(Circular) (Six-base) MAPSORT of: pF1KB9273.seq Check: 5621 from: 1 to: 4241 pF1KB9273 4241 bp With 182 enzymes: SgfI * July 15, 2010 18:27 .. AccI GT'mk_AC Cuts at: 892 1279 892 Size: 387 3854 Acc65I G'GTAC_C Cuts at: 1257 1257 Size: 4241 AclI AA'CG_TT Cuts at: 4081 4081 Size: 4241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 730 793 2066 2498 3245 3513 730 Size: 63 1273 432 747 268 1458 Fragments arranged by size: 1458 1273 747 432 268 63 AflIII A'CryG_T Cuts at: 971 1593 2697 3674 971 Size: 622 1104 977 1538 Fragments arranged by size: 1538 1104 977 622 AgeI A'CCGG_T Cuts at: 2629 2629 Size: 4241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1061 1143 1061 Size: 82 4159 AlwNI CAG_nnn'CTG Cuts at: 816 1546 3113 816 Size: 730 1567 1944 Fragments arranged by size: 1944 1567 730 ApaLI G'TGCA_C Cuts at: 1036 3011 1036 Size: 1975 2266 ApoI r'AATT_y Cuts at: 424 789 1245 3594 3850 424 Size: 365 456 2349 256 815 Fragments arranged by size: 2349 815 456 365 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4241 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4241 AvaI C'yCGr_G Cuts at: 1261 1261 Size: 4241 BamHI G'GATC_C Cuts at: 1266 1266 Size: 4241 BanI G'GyrC_C Cuts at: 1257 1671 1919 1954 1257 Size: 414 248 35 3544 Fragments arranged by size: 3544 414 248 35 BanII G_rGCy'C Cuts at: 935 1255 2285 935 Size: 320 1030 2891 Fragments arranged by size: 2891 1030 320 BbeI G_GCGC'C Cuts at: 1675 1923 1675 Size: 248 3993 BbsI GAAGACnn'nnnn_ Cuts at: 857 857 Size: 4241 BbvCI CC'TCA_GC Cuts at: 178 1021 178 Size: 843 3398 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4207 BciVI GTATCCnnnnn_n' Cuts at: 2139 2900 4241 2139 Size: 761 1341 2139 Fragments arranged by size: 2139 1341 761 BclI T'GATC_A Cuts at: 1761 1761 Size: 4241 BglI GCCn_nnn'nGGC Cuts at: 112 552 112 Size: 440 3801 BglII A'GATC_T Cuts at: 293 1756 3793 293 Size: 1463 2037 741 Fragments arranged by size: 2037 1463 741 BlpI GC'TnA_GC Cuts at: 1353 1353 Size: 4241 Bme1580I G_kGCm'C Cuts at: 1040 1866 1959 3015 1040 Size: 826 93 1056 2266 Fragments arranged by size: 2266 1056 826 93 BmgBI CAC'GTC Cuts at: 126 972 126 Size: 846 3395 BmrI ACTGGGnnnn_n' Cuts at: 231 1453 1534 1869 4013 231 Size: 1222 81 335 2144 459 Fragments arranged by size: 2144 1222 459 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2608 2608 Size: 4241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 583 1187 583 Size: 604 3637 Bpu10I CC'TnA_GC Cuts at: 178 1021 178 Size: 843 3398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1417 2296 2788 3086 1417 Size: 879 492 298 2572 Fragments arranged by size: 2572 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3945 3945 Size: 4241 BsaAI yAC'GTr Cuts at: 327 942 1594 327 Size: 615 652 2974 Fragments arranged by size: 2974 652 615 BsaBI GATnn'nnATC Cuts at: 6 1755 3792 6 Size: 1749 2037 455 Fragments arranged by size: 2037 1749 455 BsaHI Gr'CG_yC Cuts at: 1672 1920 2622 4118 1672 Size: 248 702 1496 1795 Fragments arranged by size: 1795 1496 702 248 BsaWI w'CCGG_w Cuts at: 1430 1951 2629 2903 3050 3461 1430 Size: 521 678 274 147 411 2210 Fragments arranged by size: 2210 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 586 678 1066 586 Size: 92 388 3761 Fragments arranged by size: 3761 388 92 BseYI C'CCAG_C Cuts at: 999 1180 1223 1666 2424 3001 3497 999 Size: 181 43 443 758 577 496 1743 Fragments arranged by size: 1743 758 577 496 443 181 43 BsiEI CG_ry'CG Cuts at: 104 1783 1829 3037 104 Size: 1679 46 1208 1308 Fragments arranged by size: 1679 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1040 1255 2033 2223 3015 1040 Size: 215 778 190 792 2266 Fragments arranged by size: 2266 792 778 215 190 Bsp1286I G_dGCh'C Cuts at: 935 1040 1255 1866 1959 2033 2223 2285 3015 Size: 105 215 611 93 74 190 62 730 Cuts at: 3015 935 Size: 2161 Fragments arranged by size: 2161 730 611 215 190 105 93 74 62 BspEI T'CCGG_A Cuts at: 3461 3461 Size: 4241 BspHI T'CATG_A Cuts at: 3417 3417 Size: 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1025 1291 1505 1807 2188 1025 Size: 266 214 302 381 3078 Fragments arranged by size: 3078 381 302 266 214 BsrBI CCG'CTC Cuts at: 50 2533 2587 4071 4236 50 Size: 2483 54 1484 165 55 Fragments arranged by size: 2483 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 473 2153 473 Size: 1680 2561 BsrFI r'CCGG_y Cuts at: 2239 2420 2629 2239 Size: 181 209 3851 Fragments arranged by size: 3851 209 181 BsrGI T'GTAC_A Cuts at: 1007 1007 Size: 4241 BssSI C'ACGA_G Cuts at: 2512 2870 2512 Size: 358 3883 BstAPI GCAn_nnn'nTGC Cuts at: 117 1618 117 Size: 1501 2740 BstBI TT'CG_AA Cuts at: 2602 2602 Size: 4241 BstEII G'GTnAC_C Cuts at: 190 190 Size: 4241 BstYI r'GATC_y Cuts at: 1 293 1170 1266 1733 1756 2091 2337 3349 Size: 292 877 96 467 23 335 246 1012 Cuts at: 3349 3435 3447 3793 1 Size: 86 12 346 449 Fragments arranged by size: 1012 877 467 449 346 335 292 246 96 86 23 12 BstZ17I GTA'TAC Cuts at: 893 893 Size: 4241 Bsu36I CC'TnA_GG Cuts at: 661 661 Size: 4241 BtgI C'CryG_G Cuts at: 161 161 Size: 4241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2191 2372 2535 2191 Size: 181 163 3897 Fragments arranged by size: 3897 181 163 BtsI GCAGTG_nn' Cuts at: 1484 1484 Size: 4241 DraI TTT'AAA Cuts at: 618 1240 618 Size: 622 3619 DraIII CAC_nnn'GTG Cuts at: 129 129 Size: 4241 DrdI GACnn_nn'nnGTC Cuts at: 1947 2805 1947 Size: 858 3383 EaeI y'GGCC_r Cuts at: 164 1230 1826 2000 2391 2418 3763 164 Size: 1066 596 174 391 27 1345 642 Fragments arranged by size: 1345 1066 642 596 391 174 27 EagI C'GGCC_G Cuts at: 1826 1826 Size: 4241 EarI CTCTTCn'nnn_ Cuts at: 778 2264 2474 778 Size: 1486 210 2545 Fragments arranged by size: 2545 1486 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 198 1096 2449 2759 2905 4051 198 Size: 898 1353 310 146 1146 388 Fragments arranged by size: 1353 1146 898 388 310 146 EcoICRI GAG'CTC Cuts at: 1253 1253 Size: 4241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 583 730 793 1187 2066 2498 3245 3513 583 Size: 147 63 394 879 432 747 268 1311 Fragments arranged by size: 1311 879 747 432 394 268 147 63 EcoO109I rG'GnC_Cy Cuts at: 1014 1380 1014 Size: 366 3875 EcoRI G'AATT_C Cuts at: 1245 1245 Size: 4241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 4241 FspI TGC'GCA Cuts at: 2022 2022 Size: 4241 HaeII r_GCGC'y Cuts at: 161 1675 1923 2945 3924 161 Size: 1514 248 1022 979 478 Fragments arranged by size: 1514 1022 979 478 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 378 410 2083 2113 2115 2145 378 Size: 32 1673 30 2 30 2474 Fragments arranged by size: 2474 1673 32 30 30 2 HincII GTy'rAC Cuts at: 1280 1280 Size: 4241 KasI G'GCGC_C Cuts at: 1671 1919 1671 Size: 248 3993 KpnI G_GTAC'C Cuts at: 1261 1261 Size: 4241 MluI A'CGCG_T Cuts at: 3674 3674 Size: 4241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2912 3096 2912 Size: 184 4057 MscI TGG'CCA Cuts at: 1232 2002 1232 Size: 770 3471 MslI CAynn'nnrTG Cuts at: 766 2230 2357 766 Size: 1464 127 2650 Fragments arranged by size: 2650 1464 127 MspA1I CmG'CkG Cuts at: 607 1164 1352 1666 2026 3039 3284 607 Size: 557 188 314 360 1013 245 1564 Fragments arranged by size: 1564 1013 557 360 314 245 188 NaeI GCC'GGC Cuts at: 2422 2422 Size: 4241 NarI GG'CG_CC Cuts at: 1672 1920 1672 Size: 248 3993 NgoMIV G'CCGG_C Cuts at: 2420 2420 Size: 4241 NspI r_CATG'y Cuts at: 771 1294 1794 2701 771 Size: 523 500 907 2311 Fragments arranged by size: 2311 907 523 500 PciI A'CATG_T Cuts at: 2697 2697 Size: 4241 PflMI CCAn_nnn'nTGG Cuts at: 1116 1116 Size: 4241 PfoI T'CCnGG_A Cuts at: 1073 1073 Size: 4241 PmeI GTTT'AAAC Cuts at: 1240 1240 Size: 4241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 211 243 3380 3412 211 Size: 32 3137 32 1040 Fragments arranged by size: 3137 1040 32 32 PpuMI rG'GwC_Cy Cuts at: 1014 1014 Size: 4241 PstI C_TGCA'G Cuts at: 821 1288 1973 821 Size: 467 685 3089 Fragments arranged by size: 3089 685 467 PvuI CG_AT'CG Cuts at: 104 104 Size: 4241 PvuII CAG'CTG Cuts at: 607 1666 2026 607 Size: 1059 360 2822 Fragments arranged by size: 2822 1059 360 RsrII CG'GwC_CG Cuts at: 2436 2436 Size: 4241 SacI G_AGCT'C Cuts at: 1255 1255 Size: 4241 SalI G'TCGA_C Cuts at: 1278 1278 Size: 4241 SapI GCTCTTCn'nnn_ Cuts at: 2264 2474 2264 Size: 210 4031 SbfI CC_TGCA'GG Cuts at: 1288 1288 Size: 4241 ScaI AGT'ACT Cuts at: 3758 3758 Size: 4241 SexAI A'CCwGG_T Cuts at: 194 828 194 Size: 634 3607 SfcI C'TryA_G Cuts at: 33 817 1284 1969 2962 3153 33 Size: 784 467 685 993 191 1121 Fragments arranged by size: 1121 993 784 685 467 191 SfoI GGC'GCC Cuts at: 1673 1921 1673 Size: 248 3993 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4241 SmaI CCC'GGG Cuts at: 1263 1263 Size: 4241 SmlI C'TyrA_G Cuts at: 1396 2311 2803 3065 1396 Size: 915 492 262 2572 Fragments arranged by size: 2572 915 492 262 SnaBI TAC'GTA Cuts at: 327 327 Size: 4241 SpeI A'CTAG_T Cuts at: 67 1122 67 Size: 1055 3186 SphI G_CATG'C Cuts at: 1294 1794 1294 Size: 500 3741 StuI AGG'CCT Cuts at: 115 115 Size: 4241 StyI C'CwwG_G Cuts at: 896 1375 1729 896 Size: 479 354 3408 Fragments arranged by size: 3408 479 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1013 1824 1957 2416 2625 1013 Size: 811 133 459 209 2629 Fragments arranged by size: 2629 811 459 209 133 TatI w'GTAC_w Cuts at: 250 1007 3756 250 Size: 757 2749 735 Fragments arranged by size: 2749 757 735 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 826 858 3758 3790 826 Size: 32 2900 32 1277 Fragments arranged by size: 2900 1277 32 32 Tth111I GACn'n_nGTC Cuts at: 830 958 2038 830 Size: 128 1080 3033 Fragments arranged by size: 3033 1080 128 XbaI T'CTAG_A Cuts at: 1272 1272 Size: 4241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 678 1220 1736 678 Size: 542 516 3183 Fragments arranged by size: 3183 542 516 XmaI C'CCGG_G Cuts at: 1261 1261 Size: 4241 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BfrBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstXI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI