Restriction map for
pF1KB9297
(Circular) (Six-base) MAPSORT of: pF1KB9297.seq Check: 4221 from: 1 to: 4055
pF1KB9297 4055 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 633 633
Size: 4055
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1064 1880 2312 3059 3327 157
Size: 907 816 432 747 268 885
Fragments arranged by size:
907 885 816 747 432 268
AflIII A'CryG_T
Cuts at: 728 1407 2511 3488 728
Size: 679 1104 977 1295
Fragments arranged by size:
1295 1104 977 679
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 417 1360 2927 417
Size: 943 1567 1545
Fragments arranged by size:
1567 1545 943
ApaI G_GGCC'C
Cuts at: 433 433
Size: 4055
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 906 1015 1059 3408 3664 906
Size: 109 44 2349 256 1297
Fragments arranged by size:
2349 1297 256 109 44
AseI AT'TA_AT
Cuts at: 20 901 20
Size: 881 3174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 389 426 1024 1075 389
Size: 37 598 51 3369
Fragments arranged by size:
3369 598 51 37
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 1071 1485 1733 1768 1071
Size: 414 248 35 3358
Fragments arranged by size:
3358 414 248 35
BanII G_rGCy'C
Cuts at: 433 610 1069 2099 433
Size: 177 459 1030 2389
Fragments arranged by size:
2389 1030 459 177
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BbsI GAAGACnn'nnnn_
Cuts at: 165 165
Size: 4055
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 179 1575 179
Size: 1396 2659
BglII A'GATC_T
Cuts at: 758 1570 3607 758
Size: 812 2037 1206
Fragments arranged by size:
2037 1206 812
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 433 1680 1773 2829 433
Size: 1247 93 1056 1659
Fragments arranged by size:
1659 1247 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 396 1267 1348 1683 3827 396
Size: 871 81 335 2144 624
Fragments arranged by size:
2144 871 624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 439 148
Size: 291 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1231 2110 2602 2900 316
Size: 915 879 492 298 1471
Fragments arranged by size:
1471 915 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 731 1408 731
Size: 677 3378
BsaBI GATnn'nnATC
Cuts at: 6 577 806 1569 3606 6
Size: 571 229 763 2037 455
Fragments arranged by size:
2037 763 571 455 229
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 360 1244 1765 2443 2717 2864 3275 360
Size: 884 521 678 274 147 411 1140
Fragments arranged by size:
1140 884 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 342 372 342
Size: 30 4025
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 583
Size: 4055
BseYI C'CCAG_C
Cuts at: 1480 2238 2815 3311 1480
Size: 758 577 496 2224
Fragments arranged by size:
2224 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 610 1069 1847 2037 2829 610
Size: 459 778 190 792 1836
Fragments arranged by size:
1836 792 778 459 190
Bsp1286I G_dGCh'C
Cuts at: 433 610 1069 1680 1773 1847 2037 2099 2829
Size: 177 459 611 93 74 190 62 730
Cuts at: 2829 433
Size: 1659
Fragments arranged by size:
1659 730 611 459 190 177 93 74
62
BspEI T'CCGG_A
Cuts at: 360 3275 360
Size: 2915 1140
BspHI T'CATG_A
Cuts at: 176 3231 176
Size: 3055 1000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 633 1105 1319 1621 2002 633
Size: 472 214 302 381 2686
Fragments arranged by size:
2686 472 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BsrGI T'GTAC_A
Cuts at: 143 702 143
Size: 559 3496
BssSI C'ACGA_G
Cuts at: 565 2326 2684 565
Size: 1761 358 1936
Fragments arranged by size:
1936 1761 358
BstAPI GCAn_nnn'nTGC
Cuts at: 918 1432 918
Size: 514 3541
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 758 1080 1547 1570 1905 2151 3163 3249
Size: 757 322 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 757 467 449 346 335 322 246
86 23 12
BtgI C'CryG_G
Cuts at: 194 627 194
Size: 433 3622
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 857 1054 857
Size: 197 3858
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 197 674 1640 1814 2205 2232 3577 197
Size: 477 966 174 391 27 1345 675
Fragments arranged by size:
1345 966 675 477 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 608 1067 608
Size: 459 3596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 157 439 1064 1880 2312 3059 3327 148
Size: 9 282 625 816 432 747 268 876
Fragments arranged by size:
876 816 747 625 432 282 268 9
EcoO109I rG'GnC_Cy
Cuts at: 414 1194 414
Size: 780 3275
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 194 1489 1737 2759 3738 194
Size: 1295 248 1022 979 511
Fragments arranged by size:
1295 1022 979 511 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 1897 1927 1929 1959 232
Size: 32 1633 30 2 30 2328
Fragments arranged by size:
2328 1633 32 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
HindIII A'AGCT_T
Cuts at: 865 865
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 199 1816 199
Size: 1617 2438
MslI CAynn'nnrTG
Cuts at: 2044 2171 2044
Size: 127 3928
MspA1I CmG'CkG
Cuts at: 380 1166 1480 1840 2853 3098 380
Size: 786 314 360 1013 245 1337
Fragments arranged by size:
1337 1013 786 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 194 194
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PasI CC'CwG_GG
Cuts at: 216 682 216
Size: 466 3589
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PflMI CCAn_nnn'nTGG
Cuts at: 417 749 417
Size: 332 3723
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PsiI TTA'TAA
Cuts at: 824 824
Size: 4055
PspOMI G'GGCC_C
Cuts at: 429 429
Size: 4055
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 610 1069 610
Size: 459 3596
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 596 1098 1783 2776 2967 33
Size: 563 502 685 993 191 1121
Fragments arranged by size:
1121 993 685 563 502 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 331 1210 2125 2617 2879 331
Size: 879 915 492 262 1507
Fragments arranged by size:
1507 915 879 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 194 1189 1543 194
Size: 995 354 2706
Fragments arranged by size:
2706 995 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 415 523 1638 1771 2230 2439 415
Size: 108 1115 133 459 209 2031
Fragments arranged by size:
2031 1115 459 209 133 108
TatI w'GTAC_w
Cuts at: 143 367 515 702 3570 143
Size: 224 148 187 2868 628
Fragments arranged by size:
2868 628 224 187 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 342 374 3572 3604 342
Size: 32 3198 32 793
Fragments arranged by size:
3198 793 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 4055
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI