Restriction map for
pF1KB9288
(Circular) (Six-base) MAPSORT of: pF1KB9288.seq Check: 7527 from: 1 to: 6206
pF1KB9288 6206 bp
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 110 110
Size: 6206
AccI GT'mk_AC
Cuts at: 3244 3244
Size: 6206
Acc65I G'GTAC_C
Cuts at: 3222 3222
Size: 6206
AclI AA'CG_TT
Cuts at: 2622 6046 2622
Size: 3424 2782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 264 290 1292 1353 1422 1488 2076 2487
Size: 104 26 1002 61 69 66 588 411
Cuts at: 2487 3135 4031 4463 5210 5478 160
Size: 648 896 432 747 268 888
Fragments arranged by size:
1002 896 888 747 648 588 432 411
268 104 69 66 61 26
AfeI AGC'GCT
Cuts at: 2707 2707
Size: 6206
AflIII A'CryG_T
Cuts at: 1305 1487 2656 3558 4662 5639 1305
Size: 182 1169 902 1104 977 1872
Fragments arranged by size:
1872 1169 1104 977 902 182
AgeI A'CCGG_T
Cuts at: 4594 4594
Size: 6206
AhdI GACnn_n'nnGTC
Cuts at: 1589 1589
Size: 6206
AleI CACnn'nnGTG
Cuts at: 1526 1717 2219 1526
Size: 191 502 5513
Fragments arranged by size:
5513 502 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 904 936 2335 2367 904
Size: 32 1399 32 4743
Fragments arranged by size:
4743 1399 32 32
AlwNI CAG_nnn'CTG
Cuts at: 264 417 1027 1574 3511 5078 264
Size: 153 610 547 1937 1567 1392
Fragments arranged by size:
1937 1567 1392 610 547 153
ApaI G_GGCC'C
Cuts at: 314 547 314
Size: 233 5973
ApaLI G'TGCA_C
Cuts at: 4976 4976
Size: 6206
ApoI r'AATT_y
Cuts at: 1066 1454 1706 1807 2059 2210 3210 5559 5815
Size: 388 252 101 252 151 1000 2349 256
Cuts at: 5815 1066
Size: 1457
Fragments arranged by size:
2349 1457 1000 388 256 252 252 151
101
AseI AT'TA_AT
Cuts at: 20 20
Size: 6206
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6206
AvaI C'yCGr_G
Cuts at: 540 738 753 3110 3226 540
Size: 198 15 2357 116 3520
Fragments arranged by size:
3520 2357 198 116 15
BamHI G'GATC_C
Cuts at: 2771 3231 2771
Size: 460 5746
BanI G'GyrC_C
Cuts at: 749 3222 3636 3884 3919 749
Size: 2473 414 248 35 3036
Fragments arranged by size:
3036 2473 414 248 35
BanII G_rGCy'C
Cuts at: 314 440 547 559 588 1160 1418 2390 2995
Size: 126 107 12 29 572 258 972 605
Cuts at: 2995 3220 4250 314
Size: 225 1030 2270
Fragments arranged by size:
2270 1030 972 605 572 258 225 126
107 29 12
BbeI G_GCGC'C
Cuts at: 3640 3888 3640
Size: 248 5958
BbsI GAAGACnn'nnnn_
Cuts at: 146 296 1531 2894 2972 3152 146
Size: 150 1235 1363 78 180 3200
Fragments arranged by size:
3200 1363 1235 180 150 78
BbvCI CC'TCA_GC
Cuts at: 358 358
Size: 6206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 694 728 90
Size: 34 570 34 5568
Fragments arranged by size:
5568 570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4104 4865 6206 4104
Size: 761 1341 4104
Fragments arranged by size:
4104 1341 761
BclI T'GATC_A
Cuts at: 1860 3726 1860
Size: 1866 4340
BglI GCCn_nnn'nGGC
Cuts at: 2408 2408
Size: 6206
BglII A'GATC_T
Cuts at: 3721 5758 3721
Size: 2037 4169
BlpI GC'TnA_GC
Cuts at: 1004 3318 1004
Size: 2314 3892
Bme1580I G_kGCm'C
Cuts at: 314 547 754 3831 3924 4980 314
Size: 233 207 3077 93 1056 1540
Fragments arranged by size:
3077 1540 1056 233 207 93
BmgBI CAC'GTC
Cuts at: 1488 1488
Size: 6206
BmrI ACTGGGnnnn_n'
Cuts at: 144 3418 3499 3834 5978 144
Size: 3274 81 335 2144 372
Fragments arranged by size:
3274 2144 372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1063 4573 1063
Size: 3510 2696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 200 517 552 676 781 919 1033 1736
Size: 102 317 35 124 105 138 114 703
Cuts at: 1736 2483 2979 2995 98
Size: 747 496 16 3309
Fragments arranged by size:
3309 747 703 496 317 138 124 114
105 102 35 16
Bpu10I CC'TnA_GC
Cuts at: 143 358 418 1107 1510 1851 143
Size: 215 60 689 403 341 4498
Fragments arranged by size:
4498 689 403 341 215 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 3382 4261 4753 5051 1616
Size: 1766 879 492 298 2771
Fragments arranged by size:
2771 1766 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 752 796 1231 1868 2781 5910 752
Size: 44 435 637 913 3129 1048
Fragments arranged by size:
3129 1048 913 637 435 44
BsaAI yAC'GTr
Cuts at: 682 2659 3559 682
Size: 1977 900 3329
Fragments arranged by size:
3329 1977 900
BsaBI GATnn'nnATC
Cuts at: 6 2016 3720 5757 6
Size: 2010 1704 2037 455
Fragments arranged by size:
2037 2010 1704 455
BsaHI Gr'CG_yC
Cuts at: 1761 3637 3885 4587 6083 1761
Size: 1876 248 702 1496 1884
Fragments arranged by size:
1884 1876 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1673 3395 3916 4594 4868 5015 5426 1673
Size: 1722 521 678 274 147 411 2453
Fragments arranged by size:
2453 1722 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 749 779 358
Size: 30 361 30 5785
Fragments arranged by size:
5785 361 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 514 2065 2398 2428 493
Size: 21 1551 333 30 4271
Fragments arranged by size:
4271 1551 333 30 21
BseYI C'CCAG_C
Cuts at: 694 791 3631 4389 4966 5462 694
Size: 97 2840 758 577 496 1438
Fragments arranged by size:
2840 1438 758 577 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 472 581 1587 1765 1932 2502 2879 241
Size: 231 109 1006 178 167 570 377 3568
Fragments arranged by size:
3568 1006 570 377 231 178 167 109
BsiEI CG_ry'CG
Cuts at: 104 3748 3794 5002 104
Size: 3644 46 1208 1308
Fragments arranged by size:
3644 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 2130 2995 3220 3998 4188 4980 440
Size: 1690 865 225 778 190 792 1666
Fragments arranged by size:
1690 1666 865 792 778 225 190
Bsp1286I G_dGCh'C
Cuts at: 314 440 547 559 588 754 1160 1418 2130
Size: 126 107 12 29 166 406 258 712
Cuts at: 2130 2390 2995 3220 3831 3924 3998 4188 4250
Size: 260 605 225 611 93 74 190 62
Cuts at: 4250 4980 314
Size: 730 1540
Fragments arranged by size:
1540 730 712 611 605 406 260 258
225 190 166 126 107 93 74 62
29 12
BspEI T'CCGG_A
Cuts at: 5426 5426
Size: 6206
BspHI T'CATG_A
Cuts at: 5382 5382
Size: 6206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 110 954 3256 3470 3772 4153 110
Size: 844 2302 214 302 381 2163
Fragments arranged by size:
2302 2163 844 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4498 4552 6036 6201 50
Size: 4448 54 1484 165 55
Fragments arranged by size:
4448 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4118 4118
Size: 6206
BsrFI r'CCGG_y
Cuts at: 329 4204 4385 4594 329
Size: 3875 181 209 1941
Fragments arranged by size:
3875 1941 209 181
BsrGI T'GTAC_A
Cuts at: 881 881
Size: 6206
BssSI C'ACGA_G
Cuts at: 4477 4835 4477
Size: 358 5848
BstAPI GCAn_nnn'nTGC
Cuts at: 1910 3583 1910
Size: 1673 4533
BstBI TT'CG_AA
Cuts at: 3163 4567 3163
Size: 1404 4802
BstEII G'GTnAC_C
Cuts at: 828 828
Size: 6206
BstXI CCAn_nnnn'nTGG
Cuts at: 122 122
Size: 6206
BstYI r'GATC_y
Cuts at: 1 386 2771 3231 3698 3721 4056 4302 5314
Size: 385 2385 460 467 23 335 246 1012
Cuts at: 5314 5400 5412 5758 1
Size: 86 12 346 449
Fragments arranged by size:
2385 1012 467 460 449 385 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1010 2318 1010
Size: 1308 4898
BtgI C'CryG_G
Cuts at: 979 979
Size: 6206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4156 4337 4500 4156
Size: 181 163 5862
Fragments arranged by size:
5862 181 163
BtsI GCAGTG_nn'
Cuts at: 1575 2167 3449 1575
Size: 592 1282 4332
Fragments arranged by size:
4332 1282 592
DraI TTT'AAA
Cuts at: 1647 3205 1647
Size: 1558 4648
DraIII CAC_nnn'GTG
Cuts at: 1548 1548
Size: 6206
DrdI GACnn_nn'nnGTC
Cuts at: 3912 4770 3912
Size: 858 5348
EaeI y'GGCC_r
Cuts at: 255 3791 3965 4356 4383 5728 255
Size: 3536 174 391 27 1345 733
Fragments arranged by size:
3536 1345 733 391 174 27
EagI C'GGCC_G
Cuts at: 3791 3791
Size: 6206
EarI CTCTTCn'nnn_
Cuts at: 191 283 1174 1229 2552 2945 4229 4439 191
Size: 92 891 55 1323 393 1284 210 1958
Fragments arranged by size:
1958 1323 1284 891 393 210 92 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 259 4414 4724 4870 6016 259
Size: 4155 310 146 1146 449
Fragments arranged by size:
4155 1146 449 310 146
EcoICRI GAG'CTC
Cuts at: 438 2993 3218 438
Size: 2555 225 3426
Fragments arranged by size:
3426 2555 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 160 200 264 290 517 552 676 781
Size: 62 40 64 26 227 35 124 105
Cuts at: 781 919 1033 1292 1353 1422 1488 1736 2076
Size: 138 114 259 61 69 66 248 340
Cuts at: 2076 2483 2487 2979 2995 3135 4031 4463 5210
Size: 407 4 492 16 140 896 432 747
Cuts at: 5210 5478 98
Size: 268 826
Fragments arranged by size:
896 826 747 492 432 407 340 268
259 248 227 140 138 124 114 105
69 66 64 62 61 40 35 26
16 4
EcoO109I rG'GnC_Cy
Cuts at: 310 311 513 537 544 779 984 3119 3345
Size: 1 202 24 7 235 205 2135 226
Cuts at: 3345 310
Size: 3171
Fragments arranged by size:
3171 2135 235 226 205 202 24 7
1
EcoRI G'AATT_C
Cuts at: 1454 2210 3210 1454
Size: 756 1000 4450
Fragments arranged by size:
4450 1000 756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4225 4225
Size: 6206
FspI TGC'GCA
Cuts at: 3987 3987
Size: 6206
HaeII r_GCGC'y
Cuts at: 2709 3640 3888 4910 5889 2709
Size: 931 248 1022 979 3026
Fragments arranged by size:
3026 1022 979 931 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 511 543 1575 1607 2502 2529 2534
Size: 32 121 32 1032 32 895 27 5
Cuts at: 2534 2561 4048 4078 4080 4110 358
Size: 27 1487 30 2 30 2454
Fragments arranged by size:
2454 1487 1032 895 121 32 32 32
30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 2199 2760 3245 2199
Size: 561 485 5160
Fragments arranged by size:
5160 561 485
HindIII A'AGCT_T
Cuts at: 1751 2978 1751
Size: 1227 4979
HpaI GTT'AAC
Cuts at: 2760 2760
Size: 6206
KasI G'GCGC_C
Cuts at: 3636 3884 3636
Size: 248 5958
KpnI G_GTAC'C
Cuts at: 3226 3226
Size: 6206
MluI A'CGCG_T
Cuts at: 5639 5639
Size: 6206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 944 1709 1782 4877 5061 944
Size: 765 73 3095 184 2089
Fragments arranged by size:
3095 2089 765 184 73
MscI TGG'CCA
Cuts at: 257 3967 257
Size: 3710 2496
MslI CAynn'nnrTG
Cuts at: 603 1122 1355 1388 1526 1563 1647 1717 2151
Size: 519 233 33 138 37 84 70 434
Cuts at: 2151 2219 2478 2573 4195 4322 603
Size: 68 259 95 1622 127 2487
Fragments arranged by size:
2487 1622 519 434 259 233 138 127
95 84 70 68 37 33
MspA1I CmG'CkG
Cuts at: 2134 3317 3631 3991 5004 5249 2134
Size: 1183 314 360 1013 245 3091
Fragments arranged by size:
3091 1183 1013 360 314 245
NaeI GCC'GGC
Cuts at: 331 4387 331
Size: 4056 2150
NarI GG'CG_CC
Cuts at: 3637 3885 3637
Size: 248 5958
NcoI C'CATG_G
Cuts at: 979 979
Size: 6206
NdeI CA'TA_TG
Cuts at: 1938 2036 3052 3179 1938
Size: 98 1016 127 4965
Fragments arranged by size:
4965 1016 127 98
NgoMIV G'CCGG_C
Cuts at: 329 4385 329
Size: 4056 2150
NspI r_CATG'y
Cuts at: 130 996 1309 2302 3259 3759 4666 130
Size: 866 313 993 957 500 907 1670
Fragments arranged by size:
1670 993 957 907 866 500 313
PasI CC'CwG_GG
Cuts at: 2121 2121
Size: 6206
PciI A'CATG_T
Cuts at: 1305 4662 1305
Size: 3357 2849
PfoI T'CCnGG_A
Cuts at: 335 529 797 3011 335
Size: 194 268 2214 3530
Fragments arranged by size:
3530 2214 268 194
PmeI GTTT'AAAC
Cuts at: 3205 3205
Size: 6206
PmlI CAC'GTG
Cuts at: 682 682
Size: 6206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5345 5377 5345
Size: 32 6174
PpuMI rG'GwC_Cy
Cuts at: 513 537 779 513
Size: 24 242 5940
Fragments arranged by size:
5940 242 24
PshAI GACnn'nnGTC
Cuts at: 1764 2111 1764
Size: 347 5859
PsiI TTA'TAA
Cuts at: 1730 1730
Size: 6206
PspOMI G'GGCC_C
Cuts at: 310 543 310
Size: 233 5973
PstI C_TGCA'G
Cuts at: 509 1906 2113 3253 3938 509
Size: 1397 207 1140 685 2777
Fragments arranged by size:
2777 1397 1140 685 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6206
PvuII CAG'CTG
Cuts at: 3631 3991 3631
Size: 360 5846
RsrII CG'GwC_CG
Cuts at: 4401 4401
Size: 6206
SacI G_AGCT'C
Cuts at: 440 2995 3220 440
Size: 2555 225 3426
Fragments arranged by size:
3426 2555 225
SalI G'TCGA_C
Cuts at: 3243 3243
Size: 6206
SanDI GG'GwC_CC
Cuts at: 537 537
Size: 6206
SapI GCTCTTCn'nnn_
Cuts at: 283 1174 4229 4439 283
Size: 891 3055 210 2050
Fragments arranged by size:
3055 2050 891 210
SbfI CC_TGCA'GG
Cuts at: 3253 3253
Size: 6206
ScaI AGT'ACT
Cuts at: 5723 5723
Size: 6206
SexAI A'CCwGG_T
Cuts at: 761 775 761
Size: 14 6192
SfcI C'TryA_G
Cuts at: 33 505 1034 1845 1902 2109 2265 2780 3249
Size: 472 529 811 57 207 156 515 469
Cuts at: 3249 3934 4927 5118 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 811 685 529 515 472 469
207 191 156 57
SfoI GGC'GCC
Cuts at: 3638 3886 3638
Size: 248 5958
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6206
SmaI CCC'GGG
Cuts at: 542 755 3228 542
Size: 213 2473 3520
Fragments arranged by size:
3520 2473 213
SmlI C'TyrA_G
Cuts at: 1631 3110 3361 4276 4768 5030 1631
Size: 1479 251 915 492 262 2807
Fragments arranged by size:
2807 1479 915 492 262 251
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6206
SphI G_CATG'C
Cuts at: 130 2302 3259 3759 130
Size: 2172 957 500 2577
Fragments arranged by size:
2577 2172 957 500
StuI AGG'CCT
Cuts at: 363 2416 363
Size: 2053 4153
StyI C'CwwG_G
Cuts at: 377 979 3340 3694 377
Size: 602 2361 354 2889
Fragments arranged by size:
2889 2361 602 354
SwaI ATTT'AAAT
Cuts at: 1647 1647
Size: 6206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 438 1981 3789 3922 4381 4590 438
Size: 1543 1808 133 459 209 2054
Fragments arranged by size:
2054 1808 1543 459 209 133
TatI w'GTAC_w
Cuts at: 881 5721 881
Size: 4840 1366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 671 703 5723 5755 671
Size: 32 5020 32 1122
Fragments arranged by size:
5020 1122 32 32
Tth111I GACn'n_nGTC
Cuts at: 4003 4003
Size: 6206
XbaI T'CTAG_A
Cuts at: 3237 3237
Size: 6206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 566 3701 566
Size: 3135 3071
XhoI C'TCGA_G
Cuts at: 3110 3110
Size: 6206
XmaI C'CCGG_G
Cuts at: 540 753 3226 540
Size: 213 2473 3520
Fragments arranged by size:
3520 2473 213
XmnI GAAnn'nnTTC
Cuts at: 1169 1377 1669 1753 1921 2500 2818 1169
Size: 208 292 84 168 579 318 4557
Fragments arranged by size:
4557 579 318 292 208 168 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AlfI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI MfeI NheI NotI NruI NsiI
PacI PflMI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI ZraI