Restriction map for
pF1KB9351
(Circular) (Six-base) MAPSORT of: pF1KB9351.seq Check: 1130 from: 1 to: 8945
pF1KB9351 8945 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5813 5813
Size: 8945
AatII G_ACGT'C
Cuts at: 3048 3048
Size: 8945
AccI GT'mk_AC
Cuts at: 1116 3461 4493 5777 5983 1116
Size: 2345 1032 1284 206 4078
Fragments arranged by size:
4078 2345 1284 1032 206
Acc65I G'GTAC_C
Cuts at: 5961 5961
Size: 8945
AclI AA'CG_TT
Cuts at: 8785 8785
Size: 8945
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 2288 2683 3104 3214 3562 5312 5408 6770
Size: 2014 395 421 110 348 1750 96 1362
Cuts at: 6770 7202 7555 8217 274
Size: 432 353 662 1002
Fragments arranged by size:
2014 1750 1362 1002 662 432 421 395
353 348 110 96
AfeI AGC'GCT
Cuts at: 684 684
Size: 8945
AflII C'TTAA_G
Cuts at: 2425 2425
Size: 8945
AflIII A'CryG_T
Cuts at: 1966 4488 6297 8097 8378 1966
Size: 2522 1809 1800 281 2533
Fragments arranged by size:
2533 2522 1809 1800 281
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 8945
AhdI GACnn_n'nnGTC
Cuts at: 2725 3981 2725
Size: 1256 7689
AleI CACnn'nnGTG
Cuts at: 1612 2993 1612
Size: 1381 7564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3745 4527 4595 3745
Size: 782 68 8095
Fragments arranged by size:
8095 782 68
AlwNI CAG_nnn'CTG
Cuts at: 244 3025 3133 5805 6250 7688 244
Size: 2781 108 2672 445 1438 1501
Fragments arranged by size:
2781 2672 1501 1438 445 108
ApaLI G'TGCA_C
Cuts at: 1186 2568 5655 5710 7783 1186
Size: 1382 3087 55 2073 2348
Fragments arranged by size:
3087 2348 2073 1382 55
ApoI r'AATT_y
Cuts at: 163 1377 2079 2145 2241 2453 4312 4371 4464
Size: 1214 702 66 96 212 1859 59 93
Cuts at: 4464 4576 4839 5405 5635 5857 5949 8298 8554
Size: 112 263 566 230 222 92 2349 256
Cuts at: 8554 163
Size: 554
Fragments arranged by size:
2349 1859 1214 702 566 554 263 256
230 222 212 112 96 93 92 66
59
AseI AT'TA_AT
Cuts at: 20 3184 20
Size: 3164 5781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8945
AvaI C'yCGr_G
Cuts at: 5297 5965 5297
Size: 668 8277
BamHI G'GATC_C
Cuts at: 283 5970 283
Size: 5687 3258
BanI G'GyrC_C
Cuts at: 3656 3760 4928 5961 6375 6623 6658 3656
Size: 104 1168 1033 414 248 35 5943
Fragments arranged by size:
5943 1168 1033 414 248 104 35
BanII G_rGCy'C
Cuts at: 3278 4275 4955 5621 5959 6989 3278
Size: 997 680 666 338 1030 5234
Fragments arranged by size:
5234 1030 997 680 666 338
BbeI G_GCGC'C
Cuts at: 6379 6627 6379
Size: 248 8697
BbsI GAAGACnn'nnnn_
Cuts at: 248 5508 248
Size: 5260 3685
BbvCI CC'TCA_GC
Cuts at: 5722 5722
Size: 8945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 170 204 4705 4739 170
Size: 34 4501 34 4376
Fragments arranged by size:
4501 4376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6843 7899 8945 6843
Size: 1056 1046 6843
Fragments arranged by size:
6843 1056 1046
BclI T'GATC_A
Cuts at: 6465 6465
Size: 8945
BfrBI ATG'CAT
Cuts at: 557 591 2601 4128 557
Size: 34 2010 1527 5374
Fragments arranged by size:
5374 2010 1527 34
BglII A'GATC_T
Cuts at: 2284 4249 6460 8497 2284
Size: 1965 2211 2037 2732
Fragments arranged by size:
2732 2211 2037 1965
BlpI GC'TnA_GC
Cuts at: 2130 5752 6057 2130
Size: 3622 305 5018
Fragments arranged by size:
5018 3622 305
Bme1580I G_kGCm'C
Cuts at: 1190 2572 3441 3659 4869 5029 5659 5714 6570
Size: 1382 869 218 1210 160 630 55 856
Cuts at: 6570 6663 7787 1190
Size: 93 1124 2348
