Restriction map for
pF1KB9280
(Circular) (Six-base) MAPSORT of: pF1KB9280.seq Check: 4319 from: 1 to: 4850
pF1KB9280 4850 bp
With 182 enzymes: SgfI *
September 22, 2011 10:48 ..
AccI GT'mk_AC
Cuts at: 1888 1888
Size: 4850
Acc65I G'GTAC_C
Cuts at: 1109 1200 1866 1109
Size: 91 666 4093
Fragments arranged by size:
4093 666 91
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3107 3854 4122 2675
Size: 432 747 268 3403
Fragments arranged by size:
3403 747 432 268
AflII C'TTAA_G
Cuts at: 897 897
Size: 4850
AflIII A'CryG_T
Cuts at: 1000 1320 2202 3306 4283 1000
Size: 320 882 1104 977 1567
Fragments arranged by size:
1567 1104 977 882 320
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AhdI GACnn_n'nnGTC
Cuts at: 594 594
Size: 4850
AleI CACnn'nnGTG
Cuts at: 1543 1543
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 207 265 1042 2155 3722 207
Size: 58 777 1113 1567 1335
Fragments arranged by size:
1567 1335 1113 777 58
ApaLI G'TGCA_C
Cuts at: 1324 3620 1324
Size: 2296 2554
ApoI r'AATT_y
Cuts at: 1468 1854 4203 4459 1468
Size: 386 2349 256 1859
Fragments arranged by size:
2349 1859 386 256
AscI GG'CGCG_CC
Cuts at: 480 480
Size: 4850
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 298 319 1870 298
Size: 21 1551 3278
Fragments arranged by size:
3278 1551 21
BamHI G'GATC_C
Cuts at: 1875 1875
Size: 4850
BanI G'GyrC_C
Cuts at: 424 580 1109 1200 1866 2280 2528 2563 424
Size: 156 529 91 666 414 248 35 2711
Fragments arranged by size:
2711 666 529 414 248 156 91 35
BanII G_rGCy'C
Cuts at: 1688 1864 2894 1688
Size: 176 1030 3644
Fragments arranged by size:
3644 1030 176
BbeI G_GCGC'C
Cuts at: 584 2284 2532 584
Size: 1700 248 2902
Fragments arranged by size:
2902 1700 248
BbsI GAAGACnn'nnnn_
Cuts at: 1021 1616 1021
Size: 595 4255
BbvCI CC'TCA_GC
Cuts at: 291 291
Size: 4850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 630 664 630
Size: 34 4816
BciVI GTATCCnnnnn_n'
Cuts at: 1335 2748 3509 4850 1335
Size: 1413 761 1341 1335
Fragments arranged by size:
1413 1341 1335 761
BclI T'GATC_A
Cuts at: 659 1116 2370 659
Size: 457 1254 3139
Fragments arranged by size:
3139 1254 457
BglII A'GATC_T
Cuts at: 2365 4402 2365
Size: 2037 2813
BlpI GC'TnA_GC
Cuts at: 148 508 1772 1962 148
Size: 360 1264 190 3036
Fragments arranged by size:
3036 1264 360 190
Bme1580I G_kGCm'C
Cuts at: 410 1328 2475 2568 3624 410
Size: 918 1147 93 1056 1636
Fragments arranged by size:
1636 1147 1056 918 93
BmgBI CAC'GTC
Cuts at: 1758 1758
Size: 4850
BmrI ACTGGGnnnn_n'
Cuts at: 2062 2143 2478 4622 2062
Size: 81 335 2144 2290
Fragments arranged by size:
2290 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 3217 207
Size: 3010 1840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 741 398
Size: 343 4507
Bpu10I CC'TnA_GC
Cuts at: 291 609 291
Size: 318 4532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 2026 2905 3397 3695 177
Size: 1849 879 492 298 1332
Fragments arranged by size:
1849 1332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 556 4554 556
Size: 3998 852
BsaAI yAC'GTr
Cuts at: 1323 2203 1323
Size: 880 3970
BsaBI GATnn'nnATC
Cuts at: 6 2364 4401 6
Size: 2358 2037 455
Fragments arranged by size:
2358 2037 455
BsaHI Gr'CG_yC
Cuts at: 134 581 595 2281 2529 3231 4727 134
Size: 447 14 1686 248 702 1496 257
Fragments arranged by size:
1686 1496 702 447 257 248 14
BsaWI w'CCGG_w
Cuts at: 2039 2560 3238 3512 3659 4070 2039
Size: 521 678 274 147 411 2819
Fragments arranged by size:
2819 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 316 346 316
Size: 30 4820
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 395 948 245
Size: 150 553 4147
