Restriction map for
pF1KB9271
(Circular) (Six-base) MAPSORT of: pF1KB9271.seq Check: 8918 from: 1 to: 4877
pF1KB9271 4877 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 337 349 337
Size: 12 4865
AccI GT'mk_AC
Cuts at: 1915 1915
Size: 4877
Acc65I G'GTAC_C
Cuts at: 1893 1893
Size: 4877
AclI AA'CG_TT
Cuts at: 4717 4717
Size: 4877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1806 2702 3134 3881 4149 1806
Size: 896 432 747 268 2534
Fragments arranged by size:
2534 896 747 432 268
AflII C'TTAA_G
Cuts at: 600 600
Size: 4877
AflIII A'CryG_T
Cuts at: 2229 3333 4310 2229
Size: 1104 977 2796
Fragments arranged by size:
2796 1104 977
AgeI A'CCGG_T
Cuts at: 3265 3265
Size: 4877
AleI CACnn'nnGTG
Cuts at: 1445 1445
Size: 4877
AlwNI CAG_nnn'CTG
Cuts at: 306 2182 3749 306
Size: 1876 1567 1434
Fragments arranged by size:
1876 1567 1434
ApaI G_GGCC'C
Cuts at: 747 747
Size: 4877
ApaLI G'TGCA_C
Cuts at: 3647 3647
Size: 4877
ApoI r'AATT_y
Cuts at: 494 1668 1881 4230 4486 494
Size: 1174 213 2349 256 885
Fragments arranged by size:
2349 1174 885 256 213
AseI AT'TA_AT
Cuts at: 20 20
Size: 4877
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 1025 1897 1025
Size: 872 4005
BamHI G'GATC_C
Cuts at: 1688 1902 1688
Size: 214 4663
BanI G'GyrC_C
Cuts at: 114 179 1893 2307 2555 2590 114
Size: 65 1714 414 248 35 2401
Fragments arranged by size:
2401 1714 414 248 65 35
BanII G_rGCy'C
Cuts at: 747 1891 2921 747
Size: 1144 1030 2703
Fragments arranged by size:
2703 1144 1030
BbeI G_GCGC'C
Cuts at: 118 2311 2559 118
Size: 2193 248 2436
Fragments arranged by size:
2436 2193 248
BbsI GAAGACnn'nnnn_
Cuts at: 149 149
Size: 4877
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3536 4877 2775
Size: 761 1341 2775
Fragments arranged by size:
2775 1341 761
BclI T'GATC_A
Cuts at: 2397 2397
Size: 4877
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4877
BglII A'GATC_T
Cuts at: 2392 4429 2392
Size: 2037 2840
BlpI GC'TnA_GC
Cuts at: 1989 1989
Size: 4877
Bme1580I G_kGCm'C
Cuts at: 182 747 2502 2595 3651 182
Size: 565 1755 93 1056 1408
Fragments arranged by size:
1755 1408 1056 565 93
BmgBI CAC'GTC
Cuts at: 147 147
Size: 4877
BmrI ACTGGGnnnn_n'
Cuts at: 259 866 2089 2170 2505 4649 259
Size: 607 1223 81 335 2144 487
Fragments arranged by size:
2144 1223 607 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 429 669 3244 429
Size: 240 2575 2062
Fragments arranged by size:
2575 2062 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 244 794 1298 1634 1718 1802 185
Size: 59 550 504 336 84 84 3260
Fragments arranged by size:
3260 550 504 336 84 84 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 531 641 2053 2932 3424 3722 429
Size: 102 110 1412 879 492 298 1584
Fragments arranged by size:
1584 1412 879 492 298 110 102
BsaI GGTCTCn'nnnn_
Cuts at: 220 249 4581 220
Size: 29 4332 516
Fragments arranged by size:
4332 516 29
BsaAI yAC'GTr
Cuts at: 2230 2230
Size: 4877
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 2308 2556 3258 4754 115
Size: 2193 248 702 1496 238
Fragments arranged by size:
2193 1496 702 248 238
BsaWI w'CCGG_w
Cuts at: 940 1360 2066 2587 3265 3539 3686 4097 940
Size: 420 706 521 678 274 147 411 1720
Fragments arranged by size:
1720 706 678 521 420 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1060 1090 1060
Size: 30 4847
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1068 1068
Size: 4877
BseYI C'CCAG_C
Cuts at: 2302 3060 3637 4133 2302
Size: 758 577 496 3046
Fragments arranged by size:
3046 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1485 1485
Size: 4877
BsiEI CG_ry'CG
Cuts at: 104 2419 2465 3673 104
Size: 2315 46 1208 1308
Fragments arranged by size:
2315 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 926 1891 2669 2859 3651 926
Size: 965 778 190 792 2152
Fragments arranged by size:
2152 965 792 778 190
BsmI GAATG_Cn'
Cuts at: 979 1720 1804 979
Size: 741 84 4052
Fragments arranged by size:
4052 741 84
Bsp1286I G_dGCh'C
Cuts at: 182 747 926 1891 2502 2595 2669 2859 2921
Size: 565 179 965 611 93 74 190 62
Cuts at: 2921 3651 182
Size: 730 1408
Fragments arranged by size:
1408 965 730 611 565 190 179 93
74 62
BspEI T'CCGG_A
Cuts at: 4097 4097
Size: 4877
BspHI T'CATG_A
Cuts at: 4053 4053
Size: 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 238 337 349 896 1671 1927 2141 2443 2824
Size: 99 12 547 775 256 214 302 381
Cuts at: 2824 238
Size: 2291
Fragments arranged by size:
2291 775 547 381 302 256 214 99
12
BsrBI CCG'CTC
Cuts at: 50 992 1160 3169 3223 4707 4872 50
Size: 942 168 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 942 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 801 1809 2789 801
Size: 1008 980 2889
Fragments arranged by size:
2889 1008 980
BsrFI r'CCGG_y
Cuts at: 117 2875 3056 3265 117
Size: 2758 181 209 1729
Fragments arranged by size:
2758 1729 209 181
BssSI C'ACGA_G
Cuts at: 3148 3506 3148
Size: 358 4519
BstAPI GCAn_nnn'nTGC
Cuts at: 2254 2254
Size: 4877
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstYI r'GATC_y
Cuts at: 1 439 1688 1902 2369 2392 2727 2973 3985
Size: 438 1249 214 467 23 335 246 1012
Cuts at: 3985 4071 4083 4429 1
Size: 86 12 346 449
Fragments arranged by size:
1249 1012 467 449 438 346 335 246
214 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4877
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1309 2827 3008 3171 1309
Size: 1518 181 163 3015
Fragments arranged by size:
3015 1518 181 163
BtsI GCAGTG_nn'
Cuts at: 1473 2120 1473
Size: 647 4230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 783 818 951 986 1035 1070 1371 1406 1791
Size: 35 133 35 49 35 301 35 385
Cuts at: 1791 1826 783
Size: 35 3834
Fragments arranged by size:
3834 385 301 133 49 35 35 35
35 35
DraI TTT'AAA
Cuts at: 1876 1876
Size: 4877
DraIII CAC_nnn'GTG
Cuts at: 1182 1182
Size: 4877
DrdI GACnn_nn'nnGTC
Cuts at: 2583 3441 2583
Size: 858 4019
EaeI y'GGCC_r
Cuts at: 2462 2636 3027 3054 4399 2462
Size: 174 391 27 1345 2940
Fragments arranged by size:
2940 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 313 999 1419 1587 2900 3110 313
Size: 686 420 168 1313 210 2080
Fragments arranged by size:
2080 1313 686 420 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3085 3395 3541 4687 3085
Size: 310 146 1146 3275
Fragments arranged by size:
3275 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1889 1889
Size: 4877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 244 794 1298 1634 1718 1802 1806 2702
Size: 59 550 504 336 84 84 4 896
Cuts at: 2702 3134 3881 4149 185
Size: 432 747 268 913
Fragments arranged by size:
913 896 747 550 504 432 336 268
84 84 59 4
EcoNI CCTnn'n_nnAGG
Cuts at: 208 208
Size: 4877
EcoO109I rG'GnC_Cy
Cuts at: 200 744 1352 1520 2016 200
Size: 544 608 168 496 3061
Fragments arranged by size:
3061 608 544 496 168
EcoRI G'AATT_C
Cuts at: 1881 1881
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4877
FspI TGC'GCA
Cuts at: 345 2658 345
Size: 2313 2564
HaeII r_GCGC'y
Cuts at: 118 2311 2559 3581 4560 118
Size: 2193 248 1022 979 435
Fragments arranged by size:
2193 1022 979 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1008 1040 2719 2749 2751 2781 1008
Size: 32 1679 30 2 30 3104
Fragments arranged by size:
3104 1679 32 30 30 2
HincII GTy'rAC
Cuts at: 1916 1916
Size: 4877
HindIII A'AGCT_T
Cuts at: 1061 1061
Size: 4877
KasI G'GCGC_C
Cuts at: 114 2307 2555 114
Size: 2193 248 2436
Fragments arranged by size:
2436 2193 248
KpnI G_GTAC'C
Cuts at: 1897 1897
Size: 4877
MluI A'CGCG_T
Cuts at: 4310 4310
Size: 4877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1365 3548 3732 1365
Size: 2183 184 2510
Fragments arranged by size:
2510 2183 184
MscI TGG'CCA
Cuts at: 2638 2638
Size: 4877
MslI CAynn'nnrTG
Cuts at: 647 873 1209 1445 2866 2993 647
Size: 226 336 236 1421 127 2531
Fragments arranged by size:
2531 1421 336 236 226 127
MspA1I CmG'CkG
Cuts at: 393 1988 2302 2662 3675 3920 393
Size: 1595 314 360 1013 245 1350
Fragments arranged by size:
1595 1350 1013 360 314 245
NaeI GCC'GGC
Cuts at: 119 3058 119
Size: 2939 1938
NarI GG'CG_CC
Cuts at: 115 2308 2556 115
Size: 2193 248 2436
Fragments arranged by size:
2436 2193 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4877
NdeI CA'TA_TG
Cuts at: 842 1094 1430 842
Size: 252 336 4289
Fragments arranged by size:
4289 336 252
NgoMIV G'CCGG_C
Cuts at: 117 3056 117
Size: 2939 1938
NspI r_CATG'y
Cuts at: 710 1930 2430 3337 710
Size: 1220 500 907 2250
Fragments arranged by size:
2250 1220 907 500
PciI A'CATG_T
Cuts at: 3333 3333
Size: 4877
PfoI T'CCnGG_A
Cuts at: 419 419
Size: 4877
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4845
PpuMI rG'GwC_Cy
Cuts at: 200 1352 1520 200
Size: 1152 168 3557
Fragments arranged by size:
3557 1152 168
PsiI TTA'TAA
Cuts at: 563 563
Size: 4877
PspOMI G'GGCC_C
Cuts at: 743 743
Size: 4877
PstI C_TGCA'G
Cuts at: 235 1924 2609 235
Size: 1689 685 2503
Fragments arranged by size:
2503 1689 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4877
PvuII CAG'CTG
Cuts at: 393 2302 2662 393
Size: 1909 360 2608
Fragments arranged by size:
2608 1909 360
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 1891 1891
Size: 4877
SalI G'TCGA_C
Cuts at: 1914 1914
Size: 4877
SapI GCTCTTCn'nnn_
Cuts at: 999 1419 1587 2900 3110 999
Size: 420 168 1313 210 2766
Fragments arranged by size:
2766 1313 420 210 168
SbfI CC_TGCA'GG
Cuts at: 235 1924 235
Size: 1689 3188
ScaI AGT'ACT
Cuts at: 4394 4394
Size: 4877
SfcI C'TryA_G
Cuts at: 33 231 1071 1575 1920 2605 3598 3789 33
Size: 198 840 504 345 685 993 191 1121
Fragments arranged by size:
1121 993 840 685 504 345 198 191
SfoI GGC'GCC
Cuts at: 116 2309 2557 116
Size: 2193 248 2436
Fragments arranged by size:
2436 2193 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SgrAI Cr'CCGG_yG
Cuts at: 117 117
Size: 4877
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4877
SmlI C'TyrA_G
Cuts at: 444 510 600 656 1025 2032 2947 3439 3701
Size: 66 90 56 369 1007 915 492 262
Cuts at: 3701 444
Size: 1620
Fragments arranged by size:
1620 1007 915 492 369 262 90 66
56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 1930 2430 1930
Size: 500 4377
StuI AGG'CCT
Cuts at: 326 1147 1315 1399 326
Size: 821 168 84 3804
Fragments arranged by size:
3804 821 168 84
StyI C'CwwG_G
Cuts at: 107 1541 2011 2365 107
Size: 1434 470 354 2619
Fragments arranged by size:
2619 1434 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1000 2460 2593 3052 3261 1000
Size: 1460 133 459 209 2616
Fragments arranged by size:
2616 1460 459 209 133
TatI w'GTAC_w
Cuts at: 4392 4392
Size: 4877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1512 1544 4394 4426 1512
Size: 32 2850 32 1963
Fragments arranged by size:
2850 1963 32 32
Tth111I GACn'n_nGTC
Cuts at: 2674 2674
Size: 4877
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2372
Size: 4877
XhoI C'TCGA_G
Cuts at: 1025 1025
Size: 4877
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4877
XmnI GAAnn'nnTTC
Cuts at: 895 1735 895
Size: 840 4037
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI SwaI ZraI