Fragments arranged by size:
2348 1382 1210 1124 869 856 630 218
160 93 55
BmrI ACTGGGnnnn_n'
Cuts at: 1106 1685 1768 2054 2235 3440 5136 6157 6238
Size: 579 83 286 181 1205 1696 1021 81
Cuts at: 6238 6573 8717 1106
Size: 335 2144 1334
Fragments arranged by size:
2144 1696 1334 1205 1021 579 335 286
181 83 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4005 5918 7312 4005
Size: 1913 1394 5638
Fragments arranged by size:
5638 1913 1394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1441 2033 4900 689
Size: 752 592 2867 4734
Fragments arranged by size:
4734 2867 752 592
Bpu10I CC'TnA_GC
Cuts at: 3993 4073 4324 4695 5609 5722 3993
Size: 80 251 371 914 113 7216
Fragments arranged by size:
7216 914 371 251 113 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2751 6121 7000 7714 8012 2751
Size: 3370 879 714 298 3684
Fragments arranged by size:
3684 3370 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 176 5446 8649 176
Size: 5270 3203 472
Fragments arranged by size:
5270 3203 472
BsaAI yAC'GTr
Cuts at: 6298 6298
Size: 8945
BsaBI GATnn'nnATC
Cuts at: 6 6459 8496 6
Size: 6453 2037 455
Fragments arranged by size:
6453 2037 455
BsaHI Gr'CG_yC
Cuts at: 3045 6376 6624 7326 8822 3045
Size: 3331 248 702 1496 3168
Fragments arranged by size:
3331 3168 1496 702 248
BsaWI w'CCGG_w
Cuts at: 920 4904 6134 6655 7333 7744 7891 8165 920
Size: 3984 1230 521 678 411 147 274 1700
Fragments arranged by size:
3984 1700 1230 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 350 380 2413 2443 350
Size: 30 2033 30 6852
Fragments arranged by size:
6852 2033 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1065 2434 3151 3434 4826 1065
Size: 1369 717 283 1392 5184
Fragments arranged by size:
5184 1392 1369 717 283
BseYI C'CCAG_C
Cuts at: 1451 1499 3506 4884 6370 7128 7793 8201 1451
Size: 48 2007 1378 1486 758 665 408 2195
Fragments arranged by size:
2195 2007 1486 1378 758 665 408 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 3229 3283 3954 4592 733
Size: 2496 54 671 638 5086
Fragments arranged by size:
5086 2496 671 638 54
BsiEI CG_ry'CG
Cuts at: 104 6487 6533 7763 104
Size: 6383 46 1230 1286
Fragments arranged by size:
6383 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1167 1190 2049 2572 3104 3278 4453 4834 5659
Size: 23 859 523 532 174 1175 381 825
Cuts at: 5659 5714 5833 5959 6737 6927 7787 1167
Size: 55 119 126 778 190 860 2325
Fragments arranged by size:
2325 1175 860 859 825 778 532 523
381 190 174 126 119 55 23
BsmI GAATG_Cn'
Cuts at: 903 2681 2862 3794 4130 5326 903
Size: 1778 181 932 336 1196 4522
Fragments arranged by size:
4522 1778 1196 932 336 181
Bsp1286I G_dGCh'C
Cuts at: 1167 1190 2049 2572 3104 3278 3441 3659 4275
Size: 23 859 523 532 174 163 218 616
Cuts at: 4275 4453 4834 4869 4955 5029 5621 5659 5714
Size: 178 381 35 86 74 592 38 55
Cuts at: 5714 5833 5959 6570 6663 6737 6927 6989 7787
Size: 119 126 611 93 74 190 62 798
Cuts at: 7787 1167
Size: 2325
Fragments arranged by size:
2325 859 798 616 611 592 532 523
381 218 190 178 174 163 126 119
93 86 74 74 62 55 38 35
23
BspEI T'CCGG_A
Cuts at: 920 920
Size: 8945
BspHI T'CATG_A
Cuts at: 1806 2099 7377 1806
Size: 293 5278 3374
Fragments arranged by size:
5278 3374 293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2394 5813 5995 6209 6511 6892 2394
Size: 3419 182 214 302 381 4447
Fragments arranged by size:
4447 3419 381 302 214 182
BsrBI CCG'CTC
Cuts at: 50 4855 5592 7237 7291 8775 8940 50
Size: 4805 737 1645 54 1484 165 55
Fragments arranged by size:
4805 1645 1484 737 165 55 54
BsrDI GCAATG_nn'
Cuts at: 546 842 2177 3403 4398 4847 5489 6857 546
Size: 296 1335 1226 995 449 642 1368 2634
Fragments arranged by size:
2634 1368 1335 1226 995 642 449 296
BsrFI r'CCGG_y
Cuts at: 4904 6943 7124 4904
Size: 2039 181 6725
Fragments arranged by size:
6725 2039 181
BsrGI T'GTAC_A
Cuts at: 2744 2744
Size: 8945
BssSI C'ACGA_G
Cuts at: 307 3169 3286 7216 7924 307
Size: 2862 117 3930 708 1328
Fragments arranged by size:
3930 2862 1328 708 117
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 3216 4022 4615 6322 1540
Size: 1676 806 593 1707 4163
Fragments arranged by size:
4163 1707 1676 806 593
BstBI TT'CG_AA
Cuts at: 7306 7306
Size: 8945
BstXI CCAn_nnnn'nTGG
Cuts at: 1846 3834 4185 4686 5126 5249 1846
Size: 1988 351 501 440 123 5542
Fragments arranged by size:
5542 1988 501 440 351 123
BstYI r'GATC_y
Cuts at: 1 283 1872 2212 2284 3051 4249 5238 5412
Size: 282 1589 340 72 767 1198 989 174
Cuts at: 5412 5970 6437 6460 6795 7041 7347 7359 7445
Size: 558 467 23 335 246 306 12 86
Cuts at: 7445 8497 1
Size: 1052 449
Fragments arranged by size:
1589 1198 1052 989 767 558 467 449
340 335 306 282 246 174 86 72
23 12
BtgI C'CryG_G
Cuts at: 1916 3764 4679 4946 1916
Size: 1848 915 267 5915
Fragments arranged by size:
5915 1848 915 267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6895 7076 7239 6895
Size: 181 163 8601
Fragments arranged by size:
8601 181 163
BtsI GCAGTG_nn'
Cuts at: 186 1593 3872 6188 186
Size: 1407 2279 2316 2943
Fragments arranged by size:
2943 2316 2279 1407
ClaI AT'CG_AT
Cuts at: 3482 3482
Size: 8945
DraI TTT'AAA
Cuts at: 956 5096 5944 956
Size: 4140 848 3957
Fragments arranged by size:
4140 3957 848
DraIII CAC_nnn'GTG
Cuts at: 2412 5033 5787 2412
Size: 2621 754 5570
Fragments arranged by size:
5570 2621 754
DrdI GACnn_nn'nnGTC
Cuts at: 4602 6651 7995 4602
Size: 2049 1344 5552
Fragments arranged by size:
5552 2049 1344
EaeI y'GGCC_r
Cuts at: 992 3016 3611 4943 6530 6704 7095 7122 8467
Size: 2024 595 1332 1587 174 391 27 1345
Cuts at: 8467 992
Size: 1470
Fragments arranged by size:
2024 1587 1470 1345 1332 595 391 174
27
EagI C'GGCC_G
Cuts at: 6530 6530
Size: 8945
EarI CTCTTCn'nnn_
Cuts at: 935 2951 2969 3350 4862 5508 5857 6968 7178
Size: 2016 18 381 1512 646 349 1111 210
Cuts at: 7178 935
Size: 2702
Fragments arranged by size:
2702 2016 1512 1111 646 381 349 210
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7153 7895 8041 8755 7153
Size: 742 146 714 7343
Fragments arranged by size:
7343 742 714 146
EcoICRI GAG'CTC
Cuts at: 3276 5957 3276
Size: 2681 6264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 689 1441 2033 2288 2683 3104 3214 3562
Size: 415 752 592 255 395 421 110 348
Cuts at: 3562 4900 5312 5408 6770 7202 7555 8217 274
Size: 1338 412 96 1362 432 353 662 1002
Fragments arranged by size:
1362 1338 1002 752 662 592 432 421
415 412 395 353 348 255 110 96
EcoNI CCTnn'n_nnAGG
Cuts at: 292 1178 5182 292
Size: 886 4004 4055
Fragments arranged by size:
4055 4004 886
EcoO109I rG'GnC_Cy
Cuts at: 2056 2506 4630 6084 2056
Size: 450 2124 1454 4917
Fragments arranged by size:
4917 2124 1454 450
EcoRI G'AATT_C
Cuts at: 5635 5949 5635
Size: 314 8631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 704 2182 4841 6964 704
Size: 1478 2659 2123 2685
Fragments arranged by size:
2685 2659 2123 1478
FspI TGC'GCA
Cuts at: 3239 6726 3239
Size: 3487 5458
FspAI rTGC'GCAy
Cuts at: 3239 3239
Size: 8945
HaeII r_GCGC'y
Cuts at: 686 3553 4113 6379 6627 7857 8628 686
Size: 2867 560 2266 248 1230 771 1003
Fragments arranged by size:
2867 2266 1230 1003 771 560 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1862 1894 2197 2229 2643 2675 2711 2743 2767
Size: 32 303 32 414 32 36 32 24
Cuts at: 2767 2799 2885 2917 3030 3062 3163 3195 3225
Size: 32 86 32 113 32 101 32 30
Cuts at: 3225 3257 3967 3999 4320 4352 6787 6817 6819
Size: 32 710 32 321 32 2435 30 2
Cuts at: 6819 6849 1862
Size: 30 3958
Fragments arranged by size:
3958 2435 710 414 321 303 113 101
86 36 32 32 32 32 32 32
32 32 32 32 32 30 30 30
24 2
HincII GTy'rAC
Cuts at: 465 3255 4386 5984 465
Size: 2790 1131 1598 3426
Fragments arranged by size:
3426 2790 1598 1131
HindIII A'AGCT_T
Cuts at: 2309 5835 2309
Size: 3526 5419
KasI G'GCGC_C
Cuts at: 6375 6623 6375
Size: 248 8697
KpnI G_GTAC'C
Cuts at: 5965 5965
Size: 8945
MfeI C'AATT_G
Cuts at: 1313 2573 3322 1313
Size: 1260 749 6936
Fragments arranged by size:
6936 1260 749
MluI A'CGCG_T
Cuts at: 8378 8378
Size: 8945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1166 1697 1863 3418 4077 4274 7704 7888 1166
Size: 531 166 1555 659 197 3430 184 2223
Fragments arranged by size:
3430 2223 1555 659 531 197 184 166
MscI TGG'CCA
Cuts at: 994 3018 3613 6706 994
Size: 2024 595 3093 3233
Fragments arranged by size:
3233 3093 2024 595
MslI CAynn'nnrTG
Cuts at: 203 489 1612 2104 2258 2993 3186 3484 3524
Size: 286 1123 492 154 735 193 298 40
Cuts at: 3524 3769 4183 4684 4934 5482 6934 7061 203
Size: 245 414 501 250 548 1452 127 2087
Fragments arranged by size:
2087 1452 1123 735 548 501 492 414
298 286 250 245 193 154 127 40
MspA1I CmG'CkG
Cuts at: 231 4948 5726 5751 6056 6370 6730 7514 7759
Size: 4717 778 25 305 314 360 784 245
Cuts at: 7759 231
Size: 1417
Fragments arranged by size:
4717 1417 784 778 360 314 305 245
25
NaeI GCC'GGC
Cuts at: 7126 7126
Size: 8945
NarI GG'CG_CC
Cuts at: 6376 6624 6376
Size: 248 8697
NcoI C'CATG_G
Cuts at: 1916 3764 4679 1916
Size: 1848 915 6182
Fragments arranged by size:
6182 1848 915
NdeI CA'TA_TG
Cuts at: 559 559
Size: 8945
NgoMIV G'CCGG_C
Cuts at: 7124 7124
Size: 8945
NsiI A_TGCA'T
Cuts at: 559 593 2603 4130 559
Size: 34 2010 1527 5374
Fragments arranged by size:
5374 2010 1527 34
NspI r_CATG'y
Cuts at: 591 747 3604 4492 5760 5998 6498 8101 591
Size: 156 2857 888 1268 238 500 1603 1435
Fragments arranged by size:
2857 1603 1435 1268 888 500 238 156
PasI CC'CwG_GG
Cuts at: 4011 4011
Size: 8945
PciI A'CATG_T
Cuts at: 4488 8097 4488
Size: 3609 5336
PflMI CCAn_nnn'nTGG
Cuts at: 438 1705 4785 438
Size: 1267 3080 4598
Fragments arranged by size:
4598 3080 1267
PfoI T'CCnGG_A
Cuts at: 1641 2049 1641
Size: 408 8537
PmeI GTTT'AAAC
Cuts at: 5944 5944
Size: 8945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3327 3359 7391 7423 3327
Size: 32 4032 32 4849
Fragments arranged by size:
4849 4032 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 4630 2056
Size: 2574 6371
PsiI TTA'TAA
Cuts at: 449 2117 4408 449
Size: 1668 2291 4986
Fragments arranged by size:
4986 2291 1668
PstI C_TGCA'G
Cuts at: 236 3867 3918 5600 5992 6677 236
Size: 3631 51 1682 392 685 2504
Fragments arranged by size:
3631 2504 1682 685 392 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8945
PvuII CAG'CTG
Cuts at: 231 5751 6370 6730 231
Size: 5520 619 360 2446
Fragments arranged by size:
5520 2446 619 360
RsrII CG'GwC_CG
Cuts at: 7140 7140
Size: 8945
SacI G_AGCT'C
Cuts at: 3278 5959 3278
Size: 2681 6264
SacII CC_GC'GG
Cuts at: 4949 4949
Size: 8945
SalI G'TCGA_C
Cuts at: 5982 5982
Size: 8945
SapI GCTCTTCn'nnn_
Cuts at: 2969 3350 4862 5857 6968 7178 2969
Size: 381 1512 995 1111 210 4736
Fragments arranged by size:
4736 1512 1111 995 381 210
SbfI CC_TGCA'GG
Cuts at: 5992 5992
Size: 8945
ScaI AGT'ACT
Cuts at: 1695 1716 5429 8462 1695
Size: 21 3713 3033 2178
Fragments arranged by size:
3713 3033 2178 21
SexAI A'CCwGG_T
Cuts at: 1595 1595
Size: 8945
SfcI C'TryA_G
Cuts at: 33 232 531 2526 2807 3350 3863 3914 5596
Size: 199 299 1995 281 543 513 51 1682
Cuts at: 5596 5813 5988 6673 7641 7832 33
Size: 217 175 685 968 191 1146
Fragments arranged by size:
1995 1682 1146 968 685 543 513 299
281 217 199 191 175 51
SfoI GGC'GCC
Cuts at: 6377 6625 6377
Size: 248 8697
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8945
SmaI CCC'GGG
Cuts at: 5299 5967 5299
Size: 668 8277
SmlI C'TyrA_G
Cuts at: 2425 2730 6100 7015 7729 7991 2425
Size: 305 3370 915 714 262 3379
Fragments arranged by size:
3379 3370 915 714 305 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8945
SphI G_CATG'C
Cuts at: 747 3604 5760 5998 6498 747
Size: 2857 2156 238 500 3194
Fragments arranged by size:
3194 2857 2156 500 238
SrfI GCCC'GGGC
Cuts at: 5299 5299
Size: 8945
SspI AAT'ATT
Cuts at: 2233 5365 2233
Size: 3132 5813
StuI AGG'CCT
Cuts at: 529 2199 3067 529
Size: 1670 868 6407
Fragments arranged by size:
6407 1670 868
StyI C'CwwG_G
Cuts at: 1740 1916 3701 3764 4679 4791 5183 5735 6079
Size: 176 1785 63 915 112 392 552 344
Cuts at: 6079 6433 1740
Size: 354 4252
Fragments arranged by size:
4252 1785 915 552 392 354 344 176
112 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 729 767 1125 2637 6528 6661 7120 7329 729
Size: 38 358 1512 3891 133 459 209 2345
Fragments arranged by size:
3891 2345 1512 459 358 209 133 38
TatI w'GTAC_w
Cuts at: 1693 1714 1831 1959 2744 4088 5427 8460 1693
Size: 21 117 128 785 1344 1339 3033 2178
Fragments arranged by size:
3033 2178 1344 1339 785 128 117 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 389 421 1583 1615 2562 2594 5155
Size: 32 7 32 1162 32 947 32 2561
Cuts at: 5155 5187 5322 5354 8462 8494 350
Size: 32 135 32 3108 32 801
Fragments arranged by size:
3108 2561 1162 947 801 135 32 32
32 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 1668 6742 1668
Size: 5074 3871
XbaI T'CTAG_A
Cuts at: 5976 5976
Size: 8945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1507 1575 4290 4376 4789 6440 1507
Size: 68 2715 86 413 1651 4012
Fragments arranged by size:
4012 2715 1651 413 86 68
XmaI C'CCGG_G
Cuts at: 5297 5965 5297
Size: 668 8277
XmnI GAAnn'nnTTC
Cuts at: 167 684 2457 2945 167
Size: 517 1773 488 6167
Fragments arranged by size:
6167 1773 517 488
ZraI GAC'GTC
Cuts at: 3046 3046
Size: 8945
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BglI BmgBI BmtI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I CspCI EcoRV
FseI HpaI NheI NotI NruI PacI PmlI PshAI
PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI SwaI
XhoI