Fragments arranged by size:
4147 553 150
BseYI C'CCAG_C
Cuts at: 468 2275 3033 3610 4106 468
Size: 1807 758 577 496 1212
Fragments arranged by size:
1807 1212 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 145 2392 2438 3646 104
Size: 41 2247 46 1208 1308
Fragments arranged by size:
2247 1308 1208 46 41
BsiHKAI G_wGCw'C
Cuts at: 326 978 1328 1864 2642 2832 3624 326
Size: 652 350 536 778 190 792 1552
Fragments arranged by size:
1552 792 778 652 536 350 190
Bsp1286I G_dGCh'C
Cuts at: 326 410 978 1328 1688 1864 2475 2568 2642
Size: 84 568 350 360 176 611 93 74
Cuts at: 2642 2832 2894 3624 326
Size: 190 62 730 1552
Fragments arranged by size:
1552 730 611 568 360 350 190 176
93 84 74 62
BspEI T'CCGG_A
Cuts at: 4070 4070
Size: 4850
BspHI T'CATG_A
Cuts at: 1334 4026 1334
Size: 2692 2158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 110 1900 2114 2416 2797 110
Size: 1790 214 302 381 2163
Fragments arranged by size:
2163 1790 381 302 214
BsrBI CCG'CTC
Cuts at: 50 147 404 1776 3142 3196 4680 4845 50
Size: 97 257 1372 1366 54 1484 165 55
Fragments arranged by size:
1484 1372 1366 257 165 97 55 54
BsrDI GCAATG_nn'
Cuts at: 2762 2762
Size: 4850
BsrFI r'CCGG_y
Cuts at: 358 476 2848 3029 3238 358
Size: 118 2372 181 209 1970
Fragments arranged by size:
2372 1970 209 181 118
BssHII G'CGCG_C
Cuts at: 480 480
Size: 4850
BssSI C'ACGA_G
Cuts at: 181 3121 3479 181
Size: 2940 358 1552
Fragments arranged by size:
2940 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 207 265 2227 207
Size: 58 1962 2830
Fragments arranged by size:
2830 1962 58
BstBI TT'CG_AA
Cuts at: 491 3211 491
Size: 2720 2130
BstXI CCAn_nnnn'nTGG
Cuts at: 342 342
Size: 4850
BstYI r'GATC_y
Cuts at: 1 1875 2342 2365 2700 2946 3958 4044 4056
Size: 1874 467 23 335 246 1012 86 12
Cuts at: 4056 4402 1
Size: 346 449
Fragments arranged by size:
1874 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1165 2800 2981 3144 1165
Size: 1635 181 163 2871
Fragments arranged by size:
2871 1635 181 163
BtsI GCAGTG_nn'
Cuts at: 426 2093 426
Size: 1667 3183
DraI TTT'AAA
Cuts at: 1849 1849
Size: 4850
DrdI GACnn_nn'nnGTC
Cuts at: 570 1756 2556 3414 570
Size: 1186 800 858 2006
Fragments arranged by size:
2006 1186 858 800
EaeI y'GGCC_r
Cuts at: 142 2435 2609 3000 3027 4372 142
Size: 2293 174 391 27 1345 620
Fragments arranged by size:
2293 1345 620 391 174 27
EagI C'GGCC_G
Cuts at: 142 2435 142
Size: 2293 2557
EarI CTCTTCn'nnn_
Cuts at: 1615 1815 2873 3083 1615
Size: 200 1058 210 3382
Fragments arranged by size:
3382 1058 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 380 3058 3368 3514 4660 380
Size: 2678 310 146 1146 570
Fragments arranged by size:
2678 1146 570 310 146
EcoICRI GAG'CTC
Cuts at: 1862 1862
Size: 4850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 741 2675 3107 3854 4122 398
Size: 343 1934 432 747 268 1126
Fragments arranged by size:
1934 1126 747 432 343 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1791 1791
Size: 4850
EcoO109I rG'GnC_Cy
Cuts at: 1989 1989
Size: 4850
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
EcoRV GAT'ATC
Cuts at: 1163 1163
Size: 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1008 2869 1008
Size: 1861 2989
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 349 584 2284 2532 3554 4533 349
Size: 235 1700 248 1022 979 666
Fragments arranged by size:
1700 1022 979 666 248 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 943 975 1329 1361 2692 2722 2724
Size: 32 331 32 354 32 1331 30 2
Cuts at: 2724 2754 580
Size: 30 2676
Fragments arranged by size:
2676 1331 354 331 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1889 1889
Size: 4850
KasI G'GCGC_C
Cuts at: 580 2280 2528 580
Size: 1700 248 2902
Fragments arranged by size:
2902 1700 248
KpnI G_GTAC'C
Cuts at: 1113 1204 1870 1113
Size: 91 666 4093
Fragments arranged by size:
4093 666 91
MluI A'CGCG_T
Cuts at: 4283 4283
Size: 4850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 359 3521 3705 207
Size: 152 3162 184 1352
Fragments arranged by size:
3162 1352 184 152
MscI TGG'CCA
Cuts at: 2611 2611
Size: 4850
MslI CAynn'nnrTG
Cuts at: 813 1543 2839 2966 813
Size: 730 1296 127 2697
Fragments arranged by size:
2697 1296 730 127
MspA1I CmG'CkG
Cuts at: 116 265 280 862 1350 1375 1961 2275 2635
Size: 149 15 582 488 25 586 314 360
Cuts at: 2635 3648 3893 116
Size: 1013 245 1073
Fragments arranged by size:
1073 1013 586 582 488 360 314 245
149 25 15
NaeI GCC'GGC
Cuts at: 3031 3031
Size: 4850
NarI GG'CG_CC
Cuts at: 581 2281 2529 581
Size: 1700 248 2902
Fragments arranged by size:
2902 1700 248
NgoMIV G'CCGG_C
Cuts at: 3029 3029
Size: 4850
NotI GC'GGCC_GC
Cuts at: 142 142
Size: 4850
NspI r_CATG'y
Cuts at: 537 1903 2403 3310 537
Size: 1366 500 907 2077
Fragments arranged by size:
2077 1366 907 500
PasI CC'CwG_GG
Cuts at: 1681 1681
Size: 4850
PciI A'CATG_T
Cuts at: 3306 3306
Size: 4850
PflMI CCAn_nnn'nTGG
Cuts at: 186 186
Size: 4850
PfoI T'CCnGG_A
Cuts at: 1792 1792
Size: 4850
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PmlI CAC'GTG
Cuts at: 1323 1323
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4818
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 445 477 445
Size: 32 4818
PstI C_TGCA'G
Cuts at: 121 212 261 1897 2582 121
Size: 91 49 1636 685 2389
Fragments arranged by size:
2389 1636 685 91 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 116 1350 1375 2275 2635 116
Size: 1234 25 900 360 2331
Fragments arranged by size:
2331 1234 900 360 25
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 1864 1864
Size: 4850
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 2873 3083 2873
Size: 210 4640
SbfI CC_TGCA'GG
Cuts at: 1897 1897
Size: 4850
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SfcI C'TryA_G
Cuts at: 33 117 208 257 1280 1893 2578 3571 3762
Size: 84 91 49 1023 613 685 993 191
Cuts at: 3762 33
Size: 1121
Fragments arranged by size:
1121 1023 993 685 613 191 91 84
49
SfoI GGC'GCC
Cuts at: 582 2282 2530 582
Size: 1700 248 2902
Fragments arranged by size:
2902 1700 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SgrAI Cr'CCGG_yG
Cuts at: 476 476
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 156 897 2005 2920 3412 3674 156
Size: 741 1108 915 492 262 1332
Fragments arranged by size:
1332 1108 915 741 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4850
SphI G_CATG'C
Cuts at: 1903 2403 1903
Size: 500 4350
StyI C'CwwG_G
Cuts at: 1143 1984 2338 1143
Size: 841 354 3655
Fragments arranged by size:
3655 841 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 643 1240 2433 2566 3025 3234 580
Size: 63 597 1193 133 459 209 2196
Fragments arranged by size:
2196 1193 597 459 209 133 63
TatI w'GTAC_w
Cuts at: 1044 1383 4365 1044
Size: 339 2982 1529
Fragments arranged by size:
2982 1529 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 316 348 4367 4399 316
Size: 32 4019 32 767
Fragments arranged by size:
4019 767 32 32
Tth111I GACn'n_nGTC
Cuts at: 2647 2647
Size: 4850
XbaI T'CTAG_A
Cuts at: 961 1881 961
Size: 920 3930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 864 1678 2345 864
Size: 814 667 3369
Fragments arranged by size:
3369 814 667
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AvrII BaeI
BfrBI BglI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI