Restriction map for
pF1KB9295
(Circular) (Six-base) MAPSORT of: pF1KB9295.seq Check: 3560 from: 1 to: 3722
pF1KB9295 3722 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 671 1547 1979 2726 2994 413
Size: 258 876 432 747 268 1141
Fragments arranged by size:
1141 876 747 432 268 258
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 436 1027 2594 436
Size: 591 1567 1564
Fragments arranged by size:
1567 1564 591
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 726 3075 3331 726
Size: 2349 256 1117
Fragments arranged by size:
2349 1117 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 742 742
Size: 3722
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 256 738 1152 1400 1435 256
Size: 482 414 248 35 2543
Fragments arranged by size:
2543 482 414 248 35
BanII G_rGCy'C
Cuts at: 427 736 1766 427
Size: 309 1030 2383
Fragments arranged by size:
2383 1030 309
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BbsI GAAGACnn'nnnn_
Cuts at: 144 144
Size: 3722
BciVI GTATCCnnnnn_n'
Cuts at: 248 1620 2381 3722 248
Size: 1372 761 1341 248
Fragments arranged by size:
1372 1341 761 248
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 1347 1440 2496 1347
Size: 93 1056 2573
Fragments arranged by size:
2573 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 578 209
Size: 369 3353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 1153 1401 2103 3599 1153
Size: 248 702 1496 1276
Fragments arranged by size:
1496 1276 702 248
BsaWI w'CCGG_w
Cuts at: 911 1432 2110 2384 2531 2942 911
Size: 521 678 274 147 411 1691
Fragments arranged by size:
1691 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1147 1905 2482 2978 1147
Size: 758 577 496 1891
Fragments arranged by size:
1891 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 427 736 1514 1704 2496 427
Size: 309 778 190 792 1653
Fragments arranged by size:
1653 792 778 309 190
Bsp1286I G_dGCh'C
Cuts at: 427 736 1347 1440 1514 1704 1766 2496 427
Size: 309 611 93 74 190 62 730 1653
Fragments arranged by size:
1653 730 611 309 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 986 1288 1669 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 183 1720 1901 2110 183
Size: 1537 181 209 1795
Fragments arranged by size:
1795 1537 209 181
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstYI r'GATC_y
Cuts at: 1 747 1214 1237 1572 1818 2830 2916 2928
Size: 746 467 23 335 246 1012 86 12
Cuts at: 2928 3274 1
Size: 346 449
Fragments arranged by size:
1012 746 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 118 107
Size: 11 3711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1672 1853 2016 1672
Size: 181 163 3378
Fragments arranged by size:
3378 181 163
BtsI GCAGTG_nn'
Cuts at: 477 965 477
Size: 488 3234
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 181 1307 1481 1872 1899 3244 181
Size: 1126 174 391 27 1345 659
Fragments arranged by size:
1345 1126 659 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 432 620 1745 1955 432
Size: 188 1125 210 2199
Fragments arranged by size:
2199 1125 210 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 425 734 425
Size: 309 3413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 413 578 671 1547 1979 2726 2994 209
Size: 204 165 93 876 432 747 268 937
Fragments arranged by size:
937 876 747 432 268 204 165 93
EcoO109I rG'GnC_Cy
Cuts at: 280 861 280
Size: 581 3141
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FseI GG_CCGG'CC
Cuts at: 187 187
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 215 1156 1404 2426 3405 215
Size: 941 248 1022 979 532
Fragments arranged by size:
1022 979 941 532 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1564 1594 1596 1626 1564
Size: 30 2 30 3660
Fragments arranged by size:
3660 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2577 2393
Size: 184 3538
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 1711 1838 1711
Size: 127 3595
MspA1I CmG'CkG
Cuts at: 120 833 1147 1507 2520 2765 120
Size: 713 314 360 1013 245 1077
Fragments arranged by size:
1077 1013 713 360 314 245
NaeI GCC'GGC
Cuts at: 185 1903 185
Size: 1718 2004
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 183 1901 183
Size: 1718 2004
NspI r_CATG'y
Cuts at: 775 1275 2182 775
Size: 500 907 2315
Fragments arranged by size:
2315 907 500
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PfoI T'CCnGG_A
Cuts at: 583 594 583
Size: 11 3711
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PpuMI rG'GwC_Cy
Cuts at: 280 280
Size: 3722
PstI C_TGCA'G
Cuts at: 462 769 1454 462
Size: 307 685 2730
Fragments arranged by size:
2730 685 307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 427 736 427
Size: 309 3413
SacII CC_GC'GG
Cuts at: 121 121
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 432 1745 1955 432
Size: 1313 210 2199
Fragments arranged by size:
2199 1313 210
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SexAI A'CCwGG_T
Cuts at: 146 146
Size: 3722
SfcI C'TryA_G
Cuts at: 33 458 765 1450 2443 2634 33
Size: 425 307 685 993 191 1121
Fragments arranged by size:
1121 993 685 425 307 191
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
StyI C'CwwG_G
Cuts at: 107 856 1210 107
Size: 749 354 2619
Fragments arranged by size:
2619 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 1305 1438 1897 2106 274
Size: 1031 133 459 209 1890
Fragments arranged by size:
1890 1031 459 209 133
TatI w'GTAC_w
Cuts at: 131 308 3237 131
Size: 177 2929 616
Fragments arranged by size:
2929 616 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 3690
Tth111I GACn'n_nGTC
Cuts at: 148 1519 148
Size: 1371 2351
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI