Restriction map for
pF1KB9339
(Circular) (Six-base) MAPSORT of: pF1KB9339.seq Check: 3347 from: 1 to: 6905
pF1KB9339 6905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1527 2286 1527
Size: 759 6146
AatII G_ACGT'C
Cuts at: 795 2744 795
Size: 1949 4956
AccI GT'mk_AC
Cuts at: 1925 3943 1925
Size: 2018 4887
Acc65I G'GTAC_C
Cuts at: 3921 3921
Size: 6905
AclI AA'CG_TT
Cuts at: 6745 6745
Size: 6905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1917 1929 3025 3503 3786 4730 5162 5515 6177
Size: 12 1096 478 283 944 432 353 662
Cuts at: 6177 1917
Size: 2645
Fragments arranged by size:
2645 1096 944 662 478 432 353 283
12
AflIII A'CryG_T
Cuts at: 2939 3332 4257 6057 6338 2939
Size: 393 925 1800 281 3506
Fragments arranged by size:
3506 1800 925 393 281
AgeI A'CCGG_T
Cuts at: 1759 1759
Size: 6905
AhdI GACnn_n'nnGTC
Cuts at: 2670 3558 2670
Size: 888 6017
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 6873
AlwNI CAG_nnn'CTG
Cuts at: 1720 1830 1971 3138 3642 4210 5648 1720
Size: 110 141 1167 504 568 1438 2977
Fragments arranged by size:
2977 1438 1167 568 504 141 110
ApaI G_GGCC'C
Cuts at: 2616 2616
Size: 6905
ApaLI G'TGCA_C
Cuts at: 5743 5743
Size: 6905
ApoI r'AATT_y
Cuts at: 217 376 590 1330 1726 2893 3909 6258 6514
Size: 159 214 740 396 1167 1016 2349 256
Cuts at: 6514 217
Size: 608
Fragments arranged by size:
2349 1167 1016 740 608 396 256 214
159
AseI AT'TA_AT
Cuts at: 20 20
Size: 6905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6905
AvaI C'yCGr_G
Cuts at: 1052 1528 2086 2427 2516 3146 3925 1052
Size: 476 558 341 89 630 779 4032
Fragments arranged by size:
4032 779 630 558 476 341 89
BamHI G'GATC_C
Cuts at: 3930 3930
Size: 6905
BanI G'GyrC_C
Cuts at: 1038 3483 3921 4335 4583 4618 1038
Size: 2445 438 414 248 35 3325
Fragments arranged by size:
3325 2445 438 414 248 35
BanII G_rGCy'C
Cuts at: 811 860 926 2299 2616 2864 2975 3233 3298
Size: 49 66 1373 317 248 111 258 65
Cuts at: 3298 3919 4949 811
Size: 621 1030 2767
Fragments arranged by size:
2767 1373 1030 621 317 258 248 111
66 65 49
BbeI G_GCGC'C
Cuts at: 1042 3487 4339 4587 1042
Size: 2445 852 248 3360
Fragments arranged by size:
3360 2445 852 248
BbsI GAAGACnn'nnnn_
Cuts at: 704 3018 3901 704
Size: 2314 883 3708
Fragments arranged by size:
3708 2314 883
BbvCI CC'TCA_GC
Cuts at: 269 1370 1713 2562 3818 269
Size: 1101 343 849 1256 3356
Fragments arranged by size:
3356 1256 1101 849 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3531 3565 3531
Size: 34 6871
BciVI GTATCCnnnnn_n'
Cuts at: 4803 5859 6905 4803
Size: 1056 1046 4803
Fragments arranged by size:
4803 1056 1046
BclI T'GATC_A
Cuts at: 4425 4425
Size: 6905
BglI GCCn_nnn'nGGC
Cuts at: 2150 3580 2150
Size: 1430 5475
BglII A'GATC_T
Cuts at: 4420 6457 4420
Size: 2037 4868
BlpI GC'TnA_GC
Cuts at: 603 1228 3052 3227 4017 603
Size: 625 1824 175 790 3491
Fragments arranged by size:
3491 1824 790 625 175
Bme1580I G_kGCm'C
Cuts at: 468 2168 2616 4530 4623 5747 468
Size: 1700 448 1914 93 1124 1626
Fragments arranged by size:
1914 1700 1626 1124 448 93
BmrI ACTGGGnnnn_n'
Cuts at: 815 911 3303 3351 4117 4198 4533 6677 815
Size: 96 2392 48 766 81 335 2144 1043
Fragments arranged by size:
2392 2144 1043 766 335 96 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1857 2913 5272 1857
Size: 1056 2359 3490
Fragments arranged by size:
3490 2359 1056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 457 541 574 1080 1321 1852 2656 3111
Size: 78 84 33 506 241 531 804 455
Cuts at: 3111 3520 379
Size: 409 3764
Fragments arranged by size:
3764 804 531 506 455 409 241 84
78 33
Bpu10I CC'TnA_GC
Cuts at: 269 427 1370 1390 1713 2105 2562 3818 269
Size: 158 943 20 323 392 457 1256 3356
Fragments arranged by size:
3356 1256 943 457 392 323 158 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2698 4081 4960 5674 5972 2698
Size: 1383 879 714 298 3631
Fragments arranged by size:
3631 1383 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 413 1430 6609 413
Size: 1017 5179 709
Fragments arranged by size:
5179 1017 709
BsaAI yAC'GTr
Cuts at: 4258 4258
Size: 6905
BsaBI GATnn'nnATC
Cuts at: 6 4419 6456 6
Size: 4413 2037 455
Fragments arranged by size:
4413 2037 455
BsaHI Gr'CG_yC
Cuts at: 792 1039 1873 2576 2741 3484 4336 4584 5286
Size: 247 834 703 165 743 852 248 702
Cuts at: 5286 6782 792
Size: 1496 915
Fragments arranged by size:
1496 915 852 834 743 703 702 248
247 165
BsaWI w'CCGG_w
Cuts at: 1759 3071 3347 4094 4615 5293 5704 5851 6125
Size: 1312 276 747 521 678 411 147 274
Cuts at: 6125 1759
Size: 2539
Fragments arranged by size:
2539 1312 747 678 521 411 276 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1408 1438 1508 1538 3433 3463 1408
Size: 30 70 30 1895 30 4850
Fragments arranged by size:
4850 1895 70 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 916 959 1772 1903 2031 2244 2515 2534
Size: 462 43 813 131 128 213 271 19
Cuts at: 2534 2668 2872 3301 3835 454
Size: 134 204 429 534 3524
Fragments arranged by size:
3524 813 534 462 429 271 213 204
134 131 128 43 19
BseYI C'CCAG_C
Cuts at: 849 1472 2335 2533 4330 5088 5753 6161 849
Size: 623 863 198 1797 758 665 408 1593
Fragments arranged by size:
1797 1593 863 758 665 623 408 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1984 3525 595
Size: 1389 1541 3975
Fragments arranged by size:
3975 1541 1389
BsiEI CG_ry'CG
Cuts at: 104 4447 4493 5723 104
Size: 4343 46 1230 1286
Fragments arranged by size:
4343 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 860 926 1282 1645 1889 2299 2864 2975 3071
Size: 66 356 363 244 410 565 111 96
Cuts at: 3071 3233 3323 3919 4697 4887 5747 860
Size: 162 90 596 778 190 860 2018
Fragments arranged by size:
2018 860 778 596 565 410 363 356
244 190 162 111 96 90 66
BsmI GAATG_Cn'
Cuts at: 980 2821 980
Size: 1841 5064
BsmBI CGTCTCn'nnnn_
Cuts at: 803 2130 2591 803
Size: 1327 461 5117
Fragments arranged by size:
5117 1327 461
Bsp1286I G_dGCh'C
Cuts at: 468 811 860 926 1282 1645 1889 2168 2299
Size: 343 49 66 356 363 244 279 131
Cuts at: 2299 2616 2864 2975 3071 3233 3298 3323 3919
Size: 317 248 111 96 162 65 25 596
Cuts at: 3919 4530 4623 4697 4887 4949 5747 468
Size: 611 93 74 190 62 798 1626
Fragments arranged by size:
1626 798 611 596 363 356 343 317
279 248 244 190 162 131 111 96
93 74 66 65 62 49 25
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 6905
BspHI T'CATG_A
Cuts at: 3416 5337 3416
Size: 1921 4984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1527 1643 2286 3955 4169 4471 4852 1527
Size: 116 643 1669 214 302 381 3580
Fragments arranged by size:
3580 1669 643 381 302 214 116
BsrBI CCG'CTC
Cuts at: 50 113 1596 2104 5197 5251 6735 6900 50
Size: 63 1483 508 3093 54 1484 165 55
Fragments arranged by size:
3093 1484 1483 508 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 1012 3048 4817 1012
Size: 2036 1769 3100
Fragments arranged by size:
3100 2036 1769
BsrFI r'CCGG_y
Cuts at: 1759 2144 2323 2529 4903 5084 1759
Size: 385 179 206 2374 181 3580
Fragments arranged by size:
3580 2374 385 206 181 179
BssSI C'ACGA_G
Cuts at: 5176 5884 5176
Size: 708 6197
BstAPI GCAn_nnn'nTGC
Cuts at: 3737 4282 3737
Size: 545 6360
BstBI TT'CG_AA
Cuts at: 5266 5266
Size: 6905
BstEII G'GTnAC_C
Cuts at: 1762 1762
Size: 6905
BstXI CCAn_nnnn'nTGG
Cuts at: 396 1180 2343 396
Size: 784 1163 4958
Fragments arranged by size:
4958 1163 784
BstYI r'GATC_y
Cuts at: 1 354 884 2556 2752 2999 3930 4397 4420
Size: 353 530 1672 196 247 931 467 23
Cuts at: 4420 4755 5001 5307 5319 5405 6457 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1672 1052 931 530 467 449 353 335
306 247 246 196 86 23 12
BstZ17I GTA'TAC
Cuts at: 1926 1926
Size: 6905
Bsu36I CC'TnA_GG
Cuts at: 3254 3254
Size: 6905
BtgI C'CryG_G
Cuts at: 938 2318 2342 2745 938
Size: 1380 24 403 5098
Fragments arranged by size:
5098 1380 403 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4855 5036 5199 4855
Size: 181 163 6561
Fragments arranged by size:
6561 181 163
BtsI GCAGTG_nn'
Cuts at: 1282 2103 2610 4148 1282
Size: 821 507 1538 4039
Fragments arranged by size:
4039 1538 821 507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 995 1030 995
Size: 35 6870
DraI TTT'AAA
Cuts at: 3904 3904
Size: 6905
DrdI GACnn_nn'nnGTC
Cuts at: 906 4611 5955 906
Size: 3705 1344 1856
Fragments arranged by size:
3705 1856 1344
EaeI y'GGCC_r
Cuts at: 1464 2321 2487 3458 3530 4490 4664 5055 5082
Size: 857 166 971 72 960 174 391 27
Cuts at: 5082 6427 1464
Size: 1345 1942
Fragments arranged by size:
1942 1345 971 960 857 391 174 166
72 27
EagI C'GGCC_G
Cuts at: 4490 4490
Size: 6905
EarI CTCTTCn'nnn_
Cuts at: 195 618 941 2262 2990 3238 3286 3884 4928
Size: 423 323 1321 728 248 48 598 1044
Cuts at: 4928 5138 195
Size: 210 1962
Fragments arranged by size:
1962 1321 1044 728 598 423 323 248
210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1579 2222 2234 3019 5113 5855 6001 6715 1579
Size: 643 12 785 2094 742 146 714 1769
Fragments arranged by size:
2094 1769 785 742 714 643 146 12
EcoICRI GAG'CTC
Cuts at: 858 924 2297 2862 2973 3231 3917 858
Size: 66 1373 565 111 258 686 3846
Fragments arranged by size:
3846 1373 686 565 258 111 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 457 541 574 1080 1321 1852 1917 1929
Size: 78 84 33 506 241 531 65 12
Cuts at: 1929 2656 3025 3111 3503 3520 3786 4730 5162
Size: 727 369 86 392 17 266 944 432
Cuts at: 5162 5515 6177 379
Size: 353 662 1107
Fragments arranged by size:
1107 944 727 662 531 506 432 392
369 353 266 241 86 84 78 65
33 17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1918 3088 3592 3817 1918
Size: 1170 504 225 5006
Fragments arranged by size:
5006 1170 504 225
EcoO109I rG'GnC_Cy
Cuts at: 1632 2109 2189 2612 2613 2840 3170 3525 3639
Size: 477 80 423 1 227 330 355 114
Cuts at: 3639 3825 4044 1632
Size: 186 219 4493
Fragments arranged by size:
4493 477 423 355 330 227 219 186
114 80 1
EcoRI G'AATT_C
Cuts at: 590 1330 3909 590
Size: 740 2579 3586
Fragments arranged by size:
3586 2579 740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4924 4924
Size: 6905
FspI TGC'GCA
Cuts at: 4686 4686
Size: 6905
HaeII r_GCGC'y
Cuts at: 1042 3487 4339 4587 5817 6588 1042
Size: 2445 852 248 1230 771 1359
Fragments arranged by size:
2445 1359 1230 852 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1234 1266 1408 1440 1606 1638 2656 2688 3544
Size: 32 142 32 166 32 1018 32 856
Cuts at: 3544 3576 4747 4777 4779 4809 1234
Size: 32 1171 30 2 30 3330
Fragments arranged by size:
3330 1171 1018 856 166 142 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 917 1137 1757 2990 3944 917
Size: 220 620 1233 954 3878
Fragments arranged by size:
3878 1233 954 620 220
HindIII A'AGCT_T
Cuts at: 2222 2222
Size: 6905
KasI G'GCGC_C
Cuts at: 1038 3483 4335 4583 1038
Size: 2445 852 248 3360
Fragments arranged by size:
3360 2445 852 248
KpnI G_GTAC'C
Cuts at: 3925 3925
Size: 6905
MfeI C'AATT_G
Cuts at: 503 503
Size: 6905
MluI A'CGCG_T
Cuts at: 6338 6338
Size: 6905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 826 1089 2630 5664 5848 826
Size: 263 1541 3034 184 1883
Fragments arranged by size:
3034 1883 1541 263 184
MscI TGG'CCA
Cuts at: 2489 3460 4666 2489
Size: 971 1206 4728
Fragments arranged by size:
4728 1206 971
MslI CAynn'nnrTG
Cuts at: 985 1178 3191 3358 4894 5021 985
Size: 193 2013 167 1536 127 2869
Fragments arranged by size:
2869 2013 1536 193 167 127
MspA1I CmG'CkG
Cuts at: 268 1248 1452 2004 2010 2566 2573 2747 4016
Size: 980 204 552 6 556 7 174 1269
Cuts at: 4016 4330 4690 5474 5719 268
Size: 314 360 784 245 1454
Fragments arranged by size:
1454 1269 980 784 556 552 360 314
245 204 174 7 6
NaeI GCC'GGC
Cuts at: 2146 5086 2146
Size: 2940 3965
NarI GG'CG_CC
Cuts at: 1039 3484 4336 4584 1039
Size: 2445 852 248 3360
Fragments arranged by size:
3360 2445 852 248
NcoI C'CATG_G
Cuts at: 2318 2318
Size: 6905
NdeI CA'TA_TG
Cuts at: 1179 1179
Size: 6905
NgoMIV G'CCGG_C
Cuts at: 2144 5084 2144
Size: 2940 3965
NspI r_CATG'y
Cuts at: 1812 1824 3336 3735 3765 3958 4458 6061 1812
Size: 12 1512 399 30 193 500 1603 2656
Fragments arranged by size:
2656 1603 1512 500 399 193 30 12
PasI CC'CwG_GG
Cuts at: 1495 2581 1495
Size: 1086 5819
PciI A'CATG_T
Cuts at: 3332 6057 3332
Size: 2725 4180
PflMI CCAn_nnn'nTGG
Cuts at: 1435 2533 3138 3704 1435
Size: 1098 605 566 4636
Fragments arranged by size:
4636 1098 605 566
PfoI T'CCnGG_A
Cuts at: 1282 3083 1282
Size: 1801 5104
PmeI GTTT'AAAC
Cuts at: 3904 3904
Size: 6905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 5351 5383 3380
Size: 32 1939 32 4902
Fragments arranged by size:
4902 1939 32 32
PpuMI rG'GwC_Cy
Cuts at: 1632 2189 2840 3170 3639 1632
Size: 557 651 330 469 4898
Fragments arranged by size:
4898 651 557 469 330
PshAI GACnn'nnGTC
Cuts at: 795 795
Size: 6905
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6905
PspOMI G'GGCC_C
Cuts at: 2612 2612
Size: 6905
PspXI vC'TCGA_Gb
Cuts at: 2427 2427
Size: 6905
PstI C_TGCA'G
Cuts at: 1213 1582 1640 2015 2879 3638 3952 4637 1213
Size: 369 58 375 864 759 314 685 3481
Fragments arranged by size:
3481 864 759 685 375 369 314 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6905
PvuII CAG'CTG
Cuts at: 268 1248 1452 2010 4330 4690 268
Size: 980 204 558 2320 360 2483
Fragments arranged by size:
2483 2320 980 558 360 204
RsrII CG'GwC_CG
Cuts at: 5100 5100
Size: 6905
SacI G_AGCT'C
Cuts at: 860 926 2299 2864 2975 3233 3919 860
Size: 66 1373 565 111 258 686 3846
Fragments arranged by size:
3846 1373 686 565 258 111 66
SacII CC_GC'GG
Cuts at: 2748 2748
Size: 6905
SalI G'TCGA_C
Cuts at: 3942 3942
Size: 6905
SapI GCTCTTCn'nnn_
Cuts at: 3238 3286 4928 5138 3238
Size: 48 1642 210 5005
Fragments arranged by size:
5005 1642 210 48
SbfI CC_TGCA'GG
Cuts at: 3638 3952 3638
Size: 314 6591
ScaI AGT'ACT
Cuts at: 3538 6422 3538
Size: 2884 4021
SfcI C'TryA_G
Cuts at: 33 621 1209 1578 1636 2011 2835 2875 3634
Size: 588 588 369 58 375 824 40 759
Cuts at: 3634 3948 4633 5601 5792 33
Size: 314 685 968 191 1146
Fragments arranged by size:
1146 968 824 759 685 588 588 375
369 314 191 58 40
SfoI GGC'GCC
Cuts at: 1040 3485 4337 4585 1040
Size: 2445 852 248 3360
Fragments arranged by size:
3360 2445 852 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6905
SmaI CCC'GGG
Cuts at: 3148 3927 3148
Size: 779 6126
SmlI C'TyrA_G
Cuts at: 2427 2516 2713 4060 4975 5689 5951 2427
Size: 89 197 1347 915 714 262 3381
Fragments arranged by size:
3381 1347 915 714 262 197 89
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6905
SphI G_CATG'C
Cuts at: 3735 3958 4458 3735
Size: 223 500 6182
Fragments arranged by size:
6182 500 223
StuI AGG'CCT
Cuts at: 2017 2017
Size: 6905
StyI C'CwwG_G
Cuts at: 350 2318 4039 4393 350
Size: 1968 1721 354 2862
Fragments arranged by size:
2862 1968 1721 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 1035 1723 4488 4621 5080 5289 243
Size: 792 688 2765 133 459 209 1859
Fragments arranged by size:
2765 1859 792 688 459 209 133
TatI w'GTAC_w
Cuts at: 2810 2879 3536 6420 2810
Size: 69 657 2884 3295
Fragments arranged by size:
3295 2884 657 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1508 1540 2209 2241 3592 3624 6422 6454 1508
Size: 32 669 32 1351 32 2798 32 1959
Fragments arranged by size:
2798 1959 1351 669 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4702 4702
Size: 6905
XbaI T'CTAG_A
Cuts at: 3936 3936
Size: 6905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 207 393 549 567 1180 1456 3135 3495 4400
Size: 186 156 18 613 276 1679 360 905
Cuts at: 4400 207
Size: 2712
Fragments arranged by size:
2712 1679 905 613 360 276 186 156
18
XhoI C'TCGA_G
Cuts at: 2427 2516 2427
Size: 89 6816
XmaI C'CCGG_G
Cuts at: 3146 3925 3146
Size: 779 6126
XmnI GAAnn'nnTTC
Cuts at: 2941 3442 2941
Size: 501 6404
ZraI GAC'GTC
Cuts at: 793 2742 793
Size: 1949 4956
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BsrGI BssHII ClaI DraIII EcoRV
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PmlI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI