 465 67
Size: 398 3126
SphI G_CATG'C
Cuts at: 577 1077 577
Size: 500 3024
StyI C'CwwG_G
Cuts at: 116 658 1012 116
Size: 542 354 2628
Fragments arranged by size:
2628 542 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1107 1240 1699 1908 1107
Size: 133 459 209 2723
Fragments arranged by size:
2723 459 209 133
TatI w'GTAC_w
Cuts at: 3039 3039
Size: 3524
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3492
Tth111I GACn'n_nGTC
Cuts at: 1321 1321
Size: 3524
XbaI T'CTAG_A
Cuts at: 555 555
Size: 3524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1019 1019
Size: 3524
XhoI C'TCGA_G
Cuts at: 481 481
Size: 3524
XmaI C'CCGG_G
Cuts at: 544 544
Size: 3524
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI
BglI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI